Binds antibodies anti idiotypic. Medical search. Frequent questions

A new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1 (Camsap1). (cyphergenomics.com)
A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by b-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). (unime.it)
Optimization and validation of assays for anti-drug antibodies (ADA) is challenging, particularly when the drug itself is a human monoclonal antibody due to the excess of human antibodies in serum and the relatively low immune response. (bio-rad-antibodies.com)
In fact, a generic hybrid LC-MS/MS method utilizing a generic immunocapture capture reagent and a universal peptide from humanized monoclonal antibody, typically from the Fc region, can be utilized for bioanalysis of antibody-based biotherapeutics with a Fc backbone for preclinical applications [4]. (betasciencepress-publishing.com)
Using ELISA, we demonstrated that the recombinant protein specifically interacts with the anti-benzo[a]pyrene monoclonal antibody (mAB B2). (actanaturae.ru)

Fc area binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)3] was designed for immunoglobulin G (IgG) supply into cells. (cyphergenomics.com)
The most commonly associated malignancy, B-cell lymphoma, has shown that anti-idiotypic antibody attached to immunoglobulin on the surface of B-cells causes C1-INH deficiency. (medscape.com)
Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusion. (aai.org)
This complex is arranged in nine subunits (six disulfide-linked dimers of A and B, and three disulfide-linked homodimers of C). C1q has binding sites for antibodies (the heavy chain of IMMUNOGLOBULIN G or IMMUNOGLOBULIN M). The interaction of C1q and immunoglobulin activates the two proenzymes COMPLEMENT C1R and COMPLEMENT C1S, thus initiating the cascade of COMPLEMENT ACTIVATION via the CLASSICAL COMPLEMENT PATHWAY. (lookformedical.com)
Another way of distinguishing antibodies serologically involves an analysis of their idiotypes that may be thought of as phenotypic markers of the variable region genes used to encode the antigen binding (Fab) region of immunoglobulin molecules. (bmj.com)
These have potential use in the therapy of AIDS since they possess both the gp120-binding and HIV-blocking properties of rCD4 as well as the long plasma half-life and Fc receptor-binding functions of immunoglobulin. (bvsalud.org)

Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
Antibodies and antigens fit tightly together because their shape and other surface properties (eg, charge) are complementary. (msdmanuals.com)
The same antibody molecule can cross-react with related antigens if their epitopes are similar enough to those of the original antigen. (msdmanuals.com)
Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
In diseases and conditions in which autoantibodies are generated (e.g., autoimmune disease, cancer), the adaptive immune system escapes "tolerance", the internal checks and balances that would normally prevent the generation of antibodies that bind to self-antigens, and results in chronic inflammation, fibrosis, progressive tissue damage or a combination of the foregoing. (justia.com)
Antibodies are usually defined by the antigens to which they bind. (bmj.com)
However, however the scholarly studies also show that mitochondrial autoimmunity is normally a significant element in breasts cancer tumor inception and development, it isn't the only aspect since there's a multiplex autoantibody profile concentrating on centrosome and stem cell antigens aswell as anti-idiotypic antibodies, disclosing the complicated signaling network involved with breasts carcinogenesis. (eagulf.net)

Anti-idiotypic antibodies and anti-idiotypic antibody mimetics are the specific tools that allow us to monitor therapeutic antibody levels and distribution within PK assays. (avacta.com)
An anti-idiotypic antibody can therefore specifically bind to the idiotope of the target antibody, such as a therapeutic antibody, to act as capture and detection reagents in PK assays. (avacta.com)
With Abveris anti-idiotypic antibody discovery services, you get delivery of ideal anti-idiotypic monoclonal antibodies for PK/ADA assays using proprietary technology and industry-leading workflow. (abveris.com)
Anti-idiotypic antibodies are important downstream reagents for specific drug development assays (e.g. (abveris.com)
The antibodies are ideal for use as surrogate positive controls in anti-drug antibody assays, and for the development of pharmacokinetic bridging ELISAs to measure free drug. (the-scientist.com)
Bio-Rad offers a portfolio of recombinant, monoclonal, nonanimal-derived anti-idiotypic antibodies and drug-target complex binders for the development of highly selective and sensitive assays. (the-scientist.com)
earchers can rely on our antibodies to deliver reproducible results over the lifecycle of their bioanalytical assays" she said. (the-scientist.com)
Over the past decades ligand binding assays were the methods of choice for absolute quantification. (publicatie-online.nl)
Highly specific and sensitive detection antibodies are needed for robust pharmacokinetic (PK) and anti-drug antibody (ADA) assays. (bio-rad-antibodies.com)
Traditionally, ligand binding assays (LBA), primarily enzyme-linked immunosorbent assay (ELISA), have been the gold standard for protein quantification. (betasciencepress-publishing.com)
and to block HLA-I restricted responses anti-HLA-I antibody W6/32 ascites (ATCC) was added at a final dilution of 1 : 40 for 30 min before adding peptides in ELISPOT assays. (peptidesprice.com)

In contrast to monoclonal antibodies produced by hybridoma technology, which may lose the capacity to produce the desired antibody over time or the antibody may undergo unwanted changes, which affect its functionality, recombinant antibodies produced in phage display maintain high standard of specificity and low immunogenicity. (wikipedia.org)
Based on their binding specificity 3 types of anti-idiotypic antibodies can be distinguished, which partially overlap with the previously mentioned formats: the classical ones, a group including Fab fragment antibodies, antibodies binding to idiotope outside of the drug binding site and antibodies, which only bind to the already assembled complex of drug bound to the target. (wikipedia.org)
Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)
High specificity, sensitivity and reproducibility in assay performance are key for any anti-idiotypic reagent, in addition to consistent batch-to-batch control, because any specific anti-idiotypic reagent may be used for several years over the lifetime of the drug evaluation process. (avacta.com)
In this respect, the development of recombinant anti-idiotypic reagents, such as Affimer binders, is ideal, as it allows guided selection to achieve binders with sufficient specificity and sensitivity to be able to identify a biotherapeutic target against a complex background of serum. (avacta.com)
Being able to drive the specificity of the anti-idiotypic binder to the idiotope of the biotherapeutic, in this way, is difficult with the use of traditional animal methods for the generation of these reagents. (avacta.com)
As an alternative to using HIV-1 Env as a vaccine immunogen, we are pursuing a novel vaccine strategy where we generate anti-idiotypic antibodies: antibodies raised in a non-human animal model that bind with high affinity and specificity to bNAb precursors. (washington.edu)
However, this recombinant antibody binds digoxin with decreased affinity and altered specificity relative to native 26-10. (aai.org)
The binding specificity pattern nonetheless is most similar to the H chain donor. (aai.org)

Moreover, in addition to the expansion in innovator and biosimilar therapeutic antibodies, advances in the field of antibody engineering have led to the development of antibody-derived molecules, such as bispecific antibodies and antibody drug conjugates, which are now approaching the clinic for use as antibody drugs. (avacta.com)
Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
Antibody-drug conjugates (ADCs) are multicomponent biotherapeutic drugs designed primarily for cancer therapy. (betasciencepress-publishing.com)
For this purpose, we synthesized carcinogen-protein conjugates and prepared mono- and polyclonal antibodies to benzo[a]pyrene. (actanaturae.ru)

An idiotope is a specific set of unique antigenic determinants, typically found in the variable portion an antibody, that define that particular antibody. (avacta.com)
The affinity purified recombinant species consisting of 26-10 H chain and 40-20 kappa-chain expresses complete 26-10 idiotypic determinants. (aai.org)
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. (lookformedical.com)

To be able to specifically identify the therapeutic antibody, amongst this million-fold excess of IgG, highly sensitive affinity reagents are essential. (avacta.com)
Generic immunocapture reagents such as Protein A or G beads, anti-human Fc or anti-human IgG antibodies can be utilized in a hybrid LC-MS/MS for preclinical evaluations [4]. (betasciencepress-publishing.com)
In the past 12 years a number of idiotypes, recognised by polyclonal reagents and idiotopes, recognised by monoclonal antibodies, on DNA antibodies have been identified (reviewed by Isenberg et al 1 ). (bmj.com)

Furthermore, antibodies against the idiotype of the original image (anti-idiotypic antibody) and therapeutic antibody that blocks the activity of the reagent capture antibody cause falsely low measurements and lastly anti-anti-idiotypic antibodies, which mimic the reagent capture antibody could also cause falsely low measurement. (springeropen.com)
The capture reagent in a hybrid assay may be chosen to compete or not to compete with the binding site of the interfering component, hence allowing the measurement of total and/or active drug. (betasciencepress-publishing.com)

In summary, by hyperimmunization with anti‐IgE mAb we could obtain anti‐Id whose antigen‐binding site is recognized by the mast cell receptor specific to the Fc portion of IgE. (huji.ac.il)
Anti-idiotypic antibodies are antibodies that bind to the antigen-binding site of another antibody or biologic. (abveris.com)

Fab fragment antibodies can be used for detection of not bound drugs or free drugs in the serum. (wikipedia.org)
within serum there is approximately 12mg/ml of our natural IgG antibodies, and most therapeutic antibodies are present only at ng/ml levels. (avacta.com)
The majority of biotherapeutics consist of human or humanised antibodies which are highly homologous to the natural antibodies present in the serum. (avacta.com)
The in vitro selection process for specific Affimer binders includes the ability to deselect against homologous targets and serum constituents, which focuses selection upon the idiotypic region of the biotherapeutic and prevents matrix issues arising during use. (avacta.com)
Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly against the antigen. (aetox.com)
C-reactive protein,mannose-binding lectin, and serum amyloid P component activate complement and act as opsonins. (msdmanuals.com)
Enzyme-linked immunosorbent assay (ELISA) is the gold standard when interrogating serum analytes but even so the challenge with interfering human anti-animal IgG antibodies (HAAA) and heterophilic antibodies can cause unnoticeable false positive or negative results (Willman et al. (springeropen.com)
The antidigoxin antibody 26-10 utilizes a VL sequence highly homologous to that of antibody 40-20, an antidigoxin antibody which uses a different VH gene than does 26-10 and lacks significant reactivity with an anti-26-10 idiotypic serum. (aai.org)
OBJECTIVE To compare the levels of the 9G4 idiotope (9G4 Id) in systemic lupus erythematosus (SLE) patients with a detailed disease activity index, the British Isles Lupus Assessment Group (BILAG) index, and serological parameters of disease activity by ds DNA antibody levels and serum C3 concentrations. (bmj.com)
We have also sought to determine whether the 9G4 Id in the serum samples of lupus patients is present on other antibodies with which it has been associated in other conditions such as anti-myeloperoxidase (MPO) antibodies 5 and cold agglutinins, 6 and have undertaken further absorption experiments. (bmj.com)

Guinea pigs were immunized with purified murine monoclonal antibodies (mAb) that had been found to react with epitopes closely related to the site on the IgE molecule which is recognized by the FcϵR. (huji.ac.il)
It was noted that the ZnT8 Triplemix RBA, detecting conformation-dependent antibodies rather than the typical ELISA linear epitopes, was positive in only 6 of 12 mice. (peptidesprice.com)

Although the current classification of acquired angioedema is being readdressed, in acquired angioedema type I, the associated disorders (usually lymphoproliferative malignancies) produce complement-activating factors, idiotype/anti-idiotype antibodies, or other immune complexes that destroy C1-INH function. (medscape.com)
An anti-idiotypic antibody (anti-ID Ab, see the schematic diagram ) binds to the idiotype of another antibody, usually an antibody drug. (genscriptprobio.com)
An idiotype can be defined as the specific combination of idiotopes present within an antibodies complement determining regions. (genscriptprobio.com)
The fusion progeny (gamma 2a, kappa, lambda 1) which binds digoxin and is idiotype-positive, was selected for kappa loss (resulting in loss of digoxin and idiotype binding) and then fused with a heavy (H) chain loss variant of antidigoxin hybridoma 40-20 (kappa, digoxin nonbinding, idiotype negative). (aai.org)

Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised in opposition to the antiprogesterone mAb DB3 (Ab1) had been used to induce an Ab3 antiprogesterone response in BALB/c mice. (aetox.com)
The objective of this grant is to investigate the relationship between the relative frequencies of target B cells and the binding affinity of the immunogen with the expansion and maturation of the target B cells in polyclonal immune system in vivo. (washington.edu)
GenScript ProBio has experience in developing of highly sensitive and specific polyclonal and monoclonal anti-ID Abs to support your antibody drug development. (genscriptprobio.com)

Reovirus-specific cytolytic T cell lines that have idiotypic receptors recognize anti-idiotypic B cell hybridomas. (umassmed.edu)
Expanded pools of diverse B cell clones compete for antigen and T cell help in a Darwinian process that ultimately selects for B cells with higher affinity receptors which can be secreted as soluble molecules (antibodies). (washington.edu)
Tissue-dependent association of muscarinic acetylcholine receptors with guanine nucleotide-binding regulatory proteins. (aspetjournals.org)
Stereospecificity of opiate receptors in a synchronized culture of NG108-15 cells probed with fluorescent anti-idiotypic antibodies. (aspetjournals.org)
Binding kinetics of quinuclidinyl benzilate and methyl-quinuclidinyl benzilate enantiomers at neuronal (M1), cardiac (M2), and pancreatic (M3) muscarinic receptors. (aspetjournals.org)
A number of reports suggest an association between pathogenicity and the number of β-cell receptors available for EMC-D virus binding. (mun.ca)
FACS analyses of virus binding and anti-EMC virus anti-idiotypic antibody binding to β-cells, showed that cells from SJL/J and C57BL/6J mice contain about the same number of EMC-D virus receptors. (mun.ca)
The molecularly imprinted polymers act as artificial antibodies, artificial receptors, or artificial enzymes with the added benefit over their biological counterparts of being highly durable. (mdpi.com)
Therapy targeted at IgE also interferes with its binding to the low-affinity receptors inhibiting the amplification of the Th(2)-type response. (nih.gov)
Omalizumab, a humanized mAb that binds to the CH3 domain near the binding site for the high-affinity type-I IgE Fc receptors of human IgE, can neutralize free IgE and inhibit the IgE allergic pathway without sensitizing mast cells and basophils. (medscimonit.com)

The "IgE‐like" anti‐Id could be affinity purified from immunosorbents made of the anti‐IgE mAb. (huji.ac.il)
These highly specific and high-affinity recombinant antibodies are suitable for bioanalysis and drug monitoring of daratumumab and its biosimilars. (the-scientist.com)
The new range of anti-daratumumab antibodies comprises three fully human IgG1 inhibitory antibodies with varying levels of affinity. (the-scientist.com)
The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
Conditions necessary for in vitro chain recombination of high affinity (10(9) to 10(12) M-1) antidigoxin monoclonal antibodies resulted in decreased affinity for both intact "native" and chain recombinant molecules. (aai.org)

The most commonly used are the scFv, Fab fragments and bispecific antibodies. (wikipedia.org)
Bispecific antibodies combine two different antigen binding specificities within one molecule. (wikipedia.org)
The bispecific antibodies are used to crosslink the target molecules with two different cells and mediate direct cytotoxicity. (wikipedia.org)

Autoantibodies can bind to basic structural molecules and interfere with the synthesis of structural elements and facilitate the uptake of antigen. (hindawi.com)
Therapeutic antibodies are the fastest growing drug class, with continuous increases in the number of innovator molecules approaching the market. (avacta.com)
Consequently, it is important that the anti-idiotypic antibodies or antibody mimetics used for analysis contain examples of non-neutralising antibodies, to ensures the accurate detection of the target-bound biotherapeutics in addition to free biotherapeutic molecules within any clinical samples. (avacta.com)
The complex formed by the binding of antigen and antibody molecules. (lookformedical.com)
These molecules are comprised of monoclonal antibodies conjugated with a highly cytotoxic agents (aka payload) via synthetic linkers. (betasciencepress-publishing.com)

Anti-idiotypic antibodies can bind to a therapeutic antibody for specific recognition in three different modes. (avacta.com)
1) Binding to the binding site of the therapeutic antibody to inhibit the target binding of the therapeutic antibody. (avacta.com)
2) Binding to a region close to the binding site of the therapeutic antibody without inhibiting target binding. (avacta.com)
3) Binding to a complex of therapeutic antibody and its target antigen. (avacta.com)
Bioanalytical scientists are able to gain different information, such as bound drug or total drug levels, regarding the activity of a therapeutic antibody from each of the different methods of anti-idiotypic antibody binding. (avacta.com)
If the anti-idiotypic antibody binds in a neutralising fashion to the therapeutic antibody paratope, this allows us to measure only the levels of free antibody in a sample. (avacta.com)
Binding to an antibody-antigen complex allows the measurement of only the bound therapeutic antibody within a sample. (avacta.com)

As many of the major blockbuster antibody therapeutics are beginning to come off patent an increase has been observed in the number of biosimilar antibodies under development. (avacta.com)

To determine the binding activity, ELISA assay is routinely performed. (wikipedia.org)

24 Chapter 2 ABSTRACT The promising pipeline of therapeutic monoclonal antibodies (mAbs) demands robust bioanalytical methods with swift development times for pharmacokinetic studies. (publicatie-online.nl)

Recombinant antibodies are antibody fragments produced by using recombinant antibody coding genes. (wikipedia.org)
Recombinant antibodies have many advantages in both medical and research applications, which make them a popular subject of exploration and new production against specific targets. (wikipedia.org)
There are several known formats of recombinant antibodies which are commonly produced. (wikipedia.org)
These are the Fab recombinant antibodies, scFv and diabodies. (wikipedia.org)
The functionality may be enhanced by site-specific chemical modifications, adding a peptide-tag or by fusion with a gene to achieve production of bifunctional recombinant antibodies. (wikipedia.org)
As a member of the anti-idiotypic antibodies, Fab fragment recombinant antibodies bind directly to the paratope of the target antibody. (wikipedia.org)
Along scFv and Fab fragments, diabodies or bispecific recombinant antibodies are the third major format. (wikipedia.org)
The production of recombinant antibodies follows principally similar workflow. (wikipedia.org)

That means that they compete with the drug for binding site and have an inhibitory function. (wikipedia.org)
Differentiation of erythrocyte-(GLUT1), liver-(GLUT2), and adipocyte-type (GLUT4) glucose transporters by binding of the inhibitory ligands cytochalasin B, forskolin, dipyridamole, and isobutylmethylxanthine. (aspetjournals.org)

Anti-idiotypic antibodies bind to a paratope of another specific antibody. (wikipedia.org)
Finally, binding to a region close to the paratope, yet not the paratope itself, allows the measurement of total antibody levels, both free and bound, within a sample. (avacta.com)

Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
However, the production of the required anti-idiotypic antibodies and ligands limits high-throughput method development for sensitive, accurate, and reproducible quantification of therapeutic mAbs. (publicatie-online.nl)

Quantitative bioanalysis of antibody-based biotherapeutics by LC-MS is typically conducted using Immunoaffinity (IA) enrichment coupled with liquid chromatography and tandem mass spectrometry (LC-MS/MS), also known as (aka) hybrid LC-MS/MS [3]. (betasciencepress-publishing.com)

An enzyme linked immunosorbent assay was used to measure the 9G4 Id, and anti dsDNA and anti-myeloperoxidase (MPO) antibodies. (bmj.com)

21:183-87) and monoclonal antibodies (mAbs) as well as drug-linking and drug-releasing properties (Lambert, J. (2005) Curr. (justia.com)

2013 ). Circulating HAAAs can result from both iatrogenic and noniatrogenic causes (Kricka 1999 ), while heterophilic arise in healthy individuals as natural antibodies (Levinson and Miller 2002 ). (springeropen.com)

Daratumumab is an anticancer drug that binds to the CD38 protein overexpressed in multiple myeloma cells, leading to immune-mediated apoptosis of the tumor cell. (the-scientist.com)
Pretreatment of the cells with protease, neuraminidases, or with lectins, showed that the receptor is a protein and that sialic acids are required for virus binding to BMP cells. (mun.ca)
An antibody which binds the isolated protein is also described. (patentsencyclopedia.com)
A 75-kDa protein, enriched in plasmodesmata-rich cell wall extracts from the green alga Chara corallina, was sequenced and found to contain three peptides with similarity to the animal actin-binding protein tropomyosin. (edu.au)
Western blot analysis with anti-tropomyosin antibodies confirmed the identity of this 75-kDa protein as a tropomyosin-like protein and further identified an additional 55-kDa protein, while immunofluorescence microscopy localised the antibodies to plasmodesmata and to the subcortical actin bundles and associated structures. (edu.au)
The anti-tropomyosin antibodies detected a single protein at 42.5 kDa in Arabidopsis thaliana extracts and two proteins at 58.5 and 54 kDa in leek extracts, and these localised to plasmodesmata and the cell plate in A. thaliana and to plasmodesmata in leek tissue. (edu.au)
Tropomyosin is an actin-binding protein thought to be involved in a range of functions associated with the actin cytoskeleton, including the regulation of myosin binding to actin filaments, but to date no tropomyosin-like proteins have been conclusively identified in plant genomes. (edu.au)

they are called variable because the amino acids they contain are different in different antibodies. (msdmanuals.com)
The adaptive immune response produces a diversity of different antibodies (on the order of 10 11 unique sequences in a single individual), each of which is capable of binding to a different target epitope. (justia.com)

Some of the anti‐Id caused RBL degranulation and all of them, like IgE, inhibited the binding of specific anti‐Fc ϵ R mAb to RBL cells. (huji.ac.il)
c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
Right panel: antigen binds to the BCR of antigen-specific B cells and is internalized. (hindawi.com)
Our anti-id guarantee promises delivery of anti-idiotypic antibodies capable of specific binding to target biologic. (abveris.com)
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), a global leader of life science research and clinical diagnostic products, today announced the launch of a range of anti-daratumumab antibodies that are specific for daratumumab (Darzalex) and inhibit the binding of the drug to its target, CD38. (the-scientist.com)
Anti-idiotypic antibody mimicking a T-antigen-specific lectin inhibits human epithelial tumor cell proliferation. (conicet.gov.ar)
We try to work as closely as possible with our customers to understand their specific downstream application in order to generate their perfect anti-ID. (genscriptprobio.com)
Pretreating cells with phospholipases C and D or with enzymes specific for N-linked carbohydrates had no significant effect on virus binding. (mun.ca)
The isolation of antibodies that bind to a specific target, let alone a specific epitope on a known target, is of high interest for the development of new products in therapeutics, diagnostics, and more generally, for research purposes. (justia.com)
We aimed to determine the prevalence and clinical significance of complement-binding donor-specific antibodies (DSA) detected up to 30 years after kidney transplantation. (elsevierpure.com)
Treatment of allergic rhinitis with anti-IgE has been shown to be safe and to reduce specific symptoms. (nih.gov)
Setiyono A , Subangkit M, Marea W, Santi VD, Elvira E, Fadhilah M, Aufa S. 2013.Â Anti Coxiella burnetii Antibody Specific for Q Fever Diagnosis Immunohistochemically in Ruminant.Â Proceeding of International Seminar on the Role of Veterinary Science to Support Millennium Development Goals and the 12th Asian Association of Veterinary Schools Congress. (ipb.ac.id)

The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. (justia.com)

A much more challenging problem in antibody discovery is presented when it is known, or suspected, that an antibody response is present and is biologically relevant, but the identity of the target (and consequently, target epitope) is not known, and could be a single target selected from hundreds to tens of thousands of different targets. (justia.com)

Monoclonal anti-idiotypic antibody blocks viral binding to cells. (umassmed.edu)
Approximately 20-30% of HIV-1 infected individuals generate antibodies capable of neutralizing diverse heterologous viral strains (broadly neutralizing antibodies or bNAbs). (washington.edu)
Yet immunization of humans with recombinant Env leads to the production of antibodies with very narrow breadth of neutralization which fail to block infection of diverse circulating viral isolates. (washington.edu)

The binding site of some anti‐idiotypic antibodies (anti‐Id) can appear as a structural image of the antigen and as such may mimic its biologic activity. (huji.ac.il)

It is clear that for use in clinical settings any anti-idiotypic antibodies or antibody mimetics must fulfil high quality standards. (avacta.com)
The anti-daratumumab antibodies are approved for in vitro research purposes and for commercial applications providing in vitro testing services to support preclinical and clinical drug development. (the-scientist.com)
Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
Purpose To investigate the safety and clinical activity of comprehensive human epidermal growth factor receptor (HER) family receptor inhibition using lumretuzumab (anti-HER3) and pertuzumab (anti-HER2) in combination with paclitaxel in patients with metastatic breast cancer (MBC). (regionh.dk)

Faria M. Addressing Bioanalytical needs of Antibody-based Biotherapeutics by LC-MS. J Appl Bioanal 6(1), 7-11 (2020). (betasciencepress-publishing.com)

The Fc portion of antibodies in immune complexes can be bound by C1q of the classical complement pathway, which eventually leads to the release of C5a and C3a. (hindawi.com)

Another is that HAAAs may diminish the signal by blocking the analyte binding to capture and/or detection antibody producing incorrectly low-test values. (springeropen.com)

We here discuss a proof-of-concept treatment trial with omalizumab in nasal polyposis, which--in case of a positive therapeutic response--would also pave the way for anti-IgE treatment approaches for severe non-atopic lower airway disease. (nih.gov)

Abveris devises an industry-leading anti-idiotypic antibody discovery platform that offers both single B cell screening and integrated hybridoma workflows to significantly de-risk projects and improves the predictability of timelines vs. other discovery workflows. (abveris.com)
Methods for identifying such antibodies have been previously described and include hybridoma approaches, display library approaches, B-cell immortalization, B-cell cloning, and single cell screening methods. (justia.com)
The drug-marked antidigoxin cell line 26-10 (gamma 2a, kappa) and a drug-marked light chain producing variant of antidigoxin hybridoma 45-20 (lambda 1) which lacks both digoxin binding and idiotypy were fused. (aai.org)
Hybridoma-hybridoma fusion provides an alternative to in vitro chain recombination for studying the contribution of chain combinational diversity to antibody diversity, antigen binding, and idiotypy. (aai.org)

Binding of the Fc portion of the antibodies to FcR leads to activation of effector cells and further release of proinflammatory cytokines and proteolytic enzymes, mediators of antibody-dependent cell-mediated cytotoxicity (ADCC). (hindawi.com)

Blocking & non-blocking anti-ids are useful for assay development. (abveris.com)

Abveris develops industry-leading technologies and workflows that will drastically enhance the success of anti-idiotypic antibody discovery campaigns. (abveris.com)
One-stop service from anti-ID Ab discovery to PK and ADA kit development and manufacturing, covering preclinical PK and ADA testing needs once for all. (genscriptprobio.com)

Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS. (lookformedical.com)
The Id is detectable on anti-dsDNA antibodies, though it must also be present on other immunoglobulins whose specificities remain unknown. (bmj.com)

Cysteine engineered anti-MUC16 antibodies are engineered by replacing one or more amino acids of a parent anti-MUC16 antibody with non cross-linked, reactive cysteine amino acids. (justia.com)
The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)
Furthermore, MNRR1 appearance in cells induces an anti-inflammatory phenotype with a decrease in the degrees of dangerous reactive oxygen types (ROS) [4]. (eagulf.net)
Pre-stimulation ex vivo of allogeneic T cells by exposure to MOPC315.BM MM cells in the presence of IL-2, anti-CD3 and anti-CD28 resulted in expansion of the myeloma-reactive T cell TCRVβ 2, 3 and 8.3 subfamilies. (biomedcentral.com)

Through these mechanisms B cells are involved both in autoimmune diseases that are traditionally viewed as antibody mediated and also in autoimmune diseases that are commonly classified as T cell mediated. (hindawi.com)

In-vivo, mAb biotherapeutics can exist in multiple forms i.e. free (bivalently unbound), partially free (monovalently bound) and the fully bound drug (bivalently bound). (betasciencepress-publishing.com)

However, only after 10 immunizations over a period of about 6 months could we detect antibodies that competed efficiently with the binding of IgE to rat basophilic leukemia (RBL) cells. (huji.ac.il)
B cells have antibody-dependent and antibody-independent pathogenic functions. (hindawi.com)
Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
Left panel: antigen bound by antibody is taken up via FcR on APCs such as dendritic cells or macrophages. (hindawi.com)
In this grant, we are investigating a new immunization concept that is based on the utilization of anti-idiotypic antibodies against the germline form of targeted BCRs as tools to specifically expand naïve B cells that express such BCRs, focusing on VRC01-class BCRs. (washington.edu)
Transmembrane or otherwise tumor-associated polypeptides specifically expressed on the surface of cancer cells as compared to normal, non-cancerous cell(s) have been identified as cellular targets for cancer diagnosis and therapy with antibodies. (justia.com)
Characterization of angiotensin II (AT2) binding sites in R3T3 cells. (aspetjournals.org)
4 X l0⁵ sites/cell) binding site, Hill analysis of the saturation binding data strongly suggested that EMC-D virus has more than one binding site on BMP cells. (mun.ca)
It is concluded that the receptor for EMC-D virus on BMP cells is a sialylated glycoprotein, and virus binding involves Neu5Ac, and possibly Neu5Gc, but not N-linked sugars. (mun.ca)
Monoclonal antibodies raised against the putative receptor blocked EMC-D virus infection of BMP cells and virus binding to β-cells from SJL/J mice. (mun.ca)
The method entails optionally subjecting a sample to conditions suitable for expansion of one or more of the lymphocytes to form an expanded sample comprising antibody secreting cells (ASCs) and distributing the sample into containers to provide a plurality of subsamples. (justia.com)
T cells are involved in cell-mediated immunity whereas B cells are primarily responsible for humoral immunity (relating to antibodies). (wellnessadvantage.com)
B cells respond to pathogens by producing large quantities of antibodies which then neutralize foreign objects like bacteria and viruses. (wellnessadvantage.com)
MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of binding to b-1,3- glucan. (unime.it)

Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody drug immunogenicity, anti-drug antibody (ADA) studies and neutralization antibody measurement. (genscriptprobio.com)

We raised anti‐anti‐IgE antibodies in an attempt to obtain anti‐Id capable of interacting with the Fcϵ receptor (FcϵR). (huji.ac.il)
One of the first critical steps in an effective antibody response is the recognition of a foreign antigen by a membrane anchored B cell receptor (BCR) on the surface of a naïve B cell. (washington.edu)
Tienen un empleo potencial en el tratamiento del SIDA ya que poseen propiedades de unión a gp120 y de bloqueo del VIH de rCD4 así como una gran semivida plasmática y funcioones de unión al receptor Fc de la inmunoglobulina. (bvsalud.org)

The actin cytoskeleton and associated actin-binding proteins form a complex network involved in a number of fundamental cellular processes including intracellular trafficking. (edu.au)
In plants, both actin and myosin have been localised to plasmodesmata, and thus it is likely that other actin-binding proteins are also associated with plasmodesmata structure or function. (edu.au)

Anatomy19

Organisms17

Diseases7

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes54

Disciplines and Occupations3

Information Science3

Health Care4

Monoclonal antibody5

Immunoglobulin6

Antigens7

Assays11

Specificity9

Conjugates6

Determinants3

Reagents3

Reagent2

Antigen‐bi2

Serum10

Epitopes2

Idiotype4

Polyclonal3

Receptors10

Affinity5

Bispecific antibodies3

Molecules5

Therapeutic antibody7

Biosimilar antibodies1

ELISA1

Pharmacokinetic1

Recombinant antibodies8

Inhibitory2

Paratope2

Ligands2

Bioanalysis1

Enzyme-linke1

MAbs1

Natural antibodies1

Protein7

Different antibodies2

Specific12

Relates to methods2

Epitope1

Viral3

Mimic1

Clinical4

Bioanalytical1

Immune complexes1

Detection1

Omalizumab1

Hybridoma4

Cytotoxicity1

Assay development1

Discovery2

Immunoglobulins2

Reactive4

Autoimmune1

Biotherapeutics1

Cells14

Measurement1

Receptor3

Proteins2