Binding sequence virus mutants. Medical search. Frequent questions

Analysis of the structure and sequence of several different env genes suggests that Env proteins are type 1 fusion machines. (wikipedia.org)
The Env proteins of the Avian Sarcoma and Leukosis virus (ASLV) and the Murine Leukemia Virus (MLV) are both trimers of SU-TM heterodimers. (wikipedia.org)
SV40, known as a DNA virus, binds to tumor suppressing proteins and changes the DNA sequence. (sunridgemedical.com)
The researchers began by homing in on the parts of the coronavirus genomes that encode the spike proteins that give this family of viruses their distinctive crown-like appearance. (nih.gov)
In the first scenario, as the new coronavirus evolved in its natural hosts, possibly bats or pangolins, its spike proteins mutated to bind to molecules similar in structure to the human ACE2 protein, thereby enabling it to infect human cells. (nih.gov)
Scientists have identified a small family of lab-made proteins that neutralize a broad range of influenza A viruses, including the H5N1 avian virus, the 1918 pandemic influenza virus and seasonal H1N1 flu viruses. (science20.com)
These human monoclonal antibodies, identical infection-fighting proteins derived from the same cell lineage, also were found to protect mice from illness caused by H5N1 and other influenza A viruses. (science20.com)
HA and neuraminidase are the two main surface proteins on the influenza virus. (science20.com)
Influenza A viruses can include any one of the 16 known subtypes of HA proteins, which fall into two groups, Group 1 and Group 2. (science20.com)
31). Vif targets APOBEC3G for proteasomal degradation by binding a complex of 3 human proteins called Cullin5, Elongin B, and Elongin C. The phosphorylation of a single amino acid (Ser 144) in Vif regulates this binding. (natap.org)
Although these proteins are likely to have multiple functions, blocking the growth of incoming viruses appears to be among them. (natap.org)
Although the exact mechanism by which TRIM5a proteins inhibit retroviral replication is yet unknown, they block an early step of post-entry infection, likely uncoating of the incoming virus. (natap.org)
The granules, composed of ribonucleic acid-binding protein molecules such as Ras-GTPase-activating SH3 domain-binding-proteins 1 and 2 (G3BP1/2) and ubiquitin-associated protein 2 (UBAP2L), are essential for antiviral signaling. (worldmedicinefoundation.com)
The RNA-binding protein FMR1 and the closely related FXR1 and FXR2 proteins were the most notable cellular host factors co-purifying with NSP3. (worldmedicinefoundation.com)
To investigate the emergence of compensatory mutations in the viral proteins during cell passaging, the consensus sequences of the entire polyprotein coding regions of the wild type JFH1 and three chimeric viruses were determined. (monash.edu)
In summary, this thesis demonstrates the importance of functional interactions between HCV proteins during virus morphogenesis and infectivity. (monash.edu)
Also cleaves Nef and Vif, probably concomitantly with viral structural proteins on maturation of virus particles (By similarity). (proteopedia.org)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

Sequence analysis of the viral capsid protein genes revealed the presence of seven missense mutations, three of which were also present in a second PVpi, suggesting that they could be important determinants of the persistent phenotype. (pasteur.fr)
When the three mutations were introduced into the lytic Leon strain separately, in pairs or all together, all but one of the viruses was capable of establishing a persistent infection. (pasteur.fr)
Early stage computational and experimental studies on potential therapeutics have concentrated on the receptor binding domain, although this region is prone to mutations with the possibility of giving rise to widespread drug resistance. (biorxiv.org)
Here, using atomistic molecular dynamics simulation, we study the correlations between the RBD dynamics with physically distant residues in the spike protein, and provide a deeper understanding of their role in the infection, including the prediction of important mutations and of distant allosteric binding sites for therapeutics. (biorxiv.org)
Significant research effort for drug design against COVID-19 is focused on the receptor binding domain of the spike protein, although this region is prone to mutations causing resistance against therapeutics. (biorxiv.org)
The concept of mutation was coined by Hugo De Vries in 1901, whom worked with plants species of the genus Oenothera where he discovered some phenotypic hereditary characteristics that he coined as "mutations" and "mutants" to those individuals that have these phenotypic alterations. (intechopen.com)
Sequence analysis revealed mutations in core, NS2, NS5A and NS5B. (monash.edu)
Sequencing of cDNA obtained from reverted virions revealed mutations in E1 (I262L) and E2 (N415D). (monash.edu)
These data suggest that mutations within E1 or E2 can compensate for the reduction in infectivity observed for HVR1 deleted viruses. (monash.edu)
Site-directed mutagenesis was used to introduce most of the possible intermediate mutations in the envelope for four distinct coreceptor switch mutants, each with a unique pattern of CCR5 and CXCR4 utilization that extended from highly efficient use of both coreceptors to sole use of CXCR4. (unige.ch)
Coreceptor switch intermediates displayed increased sensitivity to CCR5 inhibitors (except for a few envelopes with mutations in V2 or C2) that correlated with a loss in CCR5 binding. (unige.ch)
Preventive vaccination has historically been the most efficient measure of influenza control, but this approach presents important limitations due to the accumulation of antigenic mutations in the virus, known as antigenic drift. (distantreader.org)
Viruses like SARS-CoV-2 continuously evolve as changes in the genetic code (caused by genetic mutations or viral recombination) occur during replication of the genome. (cdc.gov)
Mutations happen frequently, but only sometimes change the characteristics of the virus. (cdc.gov)
Identification of Bruton tyrosine kinase mutations in 12 Chinese patients with X-linked agammaglobulinaemia by long PCR-direct sequencing. (lu.se)
La présente étude visait à déterminer la prévalence des infections occultes par le virus de l'hépatite B chez des patients égyptiens atteints d'une hépatite C chronique, le génotype et la survenue des mutations génétiques du virus de l'hépatite B et l'impact d'une coïnfection par le virus de l'hépatite B sur la réponse précoce du patient au traitement. (who.int)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
We further explored the cancer-promoting mechanism of target genes in ESCC by sequencing of the genes' exons. (hindawi.com)
We will do this by sequencing the spike (or other receptor binding/fusion) protein genes from all our bat-CoVs, creating mutants to identify how significantly each would need to evolve to use ACE2, CD26/DPP4 (MERS-CoV receptor) or other potential CoV receptors. (substack.com)
Wild-type (WT) viruses demonstrated a 0.50 to 1.30 log drop in SARS-CoV-2 titer after interferon-stimulated genes (ISG) activation in comparison to untreated. (worldmedicinefoundation.com)
The diminished capability of non-structural protein 3 mutants in interferon-deficient VeroE6 cells indicated that IFN-stimulated genes (ISGs) may not be responsible for attenuation. (worldmedicinefoundation.com)
Using a genetic complementation assay to identify additional genes which inhibit apoptosis during infection with a p35 mutant, we have isolated a gene from Orgyia pseudotsugata NPV (OpMNPV) that was able to functionally substitute for AcMNPV p35. (embl.de)

This could also explain why they did not detect so-called escape mutants, viruses that elude the monoclonal antibodies through genetic mutation. (science20.com)

The scientists also identified a new mechanism of antibody action against influenza: Once the antibody binds, the virus cannot change its shape, a step required before it can fuse with and enter the cell it is attempting to infect. (science20.com)
The antibody binds the viral spike protein with high affinity and has demonstrated potent neutralization of live virus infections in vitro and in vivo . (hifibio.com)

Exposed on the surface of the viral envelope, the glycoprotein gp120 binds to the CD4 receptor on any target cell that has such a receptor, particularly the helper T-cell. (wikipedia.org)
An atomic-detail model of the Marburg virus glycoprotein in complex with a neutralizing human monoclonal antibody designated MR78 was constructed using Phenix.Rosetta starting from a 3.6Å crystallographic density map. (elsevierpure.com)
This inter-CDR loop interaction stabilizes the bulged conformation needed for binding to the viral glycoprotein: a Tyr to Phe mutant displays a binding affinity reduced by a factor of at least 10. (elsevierpure.com)
have discovered that the conformation of the human antibody MR78 HCDR3 loop that binds to Marburg virus glycoprotein is stabilized by a non-local hydrogen bond between an Asp at T6 position of HCDR3 and a Tyr in HCDR1. (elsevierpure.com)
The humoral immune response plays an important role in the defense against these viruses, providing protection mainly by producing antibodies directed against the hemagglutinin (HA) glycoprotein. (distantreader.org)

We will then use receptor-mutant pseudovirus binding assays, in vitro studies in bat, primate, human and other species' cell lines, and with humanized mice where particularly interesting viruses are identified phylogenetically, or isolated. (substack.com)
Polyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: requirement for 5' conserved sequences. (ox.ac.uk)
Here we report the development of two independent assays which demonstrate for the first time that exogenous model RNA templates based on influenza virus virion RNA (vRNA) are transcribed in vitro to produce polyadenylated mRNA. (ox.ac.uk)
SARS-CoV-2 NSP3 mutants that prevented FMRP binding slowed proliferation in vitro and delayed illness onset in animals. (worldmedicinefoundation.com)
A mutation that diminished Munc18 binding to syntaxin1 in vitro attenuated the vesicle-docking step but rescued vesicle priming in excess of docking. (jneurosci.org)
His work deals with themes such as Biopanning, In vitro, Gene expression, Recombinant virus and Receptor, which intersect with Molecular biology. (research.com)

The herpes simplex virus DNA polymerase is composed of two subunits, a large catalytic subunit (Pol) and a smaller subunit (UL42) that increases the processivity of the holoenzyme. (ed.ac.uk)
Antiviral agents include acyclovir, foscarnet, or ganciclovir for varicella-zoster virus (VZV), herpes simplex virus (HSV), and cytomegalovirus (CMV). (medscape.com)

The functional importance of the interaction was confirmed by the finding that a pol mutant defective for UL42 binding retained polymerase activity, but did not synthesize longer DNA products in the presence of UL42. (ed.ac.uk)
We investigated the activities of mutated templates with known polymerase binding properties to test our model that polyadenylation occurs when a polymerase complex, which is bound to conserved 5' sequences of vRNA, prevents read-through of the U track at which polyadenylation subsequently occurs by reiterative copying. (ox.ac.uk)
U point mutant at the fourth residue, which retained polymerase binding properties similar to those of the wild type, produced polyadenylated transcripts. (ox.ac.uk)
Our results show that nucleotides within the conserved 5' sequence are required for polyadenylation and support the hypothesis that polymerase binding to 5' sequences of the template is required for mRNA synthesis. (ox.ac.uk)
Analysis of site mutants showed that the region targeted by the polymerase protein contained the signal transducer and activator of transcription (Stat) binding site. (edu.au)

The nucleotide sequence of this gene, Op-iap, predicted a 30-kDa polypeptide product with approximately 58% amino acid sequence identity to the product of CpGV iap, Cp-IAP. (embl.de)
A nucleotide sequence comparison revealed that the MCF13 MLV LTR contains sequences highly homologous to the protein-binding sites identified in the Mo-MLV LTR. (archive.org)

We will test our models of host range (i.e. emergence potential) experimentally using reverse genetics, pseudovirus and receptor binding assays, and virus infection experiments in cell culture and humanized mice. (substack.com)
With bat-CoVs that we've isolated or sequenced, and using live virus or pseudovirus infection in cells of different origin or expressing different receptor molecules, we will assess potential for each isolated virus and those with receptor binding site sequence, to spill over. (substack.com)
Virus infection is initiated by the interaction between S protein and host cell surface receptors. (newvita.com)
After 2.5 months of persistent infection in human neuroblastoma cells by the type 3 poliovirus (PV3) wild-type Leon strain, a mutant (PVpi), L2-2, capable of establishing a persistent infection in nonneural HEp-2c cells was isolated. (pasteur.fr)
Infection of human cells by the novel coronavirus (SARS-Cov-2) involves the attachment of the receptor binding domain (RBD) of the spike protein to the peripheral membrane ACE2 receptors. (biorxiv.org)
We applied time-independent component analysis (tICA) and protein connectivity network model, on all-atom molecular dynamics trajectories, to identify key non-RBD residues, playing crucial role in the conformational transition facilitating spike-receptor binding and infection of human cell. (biorxiv.org)
The viral mutants were weakened in proliferation after 24 hours of infection compared to the SARS-CoV-2 wild-type strain in VeroE6 cells. (worldmedicinefoundation.com)
As use of CXCR4 improved, infection mediated by the mutated envelopes became more resistant to soluble CD4 inhibition and direct binding to CD4 increased. (unige.ch)
ABSTRACT Chronic hepatitis C virus (HCV) infection combined with occult hepatitis B virus (HBV) infection has been associated with increased risk of hepatitis, cirrhosis and hepatocellular carcinoma. (who.int)
RÉSUMÉ L'infection chronique par le virus de l'hépatite C associée à une infection occulte par le virus de l'hépatite B a été associée à un risque accru d'autres hépatites, de cirrhose et de carcinome hépatocellulaire. (who.int)

The C-terminal domain (CTD) of S1 specifically binds to host cell receptors angiotensin-converting enzyme 2 (ACE2) or CD147, which causes the conformational change of S2. (newvita.com)
The HCV glycoproteins E1 and E2 are present on the surface of virions as a heterodimer that attach virions to host cell receptors and facilitate virus fusion and entry. (monash.edu)

By modeling and simulating neuraminidase apo glycoproteins embedded in complex-mixture lipid bilayers, we show that the geometry and dynamics of the neuraminidase enzymatic pocket may differ depending on stalk length, with possible repercussions on the binding of the endogenous sialylated-oligosaccharide receptors. (nih.gov)
Evidence of the long battle of the mammalian cell to protect its genetic material from the onslaught of viral parasites is the existence of hundreds of endogenous retroviruses within the human genome, archaic viral sequences which no longer produce viruses but continue to be handed down from generation to generation. (natap.org)
Ectopic or endogenous VCP stabilized p53-R273H by binding to MDM2 and disrupting its association with mutant p53. (aacrjournals.org)
However, the use of mutant expression in the presence of endogenous protein left it unclear how the observed effects were related to the defect of vesicle docking in (M)unc18-1 knock-outs. (jneurosci.org)

Of 35 respiratory samples tested, 6 were infected with transcription PCR and single nucleotide polymorphism influenza A(H3N2), 2 with influenza A(H1N1) virus, 6 probestodifferentiatethismutantstraininmixedviruspop- with influenza A(H1N1)pdm09 virus, 4 with parainfluenza ulationsinhumanspecimens. (cdc.gov)
Burioni, Roberto title: A Non-VH1-69 Heterosubtypic Neutralizing Human Monoclonal Antibody Protects Mice against H1N1 and H5N1 Viruses date: 2012-04-04 journal: PLoS One DOI: 10.1371/journal.pone.0034415 sha: 6b51562f63de5739f2b7ebf5f9c34365ac6ee545 doc_id: 807 cord_uid: fcffl6m4 Influenza viruses are among the most important human pathogens and are responsible for annual epidemics and sporadic, potentially devastating pandemics. (distantreader.org)
Furthermore, we describe its protective activity in mice after lethal challenge with H1N1 and H5N1 viruses suggesting a potential application in the treatment of influenza virus infections. (distantreader.org)
The haemagglutinin (HA) segments of A(H1N1)pdm09 from both real-time RT-PCR positive and negative samples were isolated and sequenced. (who.int)
Sequencing was conducted on 23 influenza (H1N1)pdm09 isolates that were recovered from positive samples. (who.int)
Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays. (who.int)
A(H1N1)pdm02 became endemic, co-circulating with phase of the reaction, and quality of amplification can be influenza A(H3N2) and B viruses. (who.int)
2017 to March 2018, there was circulation of both detect a single target in a very small concentration of DNA influenza A and B, with influenza A(H1N1)pdm09 again or RNA because it uses a fluorescent dye that binds to predominating in Viet Nam. (who.int)

DNA or RNA purified by MagPure is ready-to-use for all kinds of molecular biology applications such as PCR, sequencing, blotting procedures, mutant analysis and SNP. (bigfishgene.com)
Pierre Boulanger spends much of his time researching Molecular biology, Recombinant DNA, Virus, Virology and Capsid. (research.com)
His work carried out in the field of Molecular biology brings together such families of science as Plasma protein binding, Enzyme, Luciferase, Transduction and Cytotoxicity. (research.com)

However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)

Feng et al established in 2008 that approximately 80% of Merkel cell carcinomas in the Northern hemisphere occur after genomic integration by a ubiquitous skin commensal virus, Merkel cell polyoma virus (MCPyV). (medscape.com)
Assessing genomic variability of SARS-CoV-2 presents a moderate mutation rate compared to other RNA viruses (around 1.12 × 10 −3 nucleotide substitution/site/year), 5 which is at the same level as SARS-CoV-1. (biorxiv.org)
POL_HV1B5 Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5'-UTR. (proteopedia.org)
Biotech Europe GmbH, Freiburg, Germa- cation of the virus in cells heterozygous for ny) was used to extract genomic DNA the mutation proceeded at an intermediate from white blood cells following the lysis rate [ 5 ]. (who.int)

There were two different mismatches in the probe target sites of the HA gene sequences of all isolates ( n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT-PCR isolates. (who.int)

Here, we demonstrate through a combination of insertion and deletion mutagenesis that the carboxy-terminal 35 amino acids of Pol are crucial for binding UL42. (ed.ac.uk)
Deletion of HVR1 increases CD81-binding ability of the mutant and also increases its susceptibility to neutralizing antibody MAb 24. (monash.edu)
Its deletion from the MCF13 virus dramatically reduced the incidence of thymic lymphoma and increased the latency of disease in comparison with the wild-type virus. (archive.org)
A newly identified protein-binding site (MLPal) located within DEN affects transcription only in T cells, and its deletion attenuates the ability of an MCF13 virus with a single enhancer repeat to induce thymic lymphoma. (archive.org)

Transcription of simian virus 40. (cshlpress.com)
This gene encodes a nuclear polyglutamine-binding protein that is involved with transcription activation. (nih.gov)
Here we show that the maize dominant Barren inflorescence3 ( Bif3 ) mutant harbors a tandem duplicated copy of the ZmWUS1 gene, ZmWUS1-B , whose novel promoter enhances transcription in a ring-like pattern. (nature.com)
Overexpression of ZmWUS1-B is due to multimerized binding sites for type-B RESPONSE REGULATORs (RRs), key transcription factors in cytokinin signaling. (nature.com)
We have identified nucleotide sequences that regulate transcription in both a cell-type-specific and general manner in the long terminal repeat of the MCF13 murine leukemia virus. (archive.org)
This observation suggests that the MLPal protein-binding site contributes to the effect of the DEN region on T-cell-specific transcription and viral leukemogenicity. (archive.org)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Hepatitis C virus (HCV) is a small enveloped virus with a positive-sense single stranded RNA genome. (monash.edu)

SARS-CoV-2 is a spherical, enveloped, single-stranded positive RNA virus with a diameter of 80 nm-160 nm and a genome size of 29.9 kb [ 8 ]. (newvita.com)
It binds in the cytoplasm the human BAF protein which prevent autointegration of the viral genome, and might be included in virions at the ration of zero to 3 BAF dimer per virion. (proteopedia.org)
Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that converts the viral RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell. (proteopedia.org)
Genome-wide single-nucleotide polymorphism analysis in juvenile myelomonocytic leukemia identifies uniparental disomy surrounding the NF1 locus in cases associated with neurofibromatosis but not in cases with mutant RAS or PTPN11. (lu.se)

Ribbon diagram of the influenza virus H5 hemagglutinin (HA) surface protein bound by the F10 monoclonal antibody (red). (science20.com)
Key to their research, Dr. Marasco and his colleagues discovered and described the atomic structure of an obscure but genetically stable region of the influenza virus to which their monoclonal antibodies bind. (science20.com)
They scanned tens of billions of monoclonal antibodies produced in bacterial viruses, or bacteriophages, and found 10 antibodies active against the four major strains of H5N1 avian influenza viruses. (science20.com)
Encouraged by these findings, they collaborated with Ruben O. Donis, Ph.D., of the CDC Influenza Division, and found that three of these monoclonal antibodies had broader neutralization capabilities when tested in cell cultures and in mice against representative strains of other known influenza A viruses. (science20.com)
Their monoclonal antibodies neutralized all testable viruses containing the 10 Group 1 HAs-which include the seasonal H1 viruses, the H1 virus that caused the 1918 pandemic and the highly pathogenic avian H5 subtypes-but none of the viruses containing the six Group 2 HAs. (science20.com)
Simultaneously, Dr. Marasco's group teamed up with Robert C. Liddington, Ph.D., professor and chair of the Infectious and Inflammatory Disease Center at Burnham, to determine the atomic structure of one of their monoclonal antibodies bound to the H5N1 HA. (science20.com)
In this study, we describe a human monoclonal antibody (PN-SIA49) that recognizes a highly conserved epitope located on the stem region of the HA and able to neutralize a broad spectrum of influenza viruses belonging to different subtypes (H1, H2 and H5). (distantreader.org)

Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guerin mutants that secrete listeriolysin. (hum-molgen.org)
HFB30132A is an anti-SARS-CoV-2 recombinant antibody engineered with specific sequences identified from the B cells of a COVID-19 convalescent patient. (hifibio.com)
His research in the fields of Recombinant virus overlaps with other disciplines such as Sf9. (research.com)

Mutation in plasmids that contained R292 wild-type virus or 292K mu- tant virus, respectively. (cdc.gov)
6 ). The assay detected the 292K mutant in the mixture rus with this mutation to monitor its circulation and trans- at a proportion of 2% of the 104 copies/reaction, and cor- mission and guide antiviral treatment. (cdc.gov)
Majority of both experimental 11 - 14 and computational 15 - 17 efforts for inhibitor design are focused on the receptor binding domain (RBD) despite the fact that this region is highly mutation prone. (biorxiv.org)
The I304N mutation in a fragile X syndrome patient impaired the binding of a unique peptide motif in NSP3 to the two main KH domains of FMRPs. (worldmedicinefoundation.com)
The various areas that he examines in his Virus study include Immunoprecipitation, Lysis and Point mutation. (research.com)
Le génotype D du virus de l'hépatite B était le seul détectable chez ces patients, et aucune mutation du déterminant « a » n'a été observée dans les isolats. (who.int)

This inhibition reflects the binding of Munc18-1 to a closed conformation of the target-SNARE syntaxin1. (jneurosci.org)

Since little is known about how SV-40 activates within hosts nor the potency or transmission pathway of the virus and its different strains, its role in the current cancer epidemic is still being discovered. (sunridgemedical.com)
5 It suggests a strong possibility of generating new strains of this coronavirus and its family with higher virulence and more complicated epidemiology with a D614G mutant as a notable example. (biorxiv.org)
The antibody also efficiently neutralizes a panel of mutated SARS-CoV-2 viral strains including the well described mutant D614G. (hifibio.com)

2020. Cancer antigen discovery is enabled by RNA-sequencing of highly purified malignant and non-malignant cells . (cardiff.ac.uk)
The most important protective antigen on the surface of influenza virus is HA, whose structure can be divided in two distinct regions: the globular head, responsible for the binding to the sialic acid, and the stem region that contains the fusion peptide and the membrane anchor domain. (distantreader.org)
His Capsid study combines topics from a wide range of disciplines, such as Plasma protein binding, Immune system, Antigen, Peptide sequence and Peptide. (research.com)

Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)

A subset of mutated envelopes was also studied in direct CD4 or CCR5 binding assays and in envelope-mediated fusion reactions. (unige.ch)

When they surveyed more than 6,000 available HA genetic sequences of the 16 HA subtypes, they found the pockets to be very similar within each Group but to be significantly different between the two Groups. (science20.com)
Based on its molecular genetic characteristics, the virus has been classified into the Hepacivirus genus of the family Flaviviridae. (monash.edu)
However, their high genetic variability allows the virus to evade the host immune response and the potential protection offered by seasonal vaccines. (distantreader.org)
Through genetic analyses, we found that Cas12a mismatch cheapest vaniqa tolerance varies depending on the ability of bacteria expressing crRNAs with mismatches (MM crRNA) at different stages of development to ensure population of mutant emergence causing the largest reduction of target sequences for gene J or L were used for sequencing of mutant. (radeps.com)
Influenza detection was genetic material of influenza viruses. (who.int)
Investigation of genetic defects in severe combined immunodeficiency patients from Turkey by targeted sequencing. (medscape.com)
Sequencing and other techniques may reveal the actual genetic defects in these patients. (medscape.com)

In comparison, actin is more abundant in the Supporting information figures or used for the rapid emergence of PAM-distal mutants. (radeps.com)

We found that 5% of a database of 465 million human antibody sequences has the same residues at T2 and T6 positions in HCDR3 and Tyr in HCDR1 that could potentially form this Asp-Tyr interaction, and that this interaction might contribute to a non-canonical bulged torso conformation. (elsevierpure.com)

By using sequence alignment of herpesviruses homologs, we identified conserved amino acids in the putative pUL105 ATP binding site and in the putative pUL70 zinc finger pattern. (frontiersin.org)
This is so despite the fact that human TRIM5a is 87% identical in amino acid sequence to the rhesus form. (natap.org)
They altered blocks of 10 amino acids to Ala in the inherently disordered areas of NSP3 1-181 to find the FMRP binding site. (worldmedicinefoundation.com)
The team mapped the binding site in UBAP2L to FMRPs and constructed a peptide array encompassing the UBAP2L area with 20mer peptides moved by two amino acids at a time to investigate whether the interactome data indicated that UBAP2L and NSP3 compete for binding to a comparable interface on FMRPs. (worldmedicinefoundation.com)
The primary amino acid sequence of the two isoforms is identical. (bvsalud.org)

1 , 2 Largely expressed on the surface of SARS-CoV-2, the spike (S) protein plays a crucial role in binding to the host angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD) and facilitating viral entry, 3 , 4 which is therefore considered as one of the most preferred targets against SARS-CoV-2. (biorxiv.org)
E2 has a receptor binding domain (E2661RBD) that conatins three variable regions, hypervariable regions 1 (HVR1), HVR2 and the intergenotypic variable region (igVR). (monash.edu)

Of the two methods, real-time RT-PCR has many about 85-90% of all influenza viruses during August advantages over conventional RT-PCR. (who.int)

SARS-CoV-2 has consistently mutated over the course of the pandemic, resulting in variants that are different from the original SARS-CoV-2 virus. (cdc.gov)

However, to their surprise, the researchers found that the spike protein of the new coronavirus actually bound far better than computer predictions, likely because of natural selection on ACE2 that enabled the virus to take advantage of a previously unidentified alternate binding site. (nih.gov)

When present together, these two determinants affect the early steps of the virus cycle including cell binding and the receptor-mediated conformational changes believed to be necessary for viral penetration and uncoating. (pasteur.fr)
BPC1 induces conformational changes by cooperative binding to purine-rich elements present in the STK regulatory sequence. (riken.jp)

No matter where you go online these days, there's bound to be discussion of coronavirus disease 2019 (COVID-19). (nih.gov)
One of these adaptations provides special ability of this coronavirus to bind to a specific protein on human cells called angiotensin converting enzyme (ACE2). (nih.gov)
Existing computer models predicted that the new coronavirus would not bind to ACE2 as well as the SARS virus. (nih.gov)
On average, people infected with the delta variant had about 1,000 times more copies of the virus in their respiratory tracts than those infected with the original strain of the coronavirus, the study reported. (nakedcapitalism.com)

In seroconverters from the CAPRISA 004 tenofovir gel trial, we previously reported significantly higher detection and titres of HIV-specific binding antibodies in the plasma and genital tract (GT) that distinguished the tenofovir from the placebo arm. (edctpalumninetwork.org)

However, the region that binds ACE2 resembles a novel virus found in pangolins, a strange-looking animal sometimes called a scaly anteater. (nih.gov)

This potent oncogenic virus, with strong links to certain types of cancers like brain cancer, bone cancer, mesothelioma, and Lymphoma, shows up in the DNA of these cancerous cells. (sunridgemedical.com)
With the suppressed anti-tumor gene these new mutant cells are able to reproduce uncontrollably, forming tumors. (sunridgemedical.com)
2018. The novel oncolytic adenoviral mutant Ad5-3Δ-A20T retargeted to αvβ6 integrins efficiently eliminates pancreatic cancer cells . (cardiff.ac.uk)
When Vif is present in sufficient quantity in virus-producing cells, APOBEC3G is no longer incorporated into virus particles. (natap.org)
Stress granules, large protein-ribonucleic acid (RNA) assemblies, are crucial for viruses to replicate in host cells. (worldmedicinefoundation.com)
However, the exact molecular basis of Fragile X syndrome remains unknown, and the interactions between viruses and host cells are crucial for viral replication. (worldmedicinefoundation.com)
Furthermore, YFP-tagged fragments of NSP3 were produced in Henrietta Lacks (HeLa) cells, followed by immunological purification and Western blot analysis to assess fragile X mental retardation protein 1 (FXR1) binding. (worldmedicinefoundation.com)
MagPure nucleic acid extraction kit is suitable for use with blood commonly treated with anticoagulants such as citrate, heparin or EDTA, biological fluids, Paraffin-enbedded tissue, animal or plant tissues, cultured cells, bacterial cells carrying plasmid and virus sample. (bigfishgene.com)
Interaction with human PPIA/CYPA protects the virus from restriction by human TRIM5-alpha and from an unknown antiviral activity in human cells. (proteopedia.org)

Through direct competition with a peptide motif in UBAP2L, NSP3 binding to FMRPs inhibited their interaction with the stress granule component UBAP2L, preventing FMRP integration into stress granules. (worldmedicinefoundation.com)

In this study, by characterization of the dominant maize mutant Barren inflorescence3 ( Bif3 ) we identified a tandem-duplicated copy of ZmWUS1 whose expression is dramatically enhanced by the insertion of a short stretch of chimeric proximal promoter sequence. (nature.com)

To study the contribution of the SARS-CoV-2 non-structural protein 3-FMRP interactions in the in vivo settings, the researchers infected hamsters with the SARS-CoV-2 wild-type strain and non-structural protein 3 mutant, observing weight reduction and illness over seven days. (worldmedicinefoundation.com)

To test the performance of the assay when 292K mu- don/2013_10_24a/en/index.html). (cdc.gov)
Studies have shown that the NA R292K muta- of the mixture a ratio of 50:50 was used as the assay-specif- tion can cause a high level of resistance to oseltamivir in ic normalization value in determination of the percentage influenza A(H7N9) virus ( 4,5 ). (cdc.gov)
To validate the assay with clinical samples, we tested ate NA 292K mutant virus from R292 wild-type virus in 11 paired nasopharyngeal swab specimens and sputum clinical samples. (cdc.gov)
Moreover, several association-incompetent mutants failed to complement the replication of a pol null mutant in a transient transfection assay, confirming that the Pol-UL42 interaction is necessary for virus replication in vivo and therefore a valid target for directed drug design. (ed.ac.uk)

Promising new inhibitors that target the viral helicase-primase complex have been reported to block replication of herpes simplex and varicella-zoster viruses, but they have no activity against human cytomegalovirus (HCMV), another herpesvirus. (frontiersin.org)
Oddly, a Vif mutant that could not be phosphorylated at Ser 144 was defective in HIV replication but not in APOBEC3G degradation. (natap.org)

Conversely, expressing the Munc18-2 isoform, which also displays binding to closed syntaxin1, rescued vesicle docking identical with Munc18-1 but impaired more downstream vesicle priming steps. (jneurosci.org)

The controversy would be solved if binding to closed syntaxin1 were shown to be stimulatory for vesicle fusion and/or additional essential interactions were identified between Munc18-1 and the fusion machinery. (jneurosci.org)

Thus, sensitive molecular of 292K mutant in mixed population as described by Liu et techniques are needed for rapid detection of influenza vi- al. (cdc.gov)
High purity DNA for Gene chip detection and high-throughput sequencing. (bigfishgene.com)
With nearly 20 years' experience in hardware and software developing, reagent application and products manufacturing of gene detection instruments and reagents, Bigfish team concentrates on molecular diagnosis POCT and mid-to-high level gene detection technology (Digital PCR, Nanopore sequencing, etc. (bigfishgene.com)

The key regulation of these motifs might be phosphorylation of a portion of MA molecules on the C-terminal tyrosine at the time of virus maturation, by virion-associated cellular tyrosine kinase. (proteopedia.org)

The Env protein of Human Immunodeficiency Virus (HIV) also has a trimeric structure of heterodimers. (wikipedia.org)
Then, as a result of gradual evolutionary changes over years or perhaps decades, the virus eventually gained the ability to spread from human-to-human and cause serious, often life-threatening disease. (nih.gov)
But none did so with as much devastation to human life as was done by the SARS-CoV-2-WIV virus. (substack.com)
Sequence requirements for nucleolar localization of human T cell leukemia virus type I pX protein, which regulates viral RNA processing. (wikidata.org)
Scientists have found a molecule that interferes with the formation of infectious Human immunodeficiency virus (HIV) particles. (hum-molgen.org)
We studied the evolution of human immunodeficiency virus type 1 (HIV-1) envelope function during the process of coreceptor switching from CCR5 to CXCR4. (unige.ch)
Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction. (research.com)
Disrupting either the perfect or MM15 crRNA (Fig 3F), suggesting that recruitment of the target regions at the binding of Orytolagus cuniculus actin and human profilin (PDB: 2PBD) (56) were utilised as a loading control. (radeps.com)
Previous animal and human studies demonstrated reduced HIV-specific antibody binding avidity post-HIV seroconversion with PrEP, which in turn may affect antibody functionality. (edctpalumninetwork.org)
3d1z is a 2 chain structure with sequence from Human immunodeficiency virus type 1 (BH5 ISOLATE) . (proteopedia.org)

Engineered resonance energy transfer (RET) between a mTurquoise2 fluorescent protein and FMN-bound As LOV2 increases switch sensitivity. (nature.com)

They describe the specific aims of the project, which include creating mutant viruses SARS (and MERS viruses) "to better predict the capacity of our CoVs [coronaviruses] to infect people. (substack.com)
Introduction The Omicron variant of the SARS-CoV-2 virus is described as more contagious than previous variants. (researchgate.net)
The etiological agent, SARS-CoV-2, is a member of the Coronaviridae family including SARS-CoV-1 (2002-2004) and MERS-CoV (since 2012), with the sequence identity of 79.6% and 50%, respectively. (biorxiv.org)
Scientists use multiple classification systems to describe and communicate similarities and differences between SARS-CoV-2 viruses. (cdc.gov)
There are multiple ways in which SARS-CoV-2 viruses are classified. (cdc.gov)
These classification methods enable scientists to communicate similarities and differences between SARS-CoV-2 viruses. (cdc.gov)

The results characterized the motif in NSP3 binding to FMRPs and showed that it was conserved across the Sarbecovirus family. (worldmedicinefoundation.com)

It is believed that the intracellular transport of the nascent protein depends, to some extent, on the oligomerization of Env precursors, which allows hydrophobic sequences to be buried inside the protein structure. (wikipedia.org)

Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. (nature.com)

A lineage is a group of closely related viruses with a common ancestor. (cdc.gov)

Their detailed picture shows one arm of the antibody inserted into a genetically stable pocket in the neck of the HA protein, an interaction that blocks the shape change required for membrane fusion and virus entry into the cell. (science20.com)
His Intracellular study deals with Cell intersecting with Membrane glycoproteins, Receptor, Chinese hamster ovary cell, Virus maturation and Cleavage. (research.com)
Matrix protein p17 has two main functions: in infected cell, it targets Gag and Gag-pol polyproteins to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus. (proteopedia.org)
The drug binds to sterols (eg, ergosterol) in the fungal cell membrane, causing leakage of intracellular components and fungal cell death. (medscape.com)

Anatomy14

Organisms71

Diseases11

Chemicals and Drugs60

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes76

Information Science4

Health Care1

Proteins19

Mutations16

Genes6

Escape mutants1

Antibody binds2

Glycoprotein5

Vitro6

Herpes Simpl2

Polymerase5

Nucleotide sequence2

Infection10

Host cell receptors2

Endogenous4

H1N18

Molecular Biology3

CDNA1

Genomic4

Gene Sequences1

Deletion4

Transcription7

Hepatitis C vir1

Genome4

Monoclonal7

Recombinant3

Mutation6

Inhibition1

Strains3

Antigen3

Regulate1

Assays1

Genetic7

Emergence1

Residues1

Amino5

Receptor-bindin2

Influenza viruses1

Pandemic1

Spike protein1

Conformational changes2

Coronavirus4

Antibodies1

ACE21

Cells9

Peptide1

Chimeric1

Strain1

Assay4

Replication2

Isoform1

Interactions1

Detection3

Molecules1

Human10

Fluorescent1

SARS6

Motif1

Protein structure1

Reveals1

Closely related viruses1

Membrane4