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  • induce
  • Good proportionality was observed between the strength of an agonist (coupling efficiency) and its capacity at high occupancy to induce the rapid phase of desensitization, internalization, and phosphorylation. (aspetjournals.org)
  • activation
  • Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. (merckmillipore.com)
  • Thus the protein phosphorylation-driven conformational change of p47 phox enables its PX domain to bind to phosphoinositides, the interaction of which plays a crucial role in recruitment of p47 phox from the cytoplasm to membranes and subsequent activation of the phagocyte oxidase. (pnas.org)
  • The activation of the oxidase requires the proteins p47 phox and p67 phox and the small GTPase Rac, which exist in the cytoplasm of resting phagocytes and translocate after cell stimulation to membranes to interact with the cytochrome, leading to superoxide production ( 6 , 15 - 19 ). (pnas.org)
  • Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. (abcam.com)
  • Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. (abcam.com)
  • specific
  • A variety of protein modules such as pleckstrin homology and FYVE domains recognize specific phosphoinositides (phosphorylated forms of PtdIns) to recruit proteins to appropriate cell membranes ( 1 , 2 ). (pnas.org)
  • Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. (abcam.com)
  • thus
  • Since many diseases are caused by, or correlated with, changes in protein expression patterns, comparing protein expression patterns between normal and disease conditions can reveal proteins whose altered expression is critical in causing the disease and can thus identify appropriate therapeutic targets. (google.com)
  • New techniques for evaluating expression of several proteins simultaneously are thus desirable. (google.com)
  • 1997
  • Johnson and Jaramillo 1997), and treatment with chrysotile induced the elevation of p53 protein level in rat pleural mesothelial cells (Levresse et al. (thefreelibrary.com)
  • activity
  • Moreover, increased MAPK activity seems to play a role in the phosphorylation of IRS-1 on serine residue in human muscle cells. (diabetesjournals.org)
  • Although the isolated phox homology (PX) domain of p47 phox can interact directly with phosphoinositides, the lipid-binding activity of this protein is normally suppressed by intramolecular interaction of the PX domain with the C-terminal Src homology 3 (SH3) domain, and hence the wild-type full-length p47 phox is incapable of binding to the lipids. (pnas.org)
  • However, it is presently unknown how the lipid-binding activity of PX domains is regulated. (pnas.org)
  • cells
  • In cells exposed to two different types of asbestos, chrysotile (~1-6% iron content) and crocidolite (~27% iron content) fibers, at the doses of 1, 5, and 10 [micro]g/[cm.sup.for 24 hr, the levels of p53 phosphorylated at Ser15 and p53 protein were correlated with the dose. (thefreelibrary.com)
  • Among serines in p53 protein immunoprecipitated from A549 cells treated with chrysotile, only Ser15 was markedly phosphorylated. (thefreelibrary.com)
  • show
  • Here we show that protein phosphorylation of p47 phox , a cytoplasmic activator of the microbicidal phagocyte oxidase (phox), elicits interaction of p47 phox with phosphoinositides. (pnas.org)
  • interaction
  • Protein-phosphoinositide interaction participates in targeting proteins to membranes where they function correctly and is often modulated by phosphorylation of lipids. (pnas.org)
  • The W263R substitution in this SH3 domain, abrogating the interaction with the PX domain, leads to a binding of p47 phox to phosphoinositides. (pnas.org)
  • Complex
  • In addition, some proteins are involved in protecting telomere extremities and make up a complex called the telosome ( 5 ) or shelterin ( 6 ). (aacrjournals.org)
  • This complex is composed of six proteins including TRF1 ( 7 , 8 ), TRF2 ( 9 ), POT1 ( 10 ), TIN2 ( 11 ), TPP1 ( 12 ), and Rap1 ( 13 ). (aacrjournals.org)
  • function
  • In addition to telomere attrition, more recent experiments suggest that telomere deprotection can also be induced by the loss of function of double stranded telomere-binding proteins at the telomere. (aacrjournals.org)
  • Indeed it has been uncovered recently that PX domains function as a phosphoinositide-binding module ( 9 - 14 ). (pnas.org)
  • expression
  • Similarly, protein expression profiles can be used in clinical diagnosis, prognosis and monitoring therapy. (google.com)
  • However, lack of large-scale protein screening methods is a major obstacle in profiling protein expression patterns. (google.com)
  • domain
  • The ph agocyte ox idase (phox) homology (PX) domain ( 5 ), also known as the phox and Bem1p 2 (PB2) domain ( 6 , 7 ), occurs in the phox proteins p47 phox and p40 phox in mammals, the polarity establishment protein Bem1p in budding yeast, and a variety of eukaryotic proteins involved in membrane trafficking. (pnas.org)
  • We have determined the NMR structure of the PX domain of p47 phox and demonstrated that it interacts with the C-terminal Src homology 3 (SH3) domain of this protein ( 8 ). (pnas.org)
  • response
  • When the subject learns the association between the CS and the US, the subject may display a response similar to the UR upon exposure to the CS alone. (biomedcentral.com)
  • Many DNA damage response proteins, including ATM, are thought to participate in telomere maintenance. (aacrjournals.org)
  • several
  • Several related methods for multiplexed detection of one or more posttranslational modifications of a plurality of proteins are provided. (google.com)
  • however, this method is limited by the need for a relatively large sample size for detection of several cellular proteins in a test sample. (google.com)
  • clinical
  • 2000). It has been reported that the frequency of p53 protein accumulation is increased in lung carcinomas of patients with clinical or histologic asbestos exposure (Nuorva et al. (thefreelibrary.com)
  • Analysis
  • This application claims priority to and benefit of U.S. Provisional Patent Application No. 60/448,750, filed Feb. 19, 2003, "Multiplex Analysis of Proteins" by Paul A. Luciw et al. (google.com)