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  • novel
  • To gain further insight into the molecular mechanisms of LAP2α functions in chromatin organization and cell cycle control, we aimed at identifying novel LAP2α interaction partners in a yeast two-hybrid screen. (biologists.org)
  • Here we report a novel 25 kDa protein termed LAP2α interactor-25 (LINT-25) that binds to the LAP2α-specific C-terminus. (biologists.org)
  • Ropp PA, Hu M, Olesky M, Nicholas RA (2002) Mutations in ponA , the gene encoding penicillin-binding protein 1, and a novel locus, penC , are required for high-level chromosomally mediated penicillin resistance in Neisseria gonorrhoeae . (springer.com)
  • 2000
  • Marrec-Fairley M, Piette A, Gallet X et al (2000) Differential functionalities of amphiphilic peptide segments of the cell-septation penicillin-binding protein 3 of Escherichia coli . (springer.com)
  • vivo
  • RBP45 and RBP33 are phosphoproteins, and RBP45 has been found to bind in vivo specifically to target mRNA containing cycling sequences. (asm.org)
  • These include a family of RNA binding proteins recently identified from Trypanosoma cruzi that shows highly restrictive binding interactions in vivo with specific mRNAs ( 9 ). (asm.org)
  • Normal cancer cells and Cdc42-deficient cancer cells have also been compared in vivo. (wikipedia.org)
  • interaction
  • Collectively, these results indicate that APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells, through the interaction with APP, which is fostered by phopshorylation of threonine 668. (duhnnae.com)
  • Frere JM, Nguyen-Disteche M, Coyette J, Joris B (1992) Mode of action: interaction with the penicillin binding proteins. (springer.com)
  • Selective interaction of JNK protein kinase isoforms with transcription factors. (nii.ac.jp)
  • distinct
  • A second distinct binding protein termed CSBP II has been purified from CSBPA null mutant cells, lacking both CSBPA and CSBPB proteins, and contains three major polypeptides with predicted molecular masses of 63, 44.5, and 33 kDa. (asm.org)
  • Spratt BG (1975) Distinct penicillin-binding proteins involved in the division, elongation, and shape of Escherichia coli . (springer.com)
  • Extracellular Wnts bind a variety of different receptors, and initiate signaling in several distinct pathways. (antibodies-online.com)
  • Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. (wikipedia.org)
  • yeast
  • It was originally identified in S. cerevisiae (yeast) as a mediator of cell division, and is now known to influence a variety of signaling events and cellular processes in a variety of organisms from yeast to mammals. (wikipedia.org)
  • CDT1 belongs to a family of replication proteins conserved from yeast to humans. (wikipedia.org)
  • motif
  • Only those with a suitable CaaX motif can bind ('C' is Cysteine, 'a' is an aliphatic amino acid, and 'X' is variable). (wikipedia.org)
  • sequence
  • Polypeptides of identical size were radiolabeled in UV cross-linking assays performed with purified CSBP II and 32 P-labeled RNA probes containing six copies of the cycling sequence. (asm.org)
  • Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. (wikipedia.org)
  • Its sequence domains include a P-loop containing nucleoside triphosphate hydrolase and a small GTP-binding protein domain. (wikipedia.org)
  • 1999
  • Zhao G, Meier TI, Kahl SD et al (1999) BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins. (springer.com)
  • Vollmer W, von Rechenberg M, Holtje JV (1999) Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli . (springer.com)
  • membrane
  • This process, called farnesylation (which is a type of prenylation), causes farnesylated proteins to become membrane-associated due to the hydrophobic nature of the farnesyl group. (wikipedia.org)
  • Most farnesylated proteins are involved in cellular signaling wherein membrane association is critical for function. (wikipedia.org)
  • There, it activates Bak, a pro-apoptotic protein bound to the mithochondria outer membrane (MOM). (wikipedia.org)
  • Invading cancer cells send out protrusions that reach down between neighboring endothelial cells to contact the underlying basement membrane. (wikipedia.org)
  • In the absence of Cdc42, cancer cells failed to spread out on the basement membrane, and Cdc42-deficient cells showed reduced adhesion to extracellular matrix-coated coverslips. (wikipedia.org)
  • Cdc42 therefore promotes the attachment of cancer cells to both endothelial cells and the underlying basement membrane during transendothelial migration. (wikipedia.org)
  • HeLa
  • Cdc42 expression was gradually increased showing significant difference and was significantly higher in HeLa cells than in regular cells. (wikipedia.org)
  • The migration ability of HeLa cells transfected with Cdc42 was higher than that of non-transfected cells. (wikipedia.org)
  • It was proposed that the overexpression of Cdc42 can promote filopodia formation in HeLa cells. (wikipedia.org)
  • structurally
  • These bacteria can produce β-lactamases that hydrolyze the antibiotic, alter their permeability by increasing efflux or decreasing influx (Gram-negative-specific), and structurally alter existing penicillin-binding proteins (PBPs) to lower the rate of acylation or obtain a completely new penicillin-binding protein with extremely low rates of acylation by most β-lactam antibiotics. (springer.com)
  • cytoplasm
  • In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. (wikipedia.org)
  • amino
  • The carboxyl-terminal amino acid (X) discriminates FTase's targets from those of the other prenyltransferases, allowing only six different amino acids to bind with any affinity. (wikipedia.org)
  • The full length human protein has 270 amino acids (31 kDa) with a centrally located RNA binding domain (RBD). (wikipedia.org)
  • The 75 amino acid RBD is well conserved across species, however the remainder of SLBP is highly divergent in most organisms and not homologous to any other protein in the eukaryotic genomes. (wikipedia.org)
  • nucleic
  • The present invention provides methods for characterizing a skin sample of a subject as belonging to an age range by analyzing nucleic acid or protein molecules obtained from the subject. (freepatentsonline.com)
  • 39. A kit for characterizing a skin sample from a subject comprising a skin sample collection device, one or more probes or primers that selectively bind to one or more nucleic acid molecules expressed from a young gene, and one or more probes or primers that selectively bind to one or more nucleic acid molecules expressed from an old gene. (freepatentsonline.com)
  • The gene product contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. (wikipedia.org)
  • cellular
  • They are the principal components in an interconnected intracellular "highway" by which all manner cellular cargo may be shuttled via a bustling network of molecular motor proteins. (antibodies-online.com)
  • Wnt signaling can also prompt morphological changes to cellular structure e.g., the non-canonical planar cell polarity pathway induces a kinase cascade that results in reorganization of actin, a core component of the cytoskeleton. (antibodies-online.com)
  • peptides
  • Secretase-mediated cleavage of amyloid precursor protein (APP) leads to the production of Alzheimer disease-related amyloid- (A) peptides. (biomasswars.com)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. (wikipedia.org)
  • inhibition
  • Over-expression of PPARδ also sensitised THP-1 cells to phorbol ester and correspondingly, inhibition of PPARδ by anti-sense RNA completely abolished this response. (biomedcentral.com)
  • Meanwhile, several other proteins interact directly with the oriC sequence to regulate initiation, usually by inhibition. (wikipedia.org)
  • A few other mechanisms in E. coli that variously regulate initiation are DDAH (datA-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the dnaA gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane. (wikipedia.org)
  • β-catenin acts by anchoring the actin cytoskeleton to the junctions, and may possibly aid in contact inhibition signaling within the cell. (wikipedia.org)
  • nucleus
  • In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. (wikipedia.org)
  • ASK1 will be transported to the nucleus when UV-irradiation is used to treat the cell. (wikipedia.org)
  • TTC39A has been tested for association to diseases like breast neoplasms and is expected to have molecular binding function and localizes in various compartments (extracellular space, membrane, nucleus). (wikipedia.org)
  • In cells with a nucleus, as in eukaryotes, the cell cycle is also divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. (wikipedia.org)
  • Interphase is a series of changes that takes place in a newly formed cell and its nucleus, before it becomes capable of division again. (wikipedia.org)
  • antibodies
  • To keep the quality and the affinity of the antibodies cycles of freezing and thawing should be avoided. (antibody-antibodies.com)
  • This technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John McCafferty, The Scripps Research Institute with Lerner and Barbas and the German Cancer Research Center with Breitling and Dübel for display of proteins such as antibodies for therapeutic protein engineering. (wikipedia.org)
  • selective
  • Evaluation of cytotoxicity in Human noncancer cells indicated that the compounds 3i and 3m were practically ineffective in quiescent peripheral blood lymphocytes, and may have a selective antiproliferative activity against cancer cells. (deepdyve.com)
  • Transcription
  • Most TPR-containing proteins are associated with multiprotein complexes, and there is extensive evidence indicating that TPR motifs are important to the functioning of chaperone, cell-cycle, transcription, and protein transport complexes. (wikipedia.org)
  • The transcription start site for TTC39B protein isoform 1 is located from base pairs 15,307,340 to 15,307,389 and has a length of 50 bp. (wikipedia.org)
  • preferentially
  • The rest eight DnaA boxes are low affinity sites that preferentially bind to DnaA-ATP. (wikipedia.org)
  • showed that HAP1 is localized in mitotic spindle of dividing striatal cells, and associated endosomes, microtubules and vesicles in the basal forebrain and striatial neurons - where HAP1B is preferentially expressed. (wikipedia.org)
  • membrane-bound
  • Zoospores of B. dendrobatidis, which are typically 3-5 µm in size, have an elongate-ovoidal body with a single, posterior flagellum (19-20 µm long), and possess a core area of ribosomes often with membrane-bound spheres of ribosomes within the main ribosomal mass. (wikipedia.org)
  • adhesion
  • Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell cycle. (wikipedia.org)
  • Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. (wikipedia.org)
  • Cell-cell adhesion complexes are required for simple epithelia in higher organisms to maintain structure, function and polarity. (wikipedia.org)
  • The primary mechanical role of catenins is connecting cadherins to actin filaments, specifically in these adhesion junctions of epithelial cells. (wikipedia.org)
  • Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between vascular endothelial cells. (wikipedia.org)
  • Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. (wikipedia.org)
  • molecules
  • As these molecules are vital for life, metabolic reactions either focus on making these molecules during the construction of cells and tissues, or by breaking them down and using them as a source of energy, by their digestion. (wikipedia.org)
  • The BCR-ABL protein can be inhibited by various small molecules. (wikipedia.org)
  • organism
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. (wikipedia.org)
  • subcellular localization
  • The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. (wikipedia.org)
  • Gutekunst and colleagues used immunogold labeling to identify subcellular localization of both HAP1 and muHtt, and identified a close similarity of the distribution of the two proteins. (wikipedia.org)
  • interactions
  • Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. (wikipedia.org)
  • The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. (wikipedia.org)
  • The TPR domains are found in many proteins that facilitate specific interactions with a partner protein. (wikipedia.org)
  • substrate
  • The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, open the gate of 20S core particle, and guide the substate into the proteolytic chamber. (wikipedia.org)
  • To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special degradative tag, the ubiquitinylation. (wikipedia.org)
  • Molecular
  • Ferroptosis is a kind of regulated cell loss of life driven by oxidative damage promoting lipid peroxidation, although detailed molecular regulators are largely unknown. (biomasswars.com)
  • Molecular docking studies indicated that compounds 3i and 3m occupy the colchicine binding site of tubulin. (deepdyve.com)
  • Molecular Cell. (wikipedia.org)
  • cardiac
  • Cardiac arrest Cytokine release syndrome Tumor lysis syndrome, causing acute renal failure Infections Hepatitis B reactivation Other viral infections Progressive multifocal leukoencephalopathy (PML) Immune toxicity, with depletion of B cells in 70% to 80% of lymphoma patients Pulmonary toxicity Bowel obstruction and perforation Two patients with systemic lupus erythematosus died of progressive multifocal leukoencephalopathy (PML) after being treated with rituximab. (wikipedia.org)
  • motifs
  • TPR motifs that are arranged one in front of another create a right-handed helical structure with an amphipathic channel which could possibly accommodate the complementary region of a target protein. (wikipedia.org)
  • enzymes
  • Many proteins are enzymes that catalyze the chemical reactions in metabolism. (wikipedia.org)
  • B. dendrobatidis also contains a variety of proteolytic enzymes and esterases that help it digest amphibian cells and use amphibian skin as a nutrient source. (wikipedia.org)
  • mitotic
  • The word "post-mitotic" is sometimes used to refer to both quiescent and senescent cells. (wikipedia.org)
  • Some cells enter the G0 phase semi-permanently and are considered post-mitotic, e.g., some liver, kidney, and stomach cells. (wikipedia.org)
  • Escherichia
  • Since protein expression in Escherichia coli represents the most facile approach (Mahalik et al. (springeropen.com)
  • The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into Escherichia coli (E. coli) bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
  • For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. (wikipedia.org)
  • complexes
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • α-catenin participates in the formation and stabilization of adherens junctions by binding to β-catenin-cadherin complexes in the cell. (wikipedia.org)
  • coli cells
  • The yield and productivity of gamma-aminobutyric acid might be significantly improved if the overexpressed GadB in E. coli cells can be excreted outside, where it can directly transforms monosodium glutamate to gamma-aminobutyric acid. (springeropen.com)
  • If a "phagemid" vector is used (a simplified display construct vector) phage particles will not be released from the E. coli cells until they are infected with helper phage, which enables packaging of the phage DNA and assembly of the mature virions with the relevant protein fragment as part of their outer coat on either the minor (pIII) or major (pVIII) coat protein. (wikipedia.org)
  • mRNA
  • The protein encoded by this gene is involved in mRNA splicing and may play a role in site selection in alternative splicing. (wikipedia.org)
  • progenitor
  • Background Human being fetal midbrain-derived neural progenitor cells (NPCs) might deliver a cells source for medication testing and regenerative cell therapy to take care of Parkinsons disease. (biomasswars.com)