Binding protein actin binding protein most actin binding proteins bind. Medical search. Frequent questions

The actin cytoskeleton is a complex structure that performs a wide range of cellular functions. (edu.au)
In particular, 1) the structure of actin was resolved from crystals in the absence of cocrystallized actin binding proteins (ABPs), 2) the prokaryotic ancestral gene of actin was crystallized and its function as a bacterial cytoskeleton was revealed, and 3) the structure of the Arp2/3 complex was described for the first time. (edu.au)
The insulin receptor substrate p53 (IRSp53), an Src homology 3 (SH3) adapter protein and regulator of the actin cytoskeleton, was identified as an espin-binding protein in yeast two-hybrid screens. (northwestern.edu)
Thus, the PC espins exhibit the properties of modular actin-bundling proteins with the potential to influence the organization and dynamics of the actin cytoskeleton in PC dendritic spines and to participate in multiprotein complexes involving SH3 domain-containing proteins, such as IRSp53. (northwestern.edu)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
The actin cytoskeleton is essential in eukaryotes, not least in the plant kingdom where it plays key roles in cell expansion, cell division, environmental responses and pathogen defence. (biorxiv.org)
In addition to these results, our algorithm is designed to be easily adaptable to other tissues, mutants and plants, and so will be a valuable asset for the study and future biological engineering of the actin cytoskeleton in globally-important crops. (biorxiv.org)
In addition, this problem is by far the most acute for actin, the narrowest element of the cytoskeleton, which is ubiquitous throughout eukaryotic cells. (biorxiv.org)
Formed from polymerisation of discrete protein sub-units, the cytoskeleton connects to various organelles (including the nucleus) and the plasma membrane. (biorxiv.org)
The cytoskeleton is typically divided into three distinct components: microtubules, intermediate filaments and actin[ 4 , 5 ]. (biorxiv.org)
Finally, actin filaments, also known as microfilaments, are the narrowest components of the cytoskeleton and are constructed from globular actin sub-units (G-actin) that assemble to form a helical structure 5-7nm in diameter[ 12 ]. (biorxiv.org)
This thesis is focused primarily on the effect that LTD4 has on reorganisation of the actin cytoskeleton and on cell-cell and cell-matrix adhesion properties. (lu.se)
We found that LTD4 caused dramatic changes in the actin cytoskeleton in intestinal epithelial cells, and an important factor in this context was the impact of this leukotriene on the actin-binding protein vinculin, which included inducing translocation of vinculin from a cell-cell to a cell-matrix complex. (lu.se)

The PC espins efficiently bound and bundled actin filaments in vitro, and these activities were not inhibited by Ca 2+ . (northwestern.edu)
When expressed in transfected neuronal cell lines, the PC espins colocalized with actin filaments and elicited the formation of coarse cytoplasmic actin bundles. (northwestern.edu)
Growth-cone collapse induced by ephrin-As, a family of repulsive axon guidance molecules, is impaired upon R62D expression, resulting in perseverance of ring-shaped F-actin filaments. (jneurosci.org)
Unlike the globular units of microtubules and actin filaments, intermediate filaments are themselves constructed from filamentous sub-units, and confer strength as well as stress resistance to the cell[ 10 ]. (biorxiv.org)
Actin microfilaments are present as both individual filaments and bundled into thicker filaments, and play a key role in plant cell growth and internal transport[ 14 ]. (biorxiv.org)
Cell-cell and cell-matrix complexes of epithelial cells are interconnected through cytoskeletal filaments and proteins, and they influence the activities and outcome of various cellular processes. (lu.se)

Actin-binding proteins (also known as ABPs) are proteins that bind to actin. (wikipedia.org)

Moreover, R62D NLS decreases neuronal motility similar to the cytoplasmic R62D actin mutant although R62D NLS has no access to cytoplasmic actin dynamics. (jneurosci.org)
Thus, herein we provide first evidence that neuronal motility not only depends on cytoplasmic actin dynamics but also on the availability of actin to modulate nuclear functions such as gene transcription. (jneurosci.org)

Many of these are likely to help us understand and distinguish between the structural models of actin microfilaments. (edu.au)

These proteins are novel isoforms of the actin-bundling protein espin that arise through the use of a unique site for transcriptional initiation and differential splicing. (northwestern.edu)
SRF controls gene transcription of various actin isoforms (e.g. (jneurosci.org)
Here we analyzed neuronal actin signaling by a novel approach using actin mutant isoforms known to favor or preclude actin polymerization. (jneurosci.org)
In line with a more complex picture, multiple isoforms of the protein likely exist and non-centrosomal sites of localisation have been reported. (springer.com)

This may mean ability to bind actin monomers, or polymers, or both. (wikipedia.org)
In 2001, significant advances were made to our understanding of the structure and function of actin monomers. (edu.au)

Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
Neuronal motility relies on actin treadmilling. (jneurosci.org)
We present a hitherto unappreciated cross talk of actin signaling with gene expression governing neuronal motility. (jneurosci.org)
We show that neuronal motility evoked by these actin mutants requires SRF activity. (jneurosci.org)
Conversely, actin signaling regulates neuronal SRF-mediated gene expression. (jneurosci.org)
In addition to regulation of cytoplasmic cytoskeletal dynamics, a little appreciated property of actin signaling is modulation of gene expression so far only reported for non-neuronal cells. (jneurosci.org)

Many cellular processes including cell division and cell migration require coordination between the actin and microtubule (MT) cytoskeletons. (umn.edu)
The Abl-related gene (Arg) requires its F-actin-microtubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion. (nih.gov)

Many actin-binding proteins, including α-actinin, β-spectrin, dystrophin, utrophin and fimbrin, do this through the actin-binding calponin homology domain. (wikipedia.org)
Here, we designed a set of homology-directed repair donor templates that enable efficient tagging of endogenous proteins with affinity tags by transient transfection and selection of genome-edited cells in various human cell lines. (bvsalud.org)

The actin mutants G15S and S14C favor F-actin assembly, yet differ with regard to their ABP interaction. (jneurosci.org)
Employing these actin mutants, we uncovered a function for actin signaling in growth-cone motility modulated by ephrin-A guidance cues. (jneurosci.org)
Moreover these actin mutants affect neurite elongation, an actin function which by pharmacological actin interference was ambiguous. (jneurosci.org)
Using this algorithm, we then studied a number of cases in Arabidopsis thaliana , including several different tissues, a variety of actin-affected mutants, and cells responding to powdery mildew. (biorxiv.org)

The regulation of vertebrate striated muscle contraction involves a number of different molecules, including the thin-filament accessory proteins tropomyosin and troponin that provide Ca(2+)-dependent regulation by controlling access to myosin binding sites on actin. (egelmanlab.org)
Cardiac myosin binding protein C (cMyBP-C) appears to modulate this Ca(2+)-dependent regulation and has attracted increasing interest due to links with inherited cardiac diseases. (egelmanlab.org)
These observations help to explain many of the seemingly contradictory results obtained with cMyBP-C and show how cMyBP-C can provide an additional layer of regulation to actin-myosin interactions. (egelmanlab.org)
Regulation of MT dynamics via direct binding of an Abl family kinase. (nih.gov)
Further, there are signaling pathways that participate in regulation, such as Wnt channel, mitogen-activated protein kinase (MAPK) channel, bone morphogenetic protein (BMPs) channel, peroxisome proliferator activated-receptors (PPARs) channel, and Notch channel. (frontiersin.org)

It thus appears that the ability to bind to at least two distinctly different positions on F-actin, as observed for tropomyosin, may be more common than previously considered for other actin binding proteins. (egelmanlab.org)

We identified a group of actin-binding-bundling proteins that are expressed in cerebellar Purkinje cells (PCs) but are not detected in other neurons of the CNS. (northwestern.edu)
Overexpressing these mutant actins in mouse hippocampal neurons not only modulated growth-cone function but also neurite elongation, which was ambiguous by traditional pharmacological interference. (jneurosci.org)

Arg/Abl2 modulates the affinity and stoichiometry of binding of cortactin to F-actin. (nih.gov)
In addition to regulating cytoskeletal dynamics in the cytoplasm, actin modulates nuclear gene expression. (jneurosci.org)

Increased adhesion of the cancer cells depended on activation of cyclooxygenase-2, an enzyme that is involved in progression of colon cancers, whereas adhesion of the intestinal epithelial cells was augmented by LTD4-induced translocation of protein kinase C to areas where integrins bind to matrix proteins (focal adhesions). (lu.se)

The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. (egelmanlab.org)
One position aligns well with the previously reported binding site that clashes with the binding of myosin to actin, but would force tropomyosin into an "on" position that exposes myosin binding sites along the filament. (egelmanlab.org)
The second position identified here would not interfere with either myosin binding or tropomyosin positioning. (egelmanlab.org)
In combination with myosin motors, actin aids in transport by providing the roads and pathways for cellular cargo[ 13 ]. (biorxiv.org)

and transmembrane receptor protein tyrosine kinase signaling pathway. (nih.gov)
RANK binds to transmembrane protein nuclear factor-κB receptor activating factor ligand (RANKL) to activate RANK/RANKL, TRAF6/RANKL/MAPKs, TRAF6/ RANKL/NF-κB, Wnt/RANKL/RANKL, and JAK2/STAT3/RANKL. (frontiersin.org)

We show that the MT binding protein EB1 (end-binding protein 1), a key regulator of MT dynamics, can bind directly to filamentous actin (F-actin) F-actin. (umn.edu)

G15S actin enhanced neurite outgrowth and filopodia number. (jneurosci.org)

These observations suggest that EB1:F-actin interactions may negatively regulate EB1:MT interactions, and we speculate that this interaction may assist cells in differentially regulating MT stability in the actin-rich cortex as opposed to the cell interior. (umn.edu)

We determined that the EB1:F-actin interaction is salt sensitive and weak under physiological salt concentrations but might be relevant in contexts where the local concentration of actin is high. (umn.edu)

and protein tyrosine kinase activity. (nih.gov)

Using electron microscopy and three-dimensional reconstruction, we show that C0 and C1 can each bind to the same two distinctly different positions on F-actin. (egelmanlab.org)
Part of the problem lies in the difficulty of extracting high-quality, three-dimensional, quantitative measures of actin network features from microscopy data. (biorxiv.org)
Combined with technological advancements in single-particle cryogenic electron microscopy, this strategy allows efficient structural studies of endogenous proteins captured in their native cellular environment and during different cellular processes. (bvsalud.org)

Our results indicate that the active factor is either a high molecular weight protein or protein complex and is not the respiratory protein hemocyanin. (nature.com)

Using bioinformatics and mutagenesis, we found that the EB1:F-actin binding site partially overlaps the well-characterized EB1:MT binding interface. (umn.edu)

Gsn ) and is the archetypical transcription factor to study actin interplay with transcription. (jneurosci.org)
However, mechanistic details are lacking, and recent studies have implicated ALMS1 in several processes including endosomal trafficking, actin organisation, maintenance of centrosome cohesion and transcription. (springer.com)

M-CSF binds to the M-CSF receptor, which is promote the proliferation and differentiation of OC and the continuous expression of RANK. (frontiersin.org)
The CysLT1 receptor that specifically serves as receptor for leukotriene D4 (LTD4) has been identified as a G-protein coupled receptor. (lu.se)

They were chosen because 1) they represent a family of related proteins, 2) they are widely distributed in nature, 3) an atomic structure (or at least a plausible model) is available for each of them, and 4) each is expressed in significant quantities in cells. (edu.au)
We demonstrated this strategy by tagging six different human proteins in both HEK293T and Jurkat cells. (bvsalud.org)
In general, cell adhesion favours cell survival signalling, and integrins are the main receptors responsible for mediating the attachment of different types of cells to matrix proteins. (lu.se)

Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)

Yet, the precise structure-function relationships of properties of the actin network in plants are still to be unravelled, including details of how the network configuration depends upon cell type, tissue type and developmental stage. (biorxiv.org)

In many cases we found statistically-significant differences in actin network properties. (biorxiv.org)

Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)

Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)

Notably, a nucleus-resident actin (R62D NLS ) also regulates SRF's transcriptional activity. (jneurosci.org)

Anatomy17

Organisms10

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes37

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Cytoskeleton13

Filaments6

ABPs1

Cytoplasmic actin2

Microfilaments1

Isoforms4

Monomers2

Neuronal7

Microtubule2

Homology2

Mutants4

Regulation5

Ability to bind1

Neurons2

Modulates2

Translocation1

Myosin4

Transmembrane2

Dynamics1

Outgrowth1

Regulate1

Interaction1

Kinase1

Microscopy3

Complex1

Partially1

Transcription2

Receptor2

Cells3

Factor1

Cell1

Properties1

Role1

Local1

Activity1