Binding of sequence. Medical search. Frequent questions

Is the Subject Area "RNA-binding proteins" applicable to this article? (plos.org)
Here, we identified in the transmembrane helix 7 of human CBRs a consensus sequence already known in other proteins as cholesterol recognition/interaction amino acid sequence and consensus pattern. (unboundmedicine.com)
A ubiquitously expressed sequence-specific transcriptional repressor that is normally the target of signaling by NOTCH PROTEINS. (harvard.edu)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. (wikidata.org)
RNA binding proteins (RBPs) play a vital role in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport, and modulation of mRNA translation and decay. (biomedcentral.com)
DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.g. a genome) and (2) they are bound by DNA-binding proteins. (wikipedia.org)
DNA binding sites are often associated with specialized proteins known as transcription factors, and are thus linked to transcriptional regulation. (wikipedia.org)
DNA binding sites also encompasses the targets of other proteins, like restriction enzymes, site-specific recombinases (see site-specific recombination) and methyltransferases. (wikipedia.org)
DNA binding sites can be thus defined as short DNA sequences (typically 4 to 30 base pairs long, but up to 200 bp for recombination sites) that are specifically bound by one or more DNA-binding proteins or protein complexes. (wikipedia.org)
To quantify the binding affinity of proteins and other molecules to specific DNA binding sites the biophysical method Microscale Thermophoresis is used. (wikipedia.org)
RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
For example, aph promoter combined with different RBS sequences were used for expression of reporter proteins in C. glutamicum (Zhang et al. (springeropen.com)
Myc proteins are basic helix-loop-helix/ leucine-zipper proteins that bind to specific DNA sequences. (uu.nl)
mutant of Max, named dMax, that inhibits sequence-specific DNA binding of Myc proteins. (uu.nl)
Noncoding" sequences are not translated into proteins, and nucleic acids with such motifs need not deviate from the typical shape (e.g. the "B-form" DNA double helix). (wikipedia.org)
For example, many DNA binding proteins that have affinity for specific DNA binding sites bind DNA in only its double-helical form. (wikipedia.org)
Distinct structural bases for sequence-specific DNA binding by mammalian BEN domain proteins. (bvsalud.org)
Together, these studies expand the DNA recognition activities of BEN factors and provide structural insights into sequence-specific DNA binding by mammalian BEN proteins . (bvsalud.org)
Hydration of Lipid-binding Proteins by ten anti-parallel b-strands. (lu.se)

This is an investigation of methods for finding short motifs that only occur in a fraction of the input sequences. (aaai.org)
The binding efficiency was dependent on the sequence, relative orientation, and spacing of the repeated motifs of response elements. (nih.gov)
Currents tools are based on conserved motifs and are applied to peptides with many known binding TCRs. (frontiersin.org)
In addition, the promoter-proximal region between -45 and +30 (relative to the start of initiation) contains two highly conserved motifs, the TATA sequence at around -30 and CA at +1 (ref. 4). (princeton.edu)
Outside of gene exons, there exist regulatory sequence motifs and motifs within the "junk", such as satellite DNA. (wikipedia.org)
Within a sequence or database of sequences, researchers search and find motifs using computer-based techniques of sequence analysis, such as BLAST. (wikipedia.org)
The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)

The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
In both TCRα and TCRβ chains, CDR3 loops have the highest sequence diversity and are the principal determinants of receptor binding specificity. (frontiersin.org)
Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
In contrast to mouse FcRn, which binds to IgGs from multiple different species, human FcRn is surprisingly stringent in binding specificity. (nih.gov)
Here, we have used site-directed mutagenesis in combination with interaction (surface plasmon resonance) studies, with the goal of generating human FcRn variants that more closely resemble mouse FcRn in binding specificity. (nih.gov)
These studies define the molecular basis for the marked difference in binding specificity between human and rodent FcRn, and give insight into how human FcRn recognizes IgGs. (nih.gov)
These findings may have significant implications for the strength, specificity and kinetics of lipid binding. (lu.se)

As this motif is different in the two CBR subtypes, we mutated lysine 402 of CB(1)R into glycine, to obtain a cholesterol recognition/interaction amino acid sequence and consensus similar to that of CB(2)R. Both mutated and wild-type receptors were transiently expressed in human neuronal SH-SY5Y cells, and their localization and functioning were investigated using biochemical assays and immunofluorescence labelling. (unboundmedicine.com)
In biology, a sequence motif is a nucleotide or amino-acid sequence pattern that is widespread and usually assumed to be related to biological function of the macromolecule. (wikipedia.org)

Recently, high-throughput DNA sequencing has enabled large-scale characterization of TCR sequences, producing detailed T cell repertoire (Rep-Seq) ( 11 ). (frontiersin.org)

On the other hand, DNA binding sites for a given transcription factor are usually all different, with varying degrees of affinity of the transcription factor for the different binding sites. (wikipedia.org)

Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)

This method is illustrated for the Ribosome Binding Site Problem, which is to identify the short mRNA 50 untranslated sequence that is recognized by the ribosome during initiation of protein synthesis. (aaai.org)
The effects of dermatotoxic chemicals on activator protein (AP1) DNA binding activity and c-jun and c-fos messenger RNA (mRNA) levels were examined in normal human epidermal keratinocytes. (cdc.gov)

This is also done for the corresponding peptide sequence. (pubmlst.org)
PLEASE NOTE: as some nucleotide (allele) sequences encode previously assigned peptide sequences, due to synonymous substitutions, the allele sequence and peptide sequence identifiers do not necessarily correspond. (pubmlst.org)
The database enables the allele and peptide sequence identifiers to be related to each other and to any alternative names. (pubmlst.org)
We employ new Natural Language Processing (NLP) based methods to predict whether any TCR and peptide bind. (frontiersin.org)
We combined large-scale TCR-peptide dictionaries with deep learning methods to produce ERGO (pEptide tcR matchinG predictiOn), a highly specific and generic TCR-peptide binding predictor. (frontiersin.org)
A set of standard tests are defined for the performance of peptide-TCR binding, including the detection of TCRs binding to a given peptide/antigen, choosing among a set of candidate peptides for a given TCR and determining whether any pair of TCR-peptide bind. (frontiersin.org)
While both chains have been reported to be important to affect binding, we show here that for many TCR-peptide pairs the TCR's binding to target MHC-peptide can be determined with high accuracy using the β-chain only. (frontiersin.org)
The HA1 globular head domain facilitates host-cell binding, which initiates infection, and the HA2 fusion peptide, primed during cleavage activation, facilitates pH-dependent fusion between virus and host-cell membranes ( 27 ). (cdc.gov)

We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
We show also that unstable RAR-RXR heterodimers were formed in solution, and that C-terminal sequences and the DNA-binding domains of both receptors were required for efficient formation of stable heterodimers on response elements. (nih.gov)
Current sequencing methods allow for detailed samples of T cell receptors (TCR) repertoires. (frontiersin.org)
Following specific binding of T cell receptors to viral and bacterial-derived peptides bound to MHC ( 5 ), or from neo-antigens ( 8 - 10 ), the appropriate T cells expand, resulting in the increased frequency of T cells carrying such receptors. (frontiersin.org)
We confirmed that altered receptor-binding avidity of H9N2 viruses, including enhanced binding to human-like receptors, results in antigenic variation in avian influenza viruses. (cdc.gov)

We find treatment with this pyrrole-imidazole (Py-Im) polyamide exhibits sequence selectivity in its repression of AR binding in vivo. (caltech.edu)
This study confirms that the in vivo perturbation pattern caused by a sequence specific polyamide correlates with its in vitro binding preference genome-wide in an unbiased manner. (caltech.edu)
However, the development of DNA microarrays and fast sequencing techniques has led to new, massively parallel methods for in-vivo identification of binding sites, such as ChIP-chip and ChIP-Seq. (wikipedia.org)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
These data support the hypothesis that N-Myc affects neuroblastoma gene expression through the formation of a DNA-binding heterodimeric complex with Max in vivo. (uu.nl)

Differentially changed loci are enriched for sequences resembling ARE half-sites that match the Py-Im polyamide binding preferences determined in vitro. (caltech.edu)
In vitro selection data revealed sequence-specific binding activities of isolated BEN domains from all of these factors. (bvsalud.org)

DNA binding sites are a type of binding site found in DNA where other molecules may bind. (wikipedia.org)
Deep sequencing of RNA molecules cross-linked to immunoprecipitated protein particles (CLIP-seq) revealed. (shengsci.com)
By physically restraining bound molecules against an antibody array, they capture both transient interactions, which yeast two-hybrid or tandem mass spectrometry methods don't, and weak interactions, which are usually obliterated by the wash in protein-DNA microarrays. (genomeweb.com)
Frankfurt am Main, Germany about half of the crystallographically visible water molecules appear to be expelled by the ligand, we find that ligand binding actually increases the number of water molecules within the cavity. (lu.se)

The crystal structures of the conserved ETS domain of SAP-1 bound to DNA sequences from the E74 and c-fos promoters reveal that a set of conserved residues contact a GGA core DNA sequence. (rcsb.org)
Discrimination for sequences outside this core is mediated by DNA contacts from conserved and nonconserved protein residues and sequence-dependent DNA structural properties characteristic of A-form DNA structure. (rcsb.org)

Moreover, due to the limited success of bioinformatics in producing efficient DNA binding site prediction tools (large false positive rates are often associated with in-silico motif discovery / site search methods), there has been no systematic effort to computationally annotate these features in sequenced genomes. (wikipedia.org)
The assay could help accelerate efforts to map where transcription factors bind the genomes of humans and model organisms, Deplancke said. (genomeweb.com)

With the increasing volume of verified RBP binding sites, quite a few studies focused on developing computational prediction models based on the known RBP binding sites. (biomedcentral.com)
To determine from a repertoire whether its host had been exposed to a target, computational tools that predict TCR-epitope binding are required. (frontiersin.org)
This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)

In a paper published in Nature Methods this week, a Switzerland-based team of researchers, led by bioengineer Bart Deplancke of the École Polytechnique Fédérale de Lausanne (EPFL), showcased the assay's ability to determine DNA-binding specificities for transcription factors. (genomeweb.com)

C protein binds strongly to pifO, and weakly to sequences in the ori-1 region. (shengsci.com)
For example, human FcRn does not bind to mouse IgG1 or IgG2a and interacts only weakly with mouse IgG2b. (nih.gov)

GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. (yeastgenome.org)
There has also been work on discriminative de-novo motif finding (e.g., [ 10 ]) where the goal is to find a PWM that discriminates between two sets of sequences. (biomedcentral.com)
Below is a non-exhaustive table of available databases: A collection of DNA binding sites, typically referred to as a DNA binding motif, can be represented by a consensus sequence. (wikipedia.org)
These matrices give information on the frequency of each base at each position of the DNA binding motif. (wikipedia.org)
Usually, however, the first letter is I, and both [RK] choices resolve to R. Since the last choice is so wide, the pattern IQxxxRGxxxR is sometimes equated with the IQ motif itself, but a more accurate description would be a consensus sequence for the IQ motif. (wikipedia.org)

Especially, deep learning is increasingly used in the bioinformatics field by virtue of its ability to learn generalized representations from DNA and protein sequences. (biomedcentral.com)
Deplancke also worked in advanced bioinformatics to derive predictive models for binding interactions. (genomeweb.com)

However, as many experimental settings provide, only beta chains, a binding prediction tool based on these chains is of importance. (frontiersin.org)

Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)

Each novel fHbp nucleotide sequence is assigned a numerical identifier in order of submission. (pubmlst.org)
We have determined the nucleotide sequence of the C gene. (shengsci.com)

Successful recognition of antigenic peptides bound to Major Histocompatibility Complexes (pMHCs) requires specific binding of the TCR to these complexes ( 5 - 7 ), which in turn modulates the cell's fitness, clonal expansion, and acquisition of effector properties ( 7 ). (frontiersin.org)
In contrast, many TCR responses are characterized by a high level of cross-reactivity with single TCRs binding a large number of MHC-bound peptides, and single peptides binding a large number of TCRs ( 16 , 17 ). (frontiersin.org)

The only caveat is that sequence_tolerance will probably not like trying to "design" your ligand, so you'll want to provide a resfile which turns off design to the ligand. (rosettacommons.org)
Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)

The POF protein sequence contains a putative RNA-binding domain. (the-scientist.com)

The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)

The fHbp_allele locus has been renamed to 'fHbp for consistency with other non-full length coding sequence loci, such as 'porA and 'porB. (pubmlst.org)

From then on, DNA binding sites for many transcription factors, restriction enzymes and site-specific recombinases have been discovered using a profusion of experimental methods. (wikipedia.org)
Due to the diverse nature of the experimental techniques used in determining binding sites and to the patchy coverage of most organisms and transcription factors, there is no central database (akin to GenBank at the National Center for Biotechnology Information) for DNA binding sites. (wikipedia.org)
There are, however, several private and public databases devoted to compilation of experimentally reported, and sometimes computationally predicted, binding sites for different transcription factors in different organisms. (wikipedia.org)
REGULATION of eukaryotic messenger RNA transcription is governed by DNA sequence elements that serve as binding sites for sequence-specific transcription factors 1-3 . (princeton.edu)
In terms of predicting where in the genome the transcription factors bind, the models we're able to derive seem to be, in general, actually better than the other assays. (genomeweb.com)

NGS relies on sequencing by synthesis: the sequence of a template DNA strand is determined by synthesizing a complementary strand from fluorescently labeled bases. (acs.org)

Finally, gadB2 and mutant gadB1 were co-expressed under the optimal promoter and RBS combination, thus converted Glu into GABA completely and improved GABA production to more than 25 g/L. This study provides useful promoters and RBS sequences for gene expression in C. glutamicum . (springeropen.com)
A control mutant of dMax that contains a proline residue in the leucinezipper region was unable to bind to N-Myc and did not revert the N-Myc-induced changes in cellular gene expression. (uu.nl)
We used the paradigmatic GATA-PU.1 axis to explore, at the systems level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. (lu.se)
and (4) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. (lu.se)

The binding of trinucleoside diphosphates to long helical rods of tobacco mosaic virus (TMV) protein is shown to depend on base sequence, 5′ AAG 3′ binding being the strongest of the 25 trinucleoside diphosphate sequences measured. (nature.com)

This enhanced binding avidity led to reduced virus replication in primary and continuous cell culture. (cdc.gov)
Efficient virus replication is dependent, in part, on the concerted activities of HA and NA binding and eluting cells and is a balance maintained by matching HA substitutions that alter receptor-binding avidity with changes to NA stalk length and sialidase activity ( 24 - 26 ). (cdc.gov)
An illustration shows the base recognition step in avidity sequencing. (acs.org)
An avidity reagent with a fluorescent tag (green) includes multiple moieties of the same nucleotide, which can match multiple copies of the same template strand (inset box), increasing overall binding strength. (acs.org)

Click molecule labels to explore molecular sequence information. (nih.gov)
Here, we analyze the perturbations to the AR cistrome caused by a minor groove binding molecule that is designed to target a sequence found in a subset of androgen response elements (ARE). (caltech.edu)

To enhance GABA production in C. glutamicum , ribosomal binding site (RBS) sequence and promoter were searched and optimized for increasing the expression efficiency of gadB2 . (springeropen.com)
The initiation of transcription is fundamentally controlled by the promoter elements, while the initiation of translation is mainly affected by the strength of ribosomal binding site (RBS). (springeropen.com)
We have recently shown that the adeno-associated virus type 2 P5 promoter +1 region (P5 + 1 element) binds transcription factor YY1 (ref. 5). (princeton.edu)

This is because they target mostly identical sequences and restriction efficiency decreases abruptly for less similar sequences. (wikipedia.org)

Even though NCBI contemplates DNA binding site annotation in its reference sequences (RefSeq), most submissions omit this information. (wikipedia.org)

A known ferrous iron standard of 105 μmol/L (586 μg/dL) was incubated with serum at a pH of 7.9, which saturates the available binding sites on serum transferrin. (cdc.gov)
There is a need to develop a method for predicting SAM binding sites in a protein for designing drugs against SAM associated disease. (edu.in)
The identification of RBP binding sites is a crucial step in understanding the biological mechanism of post-transcriptional gene regulation. (biomedcentral.com)
However, the determination of RBP binding sites on a large scale is a challenging task due to high cost of biochemical assays. (biomedcentral.com)
Quite a number of studies have exploited machine learning methods to predict binding sites. (biomedcentral.com)
In this paper, we implemented a novel deep neural network model, DeepRKE, which combines primary RNA sequence and secondary structure information to effectively predict RBP binding sites. (biomedcentral.com)
The distributed representations are taken as input of convolutional neural networks (CNN) and bidirectional long-term short-term memory networks (BiLSTM) to identify RBP binding sites. (biomedcentral.com)
Our extensive experimental results show that DeepRKE is an efficacious tool for predicting RBP binding sites. (biomedcentral.com)
Therefore, the identification of RBP binding sites is crucial to understanding the mechanism of biological processes. (biomedcentral.com)
However, these techniques still cost-heavy and time-intensive, which hampers the exploration of RBP binding sites. (biomedcentral.com)
IONMF [ 15 ] proposes a feature representation method of orthogonal matrix eigendecomposition, which integrates the k-mer sequence, secondary structure, gene ontology (GO) information and region type as input into a machine learning model to predict binding sites. (biomedcentral.com)
In analyzing the stability of DNA replication origins in S. cerevisiae (see Stable vs. unstable ARSs in mcm1-1 mutant below) we faced the question of whether one set of sequences has more and/or better binding sites of a particular transcription factor than the other. (biomedcentral.com)
None of these however directly apply to our problem, where the null assumption is that there is "essentially" no difference in the binding sites between the two input sets, even though both might be enriched, deficient, or neutral in sites when compared to "background" sequences. (biomedcentral.com)
The sum of DNA binding sites of a specific transcription factor is referred to as its cistrome. (wikipedia.org)
It has been reported that some binding sites have potential to undergo fast evolutionary change. (wikipedia.org)
DNA binding sites can be categorized according to their biological function. (wikipedia.org)
Thus, we can distinguish between transcription factor-binding sites, restriction sites and recombination sites. (wikipedia.org)
Some authors have proposed that binding sites could also be classified according to their most convenient mode of representation. (wikipedia.org)
On the one hand, restriction sites can be generally represented by consensus sequences. (wikipedia.org)
This makes it difficult to accurately represent transcription factor binding sites using consensus sequences, and they are typically represented using position specific frequency matrices (PSFM), which are often graphically depicted using sequence logos. (wikipedia.org)
The existence of something akin to DNA binding sites was suspected from the experiments on the biology of the bacteriophage lambda and the regulation of the Escherichia coli lac operon. (wikipedia.org)
DNA binding sites were finally confirmed in both systems with the advent of DNA sequencing techniques. (wikipedia.org)
Historically, the experimental techniques of choice to discover and analyze DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay (EMSA). (wikipedia.org)
A more accurate way of representing binding sites is through Position Specific Frequency Matrices (PSFM). (wikipedia.org)
PSFM are usually conceived with the implicit assumption of positional independence (different positions at the DNA binding site contribute independently to the site function), although this assumption has been disputed for some DNA binding sites. (wikipedia.org)

20-fold greater binding to human-like sialic acid receptor analogs. (cdc.gov)
Adaptation to humans requires permissive mutations throughout the genome of avian influenza viruses that affect multiple factors, such as receptor binding, pH stability, virus polymerase activity, innate immune responses, and viral egress ( 17 - 21 ). (cdc.gov)
The MHC class I-related receptor, FcRn, is involved in binding and transporting immunoglobulin G (IgG) within and across cells. (nih.gov)

Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector. (expasy.org)

All models were trained, tested, and evaluated on 145 SAM binding protein chains where no two chains have more than 40\% sequence similarity. (edu.in)
The distributed representations of RNA sequences and secondary structures can effectively detect the latent relationship and similarity between k-mers, and thus improve the predictive performance. (biomedcentral.com)

See also consensus sequence. (wikipedia.org)

Experiments were performed to solve this problem for each of fourteen sequenced prokaryotes, by applying the method to the full complement of genes from each. (aaai.org)
Finally, using electrophoretic mobility shift assay, we have identified YYl-related factors that bind to two other transcription initiators in cellular genes. (princeton.edu)
The authors conclude that dermatotoxic chemical exposure increases the AP1 DNA binding activity and the stimulation of early/immediate genes. (cdc.gov)

Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
NEW YORK (GenomeWeb) - A new microfluidics-driven DNA-protein binding assay has its creators dreaming of launching a startup to cash in on several potential applications. (genomeweb.com)
DNA binding reactions and the electrophoresis mobility shift assay were also conducted. (cdc.gov)

I-LBP, ileal lipid-binding to the aqueous solubility of the fatty acid. (lu.se)

Analysis of binding kinetics reveals that this is primarily due to differences in dissociation rate constants. (elifesciences.org)
We obtained a high-resolution structure of BEND3-BD4 bound to its preferred binding site , which reveals how BEND3 identifies cognate DNA targets and shows differences with one of its non- DNA -binding BEN domains (BD1). (bvsalud.org)

To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)

Now, companies are introducing sequencing platforms that separate fluorescent labeling from the extension of the complementary DNA strand, touting improvements in accuracy that result from optimizing each step. (acs.org)
The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)

For instance, RNAcontext [ 12 ] uses position weight matrix (PWM) of RNA sequence and secondary structure profile to predict the binding preference of RBP. (biomedcentral.com)

We find that the fission yeast homologues of Tristetraprolin/TTP and Pumilio/Puf (Zfs1 and Puf3) interact with Ccr4-Not via multiple regions within low-complexity sequences, suggestive of a multipartite interface that extends beyond previously defined interactions. (elifesciences.org)
As is shown in Table 1 , these methods mostly employ RNA sequence and structural information to predict protein-RNA interactions. (biomedcentral.com)
In order for the home agent to be able to initiate interactions for flow bindings with the mobile node, this document defines new signaling messages and sub-options for Mobile IPv6. (rfc-editor.org)

Result: This manuscript describes a method SAMbinder, developed for predicting SAM interacting residue in a protein from its primary sequence. (edu.in)

It binds as a cofactor to C3b/C4b inactivator (C3bina), which then hydrolyzes the complement fragment C4b. (lu.se)

Under conditions where RAR, RXR, and TR bound poorly as homodimers to various response elements, strongly cooperative RAR-RXR and TR-RXR binding was observed. (nih.gov)

Immunoglobulin J Recombination Signal Sequence-Binding Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)

Because they bind so tightly, Element says avidites work at 100-fold lower concentrations than typical NGS detection bases. (acs.org)
The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)

Observed probabilities can be graphically represented using sequence logos. (wikipedia.org)

Proteins23

Motifs7

Specificity8

Amino Acid Seq2

Characterization1

Affinity1

Target sequences1

MRNA2

Peptide8

Receptors5

Vivo5

Vitro2

Molecules4

Residues2

Genomes2

Computational3

Specificities1

Weakly2

Motif5

Bioinformatics2

Prediction1

Genome-wide1

Nucleotide sequence2

Peptides2

Ligand2

Putative1

Genetic3

Loci1

Transcription factors5

Synthesis1

Gene expression4

Base sequence1

Avidity4

Molecule2

Promoter3

Identical1

NCBI1

Sites25

Receptor3

Recognition1

Similarity2

Consensus1

Genes3

Assay3

Solubility1

Reveals2

RNAs1

Complementary2

Secondary structure1

Interactions3

Residue1

Fragment1

Strongly1

MeSH1

Detection2

Graphically1