Binding of peptides. Medical search. Frequent questions

HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
They show high binding affinity, low toxicity, and are easy to synthesize, all of which makes peptides ideal for use in drug development, and many naturally occurring peptides such as insulin, oxytocin, somatostatin and the antibiotics vancomycin or polymyxin B, are already successfully used like that. (epfl.ch)
First, binding affinity: Good binding requires difficult peptide architectures and amino acid sequences that are perfectly complementary in shape and charge to the surface of their target proteins. (epfl.ch)
The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. (whiterose.ac.uk)
In this work the structure-activity relationship of SP1-7 and EM-2, regarding their affinity to the SP1-7 binding site was elucidated using alanine scans, truncation, and terminal modifications. (avhandlingar.se)
The C-terminal part of both peptides, and especially the C-terminal phenylalanine, was crucial for binding affinity. (avhandlingar.se)
stoichiometry and affinity constant of this peptide toward Escherichia coli cells were determined. (cnr.it)
Full overlapping studies of four antigens identified binding affinity of 103 peptides to seven common HLA-DR molecules (DRB1*0101, 0301, 0401, 0701, 1101, 1301, and 1501). (biomedcentral.com)
As part of our goal of genetically designing these peptides, understanding the binding kinetics and thermodynamics, and using the peptides as molecular erectors, in this report we discuss molecular structural constraints imposed upon the quantitative binding characteristics of peptides with an affinity for inorganics. (itu.edu.tr)
Specifically, we use a high-affinity seven amino acid Pt-binding sequence, PTSTGQA, as we reported in earlier studies and build two constructs: one is a Cys-Cys constrained "loop" sequence (CPTSTGQAC) that mimics the domain used in the pIII tail sequence of the phage library construction, and the second is the linear form, a septapeptide, without the loop. (itu.edu.tr)
A small number of H-bonds between peptide and antibody contribute to the affinity and specificity. (elsevierpure.com)
Copper (II) was used as the reference ligand because it binds the [beta]-amyloid peptide with a high affinity that has been previously measured and reported by many researchers. (ecu.edu)
actually, E146A and D157A substitutions increase the binding affinity to Hpt. (cnr.it)

We demonstrate that PRMT5 uses modular adaptor proteins containing a common binding motif for substrate recruitment, comparable with other enzyme classes such as kinases and E3 ligases. (rcsb.org)
Peptides are short chains of amino acids that can bind to proteins and change their function. (epfl.ch)
The expression of metal-binding proteins or peptides in microorganisms and plants in order to enhance heavy metal accumulation and/or tolerance has great potential. (lu.se)
Several different peptides and proteins have been explored. (lu.se)
Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells. (umassmed.edu)
RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
Peptides presented by MHC-I molecules are mainly intracellular and those presented by MHC-II molecules originate mainly from or extracellular proteins. (biomedcentral.com)
Peptide YY cannot be produced as the result of enzymatic breakdown of crude fish proteins and ingested as a food product. (wikipedia.org)
Haptoglobin (Hpt), a plasma 2-glycoprotein belonging to the family of acute-phase proteins, binds to ApoA-I inhibiting the stimulation of the enzyme LCAT. (cnr.it)
These proteins attach to protein fragments (peptides) outside the cell. (medlineplus.gov)
MHC class II proteins display these peptides to the immune system. (medlineplus.gov)
However, most drugs act as haptens, binding covalently to serum or cell-bound proteins, including peptides embedded in major histocompatibility complex (MHC) molecules. (msdmanuals.com)

In order to determine the specificity I perpared a 6 mM solution of the peptide in binding buffer (MES 100 mM 1 % CHAPS detergent) after binding the protein and washing the column I added 2 ml of the elution buffer (6 mM peptide in wash buffer) and the column (1 ml) swelled up to 5 ml and would not settle. (bio.net)
Bimolecular interaction analysis using a real-time surface plasmon resonance-based assay showed that Compstatin exhibits exclusive specificity for primate C3s and does not bind either to C3s from lower mammalian species or to two structural homologs of C3, human C4 and C5. (nih.gov)
In both TCRα and TCRβ chains, CDR3 loops have the highest sequence diversity and are the principal determinants of receptor binding specificity. (frontiersin.org)
Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
Furthermore, the data provide a rationale for the high target specificity of PBD-C06 and suggest low binding to off-targets, such as unmodified, less toxic Abeta peptides. (vivoryon.com)
The specificity of this binding can be predicted from the amino acid sequence of a peptide. (neueve.com)

the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
We discuss structural features that produce this novel binding motif, including the role of the CaMBD peptide residues Arg-408, Val-409, and Phe-410, which work to provide rigidity to the otherwise flexible central CaM helix joining the N- and C-lobes, ultimately keeping these lobes apart and forcing "head-to-tail" dimerization to attain the requisite N- and C-lobe pairing for CaMBD binding. (rcsb.org)
That being said, there are many ways of cyclizing peptides, and to my knowledge, Peptiderive uses a very narrow definition of "compatible with cyclic geometry": it only considers cyclization schemes involving disulfides between terminal residues of linear peptides when the terminal residues are already close enough to gether to make disulfide closure likely. (rosettacommons.org)
Such vector peptides penetrate cells, in part, because they contain multiple lysine and arginine residues. (utmb.edu)
Supporting Information: LC-MS characterization of GSH-MDI reaction products, Tables of peptides matched to albumin or modified albumin, and MS/MS data on the GSH-MDI modified albumin peptide containing residues 411-428. (cdc.gov)

Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
These peptides (HLA-II T-cell epitopes) are increasingly important as research targets for the development of vaccines and immunotherapies. (biomedcentral.com)

Previously we have identified a 13-residue cyclic peptide, Compstatin, that inhibits C3 activation. (nih.gov)
However, in cyclic peptide predictions, the server did predict only one cyclic peptide based on the middle hotspot in this figure. (rosettacommons.org)
We find that the cyclic peptide of the integral Pt-binding sequence possesses single or 1:1 Langmuir adsorption behavior and displays equilibrium and adsorption rate constants that are significantly larger than those obtained for the linear form. (itu.edu.tr)

I have used ROSIE server to analyze protein-antibody interaction and predict peptide sequences of antibody that would interact with the protein. (rosettacommons.org)
It did make sense to me that the predicted linaer peptides from antibody sequence were chosen from 1st and 3rd hotspots from the figure with highest contact points with the protein. (rosettacommons.org)
Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. (whiterose.ac.uk)
5. The peptide mutation FGF21 and an anti-β-Klotho antibody were biotinylated with EZ-Link Sulfo-NHS-LC-Biotin at the indicated concentrations. (lifetein.com)
Surface plasmon resonance is used to monitor the kinetics of deposition and antibody binding of self-assembled monolayers of cysteine-containing peptides on gold surfaces. (kent.ac.uk)
In these studies, the binding characteristics of a murine version of Probiodrug's lead therapeutic antibody (PBD-C06) against its designated target pGlu-Abeta was analyzed at the molecular level applying co-crystallization and X-ray structure analysis. (vivoryon.com)
The three-dimensional structure of 2H1, a protective monoclonal antibody to Cryptococcus neoformans, has been solved at 2.4 Å resolution, in both its unbound form and in complex with the 12 amino acid residue peptide PA1 (GLQYTPSWMLVG). (elsevierpure.com)
Peptide binding is associated with only minor rearrangements of some side-chains and a small shift in the H2 loop of the antibody. (elsevierpure.com)
The peptide assumes a tightly coiled conformation consisting of one inverse γ-turn and one type II β-turn that serves to place the entire peptide motif, consisting of Thr(P5) Pro(P6), Trp(P8) Met(P9) and Leu(P10), into a depression in the antibody combining site. (elsevierpure.com)
Poor steric complementarity between PA1 and the antibody heavy chain along with the fact that the majority of the interactions between 2H1 and PA1 involve van der Waals interactions with the light chain may explain why this peptide acts as only a partial mimotope of the capsular polysaccharide epitope. (elsevierpure.com)
Bound and free insulin are separated by adding a second antibody, centrifuging, and decanting. (cdc.gov)
C-peptide radioimmunoassay (RIA) is a competitive assay where 125 I-labelled C-peptide competes with C-peptide in the specimen for antibody sites. (cdc.gov)
Bound and free C-peptide is separated by adding a second PEG-accelerated double antibody. (cdc.gov)
The antibody-bound fraction is precipitated and counted. (cdc.gov)
Three phage-displayed 9-mer disulfide-constrained peptides that recognize the BDE 47-polyclonal antibody immunocomplex were isolated. (cdc.gov)
The binding makes the protein-drug complex immunogenic, stimulating antidrug antibody production, T-cell responses against the drug, or both. (msdmanuals.com)

How to do competition binding assay? (lifetein.com)
A series of peptides were synthesized by LifeTein and used for the competition binding assay. (lifetein.com)
We present a new application of the noncompetitive phage anti-immunocomplex assay (PHAIA) by converting an existing competitive assay to a versatile noncompetitive sandwich-type format using immunocomplex binding phage-borne peptides to detect the brominated flame retardant, brominated diphenyl ether 47 (BDE 47). (cdc.gov)

The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
A set of standard tests are defined for the performance of peptide-TCR binding, including the detection of TCRs binding to a given peptide/antigen, choosing among a set of candidate peptides for a given TCR and determining whether any pair of TCR-peptide bind. (frontiersin.org)
Initiation and regulation of immune responses in humans involves recognition of peptides presented by human leukocyte antigen class II (HLA-II) molecules. (biomedcentral.com)
MHC-II molecules present antigenic peptides internalized by professional antigen presenting cells, such as macrophages, dendritic cells, or T lymphocytes. (biomedcentral.com)
Prediction of the binding ability of antigen peptides to major histocompatibility complex (MHC) class II molecules is important in vaccine development. (neueve.com)
For neopeptides to be recognised by CD8+ T cells and induce an immune response , their binding to human leukocyte antigen class I (HLA-I) molecules is a necessary first step. (bvsalud.org)
Together, they form a functional protein complex called an antigen-binding DQαβ heterodimer. (medlineplus.gov)

Recently, phage and cell-surface display libraries have been adapted for genetically selecting short peptides for a variety of inorganic materials. (itu.edu.tr)
Immune responses are regulated and initiated by major histocompatibility complex (MHC) molecules, which bind to short peptides from antigens and display them on the cell surface for the recognition by T cell receptors. (neueve.com)

Biologically active peptides are important for many physiological functions in the human body and therefore serve as interesting starting points in drug discovery processes. (avhandlingar.se)
Understanding the mechanism of action of antimicrobial peptides is pivotal to the design of new and more active peptides. (cnr.it)

Proline-rich antimicrobial peptides (PrAMPs) produced as part of the innate immune response of animals, insects and plants represent a vast, untapped resource for the treatment of multidrug-resistant bacterial infections. (cipsm.de)

Successful recognition of antigenic peptides bound to Major Histocompatibility Complexes (pMHCs) requires specific binding of the TCR to these complexes ( 5 - 7 ), which in turn modulates the cell's fitness, clonal expansion, and acquisition of effector properties ( 7 ). (frontiersin.org)
Both these processes are initiated and regulated by T-helper (Th) cells that recognize antigenic peptides presented by MHC class II (MHC-II) molecules. (biomedcentral.com)

Dear Netters, I'm looking for software that determines whether peptides from a given primary amino acid sequence are likely candidates for binding to the peptide-binding groove of HLA class I (or class II) molecules. (bio.net)
CTLs recognize peptide antigens presented by major histocompatibility complex class I (MHC-I) molecules on infected cells or cancer cells and kill them. (biomedcentral.com)
A distinct characteristic of MHC molecules of either class is a groove that binds peptides in a highly promiscuous manner. (biomedcentral.com)
The presence of the binding core with a uniform length for MHC I molecules makes the prediction of peptide-MHC binding relatively easier. (neueve.com)
These methods can be readily applied to MHC I molecules, since the binding motif is well characterized and most of the natural peptides that bind MHC I molecules are of close to equal length. (neueve.com)
Anthem: a user customised tool for fast and accurate prediction of binding between peptides and HLA class I molecules. (bvsalud.org)
Haptens may also bind directly to class II MHC molecules, directly activating T cells. (msdmanuals.com)

However, the most common form of circulating PYY immunoreactivity is PYY3-36, which binds to the Y2 receptor (Y2R) of the Y family of receptors. (wikipedia.org)
It is thought that the Y1 receptor requires both the C-terminus and N-terminus for recognition, binding and then subsequent activation. (wikipedia.org)
Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice. (lu.se)

Because the four cysteines can be placed in many different ways along the sequence of amino acids, the double-bridging strategy allowed the researchers to generate an enormously large number of structurally diverse peptide architectures. (epfl.ch)
The scientists also found that the four cysteines could be bridged by two linkers in three different ways, giving rise to three different architectures from each single peptide sequence. (epfl.ch)
The 2,2,2-trifluoroethanol titration experiments indicate that TFE has a different effect on the secondary structures of the linear and cyclic versions of the Pt binding sequence. (itu.edu.tr)
We conclude that the presence of the Cys-Cys restraint affects both the conformation and binding behavior of the integral Pt-binding septapeptide sequence and that the presence or absence of constraints could be used to tune the adsorption and structural features of inorganic binding peptide sequences. (itu.edu.tr)
Analysis of truncated P2a analogs showed that P2a sequence can only be slight reduced in length at the N-terminal to preserve the ability of binding to Hpt. (cnr.it)

We combined large-scale TCR-peptide dictionaries with deep learning methods to produce ERGO (pEptide tcR matchinG predictiOn), a highly specific and generic TCR-peptide binding predictor. (frontiersin.org)
However, as many experimental settings provide, only beta chains, a binding prediction tool based on these chains is of importance. (frontiersin.org)
You can use the simple_cycpep_predict application ( https://www.rosettacommons.org/docs/latest/structure_prediction/simple_cycpep_predict ) to determine whether a given design uniquely favours the binding-competent conformation, or whether there'd be a high entropic cost to ordering a disordered peptide on binding. (rosettacommons.org)
We used these data to analyze performance of 21 HLA-II binding prediction servers accessible through the WWW. (biomedcentral.com)
We tried three prediction strategies - the best 9-mer within the longer peptide, the average of best three 9-mer predictions, and the average of all 9-mer predictions within the longer peptide. (biomedcentral.com)
None of the individual predictors was shown to be suitable for prediction of promiscuous peptides. (biomedcentral.com)
The variable length of each binding peptide complicates this prediction. (neueve.com)
Motivated by a text mining model designed for building a classifier from labeled and unlabeled examples, we have developed an iterative supervised learning model for the prediction of MHC class II binding peptides. (neueve.com)
The prediction of MHC class II binding peptides is a difficult classification problem. (neueve.com)
Most epitope prediction tools thus rely on the prediction of such binding. (bvsalud.org)
Here, we present Anthem for accurate HLA-I binding prediction. (bvsalud.org)
In particular, we have developed a user-friendly framework to support the development of customisable HLA-I binding prediction models to meet challenges associated with the rapidly increasing availability of large amounts of immunopeptidomic data. (bvsalud.org)

Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)
Despite the enormous number of inorganic-binding peptides reported and their bionanotechnological utility as synthesizers and molecular linkers, there is still a limited understanding of molecular mechanisms of peptide recognition of and binding to solid materials. (itu.edu.tr)

In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. (whiterose.ac.uk)
PA1 was previously identified as a potential mimotope of the cryptococcal capsular polysaccharide by screening of a phage display peptide library. (elsevierpure.com)

Together, these results highlight the pivotal role of physiologically generated, proteolytically stable, TG2-reactive, multivalent peptides in the immune response to dietary gluten in Celiac Sprue patients. (nih.gov)
If the immune system recognizes the peptides as foreign (such as viral or bacterial peptides), it triggers a response to attack the invading viruses or bacteria. (medlineplus.gov)
This complex displays foreign peptides to the immune system to trigger the body's immune response. (medlineplus.gov)

The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
Furthermore, it showed that although the kinetics of binding of Compstatin to non-human primate C3s were distinctly different from those to human C3, like human C3 its mechanism of binding to non-human primate C3 was biphasic and did not follow a simple 1:1 interaction, suggesting that this binding mechanism could be important for its inhibitory activity. (nih.gov)

synuclein peptide inhibitor across the blood brain barrier (BBB). (neurodegenerationresearch.eu)

We collected test data from four independent studies that involved 721 peptide binding assays. (biomedcentral.com)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)

peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
Following specific binding of T cell receptors to viral and bacterial-derived peptides bound to MHC ( 5 ), or from neo-antigens ( 8 - 10 ), the appropriate T cells expand, resulting in the increased frequency of T cells carrying such receptors. (frontiersin.org)
HLA-II peptide binding studies involve multiple overlapping peptides spanning individual antigens, as well as complete viral proteomes. (biomedcentral.com)

This structure revealed a novel CaM binding motif. (rcsb.org)
Thus, it validates the existence of this novel CaM binding motif. (rcsb.org)

Thus, here, we have determined the crystal structure of CaM complexed with the 25-residue CaMBD peptide without the glycine linker at a resolution of 2.1 A. The structure is essentially identical to the fusion construction which displays CaM bound to the CaMBD peptide as a dimer with an open, elongated conformation. (rcsb.org)
In order to improve the drug-like properties and to find a plausible bioactive conformation, a set of rigidified and methylated dipeptides of different stereochemistry, and analogs with reduced peptide character, were synthesized and evaluated regarding binding, metabolic stability and absorption. (avhandlingar.se)

Moreover, we demonstrate that Bac7 allows initiation complex formation but prevents entry into the elongation phase of translation, and show that it inhibits translation on both mammalian and bacterial ribosomes, explaining why this peptide needs to be stored as an inactive pro-peptide. (cipsm.de)
therefore the need to develop methods aimed at capturing details of the interactions of peptides with bacterial cells is compelling. (cnr.it)

Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)
This paper describes the use of electrospray ionization-mass spectrometry (ESI-MS) to screen two libraries of soluble compounds to search for tight binding inhibitors for carbonic anhydrase II (EC 4.2.1.1). (figshare.com)
Electrospray Ionization Mass Spectrometry (ESI-MS) has become a powerful technique for analyzing protein- and peptide-ligand interactions and quantifying their binding constants (K[subscript]b). (ecu.edu)

Peptide YY (PYY) also known as peptide tyrosine tyrosine is a peptide that in humans is encoded by the PYY gene. (wikipedia.org)

To determine from a repertoire whether its host had been exposed to a target, computational tools that predict TCR-epitope binding are required. (frontiersin.org)

abstract = "Cell-penetrating peptides derived from hydrophilic regions of the homeoprotein Antennapedia (Antp) or the transcription-regulating factor Tat have been used to transport several peptide and oligonucleotide cargoes into the interior of cells. (utmb.edu)

Not all linear peptides in bound complexes are compatible with cyclic geometry. (rosettacommons.org)

EPFL scientists have developed a new type of "double-bridged peptide" that can be tailored to bind tightly to disease targets of interest. (epfl.ch)
Using radiolabeled peptide cargoes covalently linked to Antp- or Tat-related vectors, or to D-Arg heptamers, we found that a significant amount of the label remained tightly bound to plastic and glass surfaces. (utmb.edu)

The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)

Lastly, in-source (gas-phase) dissociation can also occur, meaning the peptide-ligand complex can partially or completely dissociate during ESI. (ecu.edu)
The reference peptide method adds a reference peptide that does not bind the peptide or ligand of interest to solution to account for any nonspecific binding. (ecu.edu)
The reference ligand method adds a reference ligand that does bind the peptide of interest to solution to account for any in-source dissociation that may be occurring. (ecu.edu)
This illustrates the need to combine complementary techniques when studying peptide-ligand systems and quantifying their binding constants. (ecu.edu)

Finally, in silico analysis of the gluten proteome led to the identification of at least 60 putative peptides that share the common characteristics of the 33-mer and the 26-mer peptides. (nih.gov)

And why no other cyclic peptides were predicted based on the other 2 main hotspots in the interaction. (rosettacommons.org)
We previously demonstrated in HEK293 cells that a peptide containing amino acids 76-121 of CaM (CaM-peptide) interrupted the interaction between CaM and mutant huntingtin, reduced mutant huntingtin-induced cytotoxicity and reduced transglutaminase (TG)-modified mutant huntingtin. (ku.edu)
the addition of 10 µM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. (ku.edu)
Here, we investigate the molecular determinants underlining the interaction between Hpt and P2a peptide. (cnr.it)

Upon binding of Ca2+ and calmodulin (Ca2+/CaM) to CaMBD, the autoinhibitory domains dissociate from the catalytic groove, thus activating the enzyme. (rcsb.org)
Importantly, the effect of the CaM-peptide shows selectivity, such that total TG activity is not significantly altered by expression of CaM-peptide nor is the activity of another CaM-dependent enzyme, CaM kinase II. (ku.edu)
To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
PYY1-36 is rapidly processed by the enzyme DPP4 to the 34-amino acid peptide PYY3-36. (wikipedia.org)

Analysis of Ala substitution analogs of Compstatin for their inhibitory activities against mouse and rat complement suggested that the lack of binding of Compstatin to mouse and rat C3s was not a result of sterically hindered access to the binding pocket due to individual bulky side chains or the presence of charge on the Compstatin molecule. (nih.gov)
Biological characterization of the corresponding P2a peptide analogs, Apo146 and Apo157, showed that the two peptides interfere with Hpt binding to HDL and are more effective than P2a peptide in rescue LCAT activity from Hpt inhibition. (cnr.it)

These results, along with atomistic simulations, indicate that the surface charge density of the supported lipid bilayer is neutralized by the attached cationic peptides. (insplorion.com)
Binding of the labeled conjugates was due entirely to the cationic vector moieties. (utmb.edu)

For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)
However, the presence of the extraneous glycine linker cast doubt on the authenticity of this structure as an accurate representation of CN/CaM binding in vivo. (rcsb.org)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)

Beside peptide chemistry the synthetic work included the development of a fast and convenient microwave-assisted protocol for direct arylation of imidazoles. (avhandlingar.se)
Analogous to the 33-mer, the synthetic 26-mer peptide displayed markedly enhanced T cell antigenicity compared to monovalent control peptides. (nih.gov)
Previously, we reported that a synthetic peptide, P2a, binds to and displaces Hpt from ApoA-I restoring the LCAT cholesterol esterification activity in the presence of Hpt. (cnr.it)

Cyclization of peptides with two chemical bridges affords large scaffold diversities. (epfl.ch)

Peptide YY is related to the pancreatic peptide family by having 18 of its 36 amino acids located in the same positions as pancreatic peptide. (wikipedia.org)
Peptide YY3-36 (PYY) is a linear polypeptide consisting of 34 amino acids with structural homology to NPY and pancreatic polypeptide. (wikipedia.org)
Second, proteolytic analysis of a representative gamma-gliadin led to the identification of another multivalent 26-mer peptide that was also resistant to further gastric, pancreatic and intestinal brush border degradation, and was a good substrate of human transglutaminase 2 (TG2). (nih.gov)

The FGF19 peptide was used for alanine scanning mutagenesis. (lifetein.com)

Currents tools are based on conserved motifs and are applied to peptides with many known binding TCRs. (frontiersin.org)
Analysis of binding motifs has suggested that a core of only 9 amino acids within a peptide is essential for peptide-MHC binding. (neueve.com)
The two major forms of peptide YY are PYY1-36 and PYY3-36, which have PP fold structural motifs. (wikipedia.org)

In the present study, a minilibrary of linear, dimeric and dendrimeric peptides containing clusters of basic amino acids was screened with the aim of identifying peptides able to bind HSPGs and thus block RSV attachment and infectivity. (mattek.com)

It was found that a single amino acid mutations in either region were sufficient to abolish β-Klotho binding. (lifetein.com)
Peptide YY is a short (36-amino acid) peptide released from cells in the ileum and colon in response to feeding. (wikipedia.org)

Using the double-bridge approach for both these protein targets, the scientists developed peptides that could bind them efficiently at nanomolar concentrations. (epfl.ch)
Based on the results, Heinis's lab is now applying the peptide format to many other disease targets. (epfl.ch)
They have already developed new, even larger double-bridged peptide libraries and screened them against a range of disease-relevant targets. (epfl.ch)

ATF3 (activating transcription factor 3) protein is a component of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein of transcription factors. (justia.com)

While both chains have been reported to be important to affect binding, we show here that for many TCR-peptide pairs the TCR's binding to target MHC-peptide can be determined with high accuracy using the β-chain only. (frontiersin.org)

We employ new Natural Language Processing (NLP) based methods to predict whether any TCR and peptide bind. (frontiersin.org)

The protein produced from the HLA-DQA1 gene attaches (binds) to the protein produced from another MHC class II gene, HLA-DQB1 . (medlineplus.gov)
One of these haplotypes, known as DQ2, is composed of the protein produced from HLA-DQA1 gene variants known as HLA-DQA1*05:01 or HLA-DQA1*05:05 bound to the protein produced from HLA-DQB1 gene variants known as HLA-DQB1*02:01 or HLA-DQB1*02:02 . (medlineplus.gov)
The other haplotype, known as DQ8, is composed of the protein produced from HLA-DQA1 gene variants known as HLA-DQA1*03:01 or HLA-DQA1*03:02 bound to the protein produced from the HLA-DQB1 gene variant known as HLA-DQB1*03:02 . (medlineplus.gov)

Diabetes mellitus was assessed by measures of plasma glucose, serum insulin, and serum C-peptide in participants aged 12 years and over in the morning examination session only. (cdc.gov)
Insulin in the specimen competes with a fixed amount of 125I-labelled insulin for the binding sites of the specific insulin antibodies. (cdc.gov)

Accordingly, as a result of extensive efforts to solve the problems with the prior art, the present inventors have developed a peptide that binds to ATF3, inhibits the expression of ATF3, and inhibits the signaling pathway of ATF3, and a pharmaceutical composition containing the same, and found that the peptide and the composition can reduce the production of inflammatory cytokines. (justia.com)

Anatomy13

Organisms12

Diseases2

Chemicals and Drugs131

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Psychiatry and Psychology1

Phenomena and Processes59

Disciplines and Occupations3

Technology, Industry, Agriculture6

Information Science4

Health Care2

Affinity14

Proteins14

Specificity7

Residues5

Epitopes2

Cyclic peptide3

Antibody16

Assay3

Antigen7

Short peptides2

Active peptides2

Antimicrobial peptides1

Antigenic2

Molecules7

Receptor3

Sequence5

Prediction12

Recognition2

Phage2

Immune3

Kinetics2

Inhibitor1

Assays2

Antigens3

Motif2

Conformation2

Bacterial2

Mass Spectrometry3

Humans1

Epitope1

Abstract1

Complexes1

Tightly2

Protein Binding1

Ligand4

Characteristics1

Interaction4

Enzyme4

Analogs2

Cationic2

Vivo3

Synthetic3

Cyclization1

Pancreatic3

Mutagenesis1

Motifs3

Dimeric1

Amino acid2

Targets3

Mammalian1

Chains1

Predict1

Gene3

Insulin2

Inhibits1