• Gene expression in eukaryotes is frequently mediated by multiprotein complexes that bind DNA in a sequence-specific manner. (sciencemag.org)
  • ETS domain proteins make up a large family of DNA-binding proteins found in organisms ranging from fruit flies to humans that play a role in a variety of developmental pathways, in oncogenesis, and in viral gene expression ( 5 ). (sciencemag.org)
  • Recently, we showed that GABP is definitely SKF 86002 Dihydrochloride a key component of a gene regulatory network programming B lineage commitment and differentiation by directly regulating Pax5 gene expression (16). (ranscombehouseglynde.com)
  • These mutations are predicted to disrupt consensus ETS transcription factor-binding sites and are correlated with both reduced SDHD gene expression and poor prognosis. (aacrjournals.org)
  • This is the first report demonstrating both physical and functional interactions between GABP and C/EBPalpha and will provide new insights into the molecular basis of myeloid gene expression. (nbrp.jp)
  • However, NRG-1 increased production of the neuroprotective cytokine granulocyte colony-stimulating factor (G-CSF) and the nuclear translocation of the NF-kB p52 subunit, which is associated with the induction of anti-apoptotic and suppression of pro-inflammatory gene expression. (biomedcentral.com)
  • Two GABPα/β heterodimers associate via the COOH-terminal residues of GABPβ, resulting in a heterotetramer that binds to DNA sequences containing two tandem repeats of the GGA motif ( 19 ). (sciencemag.org)
  • These findings suggest that the β1L subunit is a promising new drug target for aggressive glioblastoma and potentially the many other cancers with TERT promoter mutations," said study senior author Joseph Costello , PhD, a leading UCSF neuro-oncology researcher. (healthcanal.com)
  • A 259-base pair fragment of the FCAR promoter is sufficient to direct myeloid expression of a reporter gene and contains functionally important binding sites for CCAAT/enhancer-binding protein alpha (C/EBPalpha) (CE1, CE2, and CE3) and an unidentified Ets-like nuclear protein. (nbrp.jp)
  • The researchers showed that this GABP-β1L-containing form of GABP is required to activate TERT and drive cancer growth, but that it appears not to be essential for healthy cells. (healthcanal.com)
  • When the researchers used multiple techniques, including CRISPR-based gene editing, to eliminate the GAPB1L subunit from glioblastoma cells in laboratory cultures, the cells' growth dramatically slowed. (healthcanal.com)
  • In this study, researchers examine fatty acid binding protein 7 expression in a cohort of 176 primary breast cancers by gene profiling and tissue microarray immunostaining. (mammarycellnews.com)
  • These proteins have in common a conserved DNA-binding domain whose structure, as determined for the ETS proteins Fli-1, Ets-1, and PU.1, has an overall topology similar to that of the "winged helix-turn-helix" family of proteins ( 6-9 ). (sciencemag.org)
  • In many cases, greater DNA target specificity is achieved by the cooperative binding of ETS family members with partner proteins ( 5 ). (sciencemag.org)
  • Deletion mutagenesis identifies the TEL oligomerization domain as a 65 amino acid region which is conserved in a subset of the ETS proteins including ETS‐1, ETS‐2, FLI‐1, ERG‐2 and GABPα in vertebrates and PNTP2, YAN and ELG in Drosophila . (embopress.org)
  • Swapping experiments in which the TEL oligomerization domain was exchanged by the homologous domains of representative vertebrate ETS proteins including ETS‐1, ERG‐2 and GABPα show that oligomerization is a specific property of the TEL amino‐terminal conserved domain. (embopress.org)
  • By lengthy template PCR, the insertion from the geo reporter gene can be mapped between exons 1c and 1b, as well as SKF 86002 Dihydrochloride the … allele inactivated with a gene capture technique to generate GABP-deficient mice (14, 16). (ranscombehouseglynde.com)
  • Eliminating this subunit using CRISPR-based gene editing dramatically slowed the growth of the human cancer cells in lab dishes and when they were transplanted into mice, but removing GABP-ß1L from healthy cells had no discernable effect. (reliawire.com)
  • Consistent with these observations, in vitro binding analyses revealed a physical interaction between the GABPalpha subunit and C/EBPalpha. (nbrp.jp)
  • C/EBPalpha functionally and physically interacts with GABP to activate the human myeloid IgA Fc receptor (Fc alphaR, CD89) gene promoter. (nbrp.jp)
  • Lomax, Margaret I. 1999-05-01 00:00:00 We identified a novel human gene, NOC4 (Neighbor Of COX4), located 5′ to COX4, the gene for cytochrome c oxidase subunit IV, on Chr16q32-ter. (deepdyve.com)
  • Two of the NRF-2/GABP sites are located within 14-bp direct repeats, a conserved feature of mammalian COX4 promoters. (deepdyve.com)
  • 8. The method of claim 1, wherein determining whether the candidate compound disrupts or inhibits the association between the skeletrophin polypeptide and the RNA molecules involves assaying for binding between the skeletrophin polypeptide and the RNA molecules, wherein a reduction or inhibition of binding identifies the candidate compound as a candidate therapeutic agent. (patentsencyclopedia.com)
  • These data suggest that the cell cycle-dependent binding of GABP to the Skp2 promoter plays an important role in the regulation of Skp2 expression and cell cycle progression from G 1 to S phase. (aacrjournals.org)
  • In a recent report, we specifically targeted GABP1L by deleting exon 9 of the gene products are required for normal embryogenesis, whereas loss of GABP1L expression can be compensated for by other GABP isoforms including GABP1S. (ranscombehouseglynde.com)
  • Such nAChRs, termed "fetal" because of their expression early in development, are assembled from five subunits termed α, β, γ, and δ, each encoded by a different gene. (asahq.org)