• It interferes with bacterial cell wall synthesis during active replication, causing bactericidal activity against susceptible organisms. (medscape.com)
  • NFPs are mainly found in bacteria where formyl modified methionine initiates protein synthesis [ 7 ]. (nature.com)
  • Bactericidal antibiotic that inhibits bacterial DNA synthesis, and consequently growth, by inhibiting DNA-gyrase in susceptible organisms. (medscape.com)
  • Doxycycline inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. (nih.gov)
  • Clindamycin inhibits bacterial protein synthesis at the level of the bacterial ribosome. (theodora.com)
  • As a result, we identified a third protein (Mlc) that is required for toxin synthesis by binding to the toxin's DNA. (usda.gov)
  • Furthermore, in low-oxygenated conditions, the previously identified CRP protein works through this newly identified Mlc protein to increase toxin synthesis. (usda.gov)
  • Integration host factor (IHF) binds to and represses the leukotoxin promoter but neither CRP nor IHF is responsible for the anaerobic induction of ltxA RNA synthesis. (usda.gov)
  • Thus, we have undertaken studies to identify other regulators of leukotoxin transcription and to demonstrate how these proteins work together to modulate leukotoxin synthesis. (usda.gov)
  • A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
  • Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)
  • they inhibit bacterial DNA-dependent RNA polymerase, suppressing RNA synthesis. (msdmanuals.com)
  • by binding to the 50S subunit of the ribosome, they inhibit bacterial protein synthesis. (msdmanuals.com)
  • Cephalexin is a first-generation cephalosporin that inhibits bacterial replication by inhibiting bacterial cell-wall synthesis. (medscape.com)
  • The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, specifically the interactions between SSB and the χ subunit of DNA polymerase III in environments of varying salt concentrations. (wikipedia.org)
  • Research has shown that the ssDNA is stabilized by the interaction of SSB and the χ subunit of DNA polymerase III in E. coli, thus preparing for replication by maintaining the correct conformation that increases the binding affinity of enzymes to ssDNA. (wikipedia.org)
  • b FtsZ84 contains a mutation in the GTP binding site that reduces GTP binding and hydrolysis, thereby weakening FtsZ dimerization and subunit-subunit assembly. (biomedcentral.com)
  • c FtsZ84*M206I and FtsZ84*V293I mutations compensate for FtsZ84's GTP binding defect and help to restore subunit-subunit interactions. (biomedcentral.com)
  • The antibiotic binds preferentially to the 50S ribosomal subunit and affects the process of peptide chain initiation. (theodora.com)
  • Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
  • Examples include the HU protein in Escherichia coli, a dimer of closely related alpha and beta chains and in other bacteria can be a dimer of identical chains. (wikipedia.org)
  • HU-type proteins have been found in a variety of bacteria (including cyanobacteria) and archaea, and are also encoded in the chloroplast genome of some algae. (wikipedia.org)
  • Histone-like proteins were unknown to be present in bacteria until similarities between eukaryotic histones and the HU-protein were noted, particularly because of the abundancy, basicity, and small size of both of the proteins. (wikipedia.org)
  • Upon further investigation, it was discovered that the amino acid composition of HU resembles that of eukaryotic histones, thus prompting further research into the exact function of bacterial DNA binding proteins and discoveries of other related proteins in bacteria. (wikipedia.org)
  • Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. (wikipedia.org)
  • H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)
  • In E. coli (and other Gram-negative bacteria) the cyclic AMP receptor protein (CRP/CAP) regulates more than 500 genes. (ku.dk)
  • The bacterial origin of mitochondria suggests that inflammatory pathways found in cytosol-invading bacteria may be relevant to mitochondrial-driven inflammation after MOMP. (nature.com)
  • Some of these mitochondrial DAMPs share similarities with pathogen-associated molecular patterns (PAMPs) found in bacteria and may be derived from their bacterial ancestors. (nature.com)
  • Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
  • Our results were surprising and significant since in another bacteria associated with human health, E. coli, the protein Mlc has the opposite function. (usda.gov)
  • A complex of proteins that forms a receptor for TRANSFERRIN in BACTERIA. (bvsalud.org)
  • Many pathogenic bacteria utilize the transferrin-binding complex to acquire their supply of iron from serum. (bvsalud.org)
  • In molecular biology, bacterial DNA binding proteins are a family of small, usually basic proteins of about 90 residues that bind DNA and are known as histone-like proteins. (wikipedia.org)
  • These dinoflagellate histone-like proteins replace histone in some dinoflagellates and package DNA into a liquid-crystalline state. (wikipedia.org)
  • Histone-like proteins are present in many Eubacteria, Cyanobacteria, and Archaebacteria. (wikipedia.org)
  • Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, and Bacillus phage SPO1 transcription factor, TF1. (illinois.edu)
  • The model suggests that simple differences in the impact of antibiotic exposure does not differ according to the natural history of colonization, interhuman contact, and expo- mechanism of resistance and do not consider the particular nat- sure to -lactam antibiotics explain major differences in the epi- ural history of the colonization of the bacterial species. (cdc.gov)
  • These ferredoxin systems are important in controlling electron transfer across bacterial secondary metabolite production processes which include antibiotic and pigment formation among others. (rsc.org)
  • The integration host factor (IHF), a dimer of closely related chains which is suggested to function in genetic recombination as well as in translational and transcriptional control is found in Enterobacteria and viral proteins including the African swine fever virus protein A104R (or LMW5-AR). (wikipedia.org)
  • PCH has been linked to secondary syphilis, tertiary syphilis, and other viral or bacterial infections. (medlineplus.gov)
  • A study of the viral variant by the University of Washington School of Medicine/UW Medicine found three mutations in the Epsilon coronavirus spike protein that is able to neutralize the potency of antibodies induced by the current vaccines or past COVID-19 infections. (biospace.com)
  • The Scholars of the Podcast reveal ribosomal proteins encoded in viral genomes, and a protein cell receptor for bat influenza viruses. (virology.ws)
  • Protein stabilization upon ligand binding has frequently been used to identify ligands for soluble proteins. (nih.gov)
  • Methods such as differential scanning fluorimetry (DSF) and differential static light scattering (DSLS) have been employed in the 384-well format and have been useful in identifying ligands that promote crystallization and 3D structure determination of proteins. (nih.gov)
  • Developing computational methods to relate receptors by the similarity of their ligands, rather than by protein sequence or structure. (ucsf.edu)
  • FMPs were naturally occurring bacterial ligands that can cause biological effects at nanomolar concentrations. (cdc.gov)
  • Cellular experimental studies confirm that GBP2b was a novel regulatory molecule that promotes M1 macrophage polarization during M. bovis infection and was also an antimicrobial protein that promotes intracellular bacterial clearance. (researchsquare.com)
  • Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is an intracellular pattern recognition receptor that recognizes bacterial peptidoglycan (PG) containing meso-diaminopimelic acid (mesoDAP) and activates the innate immune system. (amrita.edu)
  • Confers responsiveness to intracellular bacterial lipopolysaccharides (lps). (lu.se)
  • Among these hub genes, Guanylate binding protein 2b (GBP2b) was selected as a key gene for further investigation. (researchsquare.com)
  • Carbohydrate - protein inte ractions in nature are often mediated via multivalent binding where the combined strength of multiple receptor - ligand interactions results in a binding that is highly specific and strong. (avhandlingar.se)
  • In addition, docking calculations around the observed binding site for all three states of the receptor, including a model of the desensitized state, showed that barbiturates preferentially stabilize the closed state. (nih.gov)
  • Furthermore, gene co-expression analysis and further experiments showed that GBP2b regulates M1 polarization through activation of Toll-like receptor 4 and nuclear factor-κBp65 protein. (researchsquare.com)
  • One of the mutations affected the receptor binding domain on the spike glycoprotein, which decreased the neutralizing activity of 14 out of 34 neutralizing antibodies that target that domain. (biospace.com)
  • The cAMP receptor protein (CRP) indirectly increases ltxA expression but the intermediary regulator is unknown. (usda.gov)
  • Viruses that infect eukaryotic cells typically bind to a plasma membrane receptor to initiate the reproduction cycle. (virology.ws)
  • Complejo de proteínas que forma un receptor para la TRANSFERRINA en BACTERIAS. (bvsalud.org)
  • Receptor binding studies on guinea-pig pulmonary macrophages, rabbit neutrophils, and human neutrophils utilizing tritium labeled fMLP were undertaken to investigate the possibility they could be used to develop a competitive binding assay. (cdc.gov)
  • The receptor binding patterns indicated the presence of at least three fMLP receptors that would interfere with measuring fMLP in a competitive assay. (cdc.gov)
  • By binding to a specific receptor site, these agents appear to potentiate the effects of gamma-aminobenzoic acid (GABA) and to facilitate inhibitory GABA neurotransmission and other inhibitory neurotransmitters. (medscape.com)
  • Proteins are major chromophores and targets for damage due to their abundance, but the role of proteins in inducing damage to bound DNA, and the effects on DNA-protein interactions is less well characterized. (ku.dk)
  • The cells use complex protein interactions to encapsulate ROS and associated cellular waste and debris. (universetoday.com)
  • In addition to demonstrating different interactions between CRP and Mlc and their effects on periodontal toxin production, we also discovered how the IHF protein decreases toxin production by blocking Mlc binding to the toxin DNA. (usda.gov)
  • Currently, her projects mainly focus on studying BRCA2 and Tel1 related protein-protein interactions involved in DNA damage response pathway, using Cryo-EM and other biophysics methods. (imperial.ac.uk)
  • This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. (nih.gov)
  • Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. (lu.se)
  • Following elution, the protein readily binds DNA, indicating the protein's high affinity for DNA. (wikipedia.org)
  • Streptococcus pneumoniae and Neisseria meningitidis have is specific to the mechanism of resistance to penicillin G com- very similar mechanisms of resistance to penicillin G, which mon to S. pneumoniae and N. meningitidis and mediated by the are mediated by the decreased affinity of penicillin-binding decrease in affinity of their PBPs. (cdc.gov)
  • Biacore biosensor assays indicated that PSGL - 1/mIgG 2b carrying the blood group P1 determinant in multiple copies bound with high affinity to Stx1 and Stx2, while PSGL - 1/mIgG 2b presenting multiple copies of Sia α 2,3Gal on different O - linked cores bound with high affinity to avian influenza H5. (avhandlingar.se)
  • Independent evidence shows that it binds iron directly, although with very distinct features and low affinity. (cnr.it)
  • We demonstrate that the protein has low cation specificity and contains multiple binding sites able to chelate divalent and trivalent metals with low affinity. (cnr.it)
  • Doxycycline has a high degree of lipoid solubility and a low affinity for calcium binding. (nih.gov)
  • This unique 96-well metal-chelate affinity-chromatography module strongly and selectively binds His-tagged proteins. (qiagen.com)
  • Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. (lu.se)
  • Antibody-antigen affinity is often evaluated with isolated proteins. (lu.se)
  • In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution. (lu.se)
  • Kumra Ahnlide, V & Nordenfelt, P 2023, Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry . (lu.se)
  • Here, we have carried out an extensive study of the binding properties of CyaY, the bacterial ortholog of frataxin, to different divalent and trivalent cations, using NMR and X-ray crystallography. (cnr.it)
  • Here we show, using X-ray crystallography, molecular dynamics simulations and in vitro and in vivo functional assays, that MlaA is a monomeric α-helical OM protein that functions as a phospholipid translocation channel, forming a ~20-Å-thick doughnut embedded in the inner leaflet of the OM with a central, amphipathic pore. (nature.com)
  • Jie used to work on influenza A viruses to understand the influence of virus evolution on its binding property using X-ray crystallography and bio-layer interferometry when she did her PhD in Francis Crick Institute. (imperial.ac.uk)
  • In this thesis we have produced proteins that are densely decorated with carbohydrate determinants in order to study the glycosylation capacity of cell lines (paper I) and generate efficient binders of antibodies (paper II), bacterial toxins (paper III) and virus receptors such as the influenza hemagglutinin (paper IV). (avhandlingar.se)
  • Antibodies attach (bind) to red blood cells. (medlineplus.gov)
  • Anti-PEG antibodies enriched in the protein corona of PEGylated nanocarriers impact the cell uptake. (mpg.de)
  • The next step will be to identify what factors the symptom-inducing antibodies bind to. (biospace.com)
  • Q-bodies are artificial antibodies which become fluorescent on binding to their target. (titech.ac.jp)
  • Nucleotide-binding oligomerization domain proteins (NODs) are modular cytoplasmic proteins implicated in the recognition of peptidoglycan-derived molecules. (hindawi.com)
  • Several PRRs have been identified, among them are the TLRs (Toll-like receptors), NLRs (nucleotide-binding and oligomerization domain-like receptors), and RLR (retinoic-acid-inducible gene-1-like receptors) [ 1 ]. (hindawi.com)
  • In E. coli, H-NS binds to a P1 promoter decreasing rRNA production during stationary and slow growth periods. (wikipedia.org)
  • The corresponding increase in biomass can deliver in some cases up to 10 mg of pure His-tagged protein per well (see table and figure Milligram amounts of pure His-tagged proteins per well. Vector constructs for the expression of His-tagged proteins were transformed into E. coli , plated on selective medium, and colonies were picked for inoculating 25 ml cultures. Expression of 6xHis-tagged proteins was induced with IPTG for 2-4 hours. Cells were pelleted in 24-well blocks and processed on the BioRobot 3000 using 200 µl Ni-NTA Superflow resin per well. 5 µl (0.9%) of the first elution fraction was loaded for SDS-PAGE and proteins were visualized by Coomassie staining. G : Green Fluorescent Protein (29 kDa); T : T7 RNA Polymerase (100 kDa); S :  E. coli GroES (12 kDa). Some endogenous GroEL is copurified; C : E. coli chloramphenicol acetyltransferase (28 kDa); L : E. coli GroEL (60 kDa); α : human tumor necrosis factor α(18 kDa); E : E. coli GroES purified as a complex with co-overexpressed nontagged GroEL (12 and 60 kDa); 10 : Saccharomyces cerevisiae Cpn-10 (10 kDa). M : markers. "> Milligram amounts of His-tagged proteins per well ) allowing multiple assays to be carried out on the same batch of protein and reducing the total number of protein preps required, see also figure Automated expression clone screening. Medium-scale purification for expression-clone screening and protein characterization. A mixture of vector constructs for the expression of proteins of 12, 15, and 28 kDa, and a 48/50 kDa heterodimer was transformed into E. coli , plated on selective medium, and 96 colonies were picked at random for inoculating 5 ml cultures. Expression of 6xHis-tagged proteins was induced with IPTG overnight. Cells were pelleted and lysed and 6xHis-tagged proteins purified. 5 µl of each elution fraction was loaded for SDSPAGE analysis; proteins were visualized by Coomassie staining. "> Automated expression clone screening . (qiagen.com)
  • Deformylases were isolated from Escherichia-coli in an attempt to develop a competitive binding assay for detecting fMLP. (cdc.gov)
  • However, finding a generic method that is applicable to membrane proteins has been a challenge as the high hydrophobicity of membrane proteins and the presence of detergents essential for their solubilization interfere with fluorescence-based detections. (nih.gov)
  • Here the authors used MsbA (an adenosine triphosphate binding cassette transporter), CorA (a Mg(++) channel), and CpxA (a histidine kinase) as model proteins and show that DSLS is not sensitive to the presence of detergents or protein hydrophobicity and can be used to monitor thermodenaturation of membrane proteins, assess their stability, and detect ligand binding in a 384-well format. (nih.gov)
  • Some attach to bacterial outer membrane proteins, while others attach to appendages such as pili or flagella. (virology.ws)
  • Tetracyclines are readily absorbed and are bound to plasma proteins in varying degree. (nih.gov)
  • Squaric Ester‐Based Nanogels Induce No Distinct Protein Corona but Entrap Plasma Proteins into their Porous Hydrogel Network. (mpg.de)
  • Binding does not involve cavities or pockets, but exposed glutamates and aspartates, which are residues that are unusual for iron chelation when not assisted by histidines and/or cysteines. (cnr.it)
  • In this study we show that exposure of isolated dimeric CRP-cAMP to UV modifies specific Met, Trp, Tyr, and Pro side-chains, induces inter-protein Tyr63-Tyr41 cross-links, and decreases DNA binding via oxidation of Met114/Pro110 residues in close proximity at the CRP dimer interface. (ku.dk)
  • UV exposure also modifies DNA-bound cAMP-CRP, with this resulting in DNA cleavage at specific G/C residues within the sequence bound to CRP, but not at other G/C sites. (ku.dk)
  • in these processes, bacterial DNA binding proteins have an architectural role, maintaining structural integrity as transcription, recombination, replication, or any other DNA-dependent process proceeds. (wikipedia.org)
  • When H-NS is bound with RNA Polymerase to the promoter region, there are structural differences in the DNA that are accessible. (wikipedia.org)
  • In this study, we present the first X-ray structures of barbiturates bound to GLIC, a cationic prokaryotic pLGIC with excellent structural homology to other relevant channels sensitive to general anesthetics and, as shown here, to barbiturates, at clinically relevant concentrations. (nih.gov)
  • The identification of this pore binding site sheds light on the mechanism of barbiturate inhibition of cationic pLGICs and allows the rationalization of several structural and functional features previously observed for barbiturates. (nih.gov)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • Her work here is focussed on providing structural and mechanistic insight into the activity of diadenylate cyclase DacA, an essential membrane protein responsible for the production of c-di-AMP in Staphylococcus aureus . (imperial.ac.uk)
  • Luke completed his D.Phil in Clinical Medicine at Oxford University (Merton College), but his research career has predominantly focused on investigating protein function using structural biology as a means to provide a mechanistic understanding in life and in disease. (imperial.ac.uk)
  • Further, several in vivo studies have demonstrated a role for Nod1 and Nod2 in host defense against bacterial pathogens. (hindawi.com)
  • Standard methodology for the susceptibility testing of the potential bacterial vaginosis pathogens, Gardnerella vaginalis, Mobiluncus spp. (theodora.com)
  • The Gram-negative bacterial outer membrane (OM) is a unique bilayer that forms an efficient permeation barrier to protect the cell from noxious compounds 1 , 2 . (nature.com)
  • The STAS domain is found in the C-terminal region of sulphate transporters and bacterial anti-sigma factor antagonists. (nih.gov)
  • Investigators from NYU Grossman School of Medicine identified a connective tissue protein that otherwise supports the framework of organs, but also helps the immune system response to bacterial infections. (biospace.com)
  • Nod1 and Nod2 are NLR proteins that trigger nuclear factor- κ B (NF- κ B) signaling in response to bacterial peptidoglycan. (hindawi.com)
  • Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6kDa from ASFV infected swine macrophages at late times post-infection. (illinois.edu)
  • BACKGROUND: Bacterial infection causes accumulation of neutrophils that release antimicrobial proteins including heparin-binding protein (HBP). (lu.se)
  • One such example is diphtheria toxin, which is produced by Corynebacterium diphtheriae and causes diphtheria, a serious and potentially fatal bacterial infection of the nose and throat. (scitechdaily.com)
  • Bacterial infection from medical devices is a major problem and accounts for an increasing number of deaths as well as high medical costs. (mdpi.com)
  • One way to prevent infection is by modifying the surface of the devices in such a way that no bacterial adhesion can occur. (mdpi.com)
  • P - selectin glycoprotein ligand - 1 (PSGL - 1) is a mucin - type protein that is heavily substituted with O - glycans. (avhandlingar.se)
  • A longstanding effort to do so is by exploiting protein structures to predict new reagents and therapeutic leads (structure-based ligand discovery). (ucsf.edu)
  • With modern genetic engineering tools, it is now possible to modify microorganisms so that their production of industrially useful proteins-such as those used in biopharmaceuticals-is enhanced. (titech.ac.jp)
  • Bacterial Pathogenesis: Methods and Protocols (2 ed., pp. 251-259). (lu.se)
  • Bacterial Pathogenesis: Methods and Protocols. (lu.se)
  • Bacterial Peptidoglycan with Amidated Meso-diaminopimelic Acid Evades NOD1 Recognition: an Insight into NOD1 Structure-Recognition. (amrita.edu)
  • Upon peptidoglycan detection, Nod1 and Nod2 recruit and associate with the adaptor protein Rip2, triggering proinflammatory pathways such as NF- κ B and the mitogen-activated protein (MAP) kinases p38, JNK, and ERK [ 4 ]. (hindawi.com)
  • HU is a small (10 kDa) bacterial DNA-binding protein, which structurally differs from a eukaryotic histone but functionally acts similarly to a histone by inducing negative supercoiling into circular DNA with the assistance of topoisomerase. (wikipedia.org)
  • The team subsequently observed that two structurally different bacterial toxins also employed this same mechanism. (scitechdaily.com)
  • MlaA represents a lipid transport protein that selectively removes outer leaflet phospholipids to help maintain the essential barrier function of the bacterial OM. (nature.com)
  • Therefore, -lactam antibiotics, such as penicillin G, bind to PBPs in better understanding of S. pneumoniae resistance selection and the bacterial cell wall. (cdc.gov)
  • The mucin - type protein was used as a probe to analyze the O - glycosylation capacity of the se cell lines, which today are used for the commercial production of recombinant proteins and vaccine co mponents. (avhandlingar.se)
  • The glycosylation of PSGL - 1/mIgG 2b may be tailored by producing the protein in genetically engineered cell lines. (avhandlingar.se)
  • To our knowledge, p11.6 is the first bacterial histone-like DNA-binding protein found in an animal virus or eukaryotic cell system. (illinois.edu)
  • The study identified the ways in which proteins cross a cell membrane, a finding that could create a scientific foundation for better ways of delivering drugs into cells in the future, or for treating illnesses caused by bacterial toxins. (scitechdaily.com)
  • Pei's research team at Ohio State has spent years trying to understand how biomolecules such as bacterial toxins get inside a human cell, with the goal of finding ways to get medications into those cells. (scitechdaily.com)
  • Researchers have known how small molecules penetrate cell membranes, typically by binding to the membrane and then diffusing through it. (scitechdaily.com)
  • The team witnessed the budding-and-collapse in live cells through confocal microscopy, an imaging technique that allowed them to focus in on what was happening inside the cells, and on the cell membranes, with these specific proteins. (scitechdaily.com)
  • For instance, mtDNA and bacterial DNA are highly similar thereby activating similar cell autonomous immune signalling pathways. (nature.com)
  • In this review, we discuss how mitochondria can initiate inflammation during cell death highlighting parallels with bacterial activation of inflammation. (nature.com)
  • Is mitochondrial-driven inflammation during cell death driven by bacterial-like DAMPs? (nature.com)
  • Previous work identified two proteins in the bacterial cell (IHF, CRP) that directly affect toxin level by either directly or indirectly binding to the cellular DNA code responsible for toxin production. (usda.gov)
  • He is working on developing a workflow for prototyping proteins using cell-free systems and machine learning. (imperial.ac.uk)
  • Cleared bacterial cell lysates flow onto a filter plate preloaded with Ni-NTA Superflow by the BioRobot workstation. (qiagen.com)
  • They are commonly referred to as histone-like and have many similar traits with the eukaryotic histone proteins. (wikipedia.org)
  • Eukaryotic histones package DNA to help it to fit in the nucleus, and they are known to be the most conserved proteins in nature. (wikipedia.org)
  • In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
  • And it is also how bacterial toxins enter human cells and wreak havoc. (scitechdaily.com)
  • Reference: "Bacterial Toxins Escape the Endosome by Inducing Vesicle Budding and Collapse" by Ashweta Sahni and Dehua Pei, 23 September 2021, ACS Chemical Biology . (scitechdaily.com)
  • Genomic reorganization, such as rearrangements and inversions, influences how genetic information is organized within the bacterial genomes . (bvsalud.org)
  • The other two mutations affected the N-terminal domain on the spike protein. (biospace.com)
  • The researchers put their method to the test by screening a huge library of bacterial strains created to produce FGF9, a human cytokine, and subjected to circumstances that cause random mutations. (titech.ac.jp)
  • First, analyses of ltxA RNA expression from defined leukotoxin promoter mutations in the chromosome identify -69 to -35 as the key control region and indicate that an activator protein modulates leukotoxin transcription. (usda.gov)
  • the protein is involved in stabilizing the lagging strand as well as interacting with DNA polymerase III. (wikipedia.org)
  • In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. (wikipedia.org)
  • It has been found that H-NS and RNA polymerase both bind to the P1 promoter and form a complex. (wikipedia.org)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • Guanylate-binding protein 1 (GBP1/GBP2b) is a member of the large family of IFN-γ-inducible GTPases. (researchsquare.com)
  • The Ni-NTA Superflow 96 BioRobot Kit provides automated purification of up to 600 µg recombinant protein from 96 samples in parallel (standard protocol, 5 ml culture volume per sample). (qiagen.com)
  • Deficiency of the small mitochondrial protein frataxin causes Friedreich's ataxia, a severe neurodegenerative pathology. (cnr.it)
  • Some of these mitochondrial-activated inflammatory pathways can be traced back to the bacterial ancestry of mitochondria. (nature.com)
  • Mitochondrial-derived DAMPs can be bacterial-like. (nature.com)
  • Due to its bacterial ancestry, mitochondrial formylation of methionine is required for translation initiation of mtDNA-derived mRNA [ 7 ] and will therefore be recognised by the same formyl peptide receptors. (nature.com)
  • We describe a mathematical model of the emergence and diffusion of bacterial resistance in the community. (cdc.gov)
  • The penicillinase-resistant, or second-generation, penicillins are semisynthetic modifications of natural penicillins that are resistant to bacterial enzyme beta-lactamase, which accounts for typical penicillin resistance. (medscape.com)
  • While working on the team's other projects, Sahni noticed that some fragments of proteins, known as peptides, cross membranes by pushing against them. (scitechdaily.com)
  • In conclusion, PSGL - 1/mIgG 2b carrying specific carbohydrates is a versatile tool that can be used in a range of applications where the multivalency confers a biologically relevan t binding. (avhandlingar.se)
  • Wait until Abx Tx complete to administer live bacterial vaccine. (medscape.com)
  • CEFTIN tablets are indicated for the treatment of pediatric patients (who can swallow tablets whole) with acute bacterial otitis media caused by susceptible strains of Streptococcus pneumoniae , Haemophilus influenzae (including β-lactamase-producing strains), Moraxella catarrhalis (including β-lactamase-producing strains), or Streptococcus pyogenes . (rxlist.com)
  • CEFTIN for oral suspension is indicated for the treatment of pediatric patients aged 3 months to 12 years with acute bacterial otitis media caused by susceptible strains of Streptococcus pneumoniae , Haemophilus influenzae (including β-lactamase-producing strains), Moraxella catarrhalis (including β-lactamase-producing strains), or Streptococcus pyogenes . (rxlist.com)
  • A unique method to screen large-scale libraries for industrially useful bacterial strains was recently developed by Tokyo Tech researchers. (titech.ac.jp)
  • The simple approach combines biosensors and microfluidics to quickly identify mutant strains that secrete large amounts of industrially useful proteins, opening the doors to more applications, like reasonably priced biopharmaceuticals. (titech.ac.jp)
  • Nonetheless, the traditional method of genetically engineering bacterial strains for high protein production is extremely time consuming. (titech.ac.jp)
  • This is because it relies on introducing genetic modifications in individual strains and evaluating the effectiveness of protein production. (titech.ac.jp)
  • As an alternative, researchers sometimes rely on screening of large-scale libraries for identifying strains which secrete a high amount of protein. (titech.ac.jp)
  • This enables the extraction of only those strains that are the best at producing the desired protein. (titech.ac.jp)
  • The study was led by Associate Professor Tetsuya Kitaguchi from Tokyo Institute of Technology (Tokyo Tech), Japan, and was conducted in collaboration with Ajinomoto Co., Inc. The innovative method, which combines microfluidics and versatile biosensing to quickly identify enhanced bacterial strains that produce the highest amount of a desired protein, is reported in their study published in the journal Small on April 24, 2023. (titech.ac.jp)
  • The plot shows a comparison of the amount of the desired protein produced between 24 randomly picked strains before sorting and 24 strains picked from the top 50 performers. (titech.ac.jp)