Bind to transcription factors e2f1 transcription factor. Medical search. Frequent questions

These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. (thermofisher.com)
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. (nih.gov)
Specific factors are involved in the regulation of circRNA biogenesis [ 1 ], a process that involves the spliceosomal splicing mechanism wherein RNA-binding proteins participate in the formation of circRNAs by binding to and probably stabilizing the back-splicing process. (biomedcentral.com)
The LxCxE motif binds to all pocket proteins and is present in chromatin regulators such as HDAC and KDM5A and in viral proteins. (eu.org)
The LxSxE motif binds to the same highly conserved shallow groove in the B subdomain of pocket proteins as the LIG_RB_LxCxE_1 motif. (eu.org)
The expression of a gene goes through many steps, the first of which is transcription (i.e.), the 'message' encoded in the DNA of the gene is transcribed into RNA signals by proteins called transcription factors. (biovoicenews.com)
We found genes for１）Zinc finger proteins are large and diverse group of proteins in which the DNA-binding region contains projections ('fingers') with Cys and/or His residues folding around zinc atom. (i-tmb.com)
It is widely believed that in mammalian cells the cellular transcription factor (DRTF1/E2F integrates cell-cycle events with the transcription apparatus by interacting with important regulators of the cell cycle, such as the retinoblastoma gene product (pRb) and related proteins, cyclins, and cyclin-dependent kinases. (ox.ac.uk)
Here, we have defined DRTF1/E2F in Xenopus laevis that, like its mammalian counterpart, specifically binds to the E2F site, is regulated during development, and interacts with pRb and related proteins. (ox.ac.uk)
Both DP-1 and DP-2 synergistically interact with members of the E2F family of proteins, E2F-1, E2F-2, and E2F-3, to generate DNA binding complexes that specifically recognize the E2F site and functionally interact with E2F-1 in E2F site-dependent transcriptional activation of cellular genes. (ox.ac.uk)
This study therefore defines a new family of transcription factors, the DP proteins, members of which can interact combinatorially with E2F proteins to generate an array of DNA binding complexes that integrate cell-cycle progression with the transcription apparatus through the E2F binding site. (ox.ac.uk)
The increased expression of Y box binding proteins in somatic cells is associated with cell proliferation and transformation. (avivasysbio.com)

Via a feedback loop, SIRT1 regulates the activity of promoter-bound transcription factors through deacetylation. (biomedcentral.com)
We revealed that the hypermethylation of adiponectin promoter inhibits adiponectin transcription. (nature.com)
they have shown that E2F1 fails to bind the ESRP1 promoter in oxygen-deprived breast cancer cells, thereby downregulating the expression of ESRP1. (biovoicenews.com)
The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. (affbiotech.com)
Retinoblastoma protein (Rb) associates with E2F1 and then recruits co-factors to the promoter region of target genes to suppress transcription. (kenstoreylab.com)

This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. (thermofisher.com)
E2F1 binds preferentially RB1 in a cell-cycle dependent manner. (nih.gov)

Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). (nih.gov)
We speculated that WIF1 and sFRP4 expressed in mature osteoblasts inhibited the binding of FZD1 to Wnt ligand in BMMSCs, thereby further inhibiting osteogenic differentiation of BMMSCs. (ijstemcell.com)
Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. (affbiotech.com)
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. (cusabio.com)
Growth and differentiation processes are also minimized during hibernation and ETS transcription factors are important regulators of these. (kenstoreylab.com)
The selective recognition of double-stranded DNA sequences by transcription factors is used by cells to respond to environmental changes, regulating which genes are switched on or off, and is used to control differentiation from one cell type into another. (webflow.io)

Positively regulates transcription of RRP1B (PubMed:20040599). (nih.gov)
Dr. Shukla and his team found that in tumour tissues, a transcription factor called E2F1 that regulates transcription of ESRP1 gets upregulated, thereby increasing its expression and eventually leading to excessive growth of breast cancer cells. (biovoicenews.com)

E2F1 is involved in DNA REPAIR and APOPTOSIS . (nih.gov)
El factor E2F1 está relacionado con la REPARACIÓN DEL ADN y con la APOPTOSIS. (bvsalud.org)
It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA . (lookformedical.com)
An APOPTOSIS-regulating protein that is structurally related to CASPASE 8 and competes with CASPASE 8 for binding to FAS ASSOCIATED DEATH DOMAIN PROTEIN. (lookformedical.com)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The following product was used in this experiment: E2F1 Monoclonal Antibody (KH95) from Thermo Fisher Scientific, catalog # 32-1400, RRID AB_2533065. (thermofisher.com)
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)
Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. (avivasysbio.com)

and cell-cell contact, mobilize stored p27, enabling it to bind and inhibit cdk2 (ref. 6). (medscape.com)
The human sulfatase 1 (hSulf-1) gene encodes an endosulfatase that functions to inhibit the heparin-binding growth factor signaling, including the basic fibroblast growth factor (bFGF)-mediated pathway, by desulfating the cell surface heparan sulfate proteoglycans (HSPGs). (oncotarget.com)
Our research further suggests that E2F1 could be a molecular drug target to inhibit the growth of both normoxic as well as hypoxic breast cancer cells. (biovoicenews.com)
CKIs bind and inhibit the activity of cyclin/Cdk complexes and negatively regulate cell cycle progression (reviewed in Refs. (aacrjournals.org)
They can bind and inhibit a broad range of cyclin/Cdk complexes, with a preference for those containing Cdk2 ( 17, 18 ). (aacrjournals.org)

Retinoblastoma protein recruits histone deacetylase to repress transcription. (nih.gov)
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A and activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis. (nih.gov)

For example, Cerebellar degeneration-related protein 1 antisense RNA (CDR1as) participates in the regulation of genes that contain miR-7-binding sites through competitive interaction with miR-7, whereas circFOXO3 acts as an accelerator of cardiac senescence by interacting with the transcription factor E2F1 and the anti-senescent protein ID-1. (biomedcentral.com)
circRNAs can also regulate the transcription of their parental genes specifically. (biomedcentral.com)
Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes. (affbiotech.com)
2 transcription factors FOXO3 and MYBL2, and 2 miRNAs hsa-miR-21-5p and hsa-miR-30c-5p may be involved in the regulation of the expression of these differentially expressed genes. (cusabio.com)
The carbohydrate response element-binding protein (ChREBP) regulates genes that are responsible for converting excess dietary carbohydrate (glucose) into triglycerides. (kenstoreylab.com)
oppositely, transcription of downstream genes (cMyc, Bcl2) was suppressed. (kenstoreylab.com)
An enduring challenge in drug discovery is the design of synthetic transcription factors that correct aberrant transcription - i.e., turn on or off - of diseased genes. (webflow.io)
The group is developing a new generation of synthetic transcription factors that selectively recognise DNA sequences and modulate the expression of target genes. (webflow.io)
In that profile, we paid attention for genes for transcription. (i-tmb.com)
And genes for DNA maintenance and remodeling, cell cycle, transcription factors or transcription factor subunits are also essential for the expression of genes for metabolism, cell growth, and cytoskeleton. (i-tmb.com)
Androgen receptor (AR), a transcription factor part of the nuclear receptor family, is the primary effector of testosterone and activates genes involved in growth, metabolism, and pro-survival signaling [ 6 ]. (ijbs.com)
The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. (cancerindex.org)
Expression of DNA restore genes in oral squamous cell carcinoma utilizing reverse transcription-quantitative polymerase chain response Goal: The goal of this examine was to judge the expression of DNA restore genes in circumstances of oral squamous cell carcinoma (OSCC). (nfkb-p65.net)
The E2F1 transcription issue and RB tumor suppressor are greatest identified for his or her roles in regulating The expression of genes vital for cell cycle development however, additionally they have transcription-independent features that facilitate DNA restore at websites of injury. (nfkb-p65.net)

Inhibition of DNA binding by the phosphorylation of poly ADP-ribose polymerase protein catalysed by protein kinase C. Biochem Biophys Res Commun 187 , 730-736. (nih.gov)
A phosphorylation downstream to the core motif acts as a switch that binds to a positively charged pocket only present in p107/p130. (eu.org)
However, Ser28 phosphorylation allows pS28 to bind to a positively charged pocket formed by R869, S876, Y879 and R880, increasing binding affinity for p107 10-fold. (eu.org)

In vivo binding of E2F1 on target gene promoters was demonstrated by ChIP assays and E2F1 CRISPR-Cas9 knockdown. (biomedcentral.com)
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. (affbiotech.com)

Hypophosphorylated Rb binds E2F and recruits histone deacetylases and methyltransferases to repress the expression of E2F controlled gene expression. (eu.org)

[ 5 ] During periods of cell proliferation, p27 remains in storage by binding to cdk4 or cdk6 in a non-inhibitory fashion. (medscape.com)
The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)

Post-transcriptional control of SIRT1: upon oxidative stress, SIRT1 mRNA is degraded due to a checkpoint-kinase-2-mediated dissociation of the RNA binding protein HuR. (biomedcentral.com)
Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. (ugent.be)
Finally, we observed that 5-AZA and Vitamin C led to an increased expression of Hepatocyte nuclear factor 4α (HNF4α) and E-Cadherin and a significant down regulation of Snail1 (SNAIL), the key transcriptional repressor of E-Cadherin. (cancerindex.org)
Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta DNA topoisomerase II beta (TOP2B) has a job in transcriptional regulation. (nfkb-p65.net)

The splicing factor Quaking (QKI) has been demonstrated to regulate a variety of circRNAs that are involved in the epithelial-mesenchymal transition (EMT) in humans, exemplifying a role for circRNA regulation in a biological process that could have implications for diseases such as cancer [ 1 ]. (biomedcentral.com)
In this context, the sirtuins have been reported to play a key role, which is mostly based on their requirement of NAD + as a co-factor for enzymatic activity, which in turn demonstrates the crucial link between sirtuins and the energy-dependent regulation of gene expression. (biomedcentral.com)
Transcription factor regulation in mammalian hibernation: the thirteen-lined ground squirrel as a model. (kenstoreylab.com)
This thesis investigated three transcription factor pathways and their regulation and roles in hibernation using tools including immunoblotting of protein and phospho-protein levels, analysis of nuclear versus cytoplasmic distribution, binding to DNA, PCR to quantify mRNA transcripts, and gene sequencing. (kenstoreylab.com)
Contribution of the IGCR1 regulatory element and the 3'Igh CTCF-binding elements to regulation of Igh V(D)J recombination. (amedeo.com)

Four transcription factors (HIC1, p53, E2F1, and cMYC) have been identified to modulate SIRT1 expression under oxidative stress/DNA-damage conditions and/or nutrient deprivation. (biomedcentral.com)

Herein, we show that E2F1 directly transactivates the c-NHEJ core factors Artemis, DNA-PKcs, ligase IV, NHEJ1, Ku70/Ku80 and XRCC4, but indirectly inhibits APLF, a chromatin modifier regulating c-NHEJ. (biomedcentral.com)
XIAP binds and inhibits initiator caspase-9, which is directly involved in the activation of executioner caspase-3. (ac-devd-choinhibitor.com)

Mature miRNA, along with Agonaute 2, forms an RNA-dependent silencing complex and binds to the 3′-UTRs of the target gene mRNAs with imperfect complementarity to cause their degradation or translational suppression [ 11 , 12 ]. (biomedcentral.com)

Fifty four cases were analysed by polymerase chain reaction to detect the t(14;18) translocation and immunohistochemistry for BCL2, CD10, BCL6, and E2F1 expression. (bmj.com)
RB and c-Myc activate expression of the E-cadherin gene in epithelial cells through interaction with transcription factor AP2. (nih.gov)
The first is quantitative control of expression, which is predominantly achieved by transcription. (webflow.io)
Regulating transcription (i.e., the synthesis of an RNA sequence derived from a DNA template) is the fundamental process by which nature controls the early stages of gene expression, and ultimately cellular function. (webflow.io)
It is well-known that epigenetics is strongly modified in tumor cells and that epigenetic regulators influence the expression and function of Cytochrome P450 (CYP) enzymes through altering crucial transcription factors responsible for drug-metabolizing enzymes. (cancerindex.org)

The E2F4 transcription factor is required for both roles and consequently for multiciliogenesis. (molbiolcell.org)
This sequence is highly conserved across the E2F family, but the ability to bind Deup1 and SAS6 is specific to E2F4 and E2F5, consistent with their shared roles in multiciliogenesis. (molbiolcell.org)
By generating E2F4/E2F1 chimeras, we identify a six-residue motif that is critical for Deup1 and SAS6 binding. (molbiolcell.org)

MIR888-dependent inhibition of APLF by E2F1 was demonstrated using overexpression and knockdown experiments, in combination with luciferase assays. (biomedcentral.com)

The factors activate the relative signaling pathways, such as the Ras/Raf/mitogen-extracellular activated protein kinase kinase (MEK)/ extracellular signal-regulated kinase (ERK), phosphatidylinositol-3-kinase (PI3K)/phosphatase and tensin homologue deleted on chromosome ten (PTEN)/Akt/mammalian target of rapamycin (mTOR), that are involved in cancer cell survival via interactions with their specific receptors and then maintain the malignant growth of tumors [ 9 ]. (oncotarget.com)

Single-stranded-DNA binding alters human replication protein A structure and facilitates interaction with DNA-dependent protein kinase. (nih.gov)
Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. (nih.gov)

miRNAs are initially generated as primary transcripts (pri-miRNA) from inter- and intragenic chromosomal regions predominantly via RNA polymerase II mediated transcription, and are then further processed by the Drosha RNase III enzyme to produce short hairpin pre-miRNAs [ 11 ]. (biomedcentral.com)

p27 is a direct inhibitor of cyclin-dependent kinase 2 (cdk2), one of the cdks responsible for the activation of E2F1 transcription factors that promote DNA replication. (medscape.com)
This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. (thermofisher.com)

The sulfation of N-acetylglucosamine residues of heparan sulfate proteoglycans (HSPGs) on cell surface mediates the interaction or binding of growth factors and their receptors [ 10 ], which is a critical link for the cascade activation of signal pathways. (oncotarget.com)
Hence, E2F1 is either inefficient in controlling DSB repair in this setting, or rewires the repair apparatus towards alternative, error-prone DSB processing pathways. (biomedcentral.com)

Eighteen of 54 cases had bcl2 gene rearrangement, 36 of 52 expressed BCL2, 29 of 52 expressed BCL6, 20 of 53 expressed CD10, and 18 of 53 expressed E2F1. (bmj.com)

lncNB1 binds to the ribosomal protein RPL35 to enhance E2F1 protein synthesis, leading to DEPDC1B gene transcription. (ugent.be)
This study therefore identifies lncNB1 and its binding protein RPL35 as key factors for promoting E2F1 protein synthesis, N-Myc protein stability and N-Myc-driven oncogenesis, and as therapeutic targets. (ugent.be)

Design of a synthetic Mdm2-binding mini-protein that activates the p53 response in vivo. (nih.gov)

A central role for the E2F-1 transcription factor in the DNA damage response pathway is gaining support. (ox.ac.uk)
Signaling pathway studies indicated that FGF21 could bind fibroblast growth factor receptor (FGFR) 1/2 and coreceptor -Klotho after being secreted from tissues [ 3 , 4 ]. (hindawi.com)

Fibroblast growth factor 21 (FGF21), a secretion protein, functions as a pivotal regulator of energy metabolism and is being considered as a therapeutic candidate in metabolic syndromes. (hindawi.com)
Fibroblast growth factor 21 (FGF21), a member of the FGF family, was initially identified by Nobuyuki Itoh's group [ 1 ], and its bioactivity was first discovered as a potential regulator of glucose uptake in mouse and human adipocytes [ 2 ]. (hindawi.com)

The protein encoded by this gene is a member of the E2F family of transcription factors. (thermofisher.com)

MYBL2 is a key downstream factor of Akt/FoxM1 signaling to promote progression of human glioma, and could be a new candidate gene for molecular targeting therapy and biomarker for radiotherapy of glioma. (cusabio.com)

Binding experiments show that the spacing of the pSer with respect to L24 is flexible, and that the Pro required for the [S/T]P phosphosite is suboptimal, since a P29A mutation increases binding affinity by 50-fold. (eu.org)

LIG_RB_pABgroove_1 (LxDLFD), LIG_RB_LxCxE_1 (LxCxE) and LIG_RBL1_LxSxE_2 (LxSxE).The LxDLFD motif binds to a deep groove formed between the A and B subdomains and is present in E2F family transcription factors (E2F1-5). (eu.org)

p107-specific interactions other than the phosphate-binding pocket are likely to contribute to LIN52-p107 specificity. (eu.org)

In this context, the AHR, a ligand-activated transcription factor and key regulator in xenobiotic metabolism and immunity, seems to be a promising molecular target. (frontiersin.org)

[ 8 ] The growth factor receptors HER2 and epidermal growth factor receptor (EGFR) activate PI3K, and they both figure prominently as oncogenes in numerous malignancies. (medscape.com)
Upstream effectors of ETS (epidermal growth factor receptor, protein kinases C and D) were also evaluated. (kenstoreylab.com)

However, invasive bladder cancers (BC) display, in contrast to non-invasive stages, genomic instability despite their high E2F1 levels. (biomedcentral.com)

Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)

Specifically highlighted is the biology of androgen receptor (AR), the nuclear receptor transcription factor largely responsible for androgen-stimulated and castrate-recurrent (CR) PCa. (ijbs.com)

The LxCxE and LxSxE motifs bind to a highly conserved cleft in the B subdomain. (eu.org)
We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)

It translocates to the CELL NUCLEUS and binds DNA to stimulate CASPASE-independent CHROMATIN condensation. (lookformedical.com)

Results suggested that the oncogenic transcription factor HIF-2alpha stabilized VHL disease suppressor B-Myb, which is also a transcription factor, by physical interaction. (cusabio.com)

RT-PCR and immunoblotting, in combination with bioinformatics tools were applied to monitor c-NHEJ factors status in high-E2F1-expressing, invasive BC versus low-E2F1-expressing, non-invasive BC. (biomedcentral.com)

Perturbation of the E2F1/miR-888/APLF axis restores c-NHEJ and ameliorates cell invasiveness. (biomedcentral.com)

Consequently, E2F1/miR-888/APLF rewiring is established, generating conditions of APLF scarcity that compromise proper c-NHEJ function. (biomedcentral.com)
Although levels of ETS increased in most organs of hibernators, ETS binding activity to DNA was greatly reduced indicating a growth suppressive function during torpor. (kenstoreylab.com)

The life of a gene product, from transcription to degradation, is controlled by a series of regulatory decisions. (biomedcentral.com)

Further investigations of factors that directly or indirectly impact back-splicing are needed to enrich our understanding of the biogenesis of circRNAs. (biomedcentral.com)
Harnessing endogenous transcription factors directly by small molecules for chemically induced pluripotency inception. (amedeo.com)

Human DNA-binding protein (dbpA) is a member of a Y-box binding protein family containing a cold shock domain. (avivasysbio.com)

The transcription factor E2F1 supports HR-mediated DSB repair and protects genomic stability. (biomedcentral.com)

Mutation of core residues L18, E22 and L24 weakens binding ( Guiley,2015 ). (eu.org)

Anatomy16

Organisms9

Chemicals and Drugs136

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes70

Disciplines and Occupations1

Information Science3

Proteins12

Promoter5

Preferentially2

Differentiation6

Regulates transcription2

Apoptosis4

Antibody4

Inhibit5

Retinoblastoma protein2

Genes14

Phosphorylation3

Promoters2

Recruits1

Proliferation2

Transcriptional4

Regulation5

CMYC1

Inhibits2

Target gene1

Expression5

E2F43

Inhibition1

Mammalian1

Single-stranded-DNA2

Polymerase1

Cyclin2

Pathways2

BCL21

Synthesis2

Vivo1

Pathway2

Fibroblast2

Family of transcription factors1

Progression1

Increases1

Motif1

Specificity1

Molecular1

Epidermal2

Cancers1

Peptide1

Nuclear1

Motifs2

Chromatin1

Interaction1

Versus1

Cell1

Function2

Regulatory1

Directly2

Protein family1

Stability1

Mutation1