• Although these systems aren't known completely, research survey that appearance of cyst encoding protein and genes is normally upregulated, like the Wnt/-catenin pathway functionally. (globaltechbiz.com)
  • This study applies knowledge from structural and evolutionary studies of the rice NLR protein Pik-1 and cognate effector protein AVR-Pik to engineering of new disease resistance genes. (elifesciences.org)
  • Many of the characterized resistance (R) genes used to confer disease resistance in crop breeding programs encode NLR proteins ( Kourelis and van der Hoorn, 2018 ). (elifesciences.org)
  • Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
  • Additionally, we identified mutants regulating the actin cytoskeleton (Rvs161p and Vrp1p), known membrane traffic regulators (Kes1p and Chs5p), and several unknown genes. (ox.ac.uk)
  • In contrast, the Fushi Tarazu homeodomain protein is an activator, both in cultured cells and in Drosophila embryos, where it activates several known target genes, including its own gene. (biologists.com)
  • Normal Fushi Tarazu target genes are affected as they are in fushi tarazu mutants. (biologists.com)
  • The UAG codon sequence prevents any genes encoded after it, like the green fluorescent protein, from being translated, as UAG signals to ribosomes, the cell's protein-synthesizing machines, to stop the process of translating RNA into protein. (harvard.edu)
  • In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. (microbialcell.com)
  • In this respect, although obviously lacking a brain with a multicellular neuronal network, simple unicellular yeast are of great value to investigate the effects of overexpression and single point-mutations in genes encoding key proteins on cell physiology and on the function of conserved signaling cascades. (microbialcell.com)
  • Another special mechanism by which the plant controls the expression of genes in the chloroplast is via the utilization of specific pentatricopeptide repeats (PPR) proteins. (igem.org)
  • Proteins produced from GENES that have acquired MUTATIONS. (bvsalud.org)
  • Two different chimeric antigen receptor (CAR) proteins (yellow and orange globes) extend from the duoCar-T cell's membrane. (einsteinmed.edu)
  • This receptor, called a chimeric antigen receptor (the CAR in CAR-T), was designed specifically to express binders, which can be antibodies or other molecules that specifically recognize proteins expressed by tumor cells. (einsteinmed.edu)
  • There remains an urgent need for the noninvasive tracking of transfused chimeric antigen receptor (CAR) T cells to determine their biodistribution, viability, expansion, and antitumor functionality. (snmjournals.org)
  • Slpr encodes four recognizable domains: Src-homology three (SH3), kinase, leucine zipper (LZ), and Cdc42/Rac interactive binding motif (CRIB), clustered within the N-terminal half of your protein. (esiservizi.com)
  • Tak1 encodes a protein with an N-terminal kinase domain plus a conserved C-terminal domain (CTD) as shown. (esiservizi.com)
  • Splicing of the NS segment, which encodes the host shutoff protein non-structural protein 1 (NS1), generates the nuclear export protein (NEP/NS2) that is involved in viral RNP export from the host nucleus [ 8 ]. (biomedcentral.com)
  • In this thesis, a chimeric JFH1 genome that encodes the p7 protein of genotype (GT) 1b strain J4 was generated. (monash.edu)
  • The fusion transcript encodes a chimeric protein that couples a segment of the heat shock protein, DNAJB1, with the catalytic domain of protein kinase A (PKA) and exhibits full retention of PKA activity. (medscape.com)
  • This technology is dependant on a little recombinant proteins Advertisement35K++ that binds with high affinity to Compact disc46. (biotech-angels.com)
  • The current presence of the label while enabling easy purification by Ni-NTA chromatography gets the potential to improve the immunogenicity from the recombinant proteins. (biotech-angels.com)
  • increased degrees of Compact disc46 may donate to the ineffectiveness of rituximab in treating repeating NHL.19 CD46 can be a receptor for several pathogens including measles virus and binds to recombinant CD46 cross-linking several CD46 molecules. (biotech-angels.com)
  • The recombination breakpoint is located in the receptor-binding domain of spike, and each region of the recombinant spike confers immune evasion and increases fusogenicity. (bvsalud.org)
  • We generated chimeric recombinant SARS-CoV-2 encoding the S gene of Omicron in the backbone of an ancestral SARS-CoV-2 isolate and compared this virus with the naturally circulating Omicron variant. (biorxiv.org)
  • We obtained recombinant MVA expressing the unmodified full-length SARS-CoV-2 spike (S) protein containing the D614G amino-acid substitution (MVA-Sdg) and a version expressing a modified S protein containing amino-acid substitutions designed to stabilize the protein a in a pre-fusion conformation (MVA-Spf). (bvsalud.org)
  • Hence, the novel surrogate myristoyl moiety not only failed to increase NOS2 activity when introduced in a wild-type sequence environment, but was also unable to rescue the inactive phenotype of the Cys3Ser mutant. (biologists.com)
  • When EFE is ubiquitously expressed, it overrides endogenous Fushi Tarazu and causes a fushi tarazu mutant phenotype. (biologists.com)
  • Biopsy protein studies in young boys are thought to provide further evidence to help predict Duchenne versus Becker muscular dystrophy phenotype but that has not been conclusively demonstrated and again, there are a small proportion of outliers. (medscape.com)
  • This attenuated phenotype was restored by M replacement of H3N2 M in a chimeric H1N1 virus, despite low M2 levels. (biomedcentral.com)
  • We demonstrate that the extended recognition profiles of engineered Pikp-1 variants correlate with effector binding in planta and in vitro, and with the gain of new contacts across the effector/HMA interface. (elifesciences.org)
  • We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)
  • Moss KCBP is postulated to transport the nucleus and chloroplast via direct binding to their membranes, since it binds to and transports liposomes composed of phospholipids in vitro . (go.jp)
  • We confirmed a glycan-dependent binding of hemocyanins to chimeric TLR4 in vitro. (exeter.ac.uk)
  • A human blood chimeric mouse could serve to harmonize in vitro P. falciparum cultivation and in vivo studies carried out in rodent animal models. (frontiersin.org)
  • Autophagy receptors are endowed with the ability to both recognize the ubiquitination signals of cargoes by binding the ubiquitin-binding domain (UBD) and interact with the ATG8/LC3/GABARAP protein on the autophagosome membrane, relying on their LC3-binding region (LIR), which is followed by the delivery of cytoplasmic cargo to the incipient autophagosome for engulfment and ultimately lysosomal degradation 20-22 . (researchsquare.com)
  • A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by phytopathogens to promote infection, through unconventional integrated domains which resemble the effector's host targets. (elifesciences.org)
  • These receptors detect effector proteins which are delivered into host cells by invading pathogens and pests to promote virulence. (elifesciences.org)
  • NLR receptors have a modular domain architecture typically consisting of an N-terminal coiled-coil (CC or CC R ) or Toll/Interleukin-1 receptor (TIR) domain, a central nucleotide-binding (NB-ARC) domain, and a C-terminal leucine-rich repeat (LRR) domain. (elifesciences.org)
  • We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR). However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown. (exeter.ac.uk)
  • Chimeric antigen receptors (CARs) endow T cells with antigen-specific recognition, activation, and proliferation independent of major histocompatibility complex ( 1 - 3 ). (snmjournals.org)
  • CR3022 has been shown to bind with a high affinity to SARS-CoV2 (32416259, 32413276). (abcam.com)
  • Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. (wikidoc.org)
  • Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. (bvsalud.org)
  • Our results showed that individual mutations of Val 19 to Glu and Val 34 to Asp in the EGF domain of PE(ΔIa)-EGF mutants resulted in an increase in the binding affinity to EGF receptor and cytotoxicity to A431 cells. (tmu.edu.tw)
  • On the other hand, individual mutations of His 16 to Asp and Gly 18 to Ala in the EGF domain of PE(ΔIa)-EGF mutants lead to a decrease in the binding affinity to EGF receptor and cytotoxicity to A431 cells. (tmu.edu.tw)
  • The compound showing the highest binding affinity to the viral S protein was further characterized by surface plasmon resonance (SPR) towards ACE2 and viral S protein binding domain. (bvsalud.org)
  • E ) IP of FLAG-HDAC4 deletion mutants coexpressed with Myc-CaMKIIδB-T287D to identify a domain of HDAC4 that binds to activated CaMKIIδB. (jci.org)
  • We expressed a GFP-tagged plasma membrane protein in the yeast deletion library and identified mutants with altered marker localization. (ox.ac.uk)
  • Deletion of HVR1 increases CD81-binding ability of the mutant and also increases its susceptibility to neutralizing antibody MAb 24. (monash.edu)
  • Twenty five tests were carried out on mutant mice and two significant abnormalities were observed. (wikipedia.org)
  • In initial studies utilizing a 6 × histidinyl tagged proteins we had proven that intravenous Advertisement35K++ injection in conjunction with rituximab was secure and improved rituximab-mediated eliminating of Compact disc20-positive focus on cells in YYA-021 mice and non-human primates (NHPs). (biotech-angels.com)
  • In today's research we performed preclinical research in two pet varieties (mice and NHPs) with this proteins demonstrating its protection and efficacy. (biotech-angels.com)
  • Active immunization with fibrillar beta-amyloid peptide (AB42) as well as passive transfer of anti-AB antibodies significantly reduces AB plaque deposition, neuritic dystrophy, and astrogliosis in the brain of mutant amyloid precursor protein (APP)-transgenic mice. (ecu.edu)
  • also found that the oocytes of mice already contain all the proteins necessary to activate p63. (elifesciences.org)
  • Dr. Dowd investigates the role of the bone protein osteocalcin on glucose homeostasis, insulin sensitivity, secretion, bone properties and bone fracture using osteocalcin knockout mice (KO). (cuny.edu)
  • huRBC-reconstituted immunodeficient mice received infectious challenge with attenuated P. falciparum C9 parasite mutants (C9-M), complemented (C9-C), and wild type (NF54) progenitors to study the role of immune effectors in the clearance of the parasite from mouse circulation. (frontiersin.org)
  • Collectively, our data suggest that huRBCs reconstituted NSG mice infected with attenuated P . falciparum is a valuable tool to explore the role of C9 mutation in the growth and survival of parasite mutants and their response to the host's immune responses. (frontiersin.org)
  • To explore the physiological role of the various levels of M2 protein in pathogenicity, we challenged C57BL/6 mice with the H1N1 WSN wild-type strain, mutant H1N1 (55T), and chimeric viruses including H1N1 + H3wt and H1N1 + H3mut. (biomedcentral.com)
  • However, the domesticated transposase domain retains its ability to bind to the mariner repeat elements in the genome. (wikipedia.org)
  • This visual screening method can now be used for different cargo proteins to search in a genome-wide fashion for machinery involved in post-Golgi sorting. (ox.ac.uk)
  • Expression from the human cytomegalovirus major IE promoter, when cloned into the genome of HSV-1 mutants, was also increased by inhibition of protein synthesis. (microbiologyresearch.org)
  • The intergenotypic chimeric genome was nonviable in human hepatoma cells and infectious chimeric virions were only produced after cells harboring the chimeric genomes were passaged several times. (monash.edu)
  • [ 19 ] The apparent lack of a second-hit mutation in the genome of FLCs supports the role of DNAJB1-PRACA fusion protein as a major driver of this tumor and as a key diagnostic and therapeutic target. (medscape.com)
  • These peptides can bind specific RNA sequences and trim pre-mRNA. (igem.org)
  • This study indicates that the cytotoxicity of PE(ΔIa)-EGF mutant to EGF receptor-bearing cells may be used as an indicator to screen mutations of EGF important in EGF-receptor interactions. (tmu.edu.tw)
  • 2001). This area could contribute to Tak1 localization or protein interactions with signaling partners, as recommended by cell culture and biochemical assays (Takaesu et al. (esiservizi.com)
  • The work is of interest to colleagues in synthetic biology, protein engineering and plant-pathogen interactions. (elifesciences.org)
  • We developed a new single-molecule method to probe non-specific DNA interactions for two HMGB homologs: the human HMGB2 box A domain and yeast Nhp6Ap, along with chimeric mutants replacin. (shengsci.com)
  • Protein tyrosine (pTyr) phosphorylation is a common post-translational modification which can create novel recognition motifs for protein interactions and cellular localisation, affect protein stability, and regulate enzyme activity. (embl.de)
  • Using this approach, the functional interactions of p7 and E2 with other viral proteins during HCV replication was investigated. (monash.edu)
  • In summary, this thesis demonstrates the importance of functional interactions between HCV proteins during virus morphogenesis and infectivity. (monash.edu)
  • SARS-CoV-2 binding and entry in human cells involves interactions of its spike (S) protein with several host cell surface factors, including heparan sulfate proteoglycans (HSPGs), transmembrane protease serine 2 (TMPRSS2), and angiotensin-converting enzyme 2 (ACE2). (bvsalud.org)
  • However, the transcriptional activity of TBP (TATA box-binding protein) and its promoter binding factor (TBPF), Calcitriol (Rocaltrol) RNA polymerase II, remain unaffected during differentiation. (globaltechbiz.com)
  • Here we show that the maize dominant Barren inflorescence3 ( Bif3 ) mutant harbors a tandem duplicated copy of the ZmWUS1 gene, ZmWUS1-B , whose novel promoter enhances transcription in a ring-like pattern. (nature.com)
  • In this study, by characterization of the dominant maize mutant Barren inflorescence3 ( Bif3 ) we identified a tandem-duplicated copy of ZmWUS1 whose expression is dramatically enhanced by the insertion of a short stretch of chimeric proximal promoter sequence. (nature.com)
  • The Nkx-dependent promoter will include the Nkx2-5 binding site 5′-TNAAGTG-3′, as well as a minimal promoter such as Cmv or SV40. (regensci.org)
  • The Lambda Activator bind to the Lamda-vp16 Operator in the Minimal Promoter. (regensci.org)
  • Activation of the ICP0 promoter was observed when protein synthesis was blocked by alternative agents. (microbiologyresearch.org)
  • In addition, mutations of any of the cysteine residues of EGF in PE(ΔIa)-EGF mutants resulted in the loss of their binding activity to EGF receptor and a corresponding loss of their cytotoxicity. (tmu.edu.tw)
  • These variants convergently acquired amino acid substitutions at critical residues in the spike protein, including residues R346, K444, L452, N460, and F486. (bvsalud.org)
  • Those which bind phosphorylated tyrosine residues may recruit multi-phosphorylated substrates for the adjacent active domains and are more conserved, while the other class have accumulated several variable amino acid substitutions and have a complete loss of tyrosine binding capability. (embl.de)
  • The Carboxy Terminal Residues 220-283 Are Not Required for Voltage Gating of a Chimeric Connexin 32 Hemichannel. (cuny.edu)
  • The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
  • 2016) "Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy. (cuny.edu)
  • The NRs specifically bind mutp53 in tumor cells via mutp53-binding peptide (MBP). (researchsquare.com)
  • M1 is a structural matrix protein that stabilizes viral particles, whereas the ion channel protein M2 is embedded in the viral envelope and regulates the uncoating of vRNP [ 9 ]. (biomedcentral.com)
  • RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
  • A small protein of 63 amino acids, p7 is encoded at the junction of the structural and non-strucutural region. (monash.edu)
  • ADARs are enzymes that bind to double-stranded RNA (dsRNA) molecules and make a specific base edit, converting a mismatched adenosine (A) molecule into inosine (I). This change destabilizes the dsRNA structure, and is thought to be involved in cells' responses to different viruses, many of which carry their genetic material as RNA. (harvard.edu)
  • Next, we determined the effects of single nucleotide variations on M splicing by generating mutant viruses harboring the 55C/T variant through reverse genetics. (biomedcentral.com)
  • To investigate the emergence of compensatory mutations in the viral proteins during cell passaging, the consensus sequences of the entire polyprotein coding regions of the wild type JFH1 and three chimeric viruses were determined. (monash.edu)
  • Transcriptome analysis of the placenta showed that gal-1, gal-3, and several PSG and glycoenzymes responsible for the synthesis of gal-1-binding glycotopes (such as linkage-specific N-acetyl-glucosaminyltransferases (MGATs)) are upregulated in pregnant women infected with SARS-CoV-2. (bvsalud.org)
  • Cells continuously monitor protein synthesis to prevent accumulation of aberrant polypeptides. (shengsci.com)
  • The PI3K/Akt/mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. (go.jp)
  • This screen revealed a requirement of several enzymes regulating the synthesis of sphingolipids and ergosterol in the correct and efficient delivery of the marker protein to the cell surface. (ox.ac.uk)
  • Herpes simplex virus type 1 (HSV-1) transcription can be arrested at the immediate early (IE) stage by continuous treatment of cells with inhibitors of protein synthesis, usually cycloheximide, from the time of infection. (microbiologyresearch.org)
  • Expression from the HSV-1 IE promoters that control synthesis of ICP0 and ICP27 was, unexpectedly, stimulated by inhibition of protein synthesis. (microbiologyresearch.org)
  • Since inhibitors of protein synthesis are known to activate cellular signal transduction pathways, our findings demonstrate new mechanisms for the regulation of HSV-1 IE gene expression which may be important during latency and reactivation. (microbiologyresearch.org)
  • Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. (microbiologyresearch.org)
  • Protein synthesis inhibitors differentially superinduce c-fos and c-jun by three distinct mechanisms: lack of evidence for labile repressors. (microbiologyresearch.org)
  • Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis. (microbiologyresearch.org)
  • Effect of protein synthesis inhibitors on growth factor activation of c- fos , c- myc , and actin gene transcription. (microbiologyresearch.org)
  • Reverse genetic analysis demonstrated that any one of the single mutations restored the infectivity of the defective chimeric genomes. (monash.edu)
  • Unless HIV is actively replicating, its tell-tale viral proteins don't appear on the surface of T cells, so the latent virus in its host cell remains invisible to the immune system or to pharmacologic attack. (einsteinmed.edu)
  • The recently developed HCV cell culture system, based on the JFH1 strain of HCV, has provided an opportunity to study the role of the viral proteins in the complete HCV replication cycle in human hepatoma cells. (monash.edu)
  • How the viral proteins functionally interact during replication of HCV in cell culture is not completely understood. (monash.edu)
  • After binding to gp120, the CAR proteins use their signaling pathways (yellow and orange cylinders) to activate the duoCar-T cell to attack the HIV-infected T cell. (einsteinmed.edu)
  • We are engineering embryonic stem-like cells to autonomously differentiate into cardiomyocytes, recognize and bind to damaged heart cells and then activate a defined program. (regensci.org)
  • Similarly, the expression of other proteins such as the protein disulphide isomerase and cytoskeletal proteins (tubulin, myosin, actin, extendin, and ubiquitin) also remain constant [37,38]. (globaltechbiz.com)
  • Cyclic AMP exhibits its mechanism via protein kinase mediated system. (globaltechbiz.com)
  • Here we show that calcium/calmodulin-dependent kinase II (CaMKII) signals specifically to HDAC4 by binding to a unique docking site that is absent in other class IIa HDACs. (jci.org)
  • Acute leukemias induced by MLL chimeric oncoproteins are among the subset of cancers distinguished by a paradoxical dependence on GSK-3 kinase activity for sustained proliferation. (stanford.edu)
  • 2010). The C terminus of your Tak1 protein harbors a putative regulatory domain CDK7 Inhibitor drug identifiable by an island of sequence conservation among homologs (Takatsu et al. (esiservizi.com)
  • The miniF plasmid C protein: sequence, purification and DNA binding. (shengsci.com)
  • The authors nicely demonstrate that it is indeed possible to engineer resistance proteins with broad recognition specificity for the rice blast fungus. (elifesciences.org)
  • We are investigating how Pbx and Meis proteins contribute to the specificity of Hox function in development and how disruption of their activities leads to neoplasia. (stanford.edu)
  • The Trithorax group (TrxG) is composed of diverse, evolutionary conserved proteins that form chromatin-associated complexes accounting for epigenetic transcriptional memory. (silverchair.com)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • The Engrailed homeodomain protein is an 'active' or dominant transcriptional repressor in cultured cells. (biologists.com)
  • This resembles the β-amyloid precursor protein (APP) in Alzheimer disease (AD), which can be physiologically processed by α-, β-, and γ-secretases. (mdpi.com)
  • This chimeric toxin designated as PE(ΔIa)-EGF kills A431 cells through the EGF receptor-mediated pathway. (tmu.edu.tw)
  • We have constructed 14 different PE(ΔIa)-EGF mutants , and examined their EGF receptor binding activity as well as their cytotoxicity to A431 cells. (tmu.edu.tw)
  • A ) Chimeric HDAC4/5 proteins (as indicated) were expressed in COS cells in the presence of CaMKIIδB-T287D. (jci.org)
  • B ) An HDAC5 mutant, in which the CaMKII consensus sites were mutated to the corresponding sites in HDAC4, was expressed in COS cells alone or with CaMKI c.a. and CaMKIIδB-T287D. (jci.org)
  • The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. (shengsci.com)
  • For instance, how can cells control the diverse patterns of glycosylation of various secretory proteins in the endoplasmic reticulum and Golgi in an orderly manner with high accuracy? (go.jp)
  • The Omicron S-bearing virus robustly escapes vaccine-induced humoral immunity, mainly due to mutations in the receptor-binding motif (RBM), yet unlike naturally occurring Omicron, efficiently replicates in cell lines and primary-like distal lung cells. (biorxiv.org)
  • HIV needs CCR5 to bind to and enter a T cell, so T cells with mutated CCR5 are highly resistant to HIV infection. (einsteinmed.edu)
  • The Berlin patient's success got us and other investigators thinking, 'What if we could duplicate that strategy of treating with HIV-resistant T cells, but using CAR-T cells instead of a stem cell transplant from a CCR5 mutant donor? (einsteinmed.edu)
  • Each protein recognizes a different part of gp120 (green balls), the HIV glycoprotein expressed on the surface of HIV-infected T cells. (einsteinmed.edu)
  • Bound cells will express anti-apoptosis and anti-necrosis proteins to prevent cell death, express increased amounts of a cell-cell adhesion molecule to stimulate the formation of a sheet of cells and to cause some of the cells to penetrate into the inner layers of the heart. (regensci.org)
  • Cells that fail to bind in a defined amount of time are programmed to self-destruct. (regensci.org)
  • DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
  • Characterization of the Zn(II) binding properties of the human Wilms' tumor suppressor protein C-terminal zinc finger peptide. (cuny.edu)
  • Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. (microbiologyresearch.org)
  • Construction and characterization of herpes simplex virus type 1 mutants with defined lesions in immediate early gene 1. (microbiologyresearch.org)
  • We have analysed the effect of cycloheximide on IE gene expression with HSV-1 mutants deficient in the production of functional levels of the three major transactivators, the virion protein (VP16) and two IE proteins (ICP0 and ICP4). (microbiologyresearch.org)
  • In this study, we used a random mutagenesis approach to make point mutations on EGF, followed by replacing the wild type EGF in PE(ΔIa)-EGF with these EGF mutants. (tmu.edu.tw)
  • More than half of human malignant tumors harbor TP53 gene mutations, most of which are point mutations within the DNA-binding domain of TP53, resulting in mutant p53 (mutp53) protein stabilization and accumulation in the cell and enhanced tumor progression. (researchsquare.com)
  • The Omicron spike (S) protein, with an unusually large number of mutations, is considered the major driver of these phenotypes 3 , 8 . (biorxiv.org)
  • Rituximab can be a mouse/human being chimeric monoclonal antibody focusing on Compact disc20 and induces cell eliminating through complement-dependent cytotoxicity (CDC) antibody-dependent cell cytotoxicity antibody-dependent cell-mediated phagocytosis and immediate cell apoptosis.2-6 It really is effective as an individual agent and in conjunction with chemotherapy in the YYA-021 treating Compact disc20-postive B-cell YYA-021 malignancies. (biotech-angels.com)
  • Bound Lu-Fc was detected with an antibody against human IgG as described above. (bio-rad-antibodies.com)
  • This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. (abcam.com)
  • The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. (abcam.com)
  • Precisely, this antibody binds to the 'open' conformation of the spike protein to the amino acids 318-510 in the S1 domain of the SARS-CoV as well as SARS-CoV-2 strains (32245784, Joyce et al. (abcam.com)
  • and Dimiter S. Dimitrov, Ph.D., an expert in antibody and protein design at the University of Pittsburgh. (einsteinmed.edu)
  • DOTA antibody reporter 1 (DAbR1) comprises a single-chain fragment of the antilanthanoid-DOTA antibody 2D12.5/G54C fused to the human CD4-transmembrane domain and binds irreversibly to lanthanoid ( S )-2-(4-acrylamidobenzyl)-DOTA (AABD). (snmjournals.org)
  • C protein binds strongly to pifO, and weakly to sequences in the ori-1 region. (shengsci.com)
  • Therefore, the knockout mouse is not for SETMAR but only the SET domain of this chimeric fusion protein. (wikipedia.org)
  • Lu-Fc only bound to the basement membranes containing α5, primarily neuromuscular junctions. (bio-rad-antibodies.com)
  • In fundamental cell biological processes such as signal transduction, enzymatic activity or intracellular fusion during vesicular transport, a large number of proteins are recruited to and released from the cytoplasmic surface of intracellular membranes (Casey et al. (biologists.com)
  • Deep sequencing of RNA molecules cross-linked to immunoprecipitated protein particles (CLIP-seq) revealed. (shengsci.com)
  • Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartment. (shengsci.com)
  • In 2014, Honeyman and colleagues discovered a novel, chimeric transcript that is present in all studied samples of FLC, DNAJB1-PRKACA . (medscape.com)
  • E2 has a receptor binding domain (E2661RBD) that conatins three variable regions, hypervariable regions 1 (HVR1), HVR2 and the intergenotypic variable region (igVR). (monash.edu)
  • S. aureus is known to bind multiple coagulation factors, resulting in the formation of aggregates and blood clots that might protect the bacterium from the phages. (bvsalud.org)
  • We found that the FERM domain in the tail region, which is known to bind to lipids as well as other proteins, is essential for both nuclear and chloroplast positioning, whereas the proximal MyTH4 domain plays a supporting role in chloroplast transport. (go.jp)
  • Epidermal growth factor (EGF) was fused to the carboxyl end of a modified pseudomonas exotoxin A that has its toxin binding domain deleted. (tmu.edu.tw)
  • Stronach, A. L. Lennox, and R. A. GarlenaFigure 1 Slpr and Tak1 domain organization and derived mutant or chimeric constructs. (esiservizi.com)
  • Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. (bio-rad-antibodies.com)
  • The rice NLR receptor Pik-1 binds the Magnaporthe oryzae effector AVR-Pik through an integrated heavy metal-associated (HMA) domain. (elifesciences.org)
  • This entry represents the PTPase domain found in several tyrosine-specific protein phosphatases (PTPases). (embl.de)
  • Taxonomic distribution of proteins containing PTPc domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with PTPc domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing PTPc domain in the selected taxonomic class. (embl.de)
  • A derivative that lacks this domain, while it cannot repress the endogenous fushi tarazu gene, can still reduce the activity of the fushi tarazu autoregulatory enhancer, suggesting that it competes with endogenous Fushi Tarazu for binding sites in vivo. (biologists.com)
  • We combined Fushi Tarazu targeting and Engrailed active repression in a chimeric regulator, EFE. (biologists.com)
  • In contrast to the wild-type p53 protein, missense mutant p53 (mutp53) proteins are stabilized and accumulate to high levels in the cell 11 . (researchsquare.com)
  • The protein then switches to an active form when DNA damage is detected to trigger the process of cell self-destruction. (elifesciences.org)
  • Taking advantage of an enzyme that edits RNA in the human body, DART VADAR allows researchers to easily design RNA circuits carrying protein-coding information that are only translated in the presence of a specific molecular marker of disease and/or cell type. (harvard.edu)
  • However, if the sensor binds to a complementary target RNA strand in the cell, it becomes a double-stranded RNA molecule. (harvard.edu)
  • While one batch of cell free extract generation might be perfectly suited for efficient protein production, other batches might not perform that well. (igem.org)
  • Lu/B-CAM binding assay on tissue sections. (bio-rad-antibodies.com)
  • After preincubation with anti-Lu/B-CAM antibodies, Lu-Fc was used in the binding assay on tissue sections. (bio-rad-antibodies.com)
  • The DNA minigeneinduced anti-AB antibodies bound to AB plaques in brain tissue from an Alzheimer's disease patient demonstrating functional activity of the antibodies and the potential for therapeutic efficacy. (ecu.edu)
  • Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
  • Consequently, maintaining an appropriate level of protein tyrosine phosphorylation is essential for many cellular functions. (embl.de)
  • Macroautophagy (hereafter referred to as autophagy), an independent protein degradation pathway, involves the phagocytosis of long cyclins and protein aggregates in the cytoplasm into autophagosomes for subsequent lysosomal degradation 17-19 . (researchsquare.com)
  • Only HDAC4 binds strongly to CaMKII. (jci.org)
  • As the mammalian cyclase OAS1 also binds viral double-stranded RNA during the interferon response, our results reveal a conserved mechanism for the activation of innate antiviral defence pathways. (bvsalud.org)
  • C) Inhibitory effects of anti-Lu/B-CAM antibodies on Lu-Fc binding to laminin α5. (bio-rad-antibodies.com)
  • Presence of both antibodies delivers a blocking action of the SARS-COV RBD-ACE2 interaction, by binding two distinct and functional epitopes (16796401). (abcam.com)
  • The binding epitope of clone CR3022 does not overlap with the ACE2 binding site of SARS-COV2 (32065055). (abcam.com)
  • In this paper we investigated the potential of sulphated Hyaluronic Acid (sHA), a HSPG mimicking polymer, to inhibit the binding of SARS-CoV-2 S protein to human ACE2 receptor. (bvsalud.org)
  • The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. (abcam.com)
  • Each segment is wrapped by a nucleoprotein (NP) into a viral ribonucleoprotein (RNP) complex along with a viral RNA polymerase complex comprising polymerase basic 1 (PB1) polymerase, cap‐binding PB2 subunit, and polymerase acidic (PA) endonuclease. (biomedcentral.com)
  • First identified in Drosophila, the conserved Polycomb group (Pc-G) protein complexes were shown to be both writers (Polycomb Repressive Complex 2, PRC2) and readers (Polycomb Repressive Complex 1, PRC1) of H3K27me3 [ 19 , 20 , 21 ]. (biomedcentral.com)
  • Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility. (shengsci.com)
  • By doing this, the protein protects the maturing RNA from degradation from endonucleases found in the chloroplast. (igem.org)
  • Thus, the green fluorescent protein is produced, signaling that the sensor has found and bound to its target. (harvard.edu)
  • brpf1 mutants display anterior transformations of pharyngeal arches due to progressive loss of anterior Hox gene expression. (silverchair.com)
  • The late entry into the S/M phase coincides with the timing for the peak expression of PF13_0027, suggesting that the deficiency in the mutant cycle can be correlated with the gene expression pattern ( 26 ). (frontiersin.org)
  • Galectins, a family of ß-galactoside-binding proteins, modulate immune and inflammatory processes and have been recognized as critical factors in reproductive orchestration, including maternal immune adaptation in pregnancy. (bvsalud.org)
  • Further, the presence of mutant parasites in deep-seated tissues suggests the escape of parasites from the host's immune responses and thus extended the survival of the parasite. (frontiersin.org)
  • These mutants not only lead to cancer progression due to loss of wild-type p53 activity but also enhanced oncogenic functions (gain-of-function (GOF)), including enhanced tumorigenesis, invasion, metastasis and resistance to therapeutic drugs 12, 13 . (researchsquare.com)
  • In the absence of palmitoylation, introduction of a surrogate myristoylation site resulted in a mutant NOS2 with only 25% activity compared with the wild-type enzyme. (biologists.com)
  • Among them, inducing mutp53 degradation is the most straightforward strategy for disease treatment in eukaryotes, but proteasomes do not efficiently degrade certain large proteins or aggregates due to the inability of these factors to enter the narrow cavity of the proteasome 16 . (researchsquare.com)