Bind lipids and proteins in membranes. Medical search. Frequent questions

These molecules enabled us to address the question, where the Gb 3 sphingolipids are localized prior protein binding by means of fluorescence microscopy on giant unilamellar vesicles. (springer.com)
No evidence for binding to membranes or lipid vesicles by TbMORN1 either in vivo or in vitro could be obtained. (biorxiv.org)
Curvature-mediated assembly of Janus nanoparticles on membrane vesicles. (mpg.de)
The cells actively release extracellular vesicles (EVs), nano-sized (.05-1 μm) membrane-bound vesicles, into the maternal and the fetal circulation. (rochester.edu)
Previous studies have shown that the enzyme is heavily accumulated in the cell's Golgi apparatus, which is the organelle that packages new protein into vesicles before sending them off to the cell's membrane. (epfl.ch)
Extracellular vesicles (EVs) are lipid membranous vesicles released from almost all cell types, and they provide a tremendous opportunity as sources of novel biomarkers from liquid biopsies, as well as agents for tissue repair and wound healing in regenerative medicine. (selectbiosciences.com)
We use plasma extracellular vesicle (EV) protein content of vesicles from plasma sub-fractions on plasma of stroke and peripheral artery disease(PAD) patients, patients after carotid atherectomy (CEA) and patients suspected for chronic coronary syndrome (CCS). (selectbiosciences.com)
Here, cryo-EM is used to examine the protein-lipid architecture of this membrane in tubular vesicles obtained from the (muscle-derived) electric organ of the Torpedo ray. (iucr.org)
As reported earlier, the helical arrangement of the protein component of the vesicles facilitates image averaging and enables us to determine how cholesterol and phospho-lipid molecules are distributed in the surrounding matrix, using headgroup size as a means to discriminate between the two kinds of lipid. (iucr.org)
Here, we analyse by cryo-EM the architecture of this membrane in vesicles isolated from the (muscle-derived) electric organ of the Torpedo ray, with view to defining the protein-lipid interplay required to achieve an optimal neurotransmitter response. (iucr.org)
Nearly all cells secrete membrane-bound vesicles (extracellular vesicles, EVs) that have the capacity to transmit protein, nucleic acid, and lipid signaling mediators between cells. (cdc.gov)
We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)

Combining X-ray crystallography and molecular dynamics simulations, they showed that APTs contain in their structure positively-charged patches that allow them to electrostatically attract the lipid bilayer of a membrane. (epfl.ch)
It is shown that cholesterol segregates preferentially around the receptors in both leaflets of the lipid bilayer, interacting robustly with specific transmembrane sites and creating a network of bridging microdomains. (iucr.org)
A simplified model membrane system called a supported lipid bilayer (SLB) is often used to mimic the membrane of the APC. (lu.se)
A combination of fluorescence microscopy, neutron reflectometry, and time-of-flight secondary-ion mass spectrometry data will be presented which characterizes the structure and composition of this new supported lipid bilayer category. (lu.se)
Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes. (lu.se)

The majority of signal transduction pathways involve the binding of signaling molecules, known as ligands, to receptors that trigger events inside the cell. (wikipedia.org)
Most ligands are soluble molecules from the extracellular medium which bind to cell surface receptors. (wikipedia.org)
In addition, some molecules such as steroid hormones are lipid-soluble and thus cross the plasma membrane to reach cytoplasmic or nuclear receptors. (wikipedia.org)
Antibodies can label immature or inactive proteins hence their usefulness to quantify functionally relevant pools of signaling molecules is also limited. (nature.com)
Small molecules readily penetrate tissue preparations and bind their targets with known stoichiometry. (nature.com)
During digestion, large biological molecules are hydrolysed to smaller molecules that can be absorbed across cell membranes. (aqa.org.uk)
Either stereo reactants or stereo catalysis from achiral or chiral molecules are a prerequisite to obtain pure enantiomeric lipid derivatives. (mdpi.com)
With these molecules in hand, it became possible to investigate the phase behaviour of liquid ordered/liquid disordered supported membranes doped with the Gb 3 species by means of fluorescence and atomic force microscopy. (springer.com)
In contrast, globosides are neutral lipid molecules. (springer.com)
EVs shuttle cargoes of bioactive molecules, such as proteins, lipids, and nucleic acids, from trophoblasts to the recipient cells, modifying gene expression and biology in the cells. (rochester.edu)
The BAR domain of PICK1 binds to lipid molecules, mainly phosphoinositides. (karger.com)
The site allows the enzyme to be bound by ubiquitin, a protein that the cell uses to mark molecules for breakdown. (epfl.ch)
When membrane proteins are crystallised and X-ray crystallography is used to determine their 3D structure, non protein molecules can often be seen surrounding their hydrophobic regions. (ukri.org)
These molecules could either be lipids or detergent molecules, that were used to isolate the proteins from their membranes. (ukri.org)
These have been successfully used to visualize these molecules at the surface of two different membrane proteins, reaction centres and light harvesting complexes by X-ray crystallography. (ukri.org)
These studies have unequivocally allow lipids and detergent molecules to be visualized and have shown the their binding sites at the surface of these proteins are promiscuous. (ukri.org)
ABC (ATP binding cassette) transporters catalyze the transport of a wide variety of molecules across lipid bilayers into or out of cells and organelles. (rug.nl)
A low level of signaling was also obtained when functionalizing the blocked SLBs with adhesion molecules binding to receptors on the T cell. (lu.se)
To better understand how, and if, adhesion molecules of different lengths influences TCR-pMHC binding the 2D Kd of TCR-pMHC in the presence of differently-sized adhesion molecules was studied. (lu.se)
Furthermore, even for height-matched adhesion molecules the 2D Kd of the TCR-pMHC interaction was found to be dependent on the relative density fraction of TCR to adhesion molecules, with low fractions of TCR molecules giving 2-3 times weaker binding. (lu.se)

Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
Lysosomes are membrane-bound organelles containing digestive enzymes that can break down proteins, lipids, carbohydrates and nucleic acids. (cellimagelibrary.org)
Therefore, researchers have tried injecting exosomes -- membrane-bound sacs containing proteins, lipids and nucleic acids secreted by stem cells -- into the heart, but they often break down before they can have therapeutic effects. (scienceblog.com)

Strong interactions between lipids and proteins occur primarily through association of charged headgroups and amino acid side chains, rendering the protonation status of both partners important. (ox.ac.uk)
Specific protein-lipid interactions are critical for viral assembly. (elifesciences.org)
The matrix (MA) domain drives Gag onto the plasma membrane through electrostatic interactions at its highly-basic-region (HBR), located near the myristoylated (Myr) N-terminus of the protein. (elifesciences.org)
Our realistic membrane models confirm interactions with PIP 2 and PS lipids are highly favored around the HBR, and are strong enough to keep the protein bound even without Myr insertion. (elifesciences.org)
The research for my thesis focused on studying the interactions between nanoparticles and cellular membranes in an attempt to understand potentially toxic effects of nanoparticles. (columbiabasin.edu)
The structural plasticity of the ligand binding cavity and the predominant involvement of non-specific van der Waals interactions with the hydrophobic tail of the ligands provide a structural explanation for the non-specificity of maize nsLTP. (rcsb.org)
The researchers believe that these molecular interactions can explain why so many viruses make holes in their target membranes, why the fusion machinery of influenza variants typically does not mutate, and how spike proteins allow viral genetic material to enter the cell. (nih.gov)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
The comparatively few differences between the two rim samples likely reflect specific protein-lipid interactions. (nih.gov)
Interplay of trans- and cis-interactions of glycolipids in membrane adhesion. (mpg.de)
Weak carbohydrate-carbohydrate interactions in membrane adhesion are fuzzy and generic. (mpg.de)
Application areas span many topics ranging from electrochemical energy storage, charge and energy transport in correlated materials, light-matter interactions, and how non-covalent interactions influence drug binding, biomolecular structure, and crystal packing. (gatech.edu)
My current research will be on interactions of membrane proteins and lipids in bacteria. (warwick.ac.uk)
A trehalose-functionalized photoaffinity nanoprobe that could photo-crosslink the protein receptor on mycobacteria was developed to investigate these specific interactions further. (uml.edu)
The team also uncovered a mildly hydrophobic loop on the surface of APTs that they called the "β tongue", which allows the enzyme to perform hydrophobic interactions with the membrane. (epfl.ch)
First, long-range electrostatic interactions attract the enzyme, through its positive patches, to the lipid membrane. (epfl.ch)
In many cases these interactions are actually essential for the protein to function correctly. (ukri.org)
Cholesterol interactions with the receptor are apparently essential for stabilizing and maintaining its physiological architecture, since the transmembrane structure contracts, involving displacements of the helices at the outer membrane surface by ∼2 Å (1-3 Å), when this lipid is extracted. (iucr.org)
One of the ABC transporters that is studied in detail is the osmoregulatory ABC transporter OpuA (class D). Osmotic control of OpuA involves gating by intracellular ionic strength and is mediated by lipid-protein interactions. (rug.nl)

Coarse-grained molecular dynamics simulations, in mixed lipid bilayers, further implicate changes in charge by demonstrating preferential binding of anionic POPG over zwitterionic POPC to protonated OmpF, an effect not observed to the same extent for AmtB. (ox.ac.uk)
The products of the hydrolysis cannot form bilayers leading to a change in membrane conformation and ultimately to a block in the release of neurotransmitters. (nih.gov)
Headgroup Structure and Cation Binding in Phosphatidylserine Lipid Bilayers. (mpg.de)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)

Endoplasmic reticulum and Golgi apparatus membranes,cytoplasmic vesicle. (novusbio.com)
We investigate the mobility of proteins in the bacterial cytoplasm and cytoplasmic membrane as well as membrane model systems, using optical microscopy-based techniques such as fluorescence correlation spectroscopy (FCS) and pulsed-FRAP (panel a). (rug.nl)

We have overcome this by using SMA to coimmunopurify membrane proteins and their native lipids from two regions of photoreceptor ROS disks. (nih.gov)
Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

Proteins responsible for detecting stimuli are generally termed receptors, although in some cases the term sensor is used. (wikipedia.org)
Components of the extracellular matrix such as fibronectin and hyaluronan can also bind to such receptors (integrins and CD44, respectively). (wikipedia.org)
In the case of steroid hormone receptors, their stimulation leads to binding to the promoter region of steroid-responsive genes. (wikipedia.org)
Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D 3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. (nature.com)
secreted PLA2s have also been found to specifically bind to a variety of soluble and membrane proteins in mammals, including receptors. (nih.gov)
Specifically, the mechanism of action of corticosteroids is believed to begin with diffusion across the cell membrane and subsequent binding to glucocorticoid receptors residing in the cytoplasm of target cells 5 . (ersjournals.com)

We find that binding of anionic phosphatidylglycerol (POPG) or zwitterionic phosphatidylcholine (POPC) to OmpF is sensitive to electrospray polarity while the effects of charge are less pronounced for other proteins in outer or mitochondrial membranes: the ferripyoverdine receptor (FpvA) or the voltage-dependent anion channel (VDAC). (ox.ac.uk)
The changes elicited by ligand binding (or signal sensing) in a receptor give rise to a biochemical cascade, which is a chain of biochemical events known as a signaling pathway. (wikipedia.org)
The binding of a signaling molecule with a receptor causes a change in the conformation of the receptor, known as receptor activation. (wikipedia.org)
The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
2018 ). Here, we will solely focus on the less recognized globosides and in particular the globoside Gb 3 , which is the membrane receptor for vero toxins. (springer.com)
Membrane fluctuations and acidosis regulate cooperative binding of "marker of self" CD47 with macrophage checkpoint receptor SIRPα. (mpg.de)
We further compared neutralization capacity of vaccination-induced IgG against 4 SARS-CoV-2 variants of concern (Alpha, Beta, Gamma, and Delta) by angiotensin-converting enzyme 2 receptor-binding domain competition assay. (cdc.gov)
Important pharmacokinetic and pharmacodynamic characteristics that can enhance the efficacy of ICS include small particle size, high glucocorticoid-receptor-binding affinity, long pulmonary residence time and lipid conjugation. (ersjournals.com)
This binding results in an activated glucocorticoid-receptor-corticosteroid complex that subsequently translocates across the nuclear membrane and binds to specific DNA sequences 6 . (ersjournals.com)
2013). A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway . (up.pt)
The cholinergic postsynaptic membrane is an acetyl-choline receptor-rich membrane mediating fast chemical communication at the nerve-muscle synapse. (iucr.org)
Structure of the ACh receptor in Torpedo postsynaptic membrane. (iucr.org)
c ) The 5.8 Å density map and superimposed 2.7 Å structure of the Torpedo receptor (PDB entry 6uwz ) obtained from detergent-solubilized protein complexed with α -bungarotoxin. (iucr.org)
While 16α-hydroxyestrone has relatively weak affinity for estrogen receptor, it has prolonged effect due to covalent binding to the receptor. (helsinki.fi)
Moreover, GEN treatment alleviated hepatic steatosis and lipid metabolism disorders through reducing the expression levels of mRNA related to fatty acid transport and synthesis and enhancing the mRNA expression levels of factors associated with fatty acid oxidation in FLHS layers, which may be achieved by activation of the G protein-coupled estrogen receptor-adenosine 5'-monophosphate (AMP)-activated protein kinase signaling pathways. (bvsalud.org)
For this purpose, a SLB functionalized with TCR and an adhesion ligand, was allowed to bind cell with pMHC and the corresponding adhesion receptor. (lu.se)
When the chylomicrons are reduced in TG content, they become remnants that are rapidly cleared by the liver (apoprotein E binds to the LDL receptor [LDL-R]). At this time, apoprotein C-II is passed to high-density lipoprotein (HDL) particles in the circulation. (medscape.com)

Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Unlike protein localization, identification of critical differences in membrane content has not yet been expanded to lipids, due to the difficulty of isolating domain-specific samples. (nih.gov)
PICK1 regulates the subcellular localization and surface expression of its PDZ-binding partners. (karger.com)
It is an important process of the cell that regulates the localization and function of numerous proteins. (epfl.ch)

Non-specific lipid-transfer proteins (nsLTPs) are involved in the movement of phospholipids, glycolipids, fatty acids, and steroids between membranes. (rcsb.org)
Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)
Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. (novusbio.com)
The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. (rndsystems.com)
However, these lipids can be transported throughout the bloodstream as lipoproteins when packaged with phospholipids and proteins (apoproteins). (medscape.com)

Here we provide the first evidence to suggest that tyrosine phosphorylation of Cav-1 and subsequent interaction among members of the Ras signaling pathway within the membrane lipid microdomains represent early signaling mechanisms of IL-1β in β-cells. (diabetesjournals.org)

Arf1 and Arf6 are the most divergent and most studied human Arf proteins that localize predominantly to the Golgi complex and plasma membrane respectively. (portlandpress.com)
Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex. (portlandpress.com)
It promotes lipid membrane association of the protein, for instance to the plasma membrane, Golgi apparatus, or inner nuclear membrane. (epfl.ch)

The microvilli are covered with glycocalyx , consisting of peripheral glycoproteins that can attach themselves to a plasma membrane via transmembrane proteins . (wikipedia.org)
This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)

Since the outer membrane is composed almost entirely of anionic lipopolysaccharide, with similar headgroup properties to POPG, such anionic lipid binding could prevent closure of OmpF channels, thereby increasing access of antibiotics that use porin-mediated pathways. (ox.ac.uk)

Both isoform 3 of the human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) and Traf2- and Nck-interacting kinase (TNIK) are putative effectors of Rap2 in mediating the activation of c-Jun N-terminal kinase (JNK) to regulate the actin cytoskeleton. (nih.gov)
Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)
Arf (ADP-ribosylation factor) GTP-binding proteins function in cells to regulate membrane traffic and structure. (portlandpress.com)
They serve as membrane-membrane, or membrane-cytoskeleton linkers, and regulate the formation of lipid domains in the plasma membrane. (rndsystems.com)
Like most biochemical processes in the cell, protein S-acylation is reversible to regulate the functions of acylated proteins. (epfl.ch)

Probing lipid mobility of raft-exhibiting model membranes by Fluorescence Correlation Spectroscopy. (rug.nl)

The advantages of homochiral membranes compared to those of heterochiral membranes were analysed in terms of specific recognition, optimal functions of enzymes, membrane fluidity and topological packing. (mdpi.com)
Because of this vital function, the microvillar membrane is packed with enzymes that aid in the breakdown of complex nutrients into simpler compounds that are more easily absorbed. (wikipedia.org)
Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. (novusbio.com)
Lysosomes contain dozens of different kinds of hydrolytic enzymes, which are active at the acidic pH maintained by proton pumps found in lysosomal membranes. (cellimagelibrary.org)
S-acylation is reversed by the enzymes acyl protein thioesterases (APTs). (epfl.ch)
There, the β tongue "dips" into the membrane and holds APT2 temporarily in place, which is necessary for it to be "met" by the enzymes that will acylate it. (epfl.ch)

High-resolution profiling of the ROS disk lipid composition gives new insights into how intricate membrane structure and protein activity are balanced within the ROS, and provides a model for future studies of other complex cellular structures. (nih.gov)
Furthermore, its protein-lipid composition, organization and size are subject to modification by physiological events, making it an important mediator of neuronal plasticity. (iucr.org)
Triglycerides are lipid compounds composed of a glycerol esterified to 3 fatty acid chains of varying length and composition. (medscape.com)
Changes in the lipid composition of the membranes of red blood cells and platelets may account for the other blood abnormalities that sometimes occur in sitosterolemia. (medlineplus.gov)

To understand these different sensitivities, we performed an extensive bioinformatics analysis of membrane protein structures and found that OmpF, and to a lesser extent FpvA and VDAC, have atypically high local densities of basic and acidic residues in their lipid headgroup-binding regions. (ox.ac.uk)
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding. (nih.gov)
Structures and Dynamics of Native-State Transmembrane Protein Targets and Bound Lipids. (nih.gov)
In order to gain a better understanding of the structural basis of the non-specific binding of hydrophobic ligands by nsLTPs and to investigate the plasticity of the fatty acid binding cavity in nsLTPs, seven high-resolution (between 1.3 A and 1.9 A) crystal structures have been determined. (rcsb.org)
These depict the nsLTP from maize seedlings in complex with an array of fatty acids.A detailed comparison of the structures of maize nsLTP in complex with various ligands reveals a new binding mode in an nsLTP-oleate complex which has not been seen before. (rcsb.org)
High- and low-resolution structures of the MORN1 protein from Trypanosoma brucei and homologous proteins from the parasites Toxoplasma gondii and Plasmodium falciparum were obtained using a combination of macromolecular crystallography, small-angle X-ray scattering, and electron microscopy. (biorxiv.org)
It is frustrating therefore that many crystal structures of membrane proteins so reveal non-proton electron densities around the protein's hydrophobic surfaces but usually only part of the lipids or detergents are seen, and so these electron densities cannot be unambiguous assigned. (ukri.org)
Use of these heavy atoms tagged lipids and detergents could in principle allow the 'phases' to be solved and to provide a new general route to 'phasing' crystal structures of membrane proteins. (ukri.org)
A structural genomics approach is used to determine to the structures of proteins belonging to other classes of ABC transporters. (rug.nl)

these lipids (predominantly TGs) are packaged by the intestinal mucosal cells into chylomicrons. (medscape.com)

Lipid binding attenuates channel closure of the outer membrane protein OmpF. (ox.ac.uk)
Here we use native mass spectrometry to explore lipid binding as a function of charge of the outer membrane porin F (OmpF). (ox.ac.uk)
Step two, the fusion peptides bind to each other, tilting and pinching the inner and outer surface of the membrane together, forming a hole. (nih.gov)
To infect a cell, spike proteins-projections on the virus's outer surface-bind to the cell's surface. (nih.gov)
The cell's outer membrane envelops the virus in a pocket-like structure called a vacuole, which encapsulates the virus and takes it inside the cell. (nih.gov)
A rocking motion of the peptides penetrates the outer layer of the membrane, thinning the spot to the point of breaking, as the action pinches the inner and outer lipid layers together. (nih.gov)
Mycobacteria outer membrane, rich in lipids and essential disaccharide precursors such as trehalose, has been the prime target for drug development against tuberculosis. (uml.edu)

SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. (novusbio.com)

To visualize new target proteins, a major drawback is usually the lack of sensitive and specific antibodies. (nature.com)
To do their work, APTs have to interact with the lipid membranes that their target proteins are bound to. (epfl.ch)

To truly enter or leave an organism, most substances must cross cell plasma membranes. (aqa.org.uk)
Purification and Proteomic Analysis of Plant Plasma Membranes. (lu.se)

We present a molecular dynamics simulation study on the binding mechanism of the membrane targeting domain of HIV-1 Gag protein. (elifesciences.org)
Measuring pico-Newton forces with lipid anchors as force sensors in molecular dynamics simulations. (mpg.de)

The structure and spontaneous curvature of clathrin lattices at the plasma membrane. (nih.gov)
Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature. (nih.gov)
Membrane morphologies induced by mixtures of arc-shaped particles with opposite curvature. (mpg.de)
A protein curvature for sensing touch. (mpg.de)
The headline says, "A massive supercomplex induces membrane curvature for cellular respiration. (evolutionnews.org)

PICK1 is a peripheral membrane protein conserved from Caenorhabditis elegans to the human. (karger.com)

As a result, gene transcription and protein synthesis are altered. (ersjournals.com)
Kocer, A., Walko, M., and Feringa, B.L. (2007) Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL. (rug.nl)
Resynthesized TGs can be used for plasma and cell organelle membrane synthesis. (medscape.com)
As an alternative, adipocytes can use TGs in membrane synthesis, which is similar to muscle. (medscape.com)

van den Bogaart, G., Krasnikov, V., and Poolman, B. (2006) Dual-color fluorescence burst analysis to probe protein diffusion through the mechanosensitive channel MscL. (rug.nl)

In human platelets, Rap2 was shown to interact with the cytoskeleton by binding the actin filaments. (nih.gov)
A number of additional effector proteins for Rap2 have been identified, including the RalGEFs RalGDS, RGL, and Rlf, which also interact with Rap1 and Ras. (nih.gov)
It is known that GSLs interact with both intracellular as well as exogenous proteins and are critical for membrane organisation, signalling, and recognition events. (springer.com)
It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. (novusbio.com)
The results of this study will be very useful in helping to understand the molecular details of how membrane proteins interact with lipids. (ukri.org)
There is a great deal of current interest in trying to understand the molecular details of how membrane proteins interact with lipids. (ukri.org)

The neutron reflectivity technique is applied to determine the adsorptive interaction of the 13.5-kDa actin-binding protein hisactophilin from Dictyostelium discoideum with lipid monolayers at a lateral pressure of 21 mN/m ≤ π ≤ 25 mN/m at the air-water interface. (hw.ac.uk)

Heide van der T., and Poolman, B. (2002) ABC transporters: one, two or four extracytoplasmic substrate-binding sites? (rug.nl)
The ABCG family of membrane-associated transporters: you don't have to be big to be mighty. (medlineplus.gov)

The results again demonstrate that the fatty acid of Gb 3 plays a pivotal role for the overall membrane organisation. (springer.com)
3 The tandem CBS domain 4 , which is linked to the nucleotide binding protein, plays a pivotal role in the regulation. (rug.nl)

Microvilli are covered in plasma membrane, which encloses cytoplasm and microfilaments . (wikipedia.org)

In the present study, we examined potential contributory roles of membrane-associated, cholesterol-enriched lipid rafts/caveolae and their constituent proteins (e.g., caveolin-1 [Cav-1]) as potential sites for IL-1β−induced nitric oxide (NO) release in the isolated β-cell. (diabetesjournals.org)
Disruption of lipid rafts (e.g., with cyclodextrin) markedly reduced IL-1β−induced gene expression of inducible NO synthase (iNOS) and NO release from β-cells. (diabetesjournals.org)
IL-1β treatment also increased (within 20 min) the translocation of H-Ras into lipid rafts. (diabetesjournals.org)

All biological membranes contain enantiomerically pure lipids in modern bacteria, eukarya and archaea. (mdpi.com)
Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)

the role of micelles in the absorption of lipids. (aqa.org.uk)
Structure and Stability of the Spinach Aquaporin SoPIP2;1 in Detergent Micelles and Lipid Membranes. (lu.se)

Our study suggests Myr insertion is involved in the sorting of membrane lipids around the protein binding site to prepare it for viral assembly. (elifesciences.org)
It is responsible for binding to new host cells and mediating fusion between the viral membrane, and the target cell membrane. (scienceblogs.com)
To keep using HIV-1 as an example, this precursor protein gets chopped in two, into a t rans m embrane gp41, which stays anchored in the viral membrane, and a s ub u nit gp120, which is noncovalently attached to gp41 like a lolly-pop. (scienceblogs.com)
Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration. (nih.gov)
Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)

The C18:1 ricinoleate with its hydroxyl group hydrogen bonding to Ala68 possibly mimics cutin monomer binding which is of biological importance. (rcsb.org)
Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
In my talk, I will show you some examples how such biological cues influence the dynamic properties of membranes. (lu.se)

Lysosomal function is critical for organismal homeostasis-mutations in genes encoding lysosomal proteins cause severe human disorders known as lysosomal storage diseases, and lysosome dysfunction is implicated in age-associated diseases including cancer, neurodegeneration and metabolic syndrome. (stanford.edu)
Photoreceptors rely on distinct membrane compartments to support their specialized function. (nih.gov)
Finally, the scientists brought their data together to work out how APT2 actually binds lipid membranes, which is essential to its capacity to perform its function in the cell. (epfl.ch)
This is a very important issue since many of these lipids affect the function of the membrane proteins tio which they are bound. (ukri.org)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)

Mass transport maintains the final diffusion gradients that bring substances to and from the cell membranes of individual cells. (aqa.org.uk)
Molecular mechanics underlying flat-to-round membrane budding in live secretory cells. (nih.gov)
Theyre coated in the membranes of the cells they budded off of. (scienceblogs.com)
The findings may inform the development of new technologies to combat infectious microbes and to insert medications, genes, and proteins into cells to treat diseases. (nih.gov)
Cells are enveloped in a membrane that protects from disease-causing organisms, while permitting nutrients to enter and waste to leave. (nih.gov)
Purifying the SNB-BAR proteins from yeast cells allows the acquisition of native homo and hetero SNX-BAR dimers while minimizing the toxicity and insolubility frequently encountered with non-native expression systems. (jove.com)
Immunologic and confocal microscopic evidence also suggested a transient but significant stimulation of tyrosine phosphorylation of Cav-1 in β-cells briefly (for 15 min) exposed to IL-1β that was markedly attenuated by three structurally distinct inhibitors of protein tyrosine phosphorylation. (diabetesjournals.org)
The researchers mixed exosomes from mesenchymal stem cells with fibrinogen, a protein involved in blood clotting. (scienceblog.com)
Excess plant sterols in red blood cells likely make their cell membranes stiff and prone to rupture, leading to a reduced number of red blood cells (anemia). (medlineplus.gov)

The identification of novel, high affinity AQP9 inhibitors in an intracellular binding site. (lu.se)

The prevalence of basement membranes in the tissues of Eumetazoans means that most cell types require attachment to survive. (wikipedia.org)
Researchers at the National Institutes of Health believe they have discovered how influenza viruses open a hole in the cell membrane to inject genetic material into the cell. (nih.gov)
As mentioned, microvilli are formed as cell extensions from the plasma membrane surface. (wikipedia.org)
Corticosteroids bind within the cell nucleus to regulators that control the transcription of various pro-inflammatory gene products and, therefore, are effective in controlling asthma by reducing the inflammation caused by these multiple mediators. (ersjournals.com)
In short, S-acylation on Cys-2 is essential for APT2 to be able to stably bind lipid membranes and deacylate its targets in the cell. (epfl.ch)
We have proposed that under conditions of severe osmotic stress (panel b, lower figure), the cell membrane contacts the nucleoid, which creates pools of proteins and hinders free diffusion through the cell. (rug.nl)
A large pool of research has been aimed at characterizing this interaction by measuring the binding kinetics and relating it to the T cell response. (lu.se)
In many T cell activation studies, the SLB contains the nickel-chelating lipid DGS-NTA(Ni) to functionalize the SLB with histidine-tagged proteins. (lu.se)
This is measured on individual cell-SLB contacts, providing an accurate new means of measuring binding affinity and to study differences in the 2D Kd in the cell population. (lu.se)
This indicates that TCR-pMHC binding in cell contacts depends significantly on the local environment and not only on the protein-protein interaction per se. (lu.se)
As cell membranes are both dynamic and compositionally complex, replicating these aspects in a model membrane are essential. (lu.se)
Sterolin is a transporter protein, which is a type of protein that moves substances across cell membranes. (medlineplus.gov)

We compare binding of natural hisactophilin exhibiting a myristic acid chain membrane anchor at the N-terminus (DIC-HIS) and a fatty acid-deficient genetic product expressed in Escherichia coli (EC-HIS). (hw.ac.uk)
Each sample's copurified lipids were subjected to untargeted lipidomic and fatty acid analysis. (nih.gov)

Particle-based membrane model for mesoscopic simulation of cellular dynamics. (mpg.de)

Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein. (nih.gov)

At the molecular level, such responses include changes in the transcription or translation of genes, and post-translational and conformational changes in proteins, as well as changes in their location. (wikipedia.org)
On the relationship between docking scores and protein conformational changes in HIV-1 protease. (mpg.de)

The researchers found that within the acidic environment of the vacuole, fusion peptides on the spike protein bind to the vacuole's inner membrane. (nih.gov)

In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484). (novusbio.com)
The aim of this study was to evaluate the beneficial effects and potential mechanisms of genistein (GEN) on production performance impairments and lipid metabolism disorders in laying hens fed a high-energy and low-protein (HELP) diet. (bvsalud.org)
Fatty liver hemorrhagic syndrome (FLHS) is a nutritional and metabolic disease that seriously threatens the health and performance of laying hens, which is characterized by hepatic steatosis and lipid metabolism disorders. (bvsalud.org)
As an isoflavone phytoestrogen, genistein (GEN) exerts many beneficial functions, including alleviating lipid metabolism disorders and anti-inflammatory properties. (bvsalud.org)

A litmus test for classifying recognition mechanisms of transiently binding proteins. (mpg.de)

Most Ras proteins contain a lipid modification site at the C-terminus, with a typical sequence motif CaaX, where a = an aliphatic amino acid and X = any amino acid. (nih.gov)
Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation. (nih.gov)
Arfs accomplish this task through modification of membrane lipids and the recruitment of proteins, including coat proteins and actin, to membrane surfaces. (portlandpress.com)

by similarity, to similar proteins with structure having catalytic activity. (nih.gov)

A hemi-fission intermediate links two mechanistically distinct stages of membrane fission. (nih.gov)
Membrane fission by dynamin: what we know and what we need to know. (nih.gov)

Insertion events only occur with one of the membrane models, showing a combination of surface charge and internal membrane structure modulate this process. (elifesciences.org)
Of note, with these extraction procedures, GSLs are categorized by their glycan structure, whereas the lipid tails are not determined (Fahy et al. (springer.com)
With its PDZ domain interacting with membrane proteins and its BAR domain binding to lipids, the unique structure of PICK1 enables it to couple membrane proteins to protein-trafficking machinery. (karger.com)
This study introduces pretreatment methods via extruders that disrupt the molecular structure of post-consumer polyester waste, improving binding sites for enzyme reaction. (uml.edu)

Most commonly, protein phosphorylation is catalyzed by protein kinases, ultimately resulting in a cellular response. (wikipedia.org)
We have found that Arf6 acts through activation of type I phosphatidylinositol 4-phosphate 5-kinases to generate phosphatidylinositol 4,5-bisphosphate and that this activity is instrumental in facilitating the actin cytoskeletal rearrangements and alterations in endosomal membrane trafficking observed with increased Arf6 activation. (portlandpress.com)
Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)

Probing the link between pancratistatin and mitochondrial apoptosis through changes in the membrane dynamics on the nanoscale. (mpg.de)

Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. (rndsystems.com)

It is demonstrated that only the natural hisactophilin DIC-HIS is capable of mediating the strong binding of monomeric actin to the monolayer, where it forms a layer of about 40 Å thickness corresponding to the average diameter of actin monomers. (hw.ac.uk)
2015). The peroxisomal protein import machinery displays a preference for monomeric substrates . (up.pt)
2011). PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14 . (up.pt)

Anatomy48

Organisms13

Diseases3

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes61

Disciplines and Occupations3

Technology, Industry, Agriculture7

Information Science3

Health Care1

Vesicles12

Bilayer5

Molecules20

Nucleic3

Interactions19

Bilayers4

Cytoplasmic2

Native lipids2

Receptors6

Receptor17

Localization4

Phospholipids5

Signaling pathway1

Golgi3

Transmembrane proteins2

Anionic1

Regulate5

Model membranes1

Enzymes6

Composition4

Structures9

Predominantly1

Outer7

Subcellular1

Target proteins2

Plasma membranes2

Molecular dynamics2

Curvature5

Peripheral1

Synthesis4

Fluorescence1

Interact6

Actin-binding1

Transporters2

Plays a pivotal role2

Cytoplasm1

Rafts3

Bacteria2

Micelles2

Viral5

Biological3

Function5

Cells9

Intracellular1

Cell12

Fatty acid2

Particle1

Regulates1

Conformational changes2

Acidic1

Metabolism4

Mechanisms1

Modification3

Similar proteins1

Fission2

Structure4

Kinases3

Mitochondrial1

Amino acid1

Monomeric3