• In addition to maintaining specific organelle structures, phase separation enables hub proteins to assemble signalosomes which promote the speed of signaling outputs 11 , 12 . (nature.com)
  • Since these intrinsically disordered regions, which are conformationally dynamic and do not adopt stable secondary or tertiary structures, are often essential for mediating the phase transition of proteins 9 , it is therefore of interest to consider if the C-terminus of IRS-1 is involved in phase separation and to further delineate such implications upon insulin/IGF signaling. (nature.com)
  • Recently, it has been recognized that membrane-bound ribosomes are crucial for biogenesis of integral membrane proteins in E. coli , thus renewing interest in ribosome targeting to and association with the membrane in this organism. (rupress.org)
  • Absorbed Vitamin B 12 is transported via specific B 12 binding proteins, transcobalamin I and II to the various tissues. (nih.gov)
  • It is then transported by the transcobalamin binding proteins. (nih.gov)
  • The centromere is aided in binding sister chromatids together by several proteins called cohesins and condensins. (coursehero.com)
  • The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
  • Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
  • Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
  • The binding of autoantibodies to Dsg proteins induces a separation of neighbouring keratinocytes, in a process known as acantholysis. (nih.gov)
  • Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). (cellsignal.com)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
  • Lipids and proteins are attacked and oxidized, resulting in loss of chlorophyll and carotenoids and in leaky membranes which allow cells and cell organelles to dry and disintegrate rapidly. (weedscience.org)
  • Membraneless organelles, composed of RNA and RNA binding proteins, are an important interface for RNA biology research in neurodegeneration. (weizmann.ac.il)
  • We previously showed that microRNA malfunction is mechanistically related to ALS and discovered that RNA-binding proteins, which are mutated in ALS, disrupt the microRNA biogenesis machinery. (weizmann.ac.il)
  • In eukaryotic cells, various proteins and nucleic acids are orderly arranged in the nucleus and cytoplasm ( Handwerger and Gall, 2006 ). (aspetjournals.org)
  • Recently, it was revealed that the ordered localization of proteins and nucleic acids in the membraneless intracellular microenvironment is modulated by liquid-liquid phase separation (LLPS) ( Shin and Brangwynne, 2017 ). (aspetjournals.org)
  • In a recent study, Wang and colleagues reported that a significant fraction of cancer-associated fusion proteins display a common structural topology, including an N-terminal phase separation-prone region (PS) from one parent protein and a C-terminal DNA-binding domain (DBD) from the other. (bvsalud.org)
  • While the specific function of polyglutamine-binding protein 1 is not well understood, it is believed to play a role in processing and transporting RNA, a chemical cousin of DNA that serves as the genetic blueprint for the production of proteins. (medlineplus.gov)
  • Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication. (cdc.gov)
  • Here, we found that the C-terminus of IRS-1 undergoes liquid-liquid phase separation (LLPS). (nature.com)
  • This finding provides a novel concept that paraspeckles formed by liquid-liquid phase separation potentially affect drug metabolism via negative regulation of PXR function. (aspetjournals.org)
  • From lysis buffers to separate lymphocytes and red blood cells to reagents that can help enrich your desired immune cell subpopulations by positive selection or depletion, our offerings span every part of your sample preparation workflow. (bdbiosciences.com)
  • The human sets can enrich for B lymphocytes, CD4 and CD8 T lymphocytes, and NK cells, while the mouse sets can enrich for these and dendritic cells or hematopoietic progenitor cells. (bdbiosciences.com)
  • Depletion of human T and B lymphocytes and NK cells. (bdbiosciences.com)
  • Separation of antigen-specific lymphocytes. (wikidata.org)
  • The resultant inflammatory mediators that are produced induce migration of lymphocytes, eosinophils, neutrophils, and mast cells to the BMZ. (medscape.com)
  • A thickened, fibrotic capsule is observed, with complete destruction of the cortex (although a few small clusters of adrenocortical cells surrounded by lymphocytes may remain) and relative sparing of the medulla. (medscape.com)
  • Phenylcarbamates, pyridazinones, triazines, triazinones, and uracils are herbicides that inhibit photosynthesis by binding to the Q B -binding niche on the D1 protein of the photosystem II complex in chloroplast thylakoid membranes. (weedscience.org)
  • Inhibition of fatty acid synthesis presumably blocks the production of phospholipids used in building new membranes required for cell growth. (weedscience.com)
  • BD IMag™ Particles are nanometer-sized super-paramagnetic particles with monoclonal antibody or streptavidin covalently bound to their surface. (bdbiosciences.com)
  • The BD Pharm Lyse™ Solution lyses red blood cells following monoclonal antibody staining. (bdbiosciences.com)
  • Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
  • Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (cellsignal.com)
  • We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
  • Extracting DNA from whole blood using this method works by capturing DNA on magnetic beads coated with a matrix of silica for binding nucleic acids. (autogen.com)
  • At the D/A interfaces, the electrons will be transferred to A, provided that the energy gain overcomes the exciton binding energy. (hindawi.com)
  • We investigate the role of excitons and the exciton binding energy E B , relevant for the effectiveness of charge separation in solar cells, as well as the temperature-dependent bandgap energy E g which is used as an indicator for crystal phase transitions. (monash.edu)
  • Binding selectivity describes how a ligand may bind more preferentially to one receptor than another. (wikipedia.org)
  • A receptor is a protein molecule, embedded in either the plasma membrane or the cytoplasm of a cell, to which one or more specific kinds of signalling molecules may bind. (wikipedia.org)
  • The specificity of a receptor is determined by its spatial geometry and the way it binds to the ligand through non-covalent interactions, such as hydrogen bonding or Van der Waals forces. (wikipedia.org)
  • Parafollicular (PF) cells secrete 5-HT in response to stimulation of a G-protein-coupled Ca 2+ receptor (CaR) by increased extracellular Ca 2+ (↑[Ca 2+ ] e ). (jneurosci.org)
  • Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • This cell isolation kit targets non-B cells for removal based on specific surface antigens. (stemcell.com)
  • These enrichment reagents contain a cocktail of monoclonal antibodies used to label antigens expressed by all unwanted cells. (bdbiosciences.com)
  • This signal results in the generation of B-cell clones that produce antibodies against antigens located at the basement membrane zone (BMZ), initiating a type II Gell and Coombs hypersensitivity reaction. (medscape.com)
  • Using a murine model of tolerance induced by repeated exposure to a low dose of aerosolized antigen, we show an important contribution by CD4(+) T cells in the establishment and maintenance of tolerance. (nih.gov)
  • Strikingly, separation of the TGF-beta(+) cells from the rest of the cells allowed the TGF-beta(-) cells to proliferate in response to antigen. (nih.gov)
  • We propose a model of antigen-induced tolerance that involves cell-cell contact with regulatory CD4(+) T cells that coexpress membrane-bound TGF-beta and FOXP3. (nih.gov)
  • The antibodies of immunoglobulin G (IgG), immunoglobulin A (IgA), and/or immunoglobulin M (IgM) bind to the antigen and initiate complement activation. (medscape.com)
  • We describe the isolation of Jurkat cell-specific membrane rafts using 2% Triton X-100. (upenn.edu)
  • Cell culture isolation of SARS-CoV-2 is possible, but the Centers for Disease Control and Prevention (CDC) recommends that clinical laboratories not attempt this unless it is performed in a biosafety level 3 (BSL-3)-certified laboratory. (medscape.com)
  • The depletion cocktail in this kit does not contain antibodies against CD43, which can be expressed on malignant cells in chronic lymphocytic leukemia (CLL) samples. (stemcell.com)
  • Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. (stemcell.com)
  • Pemphigus is diagnosed on the basis of either IgG or complement component 3 deposits (or both) at the keratinocyte cell membrane, detected by direct immunofluorescence microscopy of a perilesional biopsy, with serum anti-Dsg1 or anti-Dsg3 antibodies (or both) detected by ELISA. (nih.gov)
  • Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)
  • Specific antibodies, if present, bind to the 21-hydroxylase during this time. (medscape.com)
  • Penicillamine, which contains nitrogen and sulphur donor atoms, is used as this type of ligand binds more strongly to copper ions than to calcium and magnesium ions. (wikipedia.org)
  • In response to a paper claiming to create a metal halide perovskite p-n homojunction solar cell, we have shown how redistribution of mobile ions in the material would screen out the electric field normally present at the junction. (imperial.ac.uk)
  • In contrast to the chemical synapses used by neurons , electrical synapses , in the case of cardiac muscle, are created by ions flowing from cell to cell, known as an action potential . (wikidoc.org)
  • Immunoprecipitation assays using PXR-overexpressing HepG2 (ShP51) cells revealed that PXR interacts with not only DAZAP1 but also NEAT1_2, a long noncoding RNA included in the paraspeckle, and that the interaction between PXR and NEAT1_2 was disassociated by rifampicin. (aspetjournals.org)
  • This is reminiscent of the structural topology of transcription factors and led to the hypothesis that the PS-DBD fusions form aberrant transcriptional condensates through phase separation, which was supported through transcriptomic data analysis and cellular condensate assays. (bvsalud.org)
  • IGF-1 stimulation enhanced IRS-1 phase separation which then recruited downstream effectors to form insulin/IGF signalosomes. (nature.com)
  • Stimulation of PF cells with ↑[Ca 2+ ] e increased the activity of immunoprecipitated PKCζ (but not PKCι/λ), and the activity of this PKCζ was inhibited by pseudoZ. (jneurosci.org)
  • We suggest that PKC isoforms mediate secretion of 5-HT by PF cells in response to stimulation of the CaR. (jneurosci.org)
  • The Big Easy" EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. (stemcell.com)
  • Visit our EasySep™ Cell Separation Magnets page to compare the different options and select the appropriate magnet for your research. (stemcell.com)
  • The BD IMag™ Reagent is used to magnetically label these cells, which are then migrated to the BD IMag™ Cell Separation Magnet, leaving behind a pure and untouched subpopulation of cells. (bdbiosciences.com)
  • After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet, and the depleted and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. (bdbiosciences.com)
  • Importantly, metabolic disease-derived G972R mutation results in a reduced ability of LLPS, potentially implicating the involvement of aberrant IRS-1 phase separation in various metabolic disorders. (nature.com)
  • We are interested in understanding the potential connection between stress granules, one type of membraneless organelles and aberrant phase separation in disease, including discovery of mechanisms for nucleation of insoluble aggregates that are present in patient brain and spinal cords. (weizmann.ac.il)
  • LLPS, which was originally studied in the field of physical chemistry, has been extended to the field of cell biology since the 2010s. (aspetjournals.org)
  • In eukaryotic cells , DNA is found in structures called chromosomes. (coursehero.com)
  • No. 557983), and mononuclear cells were identified by scatter profile. (bdbiosciences.com)
  • The buffer does not contain a fixative agent, so leukocytes remain visible after red blood cell lysis. (bdbiosciences.com)
  • This lysis step releases the nuclei and mitochondria from the white blood cells, which are collected via centrifugation, leaving the RNA in the supernatant liquid (Figure 1b). (autogen.com)
  • Methods that are often used for scaling such in vitro data to in vivo predictions are the well stirred model and the parallel tube model, and these models contain plasma free fraction terms that account for the effect of plasma binding on the clearance of compounds ( Pang and Rowland, 1977 ). (aspetjournals.org)
  • Contrary to expectation, the SSUL modules do not replace RbcS, but bind close to the equatorial region of Rubisco between RbcL dimers, linking Rubisco molecules and inducing phase separation into a liquid-like matrix. (nature.com)
  • It aims to provide an understanding of the basic chemical properties of molecules that make life possible and a description of the key components of the cell and their biochemical interactions. (manchester.ac.uk)
  • To achieve these goals, our laboratory has focused on the development of scalable technologies to detect, measure, and manipulate molecules and circuits, both at the sub-cellular level, and at the level of cell populations. (stanford.edu)
  • Other cellular structures are duplicated during G2, such that each replicated daughter cell produced during mitosis will have all necessary organelles (such as mitochondria, endoplasmic reticulum, etc. (coursehero.com)
  • To date, many strategies have been investigated to accomplish this goal, and some of these strategies rely on the differences between the cellular compositions of the targeted cells and those of the nontargeted cells. (biosyn.com)
  • Furthermore, the differences in cellular trafficking profiles and the pH of endosomes between normal and cancer cells have also been exploited for selective drug delivery to a specific compartment in the intracellular space of cancer vs. normal cells. (biosyn.com)
  • The specific cellular or molecular binding site relevant to the action of IAA and the auxin-mimicking herbicides has not been identified. (weedscience.com)
  • Beyond the single cell, we also strive to map cellular ensembles, such as brain tissue. (stanford.edu)
  • We develop tools that can be used to construct cell and tissue atlases, and map the cellular circuits that give rise to function and behavior. (stanford.edu)
  • Charge-transfer excitons (CTXs) at organic donor/acceptor interfaces are considered important intermediates for charge separation in photovoltaic devices. (uni-marburg.de)
  • The obtained E B values of 20-24 meV for Cs-containing mixed perovskite compounds are below the ones of 24-32 meV and 36-41 meV for pure methylammonium lead iodide (MAPbI 3 ) and bromide (MAPbBr 3 ), respectively, and, thus, facilitate charge-carrier separation in photovoltaic applications. (monash.edu)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • The separation of the epithelium from the underlying tissues within the BMZ may be the result of direct cytotoxic action or the effect of lysosomal proteolytic enzymes. (medscape.com)
  • Tissues and Cells. (lu.se)
  • Here we show that in cells depleted of the SRP protein, Ffh or the translocon component SecE, the ribosomal targeting pathway is blocked downstream and unprecedented, membrane-bound FtsY-ribosomal complexes are captured. (rupress.org)
  • Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)
  • Immunoblots from membrane and cytosolic fractions of isolated PF cells revealed conventional (α, βI, and γ), novel (δ and ε), and atypical (ι/λ and ζ) PKCs. (jneurosci.org)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • The ability of the B-subunit of cholera toxin to bind GM1 has been exploited to visualize membrane rafts by confocal microscopy in patching and capping experiments. (upenn.edu)
  • Biochemically, membrane rafts are isolated by solubolization in ice-cold Triton X-100 and separation of the low-buoyant density fractions from soluble material on sucrose density gradients. (upenn.edu)
  • An alternative mechanism of action has been proposed involving destruction of the electrochemical potential of the cell membrane, but the contribution of this hypothesis remains in question. (weedscience.com)
  • Binding selectivity is defined with respect to the binding of ligands to a substrate forming a complex. (wikipedia.org)
  • These differences could be in the expressed surface receptors (i.e., the absence or presence of certain receptors), the metabolism profiles (i.e., different enzyme expression or intracellular trafficking), the site/location of the cells (i.e., circulating in blood stream vs. organ), and the nature of the cells (i.e., normal vs. cancerous). (biosyn.com)
  • For example, tumor cells have certain upregulated receptors, enzymes, and other metabolic features that are not present in normal cells in the body. (biosyn.com)
  • Chronic T-cell exhaustion is marked by the progressive upregulation of coinhibitory receptors, PD-1 being one of the most prominent, but also other coregulated receptors such as LAG-3 ( 4, 5 ). (aacrjournals.org)
  • Visit our magnetic separation application page to learn more about the principles behind positive and negative selections and to view sample data. (bdbiosciences.com)
  • Tumor persistence leads to sustained exposure of T cells to neoantigens-this results in repeated cycles of activation which culminate in a state of severe T-cell dysfunction or exhaustion, characterized by distinct epigenetic and transcriptional profiles ( 1-3 ). (aacrjournals.org)
  • Tumor cell-intrinsic programming and signals from geographically and temporally changing microenvironments both contribute to this variability. (bvsalud.org)
  • Enzymes, which are crucial to controlling how cells replicate in the human body, could be the very ingredient that encourages DNA to spontaneously mutate -- causing potentially permanent genetic errors, according to new research. (sciencedaily.com)
  • However, the roles of phase separation in the regulation of many other signaling pathways await further exploration. (nature.com)
  • Mechanism of 53BP1 activity regulation by RNA-binding TIRR and a designer protein. (nih.gov)
  • We use live cell imaging and APEX proximity labeling, proteomics and transcriptomics to characterize membraneless organelles. (weizmann.ac.il)
  • Interphase is characterized by cell growth and DNA replication , while M is the phase of the cell cycle in which the chromosomes of the cell prepare for the cell to divide into two new cells. (coursehero.com)
  • Desired cells are simply collected into a new tube and can immediately be used in downstream applications such as nucleic acid extraction, flow cytometry or culture. (stemcell.com)
  • Downstream processing with emphasis on chromatographic separation of biomolecules. (lu.se)
  • When a cell begins to copy itself, it must undergo DNA replication, in which the first step is the separation of the two DNA strands so that each can be used as a template for a new DNA. (sciencedaily.com)
  • The strand separation is enabled by a type of enzyme called a 'helicase', which binds to one of the DNA strands and pulls it through itself, thereby forcing apart the DNA. (sciencedaily.com)
  • Moreover, IRS-1 was found to form high-molecular-mass complexes in different types of cells 30 . (nature.com)
  • Molecular basis for the inhibition of the methyl-lysine binding function of 53BP1 by TIRR. (nih.gov)
  • The goal of our laboratory is to develop, scale up, and broadly disseminate molecular technologies for mapping cells and functional circuits. (stanford.edu)
  • Serum samples containing more than 100 mg/dL of hemoglobin can cause non-specific binding in serologic tests. (cdc.gov)
  • Centrifuge samples for serum separation within 4 hours of sample collection. (cdc.gov)
  • I am interested in charge carrier behaviour in semiconducting materials and devices, particularly in solar cells and systems for energy conversion. (imperial.ac.uk)
  • DRIFTFUSION is a 1D time dependent drift diffusion code suitable for simulating solar cells, including those containing mobile ionic defects. (imperial.ac.uk)
  • If the transport of ionic defects is responsible for hysteresis in perovskite solar cells, why is hysteresis removed or reduced by changing the contact materials? (imperial.ac.uk)
  • We developed a set of optoelectronic measurements that enable us to infer how the ionic charge distribution in hybrid perovsktie solar cells changes as they are operated. (imperial.ac.uk)
  • Subpicosecond transient absorption spectroscopy is a powerful tool used to clarify the exciton and carrier dynamics within the organic solar cells (OSCs). (hindawi.com)
  • This is in a sharp contrast with inorganic solar cells (ISCs), in which the photoexcitation directly creates free carriers in the active layer. (hindawi.com)
  • We verify these results using temperature-dependent electroreflectance spectroscopy, which is a very reliable technique for the direct and non-destructive determination of optical resonances of the absorber layer in complete solar cells. (monash.edu)
  • Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. (sigmaaldrich.com)
  • After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. (sigmaaldrich.com)
  • I suggest this chronology as a continuum, rather than a temporally bound trajectory. (lu.se)
  • Despite originating from a common driver mutation, Ewing sarcoma cells display significant variations in transcriptional activity, both within and among tumors. (bvsalud.org)
  • X-ray crystal structures of TIRR and a designer protein bound to 53BP1 now reveal a mechanism in which an intricate binding area centered on an essential TIRR arginine residue blocks the methylated-chromatin-binding surface of 53BP1. (nih.gov)
  • Normally, A always binds to T, and G always binds to C due to the different structures of these biomolecules and the different number of hydrogen bonds formed between these base pairs. (sciencedaily.com)
  • such as those in the brain, polyglutamine-binding protein 1 is found in structures called RNA granules. (medlineplus.gov)
  • The Frenkel exciton in organic materials has high binding energy (=0.2-0.5 eV) due to the electrostatic potential between them. (hindawi.com)
  • The average diet supplies about 5 to 15 mcg/day of Vitamin B 12 in a protein-bound form that is available for absorption after normal digestion. (nih.gov)
  • Despite their clonal origins, tumors eventually develop into complex communities made up of phenotypically different cell subpopulations, according to mounting evidence. (bvsalud.org)
  • Interphase comprises the gap 1 (G1), synthesis (S), and gap 2 (G2) phases, in which the cell grows and replicates its genetic material. (coursehero.com)
  • Through these mechanisms, polyglutamine-binding protein 1 is thought to help control the way genetic information is used (gene expression) in neurons. (medlineplus.gov)
  • During the G2 phase, a structure in the cytoplasm of animal cells that coordinates the formation of microtubules, called a centrosome , allows cell division to proceed during reproduction. (coursehero.com)
  • The newest 3M solution, 3M™ Harvest RC, utilises fibrous anion exchange (AEX) chromatography to efficiently separate cells, cell debris and DNA from the harvest fluid containing the target product. (3m.com.au)
  • 3M™ Polisher ST is a fully encapsulated single-use anion exchange (AEX) product intended to reduce host cell protein impurities, viruses and other negatively charged contaminants in flow-through polishing chromatography. (3m.com.au)
  • The ↑[Ca 2+ ] i activates a signaling cascade that involves, sequentially, calcium-calmodulin, nitric oxide synthase, guanylyl cyclase, and protein kinase G. This signal transduction pathway enables Cl − to pass through vesicular channels and act as a counterion for the transport of H + , so that the vesicles of secretogogue-stimulated cells become acidic. (jneurosci.org)
  • It is shown that the extent of microsomal binding is correlated with lipophilicity, but that basic compounds show a different behavior to acidic and neutral compounds. (aspetjournals.org)
  • Microsomal binding is shown to be best predicted using a model where logP is used for basic compounds, and logD 7.4 is used for acidic and neutral compounds. (aspetjournals.org)
  • and the M phase, the fourth phase of the cell cycle, when a cell divides. (coursehero.com)
  • A fifth phase has been identified, G0, in which the cell is resting just after mitosis but still carrying out normal functions. (coursehero.com)
  • A cell can remain in the G0 phase indefinitely, awaiting a signal to return to the normal cell cycle. (coursehero.com)
  • The amount of time spent in any given phase of the cell cycle is dependent upon the type of cell and the environmental conditions in which the cell lives. (coursehero.com)
  • The M phase consists of mitosis, in which the cell divides, producing two new, identical cells. (coursehero.com)
  • The length of this particular phase is the most variable among different cell types. (coursehero.com)
  • When the cell receives signals for division, it moves to the S phase. (coursehero.com)
  • Once the DNA has been replicated, the cell moves to the second gap phase. (coursehero.com)
  • Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. (sigmaaldrich.com)
  • Here, the white cell nuclei and mitochondria are treated with a denaturation buffer containing chaotropic salt and a protease. (autogen.com)
  • Attenuated humoral responses in HIV after SARS-CoV-2 vaccination linked to B cell defects and altered immune profiles. (cdc.gov)
  • their evolution from distinct cell lineages. (lu.se)
  • We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)