Bind antibodies blocking. Medical search. Frequent questions

This family is named after the reticulocyte binding proteins in P. vivax, a parasite that only infects reticulocytes (immature red blood cells) expressing the Duffy antigen. (wikipedia.org)
The ability of the Illinois bird sera to block the binding of the MAbs to WNV antigen was compared to the blocking ability of normal chicken serum (Vector Laboratories, Burlingame, CA). The percentage inhibition value was calculated as previously described ( 7 ). (cdc.gov)
Tumor-shed antigen CA125 blocks complement-mediated killing via suppression of C1q-antibody binding. (ncbcs.org)
Lateral-flow antigen tests have been introduced, but they are used primarily as binary qualitative tests and report only binding between antibody and antigen rather than neutralization 7 . (nature.com)
DEKnull is a synthetic DBP based antigen that has been engineered through mutation to enhance induction of blocking inhibitory antibodies. (rcsb.org)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. (stemcell.com)
The H129.19 monoclonal antibody specifically binds to the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e. (bdbiosciences.com)
We designed an IgA complex model with IgA binding to skin basement membrane zone (BMZ) antigen. (scirp.org)
This standardizes the amount of captured antibody allowing their sorting by ranking their reactivity with the labeled antigen. (cdc.gov)
This agent inhibits chemotaxis of eosinophils and locomotion of neutrophils and impairs complement-dependent antigen-antibody reactions. (medscape.com)
Many microorganisms have mechanisms that impair antibody production by inducing suppressor cells, blocking antigen processing, and inhibiting lymphocyte mitogenesis. (msdmanuals.com)

Enzyme-linked immunosorbent assays with infectious virus particles at low pH or in the presence of metals demonstrated that all tested antibodies do not bind to this contracted form, akin to what was observed with the MNV-bile complex. (uni-luebeck.de)
Recent studies have shown that assays performed with these MAbs detect antibodies to WNV in taxonomically diverse North American avian species ( 7 ). (cdc.gov)
When diluted, this reagent can be used for blocking non-specific binding when rabbit secondary antibodies are used in immunohistochemistry (IHC) assays. (fishersci.com)
Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays. (nature.com)
Assays that measure antibody titer and neutralizing capability exist but are not compatible with home use. (nature.com)
Traditional affinity-based immunoassays, such as enzyme-linked immunosorbent assays (ELISAs) 5 , can quantitatively measure antibody titer, but due to inherent complexity and instrumentation, they require a centralized laboratory for diagnostics. (nature.com)
Antibody neutralizing capabilities are traditionally measured in cell-based live viral infection assays that require BSL3 facilities 6 . (nature.com)
We aimed to develop a sensor technology that can quantitatively measure nAb responses against different isolates of SARS-CoV-2, be adapted for an all-in-solution multiwell format and provide rapid results in 1 hour, which is faster than established ELISA assays measuring SARS-CoV-2 antibody titer (~6 hours) or cell-based neutralization assays (~one to several days). (nature.com)
Functional binding assays with monoclonal antibodies were used to interrogate the available epitopes in DEKnull. (rcsb.org)
We leverage a number of proprietary immunization strategies for tough target antibody discovery along with a suite of premium downstream characterization assays to ultimately deliver a panel of fully characterized lead molecules ready for development. (news-medical.net)
Prevalence of interference is usually less in assays holding blocking agents, which counteract or inhibit the interference. (medgadget.com)
The application segment is led by antibody capture assays with a share of 39.8% in 2020, owing to high, precise, and accurate results with least interference obtained via immunoassay interference blockers. (medgadget.com)
Title : Effects of pre-analytical heat treatment in factor VIII (FVIII) inhibitor assays on FVIII antibody levels Personal Author(s) : Boylan, B.;Miller, C. H. (cdc.gov)

Here we report two monoclonal antibodies (mAbs) cloned from memory B cells of patients recently recovered from COVID-19, and both mAbs specifically bind to the spike (S) protein of SARS-CoV-2, block the binding of receptor binding domain (RBD) of SARS-CoV-2 to human angiotensin converting enzyme 2 (hACE2), and effectively neutralize S protein-pseudotyped virus infection. (medrxiv.org)
These plus-strand RNA viruses have T = 3 icosahedral protein capsids with 90 pronounced protruding (P) domain dimers, to which antibodies and cellular receptors bind. (uni-luebeck.de)
Reticulocyte binding protein homologs (RHs) are a superfamily of proteins found in Plasmodium responsible for cell invasion. (wikipedia.org)
A P. falciparum protein complex called PfRH5-PfCyRPA-PfRipr (RCR) is known to bind basigin via the tip of RH5. (wikipedia.org)
We demonstrate that targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to CD40 (αCD40.RBD) induces significant levels of specific T and B cells, with long-term memory phenotypes, in a humanized mouse model. (nature.com)
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. (nature.com)
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein binds to the angiotensin-converting enzyme 2 (ACE-2) receptor on target cells and is the immunodominant target of neutralizing antibodies (nAbs) identified from convalescent and postvaccination plasma 3 . (nature.com)
We adapted a designed coronavirus spike RBD biosensor 9 consisting of a switchable lucCageRBD protein containing a 'cage' domain, which in the closed state of the sensor binds a 'latch' domain containing the picomolar affinity RBD binding LCB1 protein 10 , and a lucKey protein that binds to the open state of the sensor, reconstituting luciferase activity 11 . (nature.com)
An important aspect of antibodies is that they bind tightly and specifically to another protein. (edu.au)
Antibody-based therapies, or 'biologics', harness this property of antibodies, enabling them to bind to a protein involved in disease. (edu.au)
We can then extract the gene sequences encoding the nanobodies and use this to produce millions of types of nanobodies in the laboratory, and then select the ones that best bind to the spike protein. (edu.au)
ANSTO's Australian Synchrotron and the Monash Ramaciotti Centre for Cryo-Electron Microscopy were critical resources in the project, allowing the research team to map how the nanobodies bound to the spike protein and how this impacted the virus' ability to bind to its human receptor. (edu.au)
Researchers designed "miniproteins" that bound tightly to the SARS-CoV-2 spike protein and prevented the virus from infecting human cells in the lab. (nih.gov)
Researchers are testing monoclonal antibody therapies that bind to the spike protein and neutralize the virus. (nih.gov)
Researchers led by Dr. David Baker of the University of Washington set out to design synthetic "miniproteins" that bind tightly to the coronavirus spike protein. (nih.gov)
Using cryo-electron microscopy, the team was able to build detailed pictures of how two of the miniproteins bound to the spike protein. (nih.gov)
The Plasmodium vivax vaccine candidate Duffy Binding Protein (DBP) is a protein necessary for P. vivax invasion of reticulocytes. (rcsb.org)
The DEKnull protein retains binding to conformationally dependent inhibitory antibodies. (rcsb.org)
DEKnull has reduced immunogenicity to polymorphic regions responsible for strain-specific immunity while retaining conserved protein folds necessary for induction of strain-transcending blocking inhibitory antibodies. (rcsb.org)
Binds to protein and other compounds containing SH group. (medscape.com)
The platinum complex also can bind to nucleus and cytoplasmic protein. (medscape.com)
Three-train L-LTP was also blocked by inhibitors of guanylyl cyclase or cGMP-dependent protein kinase (PKG). (jneurosci.org)
Conversely, either NO or cGMP analogs paired with one-train tetanization produced late-phase potentiation, and the cGMP-induced potentiation was blocked by inhibitors of protein or RNA synthesis and an inhibitor of PKG, but not by an inhibitor of PKA. (jneurosci.org)
To test a possible downstream target of PKG, we examined changes in phospho-CRE-binding protein (phospho-CREB) immunofluorescence in the CA1 cell body area and obtained results similar to those of the electrophysiology experiments. (jneurosci.org)
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)
With antibody-drug conjugates like trastuzumab deruxtecan (Enhertu), a drug is chemically linked to an antibody that targets a specific protein on the cancer cell surface. (cancer.gov)
Cyclosporine binds to cyclophilin, an intracellular protein, which, in turn, prevents formation of interleukin 2 and the subsequent recruitment of activated T cells. (medscape.com)
Cyclophosphamide is an alkylating agent of the nitrogen mustard family that exerts cytotoxic effects by binding to nucleic acids and crosslinking DNA and RNA strands and inhibiting protein synthesis. (medscape.com)

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. (thermofisher.com)
Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). (thermofisher.com)

The spike binds to ACE2 receptors on the cell surface. (nih.gov)
mainly target the viral spike protein's relatively mutable S1 region, with which the virus binds to host-cell receptors. (indiatimes.com)
These results indicate that none of the integrins known to be present on mouse eggs or to be ADAM receptors are essential for sperm-egg binding/fusion, and thus, egg integrins may not play the role in gamete fusion previously attributed to them. (lu.se)
Most toxins bind to specific target cell receptors. (msdmanuals.com)

The heterologous antibodies administered bind with venom antigens and block their deleterious effects. (medscape.com)
The position of bands on the nitrocellulose strips allows this antibody reactivity to be associated with specific viral antigens. (cdc.gov)
During incubation, if HIV-1 antibodies are present in the specimen, they will bind to the viral antigens bound to the nitrocellulose strips. (cdc.gov)
If antibodies to any of the major HIV-1 antigens are present in the specimen in sufficient concentration, bands corresponding to the position of one or more of the following HIV-1 proteins (p) or glycoproteins (gp) will be seen on the nitrocellulose strip: p17, p24, p31, gp41, p51, p66, gp120, gp160 (number refers to apparent molecular mass in kilodaltons). (cdc.gov)
Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)

Typically, they harness an optical phenomenon to detect the binding of a solution molecule (analyte) to its immobilized partner (ligand) on a sensor and record the output as a "sensorgram", which tracks the binding signal in arbitrary units proportional to mass sensed at the surface of the biosensor as a function of time. (intechopen.com)
It has also been reported in blocking of ligand binding. (thermofisher.com)
Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. (thermofisher.com)

These cutting-edge biophysical tools are revolutionizing our understanding of molecular-level processes because they reveal an entire binding profile between unlabeled reactants and dissect it into kinetic components from which an affinity can be deduced. (intechopen.com)
The antibody was purified by affinity chromatography. (biolegend.com)
Our traditional hybridoma discovery approach triages antibody candidates in a high-throughput manner by specificity, affinity, and oftentimes functionality early in the workflow. (news-medical.net)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
In this study we have profiled a panel of mouse antihuman CD40 monoclonal antibodies (MoAbs), regarding their CD40 binding affinity and epitope-specificity relative to the CD40L binding in relation to their cellular activating potential. (lu.se)

A humanized immunoglobulin G1 (IgG1) monoclonal antibody, risankizumab blocks interleukin (IL) 23 by binding to its p19 subunit. (medscape.com)
Plasmapheresis (plasma exchange) and intravenous immunoglobulin, which are procedures to remove abnormal antibodies from your blood. (medlineplus.gov)
The separated HIV-1 proteins are elecrotransferred from gel to a nitrocellulose membrane, which is then washed, blocked (to minimize nonspecific immunoglobulin binding), and packaged. (cdc.gov)
This attenuation of specific immunoglobulin E antibody is associated with increased specific immunoglobulin G4 antibodies, which are likely to play a protective role, leading to a form of natural tolerance. (cdc.gov)

Published in PNAS , the research is part of a consortium-led effort, bringing together the expertise of Australian academic leaders in infectious diseases and antibody therapeutics at WEHI, the Doherty Institute and the Kirby Institute . (edu.au)
The study, published in the journal Immunity, could lead to the development of a broad coronavirus vaccine and related antibody therapeutics. (indiatimes.com)
First-generation immuno-oncological antibody therapeutics involved checkpoint modulators targeting common T-cell checkpoints such as PD-1 and CTLA-4. (news-medical.net)
Therapeutics of this nature capitalize on the exquisite specificity of antibody biologics to directly target tumor cells with cytotoxic agents (for example, ADCs) or indirectly target tumor cells by actively recruiting other immune cells (such as T-cells and macrophages) via multi-specific antibody formats. (news-medical.net)

Serum samples were tested for antibodies to WNV by epitope-blocking enzyme-linked immunosorbent assay (ELISA), according to the protocols of Blitvich et al. (cdc.gov)
The sensitivity and specificity of the antibodies were evaluated by enzyme-linked immunosorbent assay and immunohistochemistry. (hindawi.com)

In total, 94 birds, representing 5 orders and 19 species, were positive for antibodies to WNV ( Table 1 ). (cdc.gov)
Flavivirus antibodies were detected in 13 species, and one Australasian gannet ( Morus serrator ) from one site was positive for antibodies to Ross River virus. (who.int)

Together with the family of erythrocyte binding-like proteins (EBLs) they make up the two families of invasion proteins universal to Plasmodium. (wikipedia.org)
From experimentation on partial proteins, RHs are known to contain enterocyte-binding and nucleotide-sensing domains (EBD and NBD) that may partially overlap. (wikipedia.org)
Nanobodies - which are tiny immune proteins - could provide an alternative to conventional antibody treatments for COVID-19. (edu.au)
Antibodies are key infection-fighting proteins in our immune system. (edu.au)
Nanobodies are unique antibodies - tiny immune proteins - produced naturally by alpacas in response to infection. (edu.au)
This technology allowed them to custom build proteins and predict how they would bind to the receptor. (nih.gov)
When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. (rndsystems.com)
Visualization of the human immunoglobulins specifically bound to HIV-1 proteins is accomplished in situ by using a series of reactions with goat anti-human IgG conjugated with biotin, avidin conjugated with horseradish peroxidase (HRP), and the HRP substrate 4-chloro-1-naphthol. (cdc.gov)

Antibody-mediated inhibition of Rabbit Polyclonal to ELOVL5 Notch2 signalling didn't have an effect on proliferation, but elevated migration of SGHPL-5 cells and principal CTBs. (e-core.org)
The production of the polyclonal antibody against IL-33 provides a good tool for studying the biofunctions of IL-33. (hindawi.com)

A much better crystal structure for an N-terminal receptor-binding domain (presumably the same as EBD) was published in 2014. (wikipedia.org)
The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. (nature.com)
Using a large library of miniproteins, they identified designs that could potentially bind within a key part of the coronavirus spike called the receptor binding domain (RBD). (nih.gov)

In a functional ELISA, 1-6 ng/mL of this antibody will block 50% of the binding of 300 ng/mL of Biotinylated Recombinant Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1δ Fc Chimera (Catalog # 1134‑RA ) coated at 1 µg/mL (100 µL/well). (rndsystems.com)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)

Research conducted in our laboratory and in others has demonstrated that dapsone inhibits the binding of neutrophils to IgA. (scirp.org)

The Cambridge Biotech HIV-1 Western Blot Kit, when used as directed, will detect antibodies to HIV-1 when present in human serum or plasma. (cdc.gov)

This can induce the antibody-mediated adverse drug effect of heparin-induced thrombocytopenia (HIT). (fu-berlin.de)
In principle, a vaccination strategy that can induce such antibodies is likely to provide broad protection against a diverse spectrum of betacoronaviruses,' said Burton. (indiatimes.com)
We believe that this analysis of several mouse anti-CD40 antibodies can be used to develop strategies for producing new human anti-CD40 antibodies that can more effectively induce or block B-cell proliferation. (lu.se)

The presence of serum thyroid antibodies usually indicates an autoimmune thyroid disorder, but elevated levels may also be detected in other conditions. (medscape.com)

Associate Professor Wai-Hong Tham , who led the research, said the establishment of a nanobody platform at WEHI allowed an agile response for the development of antibody-based therapies against COVID-19 . (edu.au)
These viruses mutate at a modestly high rate, creating a significant challenge for the development of vaccines and antibody therapies against them. (indiatimes.com)

binding of the RM4-5 antibody can be blocked by the clone GK1.5 antibody. (stemcell.com)
The H129.19 antibody blocks binding of the anti-mouse CD4 antibodies GK1.5 and RM4-5, but not RM4-4 antibody. (bdbiosciences.com)

The DEKnull mutations do not cause peptide backbone shifts within the polymorphic loop, or at either the DBP dimerization interface or DARC receptor binding pockets, two important structurally conserved protective epitope motifs. (rcsb.org)
The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. (thermofisher.com)
Due to our unique combination of engineered mouse models, proprietary immunization techniques, and premium high-throughput and high-content screening capabilities, we specialize in high homology targets, constrained epitope targeting, and tough cell surface targets, all of which exist within the immuno-oncological antibody research space. (news-medical.net)
Sera from each period ( n = 185 and n = 693) were screened in an epitope blocking enzyme immunoassay for flavivirus antibody detection. (who.int)

CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162).The HAE-1f antibody has been shown to recognize human CD62E and to be useful for flow cytometry and in vitro blocking. (biolegend.com)

Blocking of Recombinant Mouse Rae-1 beta is observed at a similar antibody concentration, however, this antibody does not block the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha or Rae-1 epsilon in this assay. (rndsystems.com)
Substances that modify quantifiable concentration of analytes or change antibody binding can conceivably result in immunoassay interference. (medgadget.com)
An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. (bdbiosciences.com)

Recently developed cell-free tools can measure antibody titers but cannot necessarily evaluate neutralization, and none of the currently available tools have estimated neutralization activity against the emerging set of SARS-CoV-2 VOCs 8 . (nature.com)
Monitoring antibody titers is important to evaluate the disease progression/regression over time, as well as among different patients, but the same assay should be used for this purpose. (medscape.com)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. (abcam.com)

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852) and EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782). (stemcell.com)

Antibody 19.3 was compared in one-site and competitive ELISAs for oligomer binding specificity. (jneurosci.org)

Abveris is a contract research organization specializing in critical reagent and therapeutic antibody discovery for a wide range of targets, including those in the immune-oncology research space. (news-medical.net)

One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)

RPA-T4 blocks HIV binding and mixed lymphocyte reaction. (thermofisher.com)

7 ). ELISAs were performed with two monoclonal antibodies (MAbs), 3.1112G and 2B2. (cdc.gov)

drugs that bind their targets tightly can be administered at lower levels than those that bind weakly and thus are less likely to exhibit undesirable side-effects and are more cost-effective. (intechopen.com)

But these antibodies, which are derived from immune system molecules, are large and not ideal for delivery through the nose. (nih.gov)
But in someone with myasthenia gravis, the body's own immune system makes antibodies that block the binding of acetylcholine to the muscle. (medlineplus.gov)
Monoclonal antibodies, which can also help decrease your body's immune system responses. (medlineplus.gov)

Detection of interference may involve usage of a different assay, or quantification before and after treatment with supplementary blocking reagents. (medgadget.com)
All specimens were tested using the Synthetic Peptide Enzyme Immunoassay (EIA) (Genetic Systems HIV-1/HIV-2 Peptide EIA) for the detection of antibody to human immunodeficiency virus type 1 or type 2 (HIV-1 or HIV-2) or both (Bio-Rad Laboratories, Redmond, WA). (cdc.gov)

The sera are tested with the appropriate antibody to check for possible cross-reactivities and supplied undiluted with 0.08% sodium azide as a preservative. (fishersci.com)

A cell culture model, based on wild-type and specific integrin knockout cell lines lacking the integrin subunits αv, β1 or β3, was used to investigate the susceptibility to WNV, and to evaluate binding and replication efficiencies of four distinct strains (New York 1999, Uganda 1937, Sarafend and Dakar). (nih.gov)
Binding, however, was not affected by the expression of the integrins in question, and integrin blocking antibodies failed to have any effect. (nih.gov)
Using alpha3 integrin null eggs, we found that the alpha3beta1 integrin was not essential for sperm-egg binding and fusion. (lu.se)
Furthermore, neither anti-mouse beta3 integrin function-blocking monoclonal antibody (mAb) nor alphav integrin function-blocking mAb inhibited sperm binding to or fusion with beta1 integrin null eggs. (lu.se)

Additionally, we demonstrate that a single dose of the αCD40.RBD vaccine, injected without adjuvant, is sufficient to boost a rapid increase in neutralizing antibodies in convalescent non-human primates (NHPs) exposed six months previously to SARS-CoV-2. (nature.com)

With the availability of coronavirus disease 2019 (COVID-19) vaccines, the rise of more transmissible and pathogenic virus mutants 1 and known time-dependent declines in immunity following infection 2 , there is a need to determine the degree of serological antibody protection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (nature.com)
Serological antibody tests (ideally home-based diagnostics) are critical to evaluate the response to vaccination and viral infection 2 . (nature.com)
Researchers have identified nanobodies that effectively blocked the SARS-CoV-2 virus from entering cells in pre-clinical models of COVID-19 infection. (edu.au)
Blocking entry of SARS-CoV-2 into human cells can prevent infection. (nih.gov)
They appear to block SARS-CoV-2 infection at least as well as monoclonal antibodies but are much easier to produce and far more stable, potentially eliminating the need for refrigeration. (nih.gov)
Scientists have found antibodies in the blood of certain Covid-19 donors that can block infection from a broad set of coronaviruses, specifically in people who have recovered from the SARS-CoV-2 virus and were then vaccinated. (indiatimes.com)
In a study published last year, the team found that some human antibodies can bind to this site on SARS-CoV-2 in a way that apparently disrupts viral fusion and blocks infection. (indiatimes.com)
We conclude that integrins are involved in WNV infection but not at the level of binding to target cells. (nih.gov)

Even more precise targeting is on the horizon: for example, technologies that limit antibody activity to the tumor microenvironment and reduce cytotoxic activity in healthy tissue, resulting in a holistic attack on tumor cells with minimal adverse effects. (news-medical.net)
We enter this year with tremendous progress advancing KVA12123 into clinical development, receiving IND clearance from the FDA, and initiating a first-in-human clinical trial of our VISTA blocking immunotherapy that addresses immune resistance in the tumor microenvironment. (tmcnet.com)

Knowledge of individual immunity to SARS-CoV-2 is useful not only to determine personal actions but also to guide early therapy of patients and evaluate the efficacy of antibody treatment and vaccines over time against emerging viral variants of concern (VOCs) 3 . (nature.com)
By combining the two leading nanobodies into this nanobody cocktail, we were able to test its effectiveness at blocking SARS-CoV-2 from entering cells and reducing viral loads in preclinical models," she said. (edu.au)
Griffithsin's antiviral activity stems from its ability to bind terminal mannoses present in high-mannose oligosaccharides and crosslink these glycans on the surface of the viral envelope glycoproteins. (mdpi.com)

Here, we demonstrate that MNV undergoes reversible conformational changes at pH 5.0 that are nearly identical to those observed when bile binds. (uni-luebeck.de)

This model explains 14% of the variation in antibody prevalence. (cdc.gov)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)

The following product was used in this experiment: CD4 Monoclonal Antibody (RPA-T4), PE-Cyanine7, eBioscience™ from Thermo Fisher Scientific, catalog # 25-0049-42, RRID AB_1659695. (thermofisher.com)
The following product was used in this experiment: CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-1152-82, RRID AB_469046. (thermofisher.com)

An inhibitor of NO synthase blocked L-LTP induced by three-train tetanization and reduced L-LTP induced by four-train tetanization, whereas an inhibitor of PKA was more effective in blocking four-train L-LTP than three-train L-LTP. (jneurosci.org)
Despite a rather similar domain-recognition profile, the MoAbs blocked the CD40L binding to a varying degree, with MoAb 5C3 being the poorest inhibitor. (lu.se)

This antibody was developed for use in flow cytometry. (bdbiosciences.com)

The CD40-CD40L interaction plays a critical role in both humoral and cellular immune responses and interfering antibodies have been suggested as an effective approach for the treatment of lymphomas and autoimmune diseases. (lu.se)

Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. (thermofisher.com)

Received U.S. Food and Drug Administration (FDA) acceptance of Kineta's Investigational New Drug application (IND) to evaluate its VISTA blocking immunotherapy KVA12123 as a potential treatment for patients with advanced solid tumors. (tmcnet.com)
Under this collaboration, Kineta will evaluate the safety, tolerability, pharmacokinetics and anti-tumor activity of KVA12123, its novel anti-VISTA monoclonal antibody, alone and in combination with KEYTRUDA® (pembrolizumab), Merck's anti-PD-1 therapy, in patients with advanced solid tumors. (tmcnet.com)
Antithyroid antibody studies are used to evaluate for autoimmune thyroid problems. (medscape.com)

Structural studies of several of the antibodies when bound to S2 illuminated their common binding sites and modes of binding, providing key information that should aid the development of future vaccines targeting this region. (indiatimes.com)
Targeted rational vaccine strategies could take advantage of this molecular information of the interactions of these antibodies with the S2 domain to inform the design of pan-betacoronavirus vaccines," Wilson said. (indiatimes.com)

Australian researchers have identified neutralising nanobodies that block the SARS-CoV-2 virus from entering cells in preclinical models. (edu.au)
Single domain heavychain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. (cdc.gov)

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. (nih.gov)

PfRH4 is known to bind complement receptor 1. (wikipedia.org)
C1q-engagement with IgG and IgM sort antibodies is the initiating step of classical complement-mediated immunity. (ncbcs.org)

SN-38 binds to and stabilizes the topoisomerase I-DNA complex and prevents the relegation of DNA after it has been cleaved by topoisomerase I, inhibiting DNA replication. (medscape.com)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)

The ingestible or inhalable antibody prevents allergic reactions and asthma in adults, by binding to molecules which would otherwise cause allergy. (medindia.net)

the entire P domain rotates by ~90° and contracts onto the shell, the P domain dimers rotate about each other, and the structural equilibrium of the epitopes at the top of the P domain shifts toward the closed conformation, which favors receptor binding while blocking antibody binding. (uni-luebeck.de)

Development of antibodies to FIX treatment products (inhibitors) is rare and related to the type of causative variant present. (cdc.gov)

Anatomy25

Organisms18

Diseases4

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Phenomena and Processes50

Disciplines and Occupations2

Information Science3

Health Care5

Antigen12

Assays13

Protein30

Monoclonal antibody reacts2

Receptors4

Antigens5

Ligand3

Affinity5

Immunoglobulin4

Therapeutics4

Enzyme-linke2

Positive for antibodies2

Proteins8

Polyclonal2

Receptor bindin3

ELISA2

Inhibits1

Detect antibodies1

Induce3

Serum1

Therapies2

GK1.52

Epitope4

Vitro1

Concentration3

Conjugate1

Titers2

Isotype control1

Clone1

Specificity1

Reagent1

IgG11

Lymphocyte1

ELISAs1

Tightly1

Immune system3

Detection2

Supplied undiluted1

Integrin4

Convalescent1

Infection8

Tumor microenvironment2

Viral3

Conformational changes1

Prevalence1

Abcam1

EBioscience2

Inhibitor2

Flow cytometry1

CD401

Applications Tested2

Evaluate3

Vaccines2

Nanobodies2

Complexes1

Complement2

Replication1

Cytometric analysis3

Prevents1

Epitopes1

Inhibitors1