DeficiencyGeneEnzymesGenesDegradationRecombinantAmino acidsNucleic acidsMembraneReagentsEncodesImmunoTherapySpecificityPolysaccharidesBindFusionConjugationTranscriptionalPeroxisomalAntibodiesImmuneBacillusVitroBacteriaBiosynthesisPurificationDeletionPhageNucleusEffectorPrecursorStabilityWidelyBiologicalSuitableDrugsComplexesReactionStructuralEffectivelyTargetSpecificCellResistanceStructureStableIntensity
Deficiency4
- D-Bifunctional protein deficiency is an autosomal recessive peroxisomal fatty acid oxidation disorder. (wikipedia.org)
- Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). (nih.gov)
- Rapid whole-genome sequencing identifies a homozygous novel variant, His540Arg, in HSD17B4 resulting in D-bifunctional protein deficiency disorder diagnosis. (nih.gov)
- D-bifunctional protein deficiency caused by HSD17B4 gene mutation in a neonate. (bvsalud.org)
Gene7
- The gene encoding ArsR/methyltransferase fusion protein, arsR M , was amplified and expressed in Escherichia coli BL21 (DE3), and this strain showed resistance to arsenic in the present of 0.25-6 mM As(III), aresenate, or pentavalent roxarsone. (biomedcentral.com)
- The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. (nih.gov)
- However, in the vast majority of prokaryotes, including M. tuberculosis , CoaB and CoaC are encoded by a single gene to produce a fused bifunctional enzyme (CoaBC). (nature.com)
- In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
- Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. (frontiersin.org)
- RESULTS: We expressed the gene encoding Enterococcus faecium phage IME199 DNA polymerase (IME199 DNAP) in Escherichia coli BL21(DE3), and characterized protein function. (bvsalud.org)
- The responsible genetic defect occurs in 1 of at least 12 genes involved in peroxisomal formation or protein import (the PEX gene family). (msdmanuals.com)
Enzymes1
- Was previously thought to be a copper protein, but it is now known that the enzymes from soybean (Glycine max), the mold Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. (cathdb.info)
Genes2
- Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
- 10% transformation frequency), which reside in genes undergoing strong diversifying selection that encode large membrane proteins, likely as an immune invasion tactic. (drexel.edu)
Degradation3
- PROteolysis TArgeting Chimeras (PROTACs) are small molecule degraders that are bifunctional, allowing them to bind both a protein of interest and the cell's machinery for degradation. (imperial.ac.uk)
- The review concludes with discussion of the next steps for the area of targeted protein degradation and other emerging areas of scientific interest triggered by the invention of PROTACs. (imperial.ac.uk)
- They promote association of E3 with specific target proteins tagged for degradation via the PROTEASOME. (bvsalud.org)
Recombinant2
- Bifunctional recombinant protein SDF1-GP. (leuphana.de)
- Escherichia coli is a host bacterium widely used for the production of recombinant proteins, is the cell factory of choice for in vitro studies of phage protein function. (bvsalud.org)
Amino acids2
Nucleic acids1
- 1994). The initial reaction results in the formation of a reactive intermediate, which gives off hydrochloric acid to form phosgene, which is then free to react with cellular macromolecules (including GSH, proteins, and nucleic acids) or conjugate with water to form carbon dioxide and hydrochloric acid (Ade et al. (cdc.gov)
Membrane8
- Compact disc73 is a bifunctional glycosylphosphatidylinositol (GPI)-anchored membrane proteins which features as ecto-5-nucleotidase and a membrane receptor for extracellular matrix proteins (ECM). (biopaqc.com)
- Small angle neutron scattering for the study of solubilised membrane proteins. (ibs.fr)
- Change of dynamics of raft-model membrane induced by amyloid-beta protein binding. (ibs.fr)
- Membrane Fusion Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- This graph shows the total number of publications written about "Membrane Fusion Proteins" by people in Harvard Catalyst Profiles by year, and whether "Membrane Fusion Proteins" was a major or minor topic of these publication. (harvard.edu)
- Below are the most recent publications written about "Membrane Fusion Proteins" by people in Profiles. (harvard.edu)
- The cation channel mucolipin-1 is a bifunctional protein that facilitates membrane remodeling via its serine lipase domain. (harvard.edu)
- Crystal structure of the membrane fusion protein CusB from Escherichia coli. (harvard.edu)
Reagents3
- The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
- Hetero-bifunctional Reagents is a group of reagents that have an NHS ester (OSu) group that reacts with a primary amino group and a maleimide group that reacts with a thiol (SH group). (dojindo.com)
- Homo-bifunctional Reagents are a group of reagents with two NHS esters. (dojindo.com)
Encodes1
- Ortholog in C. albicans is ENP2 , encodes an protein. (cdc.gov)
ImmunoTherapy1
- M7824 is an investigational bifunctional fusion protein immunotherapy that is currently in clinical development, including potential registration studies, for multiple difficult-to-treat cancers. (pharmatimes.com)
Specificity3
- Small engineered scaffold proteins (ESPs) gain more and more popularity as biological drugs, due to their specificity and applicability in diagnostics and therapy. (kth.se)
- Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
- This difference in autotransporter proteins could possibly contribute to the bacteria's host specificity and the different diseases they cause [9]. (kenyon.edu)
Polysaccharides2
- Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
- The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
Bind4
- Enzymatic activity of D-BP fails if the protein cannot effectively bind the cofactor NAD+, as shown in the G16S mutation. (wikipedia.org)
- From this library, it was possible to achieve protein modules with the ability to simultaneously bind to its intended target as well as to human serum albumin (HSA), a feature that has been shown to increase the binder's half-life in the body. (kth.se)
- It can be used to bind two proteins in close proximity to each other, or to make a compound having an amino group reactive with an amino group. (dojindo.com)
- Platinum complex also can bind to nucleus and cytoplasmic protein. (medscape.com)
Fusion10
- SunRock Biopharma S.L. a biopharmaceutical company that is developing a portfolio of innovative bispecific antibodies to treat incurable cancer, and Ellipses Pharma Limited a global drug development company focused on accelerating the development of new oncology treatments, announce that they have entered into an exclusive licensing agreement for SRB22, a fully human bifunctional HER3:TRAIL fusion protein, which going forward will be known as EP0017. (pharmaceutical.report)
- EP0017 is a first-in-class bifunctional fusion protein with a dual mechanism of action. (pharmaceutical.report)
- Since its inception in 2014, SunRock has developed a comprehensive pipeline of seven therapeutic antibodies, bispecific antibodies and fusion proteins with proven therapeutic effects against several types of tumours that currently lack a treatment. (pharmaceutical.report)
- The innovative spirit of SunRock defines the development of all its molecules, that are the result of a disruptive technological platform that has led to pioneer therapies with an innovative format, including first-in-class bispecific antibodies and fusion proteins that target both novel and well-known therapeutic targets. (pharmaceutical.report)
- Bintrafusp alfa is a first-in-class bifunctional fusion protein composed of the extracellular domain of the TGF-βRII receptor (a TGF-β 'trap') fused to a human immunoglobulin G1 monoclonal antibody blocking PD-L1. (bmj.com)
- After proving these new fusion proteins have similar binding kinetics to the target, as well as comparable enzymatic activities, as their free counterpart, the cytotoxic effects were put to the test in vitro . (kth.se)
- The evident advantage of these fusion proteins and their competency to be functionally produced in E. coli as well as the possibility to avoid an additional step of conjugation or coupling of affinity proteins to cytotoxic payloads, makes this approach a promising alternative for current procedures and another reason why ESPs are on the rise. (kth.se)
- It is a bifunctional fusion protein. (globaldata.com)
- A Novel Bifunctional Fusion Protein, Vunakizumab-IL22, for Protection Against Pulmonary Immune Injury Caused by Influenza. (aidstar-one.com)
- Our bifunctional fusion protein M7824 has the potential to bring new answers to patients living with cancer. (pharmatimes.com)
Conjugation1
- However, apparent cross-reactivity in the double gel diffusion experiments was due to new antigenic determinants formed by isocyanate s after conjugation with proteins. (cdc.gov)
Transcriptional1
- Functional prediction analyses suggested that ArsR M is a difunctional protein with transcriptional regulation and methyltransferase activities. (biomedcentral.com)
Peroxisomal1
- The D-BP protein contains a peroxisomal targeting signal 1 (PTS1) unit at the C-terminus allowing for its transport into peroxisomes by the PTS1 receptor. (wikipedia.org)
Antibodies2
- Hapten-specific IgE antibodies were detected in the sera of English short-hair strain guinea pigs immunized with either toluene diisocyanate -human serum albumin (TDI-HSA) or hexamethylene diisocyanate -HSA (HDI-HSA) when these sera were analyzed by the 168 hr passive cutaneous anaphylaxis (PCA) technique followed by intravenous challenges with conjugates of respective ligands coupled to an unrelated carrier protein, transferrin. (cdc.gov)
- urinary proteins and specific IgE antibodies (3). (cdc.gov)
Immune1
- It was therefore apparent that immune responses of guinea pigs immunized with protein conjugates of bifunctional isocyanate s were heterogeneous and involved multiple specificities for hapten, carrier protein, and new antigenic determinants. (cdc.gov)
Bacillus1
- L-cystine-binding protein TcyAGene Names: tcyAResearch Areas: OthersOrganism: Bacillus subtilis (strain 168)AA. (joplink.net)
Vitro1
- In this study, the radiolabeling efficiency, in vitro stability, and biodistribution of immunoconjugates with eight different bifunctional chelators labeled with 64 Cu were compared. (acs.org)
Bacteria3
- These newly developed protein binders, showing high affinity towards their targets as well as keeping their initial binding to HSA, present another possibility to combine the advantages of small engineered scaffold proteins with those of typical larger proteins, allowing for more convenient production in bacteria leading to lower production costs and making them ideal candidates for future therapeutics. (kth.se)
- The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. (frontiersin.org)
- The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
Biosynthesis1
- The biosynthesis of CoA is performed in five steps, with the second and third steps being catalysed in the vast majority of prokaryotes, including M. tuberculosis , by a single bifunctional protein, CoaBC. (nature.com)
Purification1
- Purification of proteins from natural phages is often time-consuming, cumbersome and low yielding. (bvsalud.org)
Deletion1
- A UMP synthase bifunctional protein was constructed with a deletion of the suspected linker region joining the two catalytic domains. (unboundmedicine.com)
Phage1
- Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA. (ibs.fr)
Nucleus1
- 1 nucleus with a static residual quadrupole coupling, also for the water-1H magnetization, even in systems with high enhanced I-spin relaxation is observed at I-spin Larmor frequencies water content, as long as the protein component is immobilized matching an eigenfrequency of the static S-spin Hamiltonian. (lu.se)
Effector1
- While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). (frontiersin.org)
Precursor1
- Precursor of AMP + diphosphate + 4-coumaroyl- protein. (cdc.gov)
Stability2
- The monofunctional OPRT protein was less stable than the ODC protein at 45 degrees C. Growth of transfected cells in 6-azauridine resulted in striking increases in activity and temperature stability for the monofunctional ODC protein. (unboundmedicine.com)
- The increased stability of the bifunctional protein may be a factor in its evolutionary selection in mammalian cells. (unboundmedicine.com)
Widely1
- A divalent reagent is a reagent having two functional groups and is widely used in immunoassays and protein research. (dojindo.com)
Biological2
- Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological. (aidstar-one.com)
- For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)
Suitable1
- While all the macrocyclic bifunctional chelators are suitable for molecular imaging using 64 Cu-labeled antibody conjugates, NOTA and sar-CO 2 H show significant advantages over the others in that they can be radiolabeled rapidly at room temperature, under dilute conditions, resulting in high specific activity. (acs.org)
Drugs1
- In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
Complexes1
- Exploration of the supramolecular interactions involving tris-dipicolinate lanthanide complexes in protein crystals by a combined biostructural, computational and NMR study. (ibs.fr)
Reaction1
- Segments of the human UMP synthase cDNA coding for the orotate phosphoribosyl transferase (OPRT) and orotidylate decarboxylase (ODC) domains of the bifunctional protein UMP synthase were produced by polymerase chain reaction techniques and cloned into a eukaryotic expression vector. (unboundmedicine.com)
Structural2
- SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
- Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex. (ibs.fr)
Effectively1
- This dual modality means that they can be used to effectively target specific proteins for removal from the cell. (imperial.ac.uk)
Target2
- Bifunctional molecules that are designed to recruit E3 UBIQUITIN LIGASE to a specific target protein. (bvsalud.org)
- Proteolysis targeting chimera consist of a target protein ligand connected via a linker to an E3 ligand. (bvsalud.org)
Specific1
- A 28.6-kDa ODC protein band and a 24.4-kDa OPRT band were detected on western blots with UMP synthase-specific polyclonal antiserum. (unboundmedicine.com)
Cell1
- A bifunctional alkylating agent, once activated to aquated form in cell it binds to DNA, resulting in interstrand and intrastrand cross-linking. (medscape.com)
Resistance1
- Bifunctional protein ArsRM contributes to arsenite methylation and resistance in Brevundimonas sp. (biomedcentral.com)
Structure3
- CATH: Protein Structure Classification Database by I. Sillitoe, N. Dawson, T. Lewis, D. Lee, J. Lees, C. Orengo is licensed under a Creative Commons Attribution 4.0 International License . (cathdb.info)
- Characteristics of individual transport pathways, including their geometry, physico-chemical properties and dynamics are instrumental for understanding of structure-function relationships of these proteins, for the design of new inhibitors and construction of improved biocatalysts. (plos.org)
- By channel we mean a pathway leading throughout the protein structure, without any interruption by an internal cavity, with both sides open to the surrounding solvent. (plos.org)
Stable1
- Both OPRT and ODC activities in the monofunctional proteins were less heat stable than in the bifunctional UMP synthase protein. (unboundmedicine.com)
Intensity1
- The bifunctional MPQDs prepared under the most optimal parameters have a typical diameter of 35 nm and a saturation magnetization of 4.35 emu/g at room temperature and exhibit strong photoluminescence intensity. (springer.com)