If the catalyst is a moderate base such as hydroxide ion or an alkoxide, the aldol reaction occurs via nucleophilic attack by the resonance-stabilized enolate on the carbonyl group of another molecule. (wikipedia.org)
Transition-metal-free synthesis of 4-pyrones via TfOH-promoted nucleophilic addition/cyclization of diynones and water has been developed. (preprints.org)
Protein sequences3
Advances in DNA sequencing and machine learning are providing insights into protein sequences and structures on an enormous scale 1 . (nature.com)
Protein sequences vary by more than ten orders of magnitude in thermodynamic folding stability 2 (the ratio of unfolded to folded molecules at equilibrium). (nature.com)
First, the pocket, conservation, structural and energy profiles, and physicochemical properties of (non)covalently bound cysteines were collected from both protein sequences and 3D structures of protein-ligand complexes. (uni-freiburg.de)
Polypeptide2
mRNA display is an entire in vitro selection method that takes advantage of cell-free translation systems for the generation of polypeptide sequences. (medscape.com)
or conjugate, complex, or fusion protein or fusion polypeptide including the same disclosed amino acid sequence derived from bacterium (e.g., mycoplasma, anaplasma, etc. (patentsencyclopedia.com)
Amino2
Most AMPs are cationic polypeptides, usually smaller than 100 amino acids in length, with enormous sequence diversity. (biomedcentral.com)
The cDNA display proteolysis method is fast, accurate and uniquely scalable, and promises to reveal the quantitative rules for how amino acid sequences encode folding stability. (nature.com)
Structural1
Compared to traditional ML models with only sequenced-based features or only 3D structural features, HyperCys is more accurate at predicting hyper-reactive druggable cysteines. (uni-freiburg.de)
Experiments1
Assaying one library (up to 900,000 sequences in our experiments) requires one week and reagents costing about US$2,000, excluding the cost of DNA synthesis and sequencing. (nature.com)
Approach1
The latter approach involves a sequence of steps which is roughly approximated by Figure 1 : An unknown structure is isolated from some source. (nih.gov)
Diversity1
For totally or partially randomized peptide or protein libraries, the much higher diversity provides a big advantage for exploring the sequence space to a greater depth for the identification of target-binding partners. (medscape.com)
Cell-free1
This method combines the strengths of cell-free molecular biology and next-generation sequencing and requires no on-site equipment larger than a quantitative PCR (qPCR) instrument. (nature.com)