Beta subunit of casein kinase ii. Medical search. Frequent questions

Chester N, Yu IJ, Marshak DR (1995) "Identification and characterization of protein kinase CKII isoforms in HeLa cells. (proteinkinase.biz)
v) As observed with EF-1δ, the casein kinase II β subunit (CKIIβ) was specifically phosphorylated by UL13 in vitro, while the level of phosphorylation of CKIIβ by UL13 was greatly diminished when a serine residue at position 209, which has been reported to be phosphorylated by cdc2, was replaced with alanine. (elsevierpure.com)

Modulation of the activity of the enzyme cAMP-dependent protein kinase. (systemsbiology.net)

CONTAINS InterPro DOMAIN/s: Casein kinase II, regulatory. (riken.jp)
CK2 (casein kinase 2) is an ubiquitously expressed and highly conserved serine/threonine protein kinase that forms a tetramer containing two catalytic (α and/or α´) subunits and two regulatory β subunits [ 1 ]. (biomedcentral.com)
Protein kinase CK2 (Casein kinase II) is an ubiquitous and highly conserved protein serine/threonine kinase that is typically found in tetrameric complexes consisting of two catalytic (alpha and/or alpha\') subunits and two regulatory beta subunits . (proteinkinase.biz)
Bidwai AP, Reed JC, Glover CV (1993) "Phosphorylation of calmodulin by the catalytic subunit of casein kinase II is inhibited by the regulatory subunit" Arch. (proteinkinase.biz)
Isoform-specific differences in rates of assembly from catalytic and regulatory subunits" J. Biol. (proteinkinase.biz)
cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein. (systemsbiology.net)
An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. (systemsbiology.net)
activation by cAMP produces two active catalytic monomers and a regulatory dimer. (systemsbiology.net)
A protein complex that possesses protein serine/threonine kinase activity, and contains two catalytic alpha subunits and two regulatory beta subunits. (systemsbiology.net)
CnA also has 3 regulatory domains: a binding domain for its partner subunit CnB, a CaM-binding domain, and an autoinhibitory domain. (pancreapedia.org)
CnB, the regulatory subunit, contains 4 Ca 2+ -binding EF hand motifs that regulate (through a conformational change) the catalytic function of Cn. (pancreapedia.org)

The protein kinase superfamily is mainly composed of the. (systemsbiology.net)

iv) The sequence flanking Ser-133 of EF-1δ completely matches the consensus phosphorylation site for a cellular protein kinase, cdc2, and in vitro kinase assays revealed that purified cdc2 phosphorylates Ser-133 of EF-1δ. (elsevierpure.com)
These results indicate that the conserved protein kinases encoded by herpesviruses and a cellular protein kinase, cdc2, have the ability to target the same amino acid residues for phosphorylation. (elsevierpure.com)

These results strongly suggest that CK II functions primarily in regulating nuclear activities, and that the two catalytic subunits, alpha and alpha', may carry out overlapping functions. (rupress.org)

However, the comparative transcriptome analysis of two spinach accessions with different bolting times identified genome-wide gene expression profiling and large-scale discovery of flowering-related genes from vegetative and reproductive leaves [ 13 ]. (biomedcentral.com)
More than 90% of cases of MDS harbor detectable drive mutations including: DNMT3A, EZH2, RUNX 1, TET 2, IDH 1, IDH 2, TP53, ASXL1, and mutations in genes in coding components involved in the three prime RNA splicing. (standardofcare.com)
Like its homolog Bcl3, IκBζ can regulate the transcription of a set of inflamatory genes through its association with the p50 or p52 subunits of NF-κB. (oncotarget.com)

Benitez MJ, Cochet C, Jimenez JS (2001) "A surface plasmon resonance study of the interactions between the component subunits of protein kinase CK2 and two protein substrates, casein and calmodulin" Mol. (proteinkinase.biz)
Protein kinase CK2 complexes are found in nearly every subcellular compartment, and can phosphorylate many protein substrates in addition to casein. (systemsbiology.net)

Recombinant human bone morphogenetic protein 2 (rh. (researchgate.net)
Recombinant human bone morphogenetic protein 2 (rhBMP2) is a. (researchgate.net)
Human recombinant casein kinase 2 holo enzyme (hCK2 holo) containing alpha (aa1-335) and beta subunits expressed separately in E. coli as non-fusion proteins, purified by several chromatography steps (HiTrapQ, Heparin agarose, Superdex200) and reconstituted to the CK2 alpha2beta2 holoenzyme in the course of purification. (proteinkinase.biz)
ii) A purified chimeric protein consisting of maltose binding protein (MBP) fused to a domain of EF-1δ containing Ser-133 (MBP-EFWt) is specifically phosphorylated in in vitro kinase assays by purified recombinant UL13 fused to glutathione S-transferase (GST) expressed in the baculovirus system. (elsevierpure.com)

The primary sequence of the Cn subunit is highly conserved. (pancreapedia.org)

Casein kinase II is a predominantly nuclear enzyme. (rupress.org)
During mitosis, the bulk of the enzyme was dispersed throughout the cell, though a fraction of all three subunits was associated with the mitotic spindle. (rupress.org)

Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. (wikipedia.org)
This family of proteins comprises two subfamilies that share a DNA-binding and dimerization domain called the Rel homology domain (RHD) [ 4 ] and form homo- or hetero- dimers. (oncotarget.com)

Cac2, a putative subunit of H3.1 deposition complex CAF1, is not required for growth, whereas the expression of the putative ortholog of the H3.3-specific chaperone Hir1 is essential in Tetrahymena . (biomedcentral.com)

Plays a complex role in regulating the basal catalytic activity of the alpha subunit (By similarity). (ac.be)
To increase stability of the holo complex hCK2 holo consist of shortened alpha subunits (aa1-335) and beta subunits. (proteinkinase.biz)
Since CK2 alpha is unstable and prone to degradation, CK2 subunits alpha was shortened at the C-terminus to obtain a stable, crystallizable alpha subunit. (proteinkinase.biz)
The subunit alpha protein activates transcription of the subunit beta gene" J. Biol. (proteinkinase.biz)
A study with the complete set of subunits (alpha, alpha\' and beta), site-directed autophosphorylation mutants and a bicistronically expressed holoenzyme" Eur. (proteinkinase.biz)
Battistutta R, Sarno S, De Moliner E, Marin O, Issinger OG, Zanotti G, Pinna LA (2000) "The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme" Eur. (proteinkinase.biz)
In this study, we have used indirect immunofluorescence microscopy and cell fractionation to study the subcellular distribution of all three subunits of chicken CK II, alpha, alpha', and beta. (rupress.org)
The in vitro treatment of intestinal epithelial cell lines with cytokines resulted in increased CK2 expression and nuclear translocation of its catalytic alpha subunit. (diva-portal.org)
Earlier studies have shown that translation elongation factor 1δ (EF-1δ) is hyperphosphorylated in various mammalian cells infected with representative alpha-, beta-, and gammaherpesviruses and that the modification is mediated by conserved viral protein kinases encoded by herpesviruses, including UL13 of herpes simplex virus type 1 (HSV-1), UL97 of human cytomegalovirus, and BGLF4 of Epstein-Barr virus (EBV). (elsevierpure.com)

Deletion of the catalytic subunit CK2α' in mice results in the male infertility by exhibiting the nuclear abnormalities and apoptosis in the sperm [ 18 , 19 ]. (biomedcentral.com)
Here, we report that during intestinal inflammation, multiple inflammatory cytokines promote the activity of a master regulator of cell proliferation and apoptosis, serine/threonine kinase CK2. (diva-portal.org)

To better understand the function(s) and regulation of this ubiquitous kinase, it is important to know its subcellular distribution. (rupress.org)

aldehyde dehydrogenase 2 family me. (gsea-msigdb.org)

Humans have two copies of chromosome 1, as they do with all of the autosomes , which are the non- sex chromosomes . (wikidoc.org)
It was the last completed chromosome, sequenced two decades after the beginning of the Human Genome Project . (wikidoc.org)

It has two subunits, A and B, each of which has several isoforms and is inhibited by the immunosuppressant drugs FK506 and cyclosporine. (pancreapedia.org)
CnB has two isoforms CnB1 and CnB2. (pancreapedia.org)

We used microarrays to examine the transcriptional responses of the human BE(2)-C neuroblastoma cell line to retinoic acid-induced differentiation and type I IFN stimulation. (gsea-msigdb.org)

This gene encodes a ribosomal protein that is a component of the 60S subunit. (wikipedia.org)

We found that each of the three CK II subunits was located predominantly in the cell nucleus, irrespective of the cell type analyzed or the procedure used for cell fixation. (rupress.org)

In most eukaryotes, two classes of histones have been described: (1) replicative or canonical histones, expressed only during the S phase of the cell cycle, which are assembled onto chromatin in a DNA replication-dependent (RD) manner, and (2) variant histones, which differ in their primary amino acid sequences, are expressed throughout the cell cycle, and can be deposited in a replication-independent (RI) fashion [ 2 ]. (biomedcentral.com)

Biochemical studies based on mechanical enucleation of chicken cells confirmed the predominantly nuclear location of all three CK II subunits. (rupress.org)

Gβ2 was associated with GR/glucocorticoid response elements (GREs) in vivo and suppressed activation function-2-directed transcriptional activity of the GR. We conclude that the Gβγ complex interacts with the GR and suppresses its transcriptional activity by associating with the transcriptional complex formed on GR-responsive promoters. (rupress.org)

E2F transcription factor 2 [Source. (gsea-msigdb.org)

We examined primary chick embryo fibroblasts, virally transformed chicken hepatoma cells, as well as HeLa cells transiently transfected with cDNAs encoding chicken CK II subunits. (rupress.org)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (wikipedia.org)

Herein, we used bioinformatic approaches to analyze the transcriptome datasets from two different accessions Viroflay and Kashan at two vegetative and reproductive stages to reveal novel lncRNAs and the construction of the lncRNA-mRNA co-expression network. (biomedcentral.com)
Finally, immunoblotting experiments were carried out to study the expression of CK II subunits. (rupress.org)
A survey of different adult chicken tissues revealed substantial tissue-specific differences in the levels of CK II protein, but no evidence was obtained for pronounced tissue specificity in the expression of individual CK II subunits. (rupress.org)

cysteine rich hydrophobic domain 2. (gsea-msigdb.org)
COMM domain containing 2 [Source:H. (gsea-msigdb.org)
Two short α-helices form the inhibitory domain and block the catalytic center under basal low Ca 2+ conditions. (pancreapedia.org)
CnAβ also has two splice variants which differ in their C-terminal domain (30). (pancreapedia.org)

Our results raise the possibility that the viral protein kinases mimic cdc2 in infected cells. (elsevierpure.com)

No major differences were detected in the subcellular distributions of individual CK II subunits, and no evidence was obtained for subunit redistributions during interphase of the cell cycle. (rupress.org)

Other Eukaryotes - 2 (source: NCBI BLink). (ntu.edu.sg)

FUNCTIONS IN: protein serine/threonine kinase activity, prote. (riken.jp)
Cn consists of two subunits, CnA and CnB, which form a heterodimer in order to conduct phosphatase activity. (pancreapedia.org)
In a phase 2 clinical investigation, difelikefalin was safe, well tolerated and showed robust analgesic activity for postoperative pain in female patients undergoing laparoscopy, with a significant reduction in post-operative morphine consumption and opioid-related side effects. (ncats.io)

epithelial cell transforming 2 [So. (gsea-msigdb.org)

A dinuclear metal center composed of one iron and one zinc molecule each lies next to a β sandwich on the active site ( Figure 2 ). (pancreapedia.org)

Anatomy22

Organisms17

Chemicals and Drugs142

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes46

Information Science3

CKII2

CAMP-depend1

Regulatory11

Superfamily1

Phosphorylation2

Catalytic subunits1

Genes3

Substrates2

Recombinant4

Highly conserved1

Enzyme2

Proteins2

Putative1

Alpha9

Apoptosis2

Ubiquitous1

Dehydrogenase1

Chromosome2

Isoforms2

Neuroblastoma1

Ribosomal1

Cell nucleus1

Dependent1

Nuclear1

Complex1

TRANSCRIPTION FACTOR1

HeLa1

Consist1

Expression3

Domain4

Cells1

Subcellular1

Eukaryotes1

Activity3

Epithelial1

Sandwich1