• Gene expression was analyzed by measuring beta-galactosidase activity in tissue homogenates or visualizing enzymatic activity or protein production at a cellular level by in situ histochemistry or immunocytochemistry. (nih.gov)
  • The effect of the introduction of plasmids carrying virB (pBN1) or virF (pHW745 and pMYSW6504), and of a Delta hns deletion, in the different strains was evaluated by measuring beta-galactosidase activity, virB transcription, and virB-regulated virulence phenotypes like synthesis of Ipa proteins, contact-mediated hemolysis, and capacity to invade HeLa cells. (uniroma3.it)
  • Find the beta Galactosidase antibody that fits your needs. (thermofisher.com)
  • Affibody molecules are small, robust proteins engineered to bind to a large number of target proteins or peptides with high affinity, imitating monoclonal antibodies, and are therefore a member of the family of antibody mimetics. (wikipedia.org)
  • As with other antibody mimetics, the idea behind developing the Affibody molecule was to apply a combinatorial protein engineering approach on a small and robust protein scaffold. (wikipedia.org)
  • Each beta Galactosidase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our beta Galactosidase polyclonal antibodies and browse our beta Galactosidase monoclonal antibody catalog. (novusbio.com)
  • Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. (ncsu.edu)
  • Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. (ncsu.edu)
  • Antibodies that detect beta Galactosidase can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA and Immunoprecipitation. (thermofisher.com)
  • Our beta Galactosidase polyclonal and monoclonal antibodies are developed in Rabbit, Mouse and Chicken. (thermofisher.com)
  • These antibodies have been verified by Knockdown and Relative expression to confirm specificity to beta Galactosidase. (thermofisher.com)
  • These proteins are characterized by a high rate of extravasation and rapid clearance of non-bound tracer from the circulation, as well as other nonspecific compartments, when compared to antibodies and their fragments Affibody molecules can be used for protein purification, enzyme inhibition, research reagents for protein capture and detection, diagnostic imaging and targeted therapy. (wikipedia.org)
  • Our beta Galactosidase Antibodies can be used in a variety of model species: Bacteria, Mouse. (novusbio.com)
  • Two sets of cDNAs were recovered, and evidence from three independent assays confirmed that both sets encoded articulins: (a) Anti-articulin antibodies recognized a high molecular weight beta-galactosidase (beta-gal) fusion protein expressed in bacteria infected with lambda gt11 cDNA clones. (rupress.org)
  • b) Antibodies generated against the bacterially expressed beta-gal fusion protein identified one or the other articulin in Western blots of Euglena proteins. (rupress.org)
  • We have developed an enzyme-linked immunoassay (ELISA) for measuring antibodies to the 147-carboxyl-terminal amino acids of CENP-B expressed as a beta-galactosidase fusion protein. (bath.ac.uk)
  • Interestingly, it is the "prefusion" form of the "F" fusion protein that has proven to be the most successful immunogenic RSV factor (via its "theta" site) in terms of eliciting neutralizing antibodies and generating promising vaccine trials against both RSV Type A and Type B (2). (genetex.com)
  • For this reason, and to support additional research into basic RSV biology, GeneTex is developing recombinant rabbit monoclonal antibodies against various RSV proteins, focusing first on the RSV nucleoprotein (indicated as NP below). (genetex.com)
  • This study demonstrates the utility of the human beta-PDE promoter for directing the expression of foreign genes to photoreceptors and suggests that the -297 to +53 beta-PDE 5' flanking region fragment may have important implications for therapeutic gene delivery to the visual cells. (nih.gov)
  • Our work deals primarily with the outer membrane proteins, lambda receptor, the product of the lamB gene, and the major outer membrane (porin) proteins 1a and 1b, products of the ompF and ompC genes. (unibas.ch)
  • The structural genes for the porin proteins, 1a and 1b, are regulated at the transcriptional level by the ompB locus. (unibas.ch)
  • Its 15.2 kb genome encodes eleven proteins from ten genes (1). (genetex.com)
  • This suggests that variants of other genes and proteins may also be involved in providing protection. (elifesciences.org)
  • This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. (thermofisher.com)
  • β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (wikidoc.org)
  • [10] The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikidoc.org)
  • We previously demonstrated that 350 bp of the human rod cGMP phosphodiesterase beta-subunit (beta-PDE) gene promoter are sufficient to direct high levels of gene expression in human Y-79 retinoblastoma cells in vitro. (nih.gov)
  • A construct containing the bacterial LacZ gene driven by a fragment of the beta-PDE 5' flanking region (-297 to +53) was used to generate transgenic mice. (nih.gov)
  • Within the retina, the reporter gene was specifically expressed in photoreceptors, consistent with the localization of endogenous beta-PDE. (nih.gov)
  • Photoreceptor-specific reporter gene expression is driven in vivo by a 350-bp segment of the beta-PDE 5' flanking sequence. (nih.gov)
  • In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikidoc.org)
  • The fused gene directed the formation of a large protein showing both L-SD and $\beta$-galactosidase activities. (concordia.ca)
  • This has provided insights into mechanisms of microaerobic gene regulation, and has enabled the development of oxygen-dependent expression systems for high-level synthesis of recombinant proteins. (caltech.edu)
  • HJ1 and HJ4 lysogens produced beta-galactosidase fusion proteins that shared similar epitopes with each other and helveticin J. The intact helveticin J gene (hlv) was isolated by screening a library of L. helveticus chromosomal DNA in lambda EMBL3 with the insert DNA from phage HJ4 as a probe. (ncsu.edu)
  • Using techniques of gene fusion, we have demonstrated that information specifying the cellular location of the lambda receptor is contained within the lamB gene. (unibas.ch)
  • Again using techniques of gene fusion, we have shown that the signal sequence alone is not sufficient to cause export of beta-galactosidase from the cytoplasm. (unibas.ch)
  • The introduction of pBN1 or of the Delta hns deletion in pINV-integrated strains induces temperature regulated expression or temperature-independent expression, respectively, of beta-galactosidase activity and of all virulence phenotypes, while an increase in virF gene dosage does not, in spite of a high-level induction of virB transcription. (uniroma3.it)
  • Moreover, a wild-type has gene placed in trans fully reversed the induction of beta-galactosidase activity due to the Delta hns deletion. (uniroma3.it)
  • At 30 degrees C, the introduction of the high-copy-number plasmid pMYSH6504 (but not of the low copy-number pHW745) or of the Delta hns deletion induces, in strains harboring an autonomously replicating pINV, P-galactosidase activity, virB transcription, and expression of the virulence phenotypes, indicating that, as for HN280/32, the increase in virF gene dosage overcomes the negative regulatory effect of H-NS on virB transcription. (uniroma3.it)
  • Moreover, we have found that virF transcription is finely modulated by temperature and, with a call K-12 strains containing a virF-lacZ gene fusion, by H-NS. (uniroma3.it)
  • The conformation iC3b( Similarly determined to as the photochemical protein) is the Tyrosine by which the phosphorylated carbohydrate senescent( rheumatic) is based from smooth ends in the gene full and formed recently to its 11-cis synthesis single for another turn cblA. (familie-vos.de)
  • Ex vivo adenovirus-mediated gene transfer and immunomodulatory protein production in human cornea. (ox.ac.uk)
  • Subsequently, we examined the expression of a potential therapeutic gene, CTLA-4 Ig fusion protein. (ox.ac.uk)
  • Mitofilin expression was assessed at both mRNA and protein levels, and short hairpin RNA (shRNA)-based gene knockdown was applied for mechanistic experiments. (thno.org)
  • Beta-glucuronidase gene was used as reporter to study the expression characteristic of ProTaSC, showing that the full-length fragment and two 5'-progressive deletion fragments (681 bp and 1096 bp) were able to trigger GUS expression. (chinacrops.org)
  • the tri6 gene, encoding a zinc finger protein involved in the regulation of trichothecene biosynthesis, from Fusarium sporotrichioides (Proctor et al. (justia.com)
  • Using a gene editing tool to selectively substitute some of the building blocks, also known as amino acids, that make up the ATP5G1 protein revealed that improvements to the mitochondria were caused by switching specific amino acids. (elifesciences.org)
  • CMT2A is the most common CMT2 phenotype, most often due to a mutation in the gene that encodes mitochondrial fusion protein mitofusin-2 ( MFN2 ). (msdmanuals.com)
  • In vitro Competition of Natural 'Autographa Californica' Nuclear Polyhedrosis Virus and a Recombinant Expressing a Polyhedrin-Beta Galactosidase Fusion Protein. (epa.gov)
  • Protocols were developed for the use of ORE-based expression systems for regulated, high level synthesis of recombinant proteins in high cell density fermentations. (caltech.edu)
  • 1998). In parallel to the in vivo approach, a bacterial protein expression system was established, leading to the production of recombinant Nph1 and Nph3 for use in future in vitro experiments.Analysis of Nph3 expression in Nph3-KI mice was performed by assaying brain sections for beta-galactosidase activity, based on the co-expression of beta-galactosidase from Nph3-expressing cells. (uni-goettingen.de)
  • Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. (thermofisher.com)
  • By simply decreasing the air supply to cells carrying a VHb-lacZ fusion, [Beta]-galactosidase was expressed to a level of about 10% of total cellular protein in a culture containing 20 g dry cell weight/L. (caltech.edu)
  • lacZ operon fusion either on the autonomously replicating pINV or on the integrated pINV, aas studied. (uniroma3.it)
  • In this study the cell specificity and expression pattern conferred by the short beta-PDE 5' flanking sequence in vivo were examined. (nih.gov)
  • The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikidoc.org)
  • No extensive sequence similarities were found between articulins and other protein sequences in various databanks. (rupress.org)
  • This extra peptide has been termed the signal sequence, and it has been proposed that this signal plays a role in the localization of the extracytoplasmic protein. (unibas.ch)
  • Mutations that alter this sequence prevent export of the lambda receptor protein. (unibas.ch)
  • In this case, the beta-galactosidase protein coding sequence and a flanked cassette were introduced to replace allele. (bios-mep.info)
  • The original Affibody protein scaffold was designed based on the Z domain (the immunoglobulin G binding domain) of protein A. These molecules are the newly developed class of scaffold proteins derived from the randomization of 13 amino acids located in two alpha helices involved in the binding activity of the parent protein domain. (wikipedia.org)
  • Lately, amino acids outside of the binding surface have been substituted in the scaffold to create a surface entirely different from the ancestral protein A domain. (wikipedia.org)
  • The 1,024 amino acids of E. coli β-galactosidase were first sequenced in 1970, [7] and its structure determined twenty-four years later in 1994. (wikidoc.org)
  • In addition to 37% amino acid identity and overall structural similarity, both articulins exhibited a long core domain consisting of over 30 12-amino acid repeats with the consensus VPVPV--V--. Homology plots comparing the same or different articulins revealed larger, less regular repeats in the core domain that coincided with predicted turns in extended beta-sheets. (rupress.org)
  • In the last few years, several laboratories have demonstrated that many proteins (both from eukaryotic and prokaryotic organisms) that are destined to be localized in noncytoplasmic locations initially are synthesized as a precursor with a 15-30 amino acid extension at the NH2-terminal end of the molecule. (unibas.ch)
  • After the degradation of damaged proteins and lipids, amino acids and fatty acids are released into the cytoplasm and recycled for new biosynthesis of cellular components or energy production [ 4 ]. (hindawi.com)
  • Luciferases have become indispensable for the study of fundamental cellular processes including the regulation of transcription and translation, mRNA and protein stability, and nucleic acid/protein interactions. (neb.com)
  • Neurexophilins (Nphs) form a family of proteins that were discovered as components of the alpha-latrotoxin receptor, based on their strong binding to the synaptic membrane proteins alpha-neurexins (Petrenko et al. (uni-goettingen.de)
  • Head-to-tail fusions of Affibody molecules of the same specificity have proven to give avidity effects in target binding, and head-to-tail fusion of Affibody molecules of different specificities makes it possible to get bi- or multi-specific affinity proteins. (wikipedia.org)
  • β-galactosidase synthesis stops when glucose levels are sufficient. (wikidoc.org)
  • Genetic analysis of these mutations has provided evidence for the involvement of the ribosome in the process of protein localization. (unibas.ch)
  • With this genetic system, it should be possible to determine if E coli employs more than a single mechanism for the export of proteins to the outer membrane. (unibas.ch)
  • Examining the genetic code of these resilient cells revealed that Arctic ground squirrels may have a variant form of a protein called ATP5G1. (elifesciences.org)
  • The aim was to generate new binders capable of specific binding to different target proteins with almost good affinity, while retaining the favorable folding and stability properties, and ease of bacterial expression of the parent molecule. (wikipedia.org)
  • Autophagy is a cellular catabolic process that eliminates damaged cell organelles, unfolded proteins, and various intracellular pathogens through lysosomal degradation. (hindawi.com)
  • OMIM 253010), based on a deficiency of different lysosomal enzymes-N-acetylgalactosamine-6-sulfate sulfatase (GALNS) and β-galactosidase, respectively. (medscape.com)
  • A few are evolved beta-galactosidase (EBG), beta-glucosidase , 6-phospho-beta-galactosidase, beta-mannosidase, and lactase-phlorizin hydrolase. (wikidoc.org)
  • BaCell-SysMO 2 Modelling carbon core metabolism in Bacillus subtilis - Exploring the contribution of protein complexes in core carbon and nitrogen metabolism. (fairdomhub.org)
  • Fabry Disease Fabry disease is a sphingolipidosis, an inherited disorder of metabolism, caused by deficiency of alpha-galactosidase A, which causes angiokeratomas, acroparesthesias, corneal opacities, recurrent. (msdmanuals.com)
  • GALNS deficiency induces the accumulation of glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) in multiple tissues, particularly bone, cartilage, heart valves, and cornea, whereas β-galactosidase deficiency induces the accumulation of only KS in those tissues. (medscape.com)
  • Transfection efficiency of chitosan-DNA microparticles into mammalian cells was determined by measuring the b -galactosidase activity in cell lysates. (ualberta.ca)
  • β-galactosidase is an exoglycosidase which hydrolyzes the β- glycosidic bond formed between a galactose and its organic moiety. (wikidoc.org)
  • [3] Beta-gal is inhibited by L-ribose , non-competitive inhibitor iodine , and competitive inhibitors phenylthyl thio-beta-D-galactoside (PETG), D-galactonolactone, isopropyl thio-beta-D-galactoside (IPTG) , and galactose. (wikidoc.org)
  • β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose. (wikidoc.org)
  • Beta-galactose is used in such dairy products as yogurt, sour cream, and some cheeses which are treated with the enzyme to break down any lactose before human consumption. (wikidoc.org)
  • A Nph3-KI targeting vector was designed in a way to result in the generation of a bicistron ic mRNA, encoding for a fusion Nph3-Flag protein, as well as for beta-galactosidase fused to a nuclear localization signal. (uni-goettingen.de)
  • It is a other mRNA cancer which can assist binding protein( %) and a PYD sterol that can assess ASC via a PYD-PYD protein. (familie-vos.de)
  • The two major membrane skeletal proteins (articulins) of Euglena gracilis define a novel class of cytoskeletal proteins. (rupress.org)
  • 60% of the peripheral membrane skeleton of Euglena gracilis consists of equimolar amounts of two proteins (articulins) with M(r)s in SDS gels of 80 and 86 kD. (rupress.org)
  • To understand eventually how these proteins assemble and function in maintaining cell form and membrane integrity we have undertaken a molecular characterization of articulins. (rupress.org)
  • We conclude that the two articulins are related members of a new class of membrane cytoskeletal proteins. (rupress.org)
  • Furthermore, we have shown that this information is capable of directing even a normally cytoplasmic protein, beta-galactosidase, to the outer membrane. (unibas.ch)
  • This has permitted us to extend our studies on outer membrane protein localization to protein 1. (unibas.ch)
  • The autophagophore membrane then elongates and encloses the molecules to be degraded forming an autophagosome, which occurs in two separate conjugation reactions catalyzed by autophagy-related proteins (ATGs). (hindawi.com)
  • Molecularly, in alleviating senescence-associated mitochondrial disorders of BMMSCs, particularly the mitochondrial morphological alterations, RESV upregulates Mitofilin, also known as inner membrane protein of mitochondria (Immt) or Mic60, which is the core component of the mitochondrial contact site and cristae organizing system (MICOS). (thno.org)
  • Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose , and various glycoproteins . (wikidoc.org)
  • [ 13 ] In 1977, Arbisser et al reported a similar case with mild Morquio A syndrome-like symptoms resulting from a deficiency of β-galactosidase, described as MPS IVB. (medscape.com)
  • 1993). Previous studies have shown that neurexophilins are secreted proteins of small molecular weight with structural features that resemble those of neuropeptides, suggesting a possible signaling function mediated by alpha-neurexins (Petrenko et al. (uni-goettingen.de)
  • Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (wikidoc.org)
  • Studies in this area led to the identification of multiple bioluminescent proteins and enzyme systems, some of which have found direct applications in biological research (2). (neb.com)
  • This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
  • In general, autophagy degrades long-lived damaged intracellular proteins, in contrast to the ubiquitin-proteasome system, which controls the degradation of short-lived proteins [ 2 ]. (hindawi.com)
  • Affibody molecules are based on a three-helix bundle domain, which can be expressed in soluble and proteolytically stable forms in various host cells on its own or via fusion with other protein partners. (wikipedia.org)
  • This method revealed the Nph3 expression pattern with high accuracy and sensitivity, suggesting a novel approach for the detailed in vivo identification of cells that express a particular protein. (uni-goettingen.de)
  • These findings provide the first evidence of a protein variant that is responsible for protecting cells during the metabolic stress conditions caused by hibernation. (elifesciences.org)
  • Hence, any substantial structural perturbation due to salt, pH, alcohol, or heat treatment leads to irreversible changes in protein structure, even if a simulated physiological environment is restored. (ncsu.edu)
  • We also establish that muscle activity must be sustained above native levels for calcineurin-dependent dephosphorylation of MEF2A and MEF2D to be transduced into an increase in MEF2 transcriptional function, suggesting that calcineurin cooperates with other activity-linked events to signal via these proteins. (duke.edu)
  • 2009). At E9.0, beta-galactosidase activity was detected in the sinus venosus of the developing heart (Fig. 2A). (bios-mep.info)
  • At 10.5 and E11.5, Robust expression of beta-galactosidase activity persisted in the SAN and surrounding superior vena cava tissue (Fig. 2BCD). (bios-mep.info)
  • We observed beta-galactosidase activity in the SAN region and tissue on top of the ventricular septum in the atrioventricular junction at E18.5 (Fig. 2E) as we predicted from previous report (Blaschke et al. (bios-mep.info)
  • In addition, beta-galactosidase activity was observed in the proximal region from the limb, the anterior part. (bios-mep.info)
  • Specific Affibody molecules binding a desired target protein can be "fished out" from pools (libraries) containing billions of different variants, using phage display. (wikipedia.org)
  • β-galactosidase can catalyze two different reactions in organisms. (wikidoc.org)
  • Distinct classes of RNA catalyse biochemical reactions (ribozymes), although the large subunit rRNA and RNaseP ribozymes common to all organisms require protein partners to function [ 1 ]. (biomedcentral.com)
  • proteins from main endothelium-derived Defects is that the proteasome-mediated kinase binds a direct reductase for polyphosphate that is the trimeric sestrin of IL-1beta and IL-18( Rathinam et al. (familie-vos.de)
  • Beta-galactosidase has many homologues based on similar sequences. (wikidoc.org)
  • The intensity of expression of each reporter protein is measured in the absence of several transcriptional regulators that compete for the RNA polymerase. (fairdomhub.org)
  • ORF2 could encode an 11,808-Da protein. (ncsu.edu)
  • ORF3 could encode a 37,511-Da protein. (ncsu.edu)
  • Swaisgood proposed the principle of physical isolation of nascent folding polypeptide chains and has shown that even proteins that cannot be refolded in solution to give biologically active structure can be refolded to the native state if the folding molecules are physically isolated by immobilization. (ncsu.edu)
  • The digital cleavage of electron against excitatory proteins requires an Interleukin-1 factor of binds to the pathology of inositol-1,4,5-trisphosphate. (familie-vos.de)
  • A site for site-specific conjugation is facilitated by introduction of a single cysteine at a desired position, therefore this engineered protein can be used to conjugate to radionuclides such as technetium-99m and indium-111 to visualize receptor-overexpressing tumors. (wikipedia.org)
  • During elongation and maturation, the phagophore encapsulates damaged proteins and cell organelles. (hindawi.com)
  • Competence for DNA uptake requires expressing DNA uptake proteins on the cell surface, so it's a logical target for attack by the host immune system, and thus perhaps for phase variation. (fieldofscience.com)
  • This is the first three-dimensional structure of a glycosylated module of fibronectin, and provides insight into the possible role of the glycosylation in protein stability, protease resistance and modulation of collagen binding. (embl.de)
  • BORDEN SYMPOSIUM ON STABILITY OF MILK-PROTEINS IN DAIRY FOODS. (ncsu.edu)
  • The three-dimensional structure of proteins exhibits marginal stability even under physiological conditions. (ncsu.edu)
  • keratan processes in these shares presentation G-protein displaced complexes that are anaerobic C which well is the co-ligation of Ca2+ from the C-terminal enzymes. (familie-vos.de)
  • Three independently derived transgenic lines were generated carrying the -297 to +53 beta-PDE 5' flanking region fragment. (nih.gov)
  • They tolerate modification and are independently folding when incorporated into fusion proteins. (wikipedia.org)
  • Nucleolytic ribozymes (hereafter referred to as ribozymes for short) are small RNAs (less than 200 nt) that function independently of proteins, of which nine classes are known to exist (hammerhead, hairpin, Varkud satellite, hepatitis delta virus (HDV), glmS , twister, twister-sister, pistol and hatchet) each with distinctive folding patterns. (biomedcentral.com)
  • Following infection of human corneas with adenoviral vectors encoding CTLA-4 Ig protein, high levels of the fusion protein were detected in corneal culture supernatants for up to 28 days. (ox.ac.uk)
  • For a selected set of transcriptional regulators native proteins are fusion-labelled. (fairdomhub.org)
  • The effect of dialysis on shear stress and shear strain at failure of whey protein concentrate/isolate gels. (ncsu.edu)
  • Though the two major RSV antigenic subgroups, designated "A" and "B", display differences in multiple proteins, the primary antigenic determinant is the "G" attachment glycoprotein. (genetex.com)