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beta-GalactosidaseGalactosidasesalpha-GalactosidaseComplement C3cCerebrosidesLactoseGalactoseAcridine Orangealpha-L-FucosidaseFabry DiseaseNigericinGlycoside HydrolasesGlycosidesCell AgingHistocytochemistryInterleukin-1betaEscherichia colibeta 2-MicroglobulinMolecular Sequence DataReceptors, Adrenergic, betaIntegrin beta3

• Definitions
• Wikipedia
• Web
• Articles
• FAQ

• include

• These enzymes also include an immunoglobulin-like beta-sandwich domain. (wikipedia.org)

• domains

• The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. (wikipedia.org)

• lacZ gene

• A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase. (openwetware.org)
• We offer two ways to detect LacZ gene activity: a colorimetric beta-galactosidase staining kit and a chemiluminescent beta-galactosidase assay. (clontech.com)
• In this kit, beta-galactosidase (the product of the LacZ gene) catalyzes the hydrolysis of X-gal to produce a blue color that is easily visualized. (clontech.com)
• Beta-galactosidase is the product of the LacZ gene. (clontech.com)
• We offer three beta-gal mammalian reporter vectors containing the LacZ gene. (clontech.com)
• β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. (wikipedia.org)
• The bacterial lacZ gene encodes a beta-galactosidase enzyme. (wikipedia.org)

• lactase

• β-galactosidase, also called lactase, beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. (wikipedia.org)
• A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase-phlorizin hydrolase. (wikipedia.org)
• Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (wikipedia.org)

• Mutations

• Mutations in some of the codons of the N-terminal 60aa or C-terminal 100aa results in an inactive, dimeric β-galactosidase. (openwetware.org)

• enzymes

• This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. (ebi.ac.uk)
• These enzymes also include an immunoglobulin-like beta-sandwich domain. (wikipedia.org)

• assays

• following the observation that when beta-galactosidase assays were carried out at pH 6.0, only cells in senescence state develop staining. (wikipedia.org)

• lactose intolerance

• In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikipedia.org)

• catalyzes

• Senescence-associated beta-galactosidase (SA-β-gal or SABG) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides only in senescent cells. (wikipedia.org)

• sequences

• Beta-galactosidase has many homologues based on similar sequences. (wikipedia.org)

• glucose

• β-galactosidase synthesis stops when glucose levels are sufficient. (wikipedia.org)

• yield

• Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. (openwetware.org)

• blue

• The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β-galactosidase to form 5-bromo-4-chloro-indoxyl, which then spontaneously dimerizes and oxidizes to form a bright blue insoluble pigment 5,5'-dibromo-4,4'-dichloro-indigo. (wikipedia.org)
• Blue colonies therefore show that they may contain a vector with an uninterrupted lacZα (therefore no insert), while white colonies, where X-gal is not hydrolyzed, indicate the presence of an insert in lacZα which disrupts the formation of an active β-galactosidase. (wikipedia.org)

• domain

• The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. (wikipedia.org)