The covalent amide bond is then formed with the secondary amine of the coupling enzyme, respectively the amine group of the support (Staros et al. (springeropen.com)
Activity3
Various coupling parameters like coupling time, temperature, pH and protein density was optimized and the esterolytic activity of trypsin was assessed using assay and the optimized conditions were selected to develop stable, non-leachable immobilized trypsin. (actascientific.com)
The widespread use of artificially immobilized enzymes began with the advent of a simple and straightforward method resulting in high efficiency of binding and the preservation of most of the activity. (actascientific.com)
As multistep immobilization process yields less activity of the enzyme, one step covalent immobilization of enzymes onto matrix such as CNBr activated sepharose would yield better immobilization and improved enzyme activity. (actascientific.com)
Produce1
To reduce energy and enzyme costs and produce continuously defined hydrolysates, the enzymes need to be stabilized. (springeropen.com)
Specific4
The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. (springeropen.com)
2012 ). When using enzymes for digesting food proteins, an enzyme specific peptide composition, the so-called peptide fingerprint is formed. (springeropen.com)
Utilizing certain enzymes, with specific catalytic reactions, in making synthetic micro/nanoparticles such as carbon nanotubes5, carbon fibers, and microtubes13 can provide a bubble-production-based propulsion system that drives the nanoparticle via self-diffusion mechanism14. (fanfanbike.com)
Based on this opinion, biocompatible polymers with specific functional groups that degrade with a particular enzyme can be good candidates for use in the synthesis of nanomotors' shell. (fanfanbike.com)