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  • CREB
  • Moreover, by integrating co-expression with transcription factor binding analysis, we showed the TS-associated transcriptional changes were predicted to be co-regulated by calcium-dependent transcriptional regulators, including NFAT, MEF2, CREB, and FOXO, thus providing a mechanism by which altered Ca 2+ signaling in TS patients leads to the observed molecular dysregulation. (biomedcentral.com)
  • We identified the major determinants of SARE as a unique cluster of neuronal activity-dependent cis -regulatory elements consisting of closely localized binding sites for CREB, MEF2, and SRF. (pnas.org)
  • Studies of the transcriptional activation mechanisms leading to upregulation of the immediate early genes c- fos , zif 268, and of the brain-derived neurotrophic factor (BDNF) indicated that transcription factors such as the Ca 2+ /cAMP-response element binding protein (CREB) and the serum response factor (SRF) may play a privileged role as key neuronal activity sensors in the nucleus ( 10 - 12 ). (pnas.org)
  • In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). (genecards.org)
  • gene transcription
  • Further, while HDAC inhibitors are known alter chromatin structure resulting in changes in gene transcription, understanding the exact mechanisms responsible for the preclinical efficacy of these compounds remains a challenge. (mdpi.com)
  • Cocaine is a highly addictive drug that exerts its effects by increasing the levels of released dopamine in the striatum, followed by stable changes in gene transcription, mRNA translation, and metabolism within medium spiny neurons in the striatum. (rupress.org)
  • endogenous
  • A local reduction in cortical GABAergic synapses after a loss of endogenous brain-derived neurotrophic factor, as revealed by single-cell gene knock-out method. (semanticscholar.org)
  • This single element, when positioned immediately upstream of a minimal promoter, was necessary and sufficient to replicate crucial properties of endogenous Arc / Arg-3.1' s transcriptional regulation, including rapid onset of transcription triggered by synaptic activity and low basal expression during synaptic inactivity. (pnas.org)
  • Luciferase activity in transgenic cell lines was induced in response to ionomycin-mediated rise of intracellular calcium levels, treatment with HDAC inhibitors and by over-expression of transcription factors known to increase BDNF expression, indicating that transcription of the transgenic reporter is regulated similarly to the endogenous BDNF gene. (beds.ac.uk)
  • 1999
  • MEF2 family members A-D bind as homodimers and heterodimers to the DNA consensus sequence CTA(A/T) 4 TA(G/A) ( Naya and Olson, 1999 ). (jneurosci.org)
  • PTEN
  • Correlation studies depicted two distinct groups of soft tissue sarcomas: one in which MEF2 repression correlates with PTEN downregulation and a second group in which MEF2 repression correlates with HDAC4 levels. (nih.gov)
  • serum
  • We have now developed a pipeline and the analytical framework to integrate the single-cell analysis of immune signaling networks by mass cytometry and proteomic profiling of secreted serum factors with the precise phenotyping of pregnancy-related clinical outcomes. (stanford.edu)
  • regulation
  • We employed transcription factor binding site enrichment analysis to assess whether TS associated co-expression changes reflect calcium-dependent co-regulation. (biomedcentral.com)
  • Since BDNF is associated with several nervous system disorders it would be beneficial to have cellular reporter system for studying its expression regulation. (beds.ac.uk)
  • nuclear factor
  • However, several targets of ERK5 have been identified, including nuclear factor κB (NFκB), c-myc, and cyclin D, all of which are potential regulators of proliferation ( Wang and Tournier, 2006 ). (rupress.org)
  • NFAT
  • This review further highlights the need to characterize better all factors determining the outcome of calcineurin/NFAT signaling in diseases and the downstream targets mediating the beneficial and detrimental effects. (hindawi.com)
  • genes
  • Furthermore, selective inhibition of NFκB at G2-M phases substantially delayed mitotic entry and inhibited transcription of G2-M-specific genes, including cyclin B1, cyclin B2, Plk-1, and cdc25B. (rupress.org)
  • Also involved in the activation of numerous growth factor- and stress-induced genes. (nih.gov)
  • mechanism
  • Together, our findings suggest a shared mechanism of action for the gateway drugs alcohol and nicotine, and reveal a novel mechanism by which environmental factors may alter the epigenetic landscape of the reward system to increase vulnerability to cocaine addiction. (sciencemag.org)
  • reporter
  • Using modified bacterial artificial chromosome (BAC), we generated several transgenic cell lines expressing humanised Renilla luciferase (hRluc)-EGFP fusion reporter gene under the control of rat BDNF gene regulatory sequences (rBDNF-hRluc-EGFP) in HeLa background. (beds.ac.uk)
  • several
  • OP1 is a member of the transforming growth factor-β superfamily, several of whose members possess neuroprotective activity. (ahajournals.org)
  • downstream
  • These analyses illustrate how a systems biology approach based on gene networks can yield insights into the molecular mechanisms of neural development and function, and provide clues as to the functional impact of the downstream effects of Ca 2+ signaling dysregulation on transcription. (biomedcentral.com)