Bcl-2 (B-cell lymphoma 2), encoded in humans by the BCL2 gene, is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis. (wikipedia.org)
The pro-apoptotic proteins in the BCL-2 family, including Bax and Bak, normally act on the mitochondrial membrane to promote permeabilization and release of cytochrome c and ROS, that are important signals in the apoptosis cascade. (wikipedia.org)
In addition, levels of the prosurvival proteins Bcl-2 and Bcl-XL were reduced in podocytes and neurons from cyclin I-deficient mice, and restoration of Bcl-2 or Bcl-XL expression prevented injury-induced apoptosis. (jci.org)
2 Bcl-2 (B-cell lymphoma-2) family of antiapoptotic (bcl-2, bcl-xl, bcl-w and mcl-1) and proapoptotic (bax, bak and bok) proteins are critical regulators of apoptosis in CLL. (haematologica.org)
3 In particular, bcl-2 has emerged as the most important protein in predicting survival in CLL between 11 proteins that are implicated in the control of apoptosis, proliferation and differentiation. (haematologica.org)
Apoptosis factor EI24/PIG8 is a novel endoplasmic reticulum-localized Bcl-2-binding protein which is associated with suppression of breast cancer invasiveness.Zhao X., Ayer R.E., Davis S.L., Ames S.J., Florence B., Torchinsky C., Liou J.S., Shen L., Spanjaard R.A.Cancer Res. (joplink.net)
Background Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. (qedbio.com)
Bcl-2 inhibits caspase acativity either by preventing release of cytochrome c from the mitochondria and/or by binding to apoptosis-activating factor (Apaf-1). (qedbio.com)
We also revealed that miR-143 upregulation inhibited p-JNK (c-Jun N-terminal kinases) and increased p-Bcl2 (B-cell lymphoma 2), contributing to abiraterone acetate-induced apoptosis in PC3-AbiR cells. (jcancer.org)
Therefore, the current study used integrated in vitro and in silico approaches to figure out Amy and Sor's possible synergistic activity in targeting AMPK/mTOR and BCL-2 for anti-angiogenesis and apoptosis cell death in HepG2 cells. (biomedcentral.com)
The goal herein was to determine the effect of macrophage-targeted deletion of Bcl-2 on macrophage apoptosis in atherosclerotic lesions of Apoe-/- mice. (northwestern.edu)
Macrophages from these mice were more susceptible to apoptosis than those from control Bcl WT -LysMCre mice. (northwestern.edu)
Conclusions-Macrophage Bcl-2 plays a protective role against macrophage apoptosis specifically in advanced atherosclerotic lesions of Apoe -/- mice. (northwestern.edu)
The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. (nih.gov)
Proteínas de la membrana codificadas por los GENES BCL-2 y que sirven como potentes inhibidores de la muerte celular por APOPTOSIS. (bvsalud.org)
Lymphoma6
Bcl-2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. (wikipedia.org)
But simultaneous over-expression of Bcl-2 and the proto-oncogene myc may produce aggressive B-cell malignancies including lymphoma. (wikipedia.org)
In follicular lymphoma, a chromosomal translocation commonly occurs between the fourteenth and the eighteenth chromosomes - t(14;18) - which places the Bcl-2 gene from chromosome 18 next to the immunoglobulin heavy chain locus on chromosome 14. (wikipedia.org)
Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma. (rush.edu)
Human B-cell Leukemia/Lymphoma 2 (Bcl-2) CLIA Kit is a CLIA kit against B-cell Leukemia/Lymphoma 2 (Bcl-2). (markelab.com)
Mouse B-Cell Leukemia/Lymphoma 2 (Bcl-2) CLIA Kit is a CLIA kit against B-Cell Leukemia/Lymphoma 2 (Bcl-2). (markelab.com)
Protein10
The over-expression of the anti-apoptotic Bcl-2 protein in lymphocytes alone does not cause cancer. (wikipedia.org)
Bcl-2 is an intracellular membrane protein and resides primarily in the nuclear envelope, outer mitochondrial membrane and endoplasmic reticulum. (bdbiosciences.com)
A synthetic peptide containing amino acids 61-76 of the human Bcl-2 protein was used as immunogen. (bdbiosciences.com)
Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. (bdbiosciences.com)
Furthermore, we found that levels of phosphorylated MEK1/2 and ERK1/2 were decreased in cyclin I-deficient podocytes and that inhibition of MEK1/2 restored Bcl2 and Bcl-XL protein levels. (jci.org)
Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. (biolegend.com)
By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of Bcl-2 activation after treatment. (genmallestore.com)
Many of the genes with expression change like Bcl-2 gene is specifically, coding an anti-apoptotic protein and and therefore classified as an oncogene. (edu.iq)
The protein encoded by this gene binds to Bcl2 protein and is referred to as Bcl2-associated athanogene. (nih.gov)
Specificity2
Specificity This antibody detects endogenous human Bcl-2 only when phosphorylated at serine 70.Accession no. (qedbio.com)
Moreover, simultaneous measurement of both total and phospho-Bcl-2 confirms target specificity of the phosphorylation event. (genmallestore.com)
Antibody3
It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope. (immunologicalsciences.com)
The Muse Bcl-2 Activation Dual Detection Kit includes two directly conjugated antibodies-a phospho-specific anti-phospho-Bcl-2 (Ser70)-Alexa Fluor® 555 and an anti-Bcl-2-PECy5 conjugated antibody-to measure total levels of Bcl-2 expression. (genmallestore.com)
Anti-Bcl-2 antibody [EPR17509] by Abcam, Cat. (lucerna-chem.ch)
Proteins8
BCL-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. (wikipedia.org)
These pro-apoptotic proteins are in turn activated by BH3-only proteins, and are inhibited by the function of BCL-2 and its relative BCL-Xl. (wikipedia.org)
Taken together, these data suggest that a cyclin I-Cdk5 complex forms a critical antiapoptotic factor in terminally differentiated cells that functions via MAPK signaling to modulate levels of the prosurvival proteins Bcl-2 and Bcl-XL. (jci.org)
Moreover, the recently proposed anti-bcl-2 molecules, such as ABT-199, have emphasized the potential role of of bcl-2 family proteins in the context of target therapies. (haematologica.org)
BCL-2 inhibitors work by blocking these proteins allowing cancer cell death to occur. (sparkcures.com)
Proto-Oncogene Proteins c-bcl-2" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
This graph shows the total number of publications written about "Proto-Oncogene Proteins c-bcl-2" by people in this website by year, and whether "Proto-Oncogene Proteins c-bcl-2" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Proto-Oncogene Proteins c-bcl-2" by people in Profiles. (rush.edu)
Immunogen1
Immunogen Peptide sequence that includes phosphorylation site of Serine 70 (R-T-S(p)-P-L) derived from human Bcl-2 and conjugated to KLH. (qedbio.com)
Gene8
Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. (wikipedia.org)
This fusion gene is deregulated, leading to the transcription of excessively high levels of Bcl-2. (wikipedia.org)
The bcl-2 gene was first discovered in t(14:18) follicular lymphomas. (bdbiosciences.com)
Translocation of bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates bcl-2 gene expression, resulting in high levels of bcl-2 mRNA. (bdbiosciences.com)
Amy and Sor cotreatment resulted in the highest glutathione levels and induction of pro-autophagic genes AMPK, HGMB1, ATG5, Beclin 1, and LC3, suppressed the mTOR and BCL2 anti-apoptotic gene. (biomedcentral.com)
Gene Expression Bcl-2 Gene in Cancer of Brest in Iraqi population', Engineering and Technology Journal , 35(2B), pp. 119-125. (edu.iq)
M. Al-Khafaji, H. Gene Expression Bcl-2 Gene in Cancer of Brest in Iraqi population. (edu.iq)
Follicular lymphomas1
Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas. (biolegend.com)
Mitochondrial fusion and fission1
BCL-2 is known to regulate mitochondrial dynamics, and is involved in the regulation of mitochondrial fusion and fission. (wikipedia.org)
Phase 1 study of lenalidomide plus dose-adjusted EPOCH-R in patients with aggressive B-cell lymphomas with deregulated MYC and BCL2. (rush.edu)
Paraffin-embedded sections1
Immunohistochemistry staining of human placenta (paraffin-embedded sections) with anti-BCL2 (Bcl-2/100), 10 μg/ml. (immunologicalsciences.com)
Phosphorylation3
Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. (biolegend.com)
The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. (immunologicalsciences.com)
This two-color kit is designed to detect Bcl-2 pathway activation by measuring Bcl-2 phosphorylation relative to the total Bcl-2 expression in any given cell population. (genmallestore.com)
Inhibitors1
Finally, the recently proposed new therapies employing bcl-2 inhibitors prompted the potential use of bax/bcl-2 ratio to identify patients putatively resistant to these molecules. (haematologica.org)
Flow cytometry1
We investigated bax/bcl-2 ratio by flow cytometry in 502 patients and identified a cut off of 1.50 to correlate bax/bcl-2 ratio with well-established clinical and biological prognosticators. (haematologica.org)
Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. (bdbiosciences.com)
Pathway1
Feng Y, Cao H, Zhao W, Chen L, Wang D, Gao R. miR-143 mediates abiraterone acetate resistance by regulating the JNK/Bcl-2 signaling pathway in prostate cancer. (jcancer.org)
No. 421002), then stained with Bcl-2 (clone 100) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). (biolegend.com)
Expression3
Bcl-2 expression is frequent in small cell lung cancer, accounting for 76% cases in one study. (wikipedia.org)
Some evidence suggests that this may result from abnormal expression of Bcl-2 and increased expression of caspase-3. (wikipedia.org)
Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. (immunologicalsciences.com)
Molecular weight2
The reduced molecular weight of Bcl-2 is 26 kDa. (bdbiosciences.com)
The predicted molecular weight of Bcl-2 is ~26kDa. (qedbio.com)
Mice2
Orthologs (such as Bcl2 in mice) have been identified in numerous mammals for which complete genome data are available. (wikipedia.org)
Methods and Results-Bcl2 flox -LysMCre mice were created as a model of macrophage Bcl-2 deficiency. (northwestern.edu)
Family4
These bands may represent oligomers of Bcl-2, which have been reported to occur with members of the Bcl-2 family during the preparation of nonionic detergent extracts. (bdbiosciences.com)
Nonionic detergents induce dimerization among members of the Bcl-2 family. (bdbiosciences.com)
It forms homodimers or heterodimers with other Bcl-2 family members. (biolegend.com)
In chronic lymphocytic leukemia the balance between the pro-apoptotic and anti-apoptotic members of the bcl-2 family is involved in the pathogenesis, chemorefractoriness and clinical outcome. (haematologica.org)
Sequence1
This peptide sequence differs from that of mouse Bcl-2 and hence 4D7 does not cross-react with mouse. (bdbiosciences.com)
Survival1
In multivariate analysis of progression-free survival and overall survival, bax/bcl-2 was an independent prognostic factor ( P =0.0002 and P =0.002). (haematologica.org)
Study2
A Phase 1b/2 Study of the BCL-2 Inhibitor Venetoclax in Combination With Standard Intensive AML Induction/C. (sparkcures.com)
A Phase 1b/2, Open-Label, Dose Finding, and Expansion Study of the Bcl-2 Inhibitor BGB-11417 in Patients Wi. (sparkcures.com)