• Since Acinetobacter clinical specimens exhibiting high MICs for polymyxins (MIC, 8-32 μg/mL) were recently detected, we searched for the frequency of occurrence of Acinetobacter spp. (cdc.gov)
  • The significance of Acinetobacter isolates from clinical specimens, such as respiratory secretions from intubated patients or specimens from open wounds, is difficult to determine because they often represent colonization. (msdmanuals.com)
  • In this study, we provide the molecular characterization and epidemiology for 12 NDM-1 positive clinical isolates. (who.int)
  • 6. Molecular characterization of isolated CRE from repetitive colonisation surveys to inform IPC. (who.int)
  • A total of 110 clinical isolates of A. baumannii , collected in a recent 2-year period, were tested for carbapenem antibiotic susceptibility, followed by a molecular analysis of carbapenemase genes. (frontiersin.org)
  • Conclusions: We showed that CRAB isolates positive result of carbapenemase-production and carbapenemase resistance genes of bla OXA-23 seem to be dominant in two tertiary care hospitals in Malang and Manado, Indonesia. (ui.ac.id)
  • The impervious outer membrane and environmental exposure to a large pool of resistance genes are considered as selective pressures that cause XDR isolates in these bacteria [ 7 ]. (biomedcentral.com)
  • GBS isolates from patients with invasive diseases during 2006 -2015 were investigated for capsular serotype, multilocus sequence type (ST), antimicrobial susceptibility, and resistance genes. (medworm.com)
  • Whole genome sequences of these isolates were determined and analysed to compute bacterial multilocus sequence types and plasmid replicon types, infer phylogenetic relationships, and identify antimicrobial resistance and virulence genes. (biomedcentral.com)
  • Detection of gyrA and parC Genes in Clinical Acinetobacter Baumannii Isolates. (edu.iq)
  • To evaluate whether the polymyxin B-resistant strains isolates were epidemiologically related, these isolates were molecularly typed by pulsed-field gel electrophoresis (PFGE) as previously described ( 12 ). (cdc.gov)
  • The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. (biomedcentral.com)
  • Pulsed Field Gel Electrophoresis (PFGE) was used to assess the genomic features of the bacterial isolates. (biomedcentral.com)
  • A. baumannii representative clones were selected by pulsed-field gel electrophoresis. (edu.mx)
  • Methods: A. baumannii isolates collected from patient's clinical cultures in two tertiary care hospitals in Malang and Manado were included. (ui.ac.id)
  • Methods: A. baumannii isolates collected from patient{\textquoteright}s clinical cultures in two tertiary care hospitals in Malang and Manado were included. (ui.ac.id)
  • Methods: After confirming all isolates, TA systems abkBA, mqsRA and higBA were identified. (ac.ir)
  • For epidemiological studies, several typing methods have been used to investigate outbreaks caused by A. baumannii . (biomedcentral.com)
  • A. baumannii is developing into multidrug resistant (MDR), extensively drug resistant (XDR), and pandrug resistant (PDR) bacteria, and its adaptation to the environment with drug resistance has previously been reported ( Durante-Mangoni and Zarrilli, 2011 ). (frontiersin.org)
  • The ability of A. baumannii to form biofilms on living and non-living surfaces is an important factor in the persistence of bacteria because it protects them against environmental stress conditions, such as desiccation and exposure to antibiotics and disinfectants, which makes biofilm infections persistent and challenging to treat [ 9 ]. (biomedcentral.com)
  • Molecular approaches such as PFGE that compare the DNA differences of bacteria have been accepted because of establishing the clonal association in many bacteria including A. baumannii isolates [ 10 ]. (biomedcentral.com)
  • In March 2010, a study in a hospital in Mumbai found that most carbapenem-resistant bacteria isolated from patients carried the blaNDM-1 gene. (wikipedia.org)
  • This study showed the possibility of obtaining the appropriate number of blood cultures and characteristic of isolated bacteria in Vietnam in the future. (fujipress.jp)
  • Extended-spectrum beta-lactamase produc- ing Enterobacteriaceae was found in 37.5% (54) isolates and carbapenem resistant bacteria were identified in 27.8% of patients. (who.int)
  • Conclusion High number of resistant microorganisms was isolated, and increased mortality was documented from infections caused by carbapenem-resistant bacteria. (who.int)
  • The quantity of isolated bacteria from the reservoir was lower than from the air/water syringe in 88% of the dental units. (bvsalud.org)
  • A multidisciplinary guideline development group covering clinical infectious diseases, clinical microbiology, clinical pharmacology, infection control, and guideline methodology experts jointly developed the present clinical practice guidelines based on best available scientific evidence to address the clinical issues regarding laboratory testing, antimicrobial therapy, and prevention of CRGNB infections. (bvsalud.org)
  • Susceptibility profiling of the isolates indicated that 100% retained susceptibility to tigecycline while 11/12 (91.7%) were susceptible to colistin. (who.int)
  • Role of Efflux Pumps in Reduced Susceptibility to Tigecycline among Clinical Isolates of Acinetobacter baumannii . (bvsalud.org)
  • The bacterial isolates were consecutively collected between September 1999 and December 2000 from a tertiary Brazilian hospital, where Acinetobacter spp. (cdc.gov)
  • The extract also exhibited antimicrobial properties against Gram-positive and Gram-negative bacterial strains including clinical isolates. (hindawi.com)
  • Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. (mdpi.com)
  • Enterobacter infections do not have a clinical presentation that is specific enough to differentiate them from other acute bacterial infections. (medscape.com)
  • Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important pathogenic bacterium that can cause nosocomial infection in hospitalized patients with various manifestations. (ui.ac.id)
  • As of June 2010[update], there were three reported cases of Enterobacteriaceae isolates bearing this newly described resistance mechanism in the US, the Centers for Disease Control and Prevention (CDC) stated that "All three U.S. isolates were from patients having received recent medical care in India. (wikipedia.org)
  • It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week, and it is a frequent cause of nosocomial infections. (medscape.com)
  • AbstractTo better understand the progression of severe fever with thrombocytopenia syndrome (SFTS), identify early predictors of mortality, and improve the cure rate, the present study aimed to analyze the demographic feature, clinical characteristics, and laboratory parameters of patients with SFTS and to explore the risk factors associated with fatal outcome. (medworm.com)
  • We retrospectively analyzed demographic feature, clinical characteristics, and laboratory parameters of 216 laboratory-confirmed SFTS patients in Shandong province between January 2015 and December 2019. (medworm.com)
  • Clinical characteristics and completeness of ID documentation were compared between patients with and without an AE. (researchgate.net)
  • The clinical characteristics of 33 hospitalised patients with confirmed CPE, including patient-related factors associated with the development of CPE infections, were examined. (biomedcentral.com)
  • No lytic bacteriophages were detected, but S. aureus was isolated from the surroundings of all patients. (mdpi.com)
  • In a retrospective study from January to December 2013, data on 116 non-repetitive positive clinical samples were retrieved from patients' laboratory records. (who.int)
  • Background: Clinical guidelines are systematically developed statements that assist practitioners and patients to make healthcare decisions for specific clinical circumstances. (bvsalud.org)
  • A new study from the Centers for Disease Control and Prevention (CDC) found that a surprising proportion of cases of carbapenem-resistant Enterobacterales (CRE) are found in isolates from patients in the community (CA-CRE). (medscape.com)
  • A. baumannii in our hospital were highly resistant to all antibiotics, including tigecycline, except for minocycline and colistin which are considered the last resort treatment for multidrug-resistant A. baumannii . (who.int)
  • The management of infections due to A. baumannii is difficult because of rapidly developing resistance, however, tigecycline, a glycylcyline antimicrobial, is in use for several years. (ibu.edu.tr)
  • However, the National Committee for Clinical Laboratory Standards (NCCLS) documents do not currently provide interpretative criteria for the testing of polymyxins ( 7 ). (cdc.gov)
  • A. calcoaceticus can be used as an alternative to A. baumannii in the laboratory setting. (wikipedia.org)
  • Clinical Laboratory. (ac.ir)
  • to begin with, first, the clinical samples are sent to the laboratory for acinetobacter bumanni identification through chemical tests, next, the existence of this bacterium is confirmed by the pcr molecular method and then, the antibiotic sensitivity test is run on the samples, followed by their their analysis through spps and chi-square test. (icbcongress.com)
  • Carbapenem-resistant Acinetobacter baumannii (CRAB) presents a serious therapeutic and infection control challenge. (frontiersin.org)
  • Sixty-seven of the 110 isolates (60.9%) were resistant to carbapenems, 80.60% (54/67) of which carried the bla OXA-23 gene. (frontiersin.org)
  • Thus, CC92 A. baumannii carrying OXA-23 is a major drug-resistant strain spreading in China. (frontiersin.org)
  • isolates were considered resistant to polymyxin B (MICs, 8-32 μg/mL) ( 8 , 11 ). (cdc.gov)
  • The polymyxin B-resistant isolates were categorized as susceptible by disk diffusion (100%, very major error). (cdc.gov)
  • Identifications of meropenem-resistant A. baumannii isolates with the vitek2® system results, followed by a sensitivity test using 10 µg imipenem antibiotic disc according to CLSI guidelines to fulfill the criteria as CRAB isolates. (ui.ac.id)
  • Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. (biomedcentral.com)
  • The aim was to evaluate the antimicrobial activity of essential oils-derived volatile compounds against nosocomial pathogens, including representative multidrug-resistant (MDR) Acinetobacter baumannii clinical isolates. (edu.mx)
  • This study underscores the major role of carbapenem-hydrolyzing class D β-lactamases, and in particular the acquired OXA-23, in the dissemination of imipenem-resistant A. baumannii . (biomedcentral.com)
  • Our phenotypic analysis clearly demonstrated that A. baumannii isolates obtained from different hospitals in central Ohio were resistant to all clinically significant antibiotics, including carbapenems (imipenem). (biomedcentral.com)
  • Whole genome sequencing revealed the close relationship of an antibiotic-susceptible chicken isolate from Germany with a multidrug-resistant human clinical isolate from China and additional linkages between livestock isolates and human clinical isolates related to international clonal lineages. (uni-koeln.de)
  • One carbapenem-resistant A. pittii harboured bla OXA-58, while another isolate co-harboured bla OXA-58 and bla IMP-14a . (microbiologyresearch.org)
  • Reduced OMPs were found in carbapenem-resistant and -susceptible A. pittii carrying bla OXA-58 , but were not detected in carbapenem-resistant A. nosocomialis isolates. (microbiologyresearch.org)
  • The isolate was multidrug resistant, but remained susceptible to the carbapenems, amikacin and piperacillin/tazobactam. (microbiologyresearch.org)
  • All CPE isolates carrying either bla OXA-232 or bla NDM-1 were resistant to meropenem, but only 40 from 45 were susceptible to colistin. (biomedcentral.com)
  • About 85% of the colonies isolated from surface samples were resistant to antimicrobials, with 94% of them multidrug resistant. (mdpi.com)
  • The isolates were completely resistant to the second- and third-generation cephalosporins tested as well as carbapenems. (who.int)
  • Acinetobacter baumannii resistant records at Al-Hussein Hospital, King adopted in this study. (who.int)
  • Emergence of Oxacillinases in Environmental Carbapenem-Resistant Acinetobacter baumannii Associated with Clinical Isolates. (cdc.gov)
  • CRGNB isolates are usually extensively drug-resistant or pandrug-resistant, resulting in limited antimicrobial treatment options and high mortality. (bvsalud.org)
  • Hosam is a member of the Infection and Immunity Theme, where he is studying antimicrobial resistant Gram-negative bacilli isolated from the countries of the Gulf Cooperation Council (GCC). (edu.au)
  • Microbiological laboratories should be aware of the unusual carbapenem-resistant but third-generation cephalosporin-susceptible profiles of these IMI-producing isolates. (medscape.com)
  • Multi-center studies should be done to determine the extent of resistant organisms in health facilities throughout the country.epidemiology, and the findings should be factored into clinical decision making and program design for disease prevention, screening, and treatment. (who.int)
  • Over the last decade, nosocomial infections due to Acinetobacter baumannii have been described with an increasing trend towards multidrug resistance, mostly in intensive care units. (biomedcentral.com)
  • In this set of OXA producing isolates, multidrug resistance was bestowed by bla ADC-25 , class 1 integron-borne aminoglycoside modifying enzymes, presence of sense mutations in gyrA / parC and involvement of active efflux (with evidence for the presence of adeB efflux gene). (biomedcentral.com)
  • Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14: results from the Study for Monitoring Antimicrobial Resistance Trends (SMART). (cdc.gov)
  • These isolates were obtained from four local general hospitals in Singapore. (who.int)
  • During the period of August 2010-August 2011, 52 non-duplicate carbapenem non-susceptible clinical isolates from local hospitals were analysed. (who.int)
  • The isolates were submitted from four hospitals that represented 40% of the general hospitals in Singapore. (who.int)
  • The most abundant carbapenemase gene family in the K. pneumoniae isolates (33/39) was bla OXA-232 , with bla NDM-1 additionally identified in 19 of them. (biomedcentral.com)
  • Heparin produced dose-dependent growth inhibition of three of seven S. pneumoniae isolates (complete inhibition at 2500U dose per 200 microL) and one of five H. influenzae isolates (complete inhibition at 7500 U dose per 200 microL), but no inhibition of other isolates. (nih.gov)
  • Therefore, in order to reduce the clonal contamination of MDR A. baumannii environmental and clinical isolates, it is necessary to use strict infection control strategies. (biomedcentral.com)
  • reported the dissemination of MDR OXA-23-producing A. baumannii clones throughout multiple cities in China, but little is known about the molecular mechanisms of resistance to carbapenems in western China. (frontiersin.org)
  • Most of these CRAB isolates (77.62%) were classified as clone complex 92 (CC92), and sequence type (ST) 92 was the most prevalent STs, followed by ST195, ST136, ST843, and ST75. (frontiersin.org)
  • One CRAB isolate of ST195 harbored plasmid pAB52 from a Chinese patient without travel history. (frontiersin.org)
  • The largest number of samples came from sputum and indicates that XDR has also occurred in all CRAB isolates. (ui.ac.id)
  • A national prevention and surveillance system should be prepared to reduce and limiting transmission of CRAB isolates. (ui.ac.id)
  • From all tested compounds, carvacrol had markedly lower MIDs (3.89-48.8 mg/L) against A. baumannii than against the other nosocomial MDR pathogens. (edu.mx)
  • The Infectious Diseases Society of America (IDSA) identified A. baumannii among the top seven pathogens threatening our healthcare-delivery system and as a crucial example of unmet medical need [ 18 ]. (biomedcentral.com)
  • The clinical use of polymyxins has been based on antimicrobial susceptibility results and previous clinical experience. (cdc.gov)
  • We analysed the clinical information, performed antimicrobial susceptibility testing, and conducted molecular epidemiological and genomic analyses on the isolates to identify patterns in the data. (biomedcentral.com)
  • CPE isolates were purified, and antimicrobial susceptibility tests performed. (biomedcentral.com)
  • Antibiotic resistance of the isolates was assessed by the disk diffusion method, and the biofilm formation test was done by the microtiter plate method. (biomedcentral.com)
  • The 98point6 Clinical Quality Assurance team tracks adherence to antibiotic guidelines and reports this data across the company and to partners. (cdc.gov)
  • The SATURN project had as aim to study the impact of antibiotic exposure on AMR with a multidisciplinary approach that bridges molecular, epidemiological, clinical and pharmacological research. (europa.eu)
  • Biofilm production capabilities revealed that 31 (44.9%), and 30 (43.5%) isolates had strong and moderate biofilm producer activity, respectively. (biomedcentral.com)
  • Biofilm production potential of clinical isolates of Acinetobacter baumannii was investigated in this study. (microbiologyjournal.org)
  • The clinical isolates showed varied potential of biofilm production, ranging from a minimum of 1.34 ± 0.28 by a blood isolate to a maximum of 2.02 ± 0.49 by a wound isolate as determined by crystal violet dye binding assay. (microbiologyjournal.org)
  • Biofilm production by the A. baumannii strains were also investigated by growing the strains in presence of different percentage (0-20 %, v/v) of normal human serum (NHS) to determine whether NHS has any effect on biofilm production. (microbiologyjournal.org)
  • Production of biofilm by A. baumannii strains was increased in presence of serum in a concentration dependent manner, reaching a maximum at 20 % concentration. (microbiologyjournal.org)
  • Strains AB-1 (blood isolate) and AB-2 (wound isolate) showed significant increase in BF production in presence of 20 %, while in the case of the strain AB-3 (UTI isolate), there was a partial reduction in biofilm production in presence of serum. (microbiologyjournal.org)
  • Taken together, the findings of this study indicate that clinical A. baumannii isolates exhibit varied biofilm forming potential which is influenced by NHS in a differential manner. (microbiologyjournal.org)
  • Acinetobacter baumannii isolates in Al-Hussein Hospital at King Hussein Medical Centre in Amman, Jordan. (who.int)
  • As a clinical microbiologist he recognized the need to develop more rapid diagnostic tools to aid initial medical management and implement infection control precautions. (edu.au)
  • But in this study, published in the American Journal of Infection Control , of the 12 isolates that underwent whole-genome sequencing, 42% of the CA-CRE isolates carried the carbapenemase gene. (medscape.com)
  • Virulence of selected avian A. baumannii isolates was comparable to that of clinical isolates in the Galleria mellonella infection model. (uni-koeln.de)
  • This study has revealed the genomic features of colonising CPE isolates, focusing on antimicrobial resistance and virulence determinants. (biomedcentral.com)
  • The fact that 5 of the 12 isolates harbored a carbapenemase gene introduces new challenges for controlling spread of CP-CRE. (medscape.com)
  • We investigated the prevalence and mechanisms of carbapenem resistance in A. pittii and A. nosocomialis Thai isolates. (microbiologyresearch.org)
  • its clinical prevalence appears not to be correlated with predominance in the environment, where non-Sg1 strains show higher recovery percentages. (frontiersin.org)
  • The low non-Sg1 sensitivity of the commonly used diagnostic method could be a reason for Sg1 clinical prevalence, however, previous studies link it to an increased resistance to the alternative serum complement pathway, due to variations in the outer-membrane O -antigen segment of the lipopolysaccharide. (frontiersin.org)
  • Distribution of Campylobacter jejuni multilocus sequence types isolated from chickens in Poland. (cdc.gov)
  • The carbapenemases in A. baumannii have belonged to the bla OXA-23- , bla OXA-24- , and bla OXA-58- type class D family of serine β-lactamases and IMP/VIM class B metallo-β-lactamases [ 3 , 4 ]. (biomedcentral.com)
  • The expression level of the abkB gene in persistent isolates showed a sevenfold increase compared with nonpersistent isolates. (ac.ir)
  • The presence of bla OXA-23 in 13% (11/83) and IS Aba1 linked bla OXA-66 in 79.5% (66/83) of clinical isolates was associated with high level imipenem resistance. (biomedcentral.com)
  • the percentages of acinetobacter bumannii isolates in relation to imipenem, meropenem and ciprofloxacin in the dilution serial method are 40.9%, 60% and 77.7%, respectively. (icbcongress.com)
  • Although it appears that the isolates are clonally unrelated, epidemiological links cannot be fully excluded without further research. (who.int)
  • All isolates were susceptible to meropenem and belonged to A. baumannnii ( 4 ) and A. lwoffi ( 1 ) species. (cdc.gov)
  • These isolates were obtained as part of a hospital surveillance programme for carbapenem non-susceptible Enterobacteriaceae. (who.int)
  • Sixteen clinical questions were proposed from the perspective of current clinical practice and translated into research questions using PICO (population, intervention, comparator, and outcomes) format to collect and synthesize relevant evidence to inform corresponding recommendations. (bvsalud.org)
  • Crystalloid Fluid Choice and Clinical Outcomes in Pediatric Sepsis: A Matched Retrospective Cohort Study. (chop.edu)
  • resulting in poor clinical outcomes. (bvsalud.org)
  • Here, we provide a step-by-step protocol to (1) identify whether host genome projects contain reads from associated Wolbachia and (2) isolate/retrieve the Wolbachia reads from the rest of the sequenced material. (bvsalud.org)
  • The isolates were identified to the species level using the BBL Crystal System (Becton Dickinson, Sparks, MD). The susceptibility to polymyxin B and meropenem were tested by disk diffusion and agar dilution techniques according to NCCLS recommendations ( 9 , 10 ). (cdc.gov)