• However, both acute and chronic therapy in vivo with steroids fails to lead to an impairment of subsequent histamine release in vitro. (northwestern.edu)
  • To test whether the failure of in vivo steroid therapy to inhibit subsequent in vitro histamine release was due to the selection of a subpopulation of basophils that responded normally to anti-IgE but were resistant to steroids, the in vitro sensitivity to inhibiton of mediator release by steroids in basophils obtained from normal patients as well as patients receiving chronic steroid therapy was studied. (northwestern.edu)
  • Mit Hilfe von Generationen-übergreifenden Mausmodellen, möglichst in einem translationalen Ansatz durch Kombination mit epidemiologischen Daten ( Plattform Kohorten ) und in-vitro oder ex-vivo Zellkulturmodellen ( Plattform Zellkultur-Modelle , Ernährungsphysiologische Immunologie ), wollen wir die Auswirkungen früher chemischer Expositionen auf die Krankheitsentwicklung im späteren Leben untersuchen und die zugrunde liegenden Mechanismen charakterisieren. (ufz.de)
  • Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro . (rndsystems.com)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • These IgG4 antibodies have the potential to form the basis of a therapeutic drug candidate exhibiting strong potency and efficacy in vitro , ex vivo , and in vivo . (blackradionetwork.com)
  • Thus, dissociation of in vivo CBH and in vitro basophil sensitization was demonstrated. (utmb.edu)
  • Allergy diagnostics starts with evaluation of the patient's medical history followed by in vivo and/or in vitro testing. (kth.se)
  • The aims of this thesis were to improve the existing BAT using microfluidic techniques to enable fast and cheap point-ofcare (POC) diagnostics as well as to further study basophil adhesion and activation for a better understanding of basophil behavior and regulation both in vivo and in vitro in a microfluidic chip. (kth.se)
  • Usefulness of In Vivo and In Vitro Diagn. (istanbul.edu.tr)
  • Rilzabrutinib demonstrated dose-dependent improvement of clinical scores and joint pathology in a rat model of collagen-induced arthritis and demonstrated reductions in autoantibody-mediated FcγR signaling in vitro and in vivo, with blockade of rat Arthus reaction, kidney protection in mouse Ab-induced nephritis, and reduction in platelet loss in mouse immune thrombocytopenia. (aai.org)
  • IL-4 induces Raldh2 expression in bone marrow-derived macrophages in vitro and peritoneal macrophages in vivo . (prolekarniky.cz)
  • Our approach is based on the Basophil Activation Test (BAT), which measures the activation of basophils in whole blood after stimulation with specific food allergens ex vivo. (stanford.edu)
  • The recombinant peptide corresponding to residues 301-376 at the junction of constant regions 2 and 3 of the human IgE ε chain blocked the in vivo passive sensitization of human skin mast cells and in vitro sensitization of human basophil granulocytes with human IgE antibodies. (johnshopkins.edu)
  • However, although eosinophilia commonly accompanies helminthic infections and eosinophils are toxic to helminths in vitro, there is no direct evidence that they kill parasites in vivo. (msdmanuals.com)
  • Blood sample analyses included IgE against dog dander, dog allergen molecules, other airborne and food allergens, basophil activation and white blood cell counts. (ersjournals.com)
  • A cluster of these children with enhanced CST1 expression showed lower forced expiratory volume in 1 s, increased bronchial hyperreactivity, pronounced eosinophilia and higher basophil allergen threshold sensitivity compared with other children sensitised to dog dander. (ersjournals.com)
  • Cross-linking of FcεRI and IgE complexes with allergen induces basophil degranulation and release of inflammatory chemical mediators, leading to clinical symptoms. (nottingham.ac.uk)
  • The allergen-specific IgE in patients' sera might not always lead to FcεRI cross-linking on mast cells or basophils, resulting in no related clinical symptoms, as observed in some food allergies. (nottingham.ac.uk)
  • Allergen extract is intended for in vitro stimulation of basophilic granulocytes, which is IgE-mediated on cellular level, to determine a measure of basophil activation by means of flow cytometry (BAT) in human whole blood sample. (exbio.cz)
  • Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). (kth.se)
  • In paper II, we further investigated whether it was possible to detect a dose-dependent allergen activation for basophils captured in a microfluidic chip. (kth.se)
  • We detected a significant difference in CD63-expression between the negative control and allergen-activated basophils from allergic patients but no difference between the negative control and the non-relevant allergen (an allergen to which the patient had no IgE antibodies). (kth.se)
  • Lirentelimab inhibits mast cells' IgE-mediated degranulation and de novo synthesis of prostaglandin D2 in vitro. (wikipedia.org)
  • Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
  • IgE-dependent (anti-IgE) basophil degranulation after crosslinking of adhesion molecules, to mimic adhesion before transmigration into tissue, showed a significant decrease in CD63-expression compared to anti-IgE activation, which indicate a regulatory function. (kth.se)
  • Cytokine stimulation followed by IgE-independent (fMLP) basophil degranulation on the other hand showed a significantly increased CD63-expression compared to non-primed fMLP activation. (kth.se)
  • We investigated the antigen-specific sensitization of basophils in the peripheral circulation of guinea pigs using an in vitro histamine release assay. (utmb.edu)
  • In paper I, we developed a novel microfluidic immunoaffinity-based basophil activation test (miBAT) assay to investigate whether it was possible to capture and activate basophils from whole blood in a microfluidic chip. (kth.se)
  • The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics. (lu.se)
  • According to the American National Cancer Institute, crude plant extracts are considered cytotoxic in an in vitro assay when concentrations 20 μg/mL and below produce 50% inhibition of tumor cells, after an exposure time of 48 hours. (ru.ac.za)
  • To investigate the mechanisms of anaphylactoid reactions to radiocontrast media, in vitro mediator release induced by three iodinated contrast agents was examined using peripheral blood basophils and mast cells purified from human lung parenchyma, heart, and skin tissues. (johnshopkins.edu)
  • In conclusion, we have developed a novel microfluidic-based technique (miBAT) able to detect basophil activation (CD63-expression) using allergens in allergic patients. (kth.se)
  • In vitro analysis of birch-pollen-associated food allergy by use of recombinant allergens in the basophil activation test. (comparedatabase.org)
  • Accurate in vitro methods that are efficient and easy to use to identify offending food allergens are lacking. (stanford.edu)
  • In addition to potent and selective BTK enzyme and cellular activity, rilzabrutinib inhibited activation and inflammatory activities of B cells and innate cells such as macrophages, basophils, mast cells, and neutrophils, without cell death (in human and rodent assay systems). (aai.org)
  • Finally, the generation of short-lived plasmablasts MG-132 in vitro during natural or postimmune B-cell responses to TI antigens such as microbial carbohydrates and glycolipids involves multiple subsets of myeloid and plasmacytoid DCs, FDCs, epithelial cells, neutrophils, basophils, and mast cells, particularly in the MZ of the spleen and at mucosal sites. (pdgfrsignals.com)
  • Eosinophils are granulocytes (white blood cells that contain granules in their cytoplasm) derived from the same progenitor cells as monocytes-macrophages, neutrophils, and basophils. (msdmanuals.com)
  • Immunologically, AD is driven by TSLP-secretion by keratinocytes and epithelial cells which modulate dendritic cell (DC) and basophil function to preferentially induce a Th2 immune response. (springeropen.com)
  • Zinc chloride was a competitive antagonist of the action of Ca2+ in histamine secretion induced by anti-IgE with a dissociation constant (Kd) of about 10(-5) M in both the basophil and mast cell systems. (nih.gov)
  • Select monoclonal IgG4 antibodies, re-engineered from human IgE antibodies, were able to inhibit allergic plasma IgE from binding recombinant Ara h 2 in vitro, and also inhibit whole peanut-mediated mast cell and basophil activation. (blackradionetwork.com)
  • However, they did not induce the de novo synthesis of leukotriene C 4 or prostaglandin D 2 from human basophils or any type of mast cell examined. (johnshopkins.edu)
  • We have previously shown that physiological concentrations of zinc (congruent to 7 X 10(-6) M) inhibit the release of histamine from human basophil leukocytes (Marone et al. (nih.gov)
  • Thus physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells, presumably by blocking Ca2+ uptake induced by anti-IgE activation. (nih.gov)
  • Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
  • BACKGROUND: IL-5 plays a central role in eosinophil and basophil differentiation, exerting its effects through the IL-5 receptor (IL-5Ralpha). (dadamo.com)
  • Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)
  • OBJECTIVE: Because all-trans retinoic acid (ATRA) is known to modulate some aspects of hemopoietic differentiation, we examined the effects of ATRA on eosinophil-basophil differentiation and IL-5Ralpha expression. (dadamo.com)
  • RESULTS: In semisolid methylcellulose cultures of normal human bone marrow, ATRA (10(-6) mol/L) selectively suppressed eosinophil-basophil colony-forming units but had no effect on granulocyte-macrophage colony-forming units. (dadamo.com)
  • Similarly, ATRA (10(-6) mol/L) inhibited eosinophil-basophil differentiation of cord blood CD34(+) cells in liquid culture, whereas neutrophil differentiation proceeded without impediment. (dadamo.com)
  • CONCLUSIONS: These findings indicate that retinoids can differentially regulate expression of IL-5Ralpha, but not GM-CSF receptor, and that these effects have functional consequences in vitro on eosinophil and basophil differentiation. (dadamo.com)
  • At a bee venom concentration of 100 ng/mL, the mean proportion of basophils expressing CD63 was 56% in reactors and 13.2% in nonreactors (P=.0321). (jiaci.org)
  • Basophils of non-stimulated human peripheral whole blood of a proven allergic donor in the presence of stimulation buffer stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit. (exbio.cz)
  • Separation of CD63 positive basophils (90.0 %) from CD63 negative basophils (10.0 %) in flow cytometry analysis (surface staining) of Soymilk (f200) extract stimulated human peripheral whole blood of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit. (exbio.cz)
  • The captured basophils were activated using an anti-FceRI antibody and the basophil identification marker CD203c and the activation marker CD63 were detected using fluorescence microscopy. (kth.se)
  • Basophils did not upregulate CD63 after passage through a microfluidic chip, but there was a slight but significant activation after crosslinking of CD203c, which is the surface marker used for basophil-specific capture in miBAT, giving one potential factor for the background activation. (kth.se)
  • In these experiments we compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung. (nih.gov)
  • These results show that different contrast media can differ in their ability to release mediators from enriched preparations of human basophils and mast cells. (johnshopkins.edu)
  • The three contrast agents examined act on basophils and mast cells as incomplete secretagogues, causing the release of preformed mediators, but not the de novo synthesis of chemical mediators. (johnshopkins.edu)
  • A detailed clinical history, skin test (ST), and single-blind placebo-controlled drug provocation test (SBPCDPT), as well as basophil activation test (BAT) and lymphocyte transformation test (LTT) were performed with the culprit and alternative quinolones including ciprofloxacin (CFX), moxifloxacin (MFX), levofloxacin (LFX), ofloxacin (OFX), and GFX. (istanbul.edu.tr)
  • Based on in vitro tests, the strains selected for the study prevent the growth of bacteria associated with genitourinary tract infections, and their antibiotic sensitivity profile meets the requirements of the European Food Safety Authority. (who.int)
  • Three iodinated contrast agents, sodium and meglumine salts of ioxaglic acid, sodium and meglumine salts of ioxithalamic acid, and ioversol, were incubated with basophils purified from peripheral blood and human mast cells isolated and purified from different anatomical sites. (johnshopkins.edu)
  • Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. (rndsystems.com)
  • We investigated usefulness of the basophil activation test (BAT) in predicting the outcome of sting challenge in bee venom allergic patients after VIT. (jiaci.org)
  • However, at a concentration of 1000 ng/mL, no significant differences in basophil activation were observed between reactors and nonreactors. (jiaci.org)
  • Basophil activation test. (jiaci.org)
  • The platform in development relies upon the use of a humanised rat basophilic leukaemia (RBL) cell line RBL-703/21 and different methods to measure the levels of basophil activation. (nottingham.ac.uk)
  • Basophil activation test (BAT) can help diagnose immediate reactions to beta-lactams, although controversy exists regarding the best activation marker. (bvsalud.org)
  • In paper III, we studied basophil adhesion and activation to better understand both basophil function as well as the effect that basophil capture and stimulation in a microfluidic chip has on the cell. (kth.se)
  • The basophil capture in a microfluidic chip could potentially mimic basophil adhesion to the endothelium and was therefore of interest due to the elevated background activation seen in unstimulated basophils captured in a microfluidic chip, reported in paper I and II. (kth.se)
  • Animals were primed for cutaneous basophil hypersensitivity (CBH) by intradermal injection of either keyhole limpet hemocyanin (KLH) or ovalbumin (OA) in saline. (utmb.edu)
  • Grant, J. A. / Dissociation between cutaneous basophil hypersensitivity and basophil sensitization . (utmb.edu)
  • Analyzing 818 ligand-receptor interaction pairs within and between cell lineages, we identify broadly interacting cells, including AT2, innate lymphocytes (ILCs), and basophils. (nih.gov)
  • Previous studies have shown that in vitro culture of human basophils for 24 h with physiologic concentrations of glucocorticoids leads to a pronounced inhibiton of the subsequent release of histamine or leukotrienes when the cells are challenged with anti-IgE. (northwestern.edu)
  • Preincubation (5 min, 37 degrees C) of human basophils and lung mast cells with zinc chloride (10(-6)-3 X 10(-5) M) caused dose-related inhibition of histamine and peptide leukotriene C4 (LTC4) release induced by anti-IgE. (nih.gov)
  • title{Protein Tyrosine Phosphatase Inhibition Mimics Fc$\varepsilon$RI-induced Anergy and Reveals the Immunomodulatory Role of Hydrogen Peroxide as a Signaling Molecule in Human Basophils\thanksref{grant}} %Really struggling with a title that captures this paper -- probably a bad sign! (openwetware.org)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • Effect of acidic and basic isoferritins on in vitro growth of human granulocyte-monocyte progenitors. (unito.it)
  • Cross-linking of IgE by anti-IgE induced the release of leukotriene C 4 or prostaglandin D 2 from human basophils or mast cells. (johnshopkins.edu)
  • Mannitol, an osmotic stimulus, induced the release of histamine from human basophils, but to a lesser extent from mast cells. (johnshopkins.edu)
  • Using interleukin (IL)-33 receptor knockout mice and in vitro experiments, we show that basophils establish a lung-specific function imprinted by IL-33 and granulocyte-macrophage colony-stimulating factor (GM-CSF), characterized by unique signaling of cytokines and growth factors important for stromal, epithelial, and myeloid cell fates. (nih.gov)
  • Interleukin 1 releases histamine from manual labourercuffs basophils and mast apartments in vitro. (upb.ro)
  • Antibody-depletion strategies, diphtheria toxin-mediated selective depletion of basophils, and co-culture studies show that lung resident basophils are important regulators of alveolar macrophage development and function. (nih.gov)
  • Basophils isolated from these animals 7 days after sensitization did not release histamine to the specific antigen in vitro. (utmb.edu)
  • When animals were immunized via the footpad by emulsification of antigen in incomplete Freund's adjuvant, basophil sensitization (i.e., histamine release) either occurred earlier (day 7, KLH) or at lower antigen concentrations (OA). (utmb.edu)
  • In order to mimic the allergic response and generate an in vitro diagnostic device to address these issues, a basophil-microarray platform that couples the diversity of a protein array with the biological output of basophilic cells is being developed. (nottingham.ac.uk)
  • Two effector cells involved in allergic disease are mast cells and basophils, where basophils are more readily available in blood and therefore of great interest when studying allergy. (kth.se)
  • Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. (kth.se)
  • At present, no reliable in vitro test is available to monitor the success of specific venom immunotherapy (VIT) in preventing insect venom anaphylaxis. (jiaci.org)
  • Differences in the in vitro growth pattern of fresh and cryopreserved granulo-monopoietic precursors. (unito.it)
  • An in vitro studAn in vitro study of basophil production in chronic myeloproliferative disorders. (unito.it)
  • In vitro diagnostics" is a term for a broad industry encompassing everything from benchtop or larger bioanalytical instrumentation applications such as protein analysis and next generation sequencing to point of care devices. (technologynetworks.com)
  • WBC and Basophils (BASO) are treated with an acidic lyse, that lyses RBC and WBC, but not BASO. (crlcorp.com)
  • In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. (elifesciences.org)
  • basophils b. cd6 and cd6 regu- idiotope lator cells. (stonecottagegardens.com)