• When methylene blue is "polychromed" (oxidized in solution or "ripened" by fungal metabolism, as originally noted in the thesis of Dr. D. L. Romanowsky in the 1890s), it gets serially demethylated and forms all the tri-, di-, mono- and non-methyl intermediates, which are Azure B, Azure A, Azure C, and thionine, respectively. (wikipedia.org)
  • On the other hand, if methylene blue is used it might help to give the normal look of neutrophil granules and may also enhance the staining of nucleoli and polychromatophilic RBCs (reticulocytes). (wikipedia.org)
  • A traditional application of methylene blue is the intravital or supravital staining of nerve fibers, an effect first described by Paul Ehrlich in 1887. (wikipedia.org)
  • The mast cell granules stain basophilic with Giemsa's stain and metachromatically with azure A or toluidine blue. (rupress.org)
  • This classification depends on whether granules can be distinguished in their cytoplasm using a light microscope and conventional staining methods). (leeds.ac.uk)
  • These cells have large acidophilic specific granules - these stain bright red, or reddish-purple. (leeds.ac.uk)
  • They are 14-16 µm in diameter, contain lots of deep blue staining granules (basic) and a bilobed nucleus.The granules contain heparin, histamine and serotonin. (leeds.ac.uk)
  • Not much cytoplasm can be seen, and it is basophilic (pale blue/purple staining). (leeds.ac.uk)
  • They have a small spherical nucleus and has abundant dark staining condensed chromatin. (leeds.ac.uk)
  • The amido liberated from acidophilic proteins carry positive charges and bind acid stain (Eosin) to become red. (baso.com.cn)
  • Cover the smear with 1~2 drops of Stain A for 15~20 seconds (the stain may dry at this time). (baso.com.cn)
  • Cover the smear with 2~3 drops of Stain B for 20~30 seconds. (baso.com.cn)
  • Neutrophil proteins, whose positive charges from the liberated amido are equivalent to the negative charges of liberated carboxyls, bind simultaneously both the acid stain and alkaline stain, and appear mauve. (baso.com.cn)
  • Proteins with different isoelectric points in spermatozoon and cells carry different charges under the same pH and thus they will selectively bind to different stains. (baso.com.cn)
  • Basophilic myeloblasts are produced from progenitor cells (CFU-Baso) under the influence of cytokines. (histologyguide.com)