Basic steps solid phase extraction. Medical search. Frequent questions

They were analyzed by means of a previously developed methodology, which involved solvent extraction, hydrophilic interaction liquid chromatography coupled to mass spectrometry, and a statistical analysis of the data (canonical discriminant analysis). (bvsalud.org)
Importance of the process: The Solvent Extraction(S.E.) Precipitation titration. (takebacknigeria.org)
This target would realize a dual purpose where the phosphate ore is considered as an alternative source of uraniu Solvent extraction is a common technique utilized for both industrial applications and in the laboratory. (takebacknigeria.org)
Process Of Extraction In Solvent Extraction Plant After the extraction process, the materials are fed onto a conveyor through a hopper. (spagades.com)
Liquid liquid extraction (LLE), also known as solvent extraction, has long been an effective method for clean-up lipophilic compounds in a complex aqueous matrix like e.g. biological fluids. (vertichrom.com)

Assay optimization and validation succeeded in reducing wash steps, centrifugation, thereby increasing sample throughput. (aurorabiomed.com)
Techniques based on DSPE permit minimization of additional steps such as precipitation, centrifugation, and filtration, which decreases the manipulation of the sample. (hindawi.com)
As well, most of these methods require repeated centrifugation steps, followed by removal of supernatants depending on the type of specimen and additional mechanical treatment. (excedr.com)
This adds multiple separation and purification steps to the overall process, such as repeated centrifugation. (excedr.com)
The screen protocols usually required one or more separation steps, for example centrifugation, phase extraction, filtration or precipitation, which were not conducive to operation at high levels of intensity. (ddw-online.com)

Dispersive solid phase extraction (DSPE) has been used as a pretreatment technique for the analysis of several compounds. (hindawi.com)
This technique has allowed the development of alternative methodologies such as dispersive solid phase extraction (DSPE), which is based on the addition of a sorbent directly into the analytical solution followed by dispersion favoring the contact between the sorbent and the analytes [ 14 , 15 ]. (hindawi.com)
This short review covers the use of ionic liquids (ILs) and polymeric ionic liquids (PILs) in solid-phase microextraction (SPME) and dispersive liquid-liquid microextraction (DLLME). (chromatographyonline.com)
ILs and PILs have been previously applied in various extraction methods including single-drop microextraction (SDME), liquid-phase microextraction (LPME), solid-phase microextraction (SPME), dispersive liquid-liquid microextraction (DLLME), hollow fiber-supported liquid membrane extraction (HFSLME), and solid-phase extraction (SPE) (2-7). (chromatographyonline.com)
The Association of Analytical Communities International (AOAC) QuEChERS method was used for a reduced 1.5-g amount of plant material and processed with a universal dispersive solid-phase extraction (dSPE) formulation. (spectroscopyonline.com)

Nucleic acid extraction (NAE) is the initial step for many molecular biology applications, so one of the first questions you have to answer when setting up a lab is what kind of RNA and DNA extraction method to use. (integra-biosciences.com)
What is nucleic acid extraction? (integra-biosciences.com)
The first nucleic acid extraction was performed in 1869 by the Swiss physician Friedrich Miescher , who isolated DNA from leukocytes when he was trying to determine the chemical composition of cells. (integra-biosciences.com)
But first, let's take a look back at two nucleic acid extraction methods that have been developed over the last 150 years, and show you why they should no longer be used, even though they are quick and easy to perform. (integra-biosciences.com)
Most labs now isolate nucleic acids by using sample- and application-specific kits for spin column or magnetic bead extraction workflows. (integra-biosciences.com)
Nucleic acid extraction and purification are basic yet key methods used in research across molecular biology, biopharmaceuticals, biotechnology, and various medical sciences. (excedr.com)
Nucleic acid sample preparation includes a wide range of extraction and purification techniques that are used to transform a sample from one that can't be directly analyzed into one that can, thus fitting the specific requirements of the analytical technique being performed downstream. (excedr.com)
Nucleic acid extraction and purification are the essential first steps for countless downstream applications, as high quality, highly pure DNA and RNA is important in a wide variety of research and clinical applications. (excedr.com)
In fact, the need to simplify has led to the development of multiple, semi-automated methods, such as solid-phase nucleic acid purification, which is available as an extraction kit. (excedr.com)

Using ultrasonic extraction in combination with a strong anion exchange solid phase extraction (SAE-SPE) technique, the filtration, preconcentration, and isolation of Cr.sup.VI in the presence of other chromium species and interferents was achieved. (cdc.gov)

Liquidliquid extraction. (takebacknigeria.org)
Liquidliquid extractions were preliminarily employed to generate six fractions, initially extracted by ethanol. (spagades.com)

SPE is in fact a method of chromatography, in the sense of having a mobile phase, carrying mixtures through a stationary phase, packed inside a column. (wikipedia.org)
The chemistry considerations for the selection of stationary phases and the mobile phases are very similar in SPE and in chromatography. (wikipedia.org)
Employing solid phase extraction sample preparation can also reduce harmful compounds introduced into the chromatography system thereby extending the longevity of the analytical column and instrument. (thermofisher.cn)
Solid phase extraction employs the same basic principles as chromatography. (thermofisher.cn)
Both sample preparations were coupled with reversed phase liquid chromatography and isotope dilution tandem mass spectrometry (LC-MS/MS) for analyte detection. (cdc.gov)
Based on the customers' sample requirements, the Oasis Method Development Tools will recommend the optimized solid-phase extraction (SPE) protocol to develop robust methods with high recovery for liquid chromatography and mass spectrometry applications. (technologynetworks.com)
The viability of quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was investigated using the Association of Analytical Communities International (AOAC) QuEChERS method with a hydration step modification (15). (spectroscopyonline.com)

These procedures are often followed by traditional protein extraction protocols (TCA/acetone precipitation or phenol extraction) to concentrate proteins and avoid protein degradation caused by abundant proteases. (frontiersin.org)

The influence of continuous ultrasonic extraction to the yield of dispersed protein was demonstrated by Moulton et al. (spagades.com)

Solid-phase extraction (SPE) is a solid-liquid extractive technique, by which compounds that are dissolved or suspended in a liquid mixture are separated, isolated or purified, from other compounds in this mixture, according to their physical and chemical properties. (wikipedia.org)
SPE uses the affinity of solutes, dissolved or suspended in a liquid (known as the mobile phase), to a solid packing inside a small column, through which the sample is passed (known as the stationary phase), to separate a mixture into desired and undesired components. (wikipedia.org)
The chromatographic process is harnessed to be an extractive technique, a solid-liquid extractive technique-taking advantage of large differences in the Keq, or equilibrium constant, of mixture components between the solid stationary phase and the mobile phase resulting, for a well-designed and executed separation. (wikipedia.org)
This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. (hindawi.com)
SPE uses significantly smaller volumes of solvent compared to liquid/liquid extraction (LLE) and supported liquid extraction (SLE) methods. (thermofisher.cn)
The coffee and tea examples are both of the liquid/solid type in which a compound (caffeine) is The distribution of a solute between two phases is an equilibrium condition. (takebacknigeria.org)
The three most common types of extractions are: liquid/liquid, liquid/solid , and acid/base (also known as a chemically active extraction). (takebacknigeria.org)
Extraction" means transference of compound(s) from a solid or liquid into a different solvent or phase, which is one of the methods used in organic chemistry laboratories to purify. (takebacknigeria.org)
Leaching is also known as solid-liquid extraction, lixiviation, washing etc. (takebacknigeria.org)
Liquid butane extraction. (takebacknigeria.org)
Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix.Common examples include liquid-liquid extraction, and solid phase extraction.The distribution of a solute between two phases is an equilibrium condition described by partition theory. (takebacknigeria.org)
The coffee and tea examples are both of the liquid/solid type in which a compound (caffeine) is isolated from a solid mixture by using a liquid extraction solvent (water). (takebacknigeria.org)
Supercritical fluid extraction utilizes a gas that has been pressurized and heated beyond its critical points so that it becomes a supercritical fluid (SCF), that is, a phase of matter where distinct liquid and gas phases do not exist (Fig. 1). (chemistryviews.org)
Once the SCF has penetrated the pores of the solid, it can selectively dissolve substances contained within the solid, similar to a liquid. (chemistryviews.org)
Changes in environmental conditions, such as temperature, pH, redox potential and organic ligand concentrations, can cause metals to be released from solid to liquid phase and sometimes cause contamination of surrounding waters in aquatic systems [ 3 ]. (intechopen.com)
Experiments were run at different operating temperatures and feed flow rates to investigate the effect on the dehydration products, which are mixtures of three phases: (1) a gas phase consisting of light hydrocarbons and carbon dioxide, (2) an organic liquid phase consisting of heavy hydrocarbons, and (3) an aqueous phase with dissolved oxygenated hydrocarbons. (auburn.edu)
Chemically pure 1-butene gas was liquefied in a pressure vessel where direct liquid-liquid extraction takes place and mole based distribution coefficient of 1.71 was attained for n-butanol single component extraction. (auburn.edu)
To compare the separation efficiency a two-step approach of adsorption on activated charcoal followed by liquid-solid extraction using 1-butene and percent recovery of each of the components, mole and mass based distribution coefficients were calculated and it was observed that other than for n-butanol, the distribution coefficients for other components increased compared to that of the direct liquid-liquid extraction process. (auburn.edu)
Clamp : Conical Percolators: Tincture Press: Hand Grinding Mill : Tablet Coating pan unit with blower : Disintegrator: Ball Mill : The removal of caffeine from coffee beans with dichloromethane is an example of a solid liquid extraction. (spagades.com)
Lab 3 - Extraction Objective Solid-liquid and liquid-liquid extractions are commonly performed by batch and continuous processes. (spagades.com)
Palm oil fractionation is a process to separate the palm oil into low melting point liquid phase and high melting point solid phase by controlling the cooling crystallization process. (edibleoilextractionmachine.com)
Liquid-liquid extractions with traditional separating funnel are often used. (vertichrom.com)
Aerosol - a suspension of solid and/or liquid particles in a gas (like air). (cdc.gov)

Extraction of hops with supercritical CO 2 was performed at temperatures between 40 and 50 C and pressures between 150 and 400 bar. (takebacknigeria.org)
Supercritical fluid extraction (SFE) also holds promise for the extraction of dichlorvos from soils (Lopez-Avila et al. (cdc.gov)
This year marks the 50th anniversary of the first patent describing the use of supercritical fluid extraction by Kurt Zosel, Max Planck Institute for Coal Research, Mülheim an der Ruhr, Germany. (chemistryviews.org)
It also marks the 40th anniversary of Zosel's US patent of the use of supercritical fluid extraction in the decaffeination of coffee. (chemistryviews.org)
The technique is most widely used, however, in the decaffeination of coffee through the extraction of caffeine from green coffee beans with supercritical CO 2 . (chemistryviews.org)

It will thoroughly discuss its metabolic pathways and physiology, disease-preventing and health-promoting properties, and further highlight the extraction procedures and detection methods in different matrices. (bvsalud.org)

Manual extraction requires multiple steps, increasing the risk of contamination. (excedr.com)

ILs and PILs exhibit a number of unique properties that make them highly useful extraction media in SPME and DLLME because of their high thermal stability, variable viscosity, and negligible vapor pressure. (chromatographyonline.com)
An overview of the basic principles of operation for both DLLME and SPME will be covered. (chromatographyonline.com)

Solid phase extraction can be used to isolate analytes of interest from a wide variety of matrices, including urine, blood, water, beverages, soil, and animal tissue. (wikipedia.org)
2 Due to a lack of purification steps, this method also results in low purity, and cannot efficiently remove PCR inhibitors from complex matrices. (integra-biosciences.com)
While the Maximum Selectivity Tool recommends ion-exchange and reversed-phase methods for aggressive sample cleanup in complex matrices. (technologynetworks.com)

The result is that either the desired analytes of interest or undesired impurities in the sample are retained on the stationary phase. (wikipedia.org)
The improved protocol was applied to recovery of analytes of interest from simulated water samples after solid phase extraction. (scielo.org.za)

The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. (wikipedia.org)
Ion exchange sorbents separate analytes based on electrostatic interactions between the analyte of interest and the positively or negatively charged groups on the stationary phase. (wikipedia.org)
1993). Analyte recovery was via extraction of the polymeric material with organic solvent, and determination typically by GC. (cdc.gov)
As a result, higher analyte enrichment factors and extraction efficiencies often can be achieved. (chromatographyonline.com)

1989), or through a clean-up of the matrix with no additional isolation steps (Unni et al. (cdc.gov)

For acetone, ethanol and butyric acid extraction separately from aqueous solvent the distribution coefficients were lower. (auburn.edu)

To extract a sufficient yield of high quality, purified RNA or DNA for your downstream applications, you have to find the best extraction method (or combination of methods) for your sample type. (integra-biosciences.com)
This study investigates a semicontinuous hot pressurized fluid extraction process and the scavenging activity on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical of the extract from Taiwan yams (Dioscorea alata). (spagades.com)
Continuous hot percolation process or soxhlet extraction or soxhleation 71 When active constituents of the drug are not freely soluble in the solvent or difficult to be displaced from the cells of the drug, then it becomes necessary to extract the crude drug by action of hot menstruum for a considerable length of time. (spagades.com)

If deposition of interfering matrix or enrichment of analytes is important, solid phase extraction, also called SPE, is often a necessary preparation step to be able to measure sensitively. (axelsemrau.de)
In this context, solid phase extraction plays an important role as a method for enrichment of the substances present in low concentrations. (mn-net.com)

A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. (biomedcentral.com)
multiple extractions required for high recovery rates, formation of emulsions, poor phase separation and high solvent consumption. (vertichrom.com)

Additionally, matrix effects were substantially decreased from previous reports for both extraction protocols. (cdc.gov)
Traditional extraction protocols and methods are often too difficult to use on a regular basis. (excedr.com)

And because extraction and purification are such an important part of biological research and clinical diagnostics, the methods used have needed to become simplified. (excedr.com)
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. (frontiersin.org)

RayCure Ceramic homogenizers are both compatible with RayCure QuEChERS extraction kits and clean-up kits, provide full solution for QuEChERS lab consumables. (raykolgroup.com)

The eluate was concentrated, and the phthalate metabolites chromatographically resolved by reversed-phase HPLC, detected by APCI- tandem mass spectrometry (APCI-MS/MS), and quantified by isotope dilution. (cdc.gov)
A fractionated elution of acidic and basic compounds can be applied in the analysis of pharmaceuticals and their metabolites. (vertichrom.com)

Serving the basic needs of a laboratory is at the core of Aurora, conventional tools preparing samples and dealing with the ever-increasing amount of casework submissions for determining concentrations of drugs of abuse is an aspect no lab can overlook. (aurorabiomed.com)
It is also considered highly complex, so not every laboratory technician is capable of performing extraction and purification manually. (excedr.com)
The resulting process is extremely time-intensive and requires the undivided attention of the laboratory technician at every step. (excedr.com)
Test compounds arrived in the laboratory as solids in bottles, the contents of which needed to be dissolved in an appropriate vehicle before being transferred to a reaction vessel, typically a 96-well microplate to which the appropriate biochemical reagents were added. (ddw-online.com)
The final step of connecting the vacuum pump to the laboratory device is about having the right connectors. (vacuubrand.com)
Blanks - samples containing extremely low concentrations of a contaminant , which are used to assess contamination from laboratory equipment and other steps in an analytical procedure. (cdc.gov)

The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. (wikipedia.org)
Electrolysis of molten compounds is Class 5: If K D is equal to 1, equivalent con-centrations exist in each phase. (takebacknigeria.org)
They have begun measuring how much of these drugs is present in rivers and streams as a first step toward understanding what effect the compounds might have on the environment. (acs.org)

As the sample passes through the stationary phase, the polar analytes in the sample will interact and retain on the polar sorbent while the solvent, and other non-polar impurities pass through the cartridge. (wikipedia.org)
The Micro Sample Volume Tool optimizes the right sorbent and protocol for sample volumes from 25 to 300 µL, eliminating the evaporation and reconstitution steps and concentrating users compound of interest by 15x. (technologynetworks.com)
With the exception of sorbent-based extraction procedures, many of these sample preparation methods involve the use of organic solvents. (chromatographyonline.com)

The portion that passes through the stationary phase is collected or discarded, depending on whether it contains the desired analytes or undesired impurities. (wikipedia.org)
If the portion retained on the stationary phase includes the desired analytes, they can then be removed from the stationary phase for collection in an additional step, in which the stationary phase is rinsed with an appropriate eluent. (wikipedia.org)
In the case of an incomplete extraction, the analytes do not have enough affinity for the stationary phase and part of them will remain in the permeate. (wikipedia.org)
A stationary phase of polar functionally bonded silicas with short carbons chains frequently makes up the solid phase. (wikipedia.org)
This stationary phase will adsorb polar molecules which can be collected with a more polar solvent. (wikipedia.org)
A stationary phase of silicon with carbon chains is commonly used. (wikipedia.org)
Like HPLC columns, there are many stationary phase options to choose from, such as reversed phase, ion exchange, normal phase, and mixed mode phases (see Table 1 and the SPE Solid Phase Selection page). (thermofisher.cn)
Continuous countercurrent extraction is efficiently performed using the high-speed coil planet centrifuge as shown in Figure 6.It enables extraction of a solute present in a large volume of the mobile phase into a small volume of the stationary phase retained in the coiled column. (spagades.com)

2 Sounds great but, unfortunately, this method lacks any purification steps, so often leads to inaccurate or unreliable results. (integra-biosciences.com)
These substances are not removed in the existing three purification steps of wastewater treatment plants (2) and re-enter the surface water and consequently the drinking water. (mn-net.com)

This study aimed to test a solution, based on integrating slow pyrolysis and chemical leaching, to separate phosphorus and other inorganics from char, obtaining an inorganic P-rich fertiliser and a C-rich solid usable for industrial purposes. (mdpi.com)

The service provider uses a truck-mounted hot water extraction machine to collect all water and residue. (spagades.com)
3. Add the first aliquot of organic extraction solvent and collect. (vertichrom.com)
Air filter - a solid matrix used in an air sampler to collect particulates from the air, which is drawn by an air pump through the filter. (cdc.gov)

This applies whether the solute is ^entering _ the droplet (droplet is the extraction solvent) or ^leaving _ it (the droplet is the raffinate phase). (spagades.com)

Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

The extraction procedure has been rated to be more efficient and effective than Continuous hot extraction. (spagades.com)

The course covers the basics, principles and limitations for the most common methods for multivariate statistics (analysis of variance, principal component analysis and partial least squares regression), experimental design (super-saturated and factorial designs) and method validation with a focus on practical use. (lu.se)

It is possible to have an incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. (wikipedia.org)

Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. (wikipedia.org)

VertiPak™ LLE has a broad range of applications for or the extraction of physiological fluids, such as urine, blood, serum, plasma, or others, in clinical chemistry. (vertichrom.com)

For extraction of ABE as a mixture, a preferential extraction of n-butanol was observed over the other components in the mixture due to its least polar characteristic among the components and thus higher solubility in the organic phase. (auburn.edu)
After 30 min solid potassium carbonate was added, the mixture was stirred for 1 hour, filtered and solvent removed to give 2-chloro-6-bromo-7,8-dihydro-5-quinolinone (2.86 g). (ambeed.com)

OAGB, as all Omega-loop surgical strategies, is characterized by the direct anastomosis of the biliopancreatic loop to the stomach, instead of interposing an alimentary loop as in the Roux-en-Y procedures. (nature.com)

An extraction buffer containing Tris-HCl, EDTA, sodium lauryl sarkosyl and water insoluble PVPP is added to the sample. (integra-biosciences.com)
For Chelex 100 extraction, an extraction buffer containing Chelex 100 resin, SDS, NP40 and Tween ® 20 is added to the sample. (integra-biosciences.com)
Preferably, the ultrasonication step is carried out in the presence of a slightly basic ammonium buffer and the complexing agent is 1,5-diphenylcarbazide. (cdc.gov)

Furthermore, conventional RNA and DNA extraction methods are not suitable for tough-to-lyse samples (e.g. yeast, human specimen, animal tissue or plant material), which require both chemical and physical cell lysis steps. (integra-biosciences.com)
Besides this relatively time-consuming step, even the modern GC-MS approaches still require at least 300 mg fresh plant material to reach dynamic range of a mass spectrometer [ 11 ]. (biomedcentral.com)
How to start an edible oil extraction plant? (edibleoilextractionmachine.com)
The process of setting up the edible oil extraction plant requires has some factors which have to be considered. (edibleoilextractionmachine.com)

Extraction Battery In the normal percolation process, the percolate is not of maximum concentration and as such very dilute. (takebacknigeria.org)

Method for the determination of hexavalent chromium using ultrasonication and strong anion exchange solid phase extraction. (cdc.gov)
Two types of automated solid phase extraction (SPE) were assessed for the determination of human exposure to fentanyls in urine. (cdc.gov)

Automation is a stepping stone in dealing with the pressures of sample turn around with what is considered to be a standard technique. (aurorabiomed.com)

Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. (thermofisher.cn)
Espresso - A Three-Step Preparation , Klaus Roth, ChemViews Magazine 2010 , May . (chemistryviews.org)
Knowing your and your team's exact sample preparation needs is an important step in deciding which method-whether it is manual or automated-as well as which instrument, is right for your lab. (excedr.com)
Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample preparations (SP3) on carboxylated or HILIC paramagnetic beads, and protein suspension trapping method (S-Trap) were evaluated for SDS removal and protein digestion from Arabidopsis thaliana (AT) lysate. (frontiersin.org)
More recently, several innovative attempts to get rid of contaminants were proposed, including the most widely adopted filter-aided sample preparation (FASP), the relatively new single-pot solid-phase-enhanced sample preparation (SP3), and the protein suspension trapping (S-Trap). (frontiersin.org)

As the name implies, this method consists of only one step. (integra-biosciences.com)
The method of extraction affects essential oil quality by way of pressure and temperatures applied. (takebacknigeria.org)
Merieux Nutrisciences' method is compliant with UNI EN 15662:2009. (foodnavigator.com)
Heat is then applied Soxhlet extraction is also known as the hot continuous e xtraction process the main advantage of this method is complete extraction in minimum amount of solvent. (spagades.com)
That is, myjoin is supposed to be an implementation of a string's join method cheating terms of basic string operations. (myselfie.co.za)

These can be useful for sample types that don't provide the desired output with spin column or magnetic bead extraction, e.g. samples with large particles such as soil or dust, or high volume samples such as chemostat cultures. (integra-biosciences.com)

It demonstrates the steps in focusing a compound light microscope from 10X to 100X. (cdc.gov)
This video provides step-by-step instructions on how to calibrate an ocular micrometer on a compound microscope with mathematical calculations. (cdc.gov)

Human urine samples were processed using enzymatic deconjugation of the glucuronides followed by solid-phase extraction. (cdc.gov)

SPE makes use of the same HPLC column solid phase packing materials in a single-use container such as plastic tubular cartridges (shown in Figure 1), 96-well plates, or pipette tips. (thermofisher.cn)

A typical solid phase extraction involves five basic steps. (wikipedia.org)

I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)

We measured vapor-phase nicotine and particulate matter 2.5 micrometers or less in diameter (PM2.5) as SHS markers at baseline (November-December 2010) and at follow-up (April-June 2011). (who.int)

SPE makes use of a solid phase material (there are many to choose from) that functions to retain the interfering substances, while solvents elute the sample, which is collected and analyzed. (thermofisher.cn)

Organisms2

Chemicals and Drugs4

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes3

Technology, Industry, Agriculture1

Health Care3

Solvent extraction5

Centrifugation5

Dispersive5

Nucleic9

Filtration1

Liquidliquid2

Chromatography7

Precipitation1

Ultrasonic extraction1

Liquid23

Supercritical5

Detection1

Increasing the risk of c1

SPME2

Matrices3

Analytes of interest2

Analyte4

Isolation1

Acetone1

Extract3

Enrichment2

Separation2

Protocols2

Biological2

QuEChERS1

Metabolites2

Laboratory6

Compounds3

Sorbent3

Stationary phase8

Purification steps2

Leaching1

Collect3

Solute1

Reconstitution steps1

Procedure1

Principles1

Elution1

Analytical1

Fluids1

Mixture2

Procedures1

Buffer3

Plant4

Percolation process1

Determination2

Automation1

Preparation5

Method5

Magnetic1

Compound2

Urine1

HPLC1

Involves1

Chemistry1

Nicotine1

Solvents1