ExtracellularAnti-LamininCollagenHeterotrimerBeta subunitAlpha3Engelbreth-Holm-SwarmAntibodiesAntibodyMouse lamininPlacental lamininMembranesIntegrinFibronectinImmunohistochemicalHeterotrimericIsoformsIsoformMoleculesChainsHuman placentaReactivityBasalLAMB3ReceptorSequenceOutgrowthSubstrateTumorIntegrinsHomologousHumansAlpha1GlycoproteinsMutationsIncluding keratinocytePrecursorMembrane zoneChain geneCell adhesionEpithelial CellsAlphaProtein encodedTumorsConsistsDifferentiationInvasionProteins
Extracellular16
- Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (wikipedia.org)
- Each laminin is a heterotrimer assembled from alpha, beta and gamma chain subunits, secreted and incorporated into cell-associated extracellular matrices. (embl.de)
- Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)
- Plectin is recruited into hemidesmosomes, multiprotein complexes that facilitate adhesion of epithelia to the basement membrane, thereby providing linkage between the intracellular keratin filaments to the laminins of the extracellular matrix. (embl.de)
- Plectin binds to hemidesmosomes through association of its actin-binding domain with the first pair of fibronectin type III repeats and a small part of the connecting segment of the integrin-beta4 subunit, the latter (integrin-alpha6,beta4) acting as a receptor for the extracellular matrix component laminin-5. (embl.de)
- This study aims to investigate the effects of oral administration of sodium nitrite on expression of laminin α5 in extracellular matrix of mice testicular interestitium using an Immunohistochemical (IHC) assessment. (biomedres.info)
- A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. (lookformedical.com)
- Although the mechanisms mediating the effects of the extracellular matrix (ECM) on breast carcinoma development in vivo are largely unknown, contributing factors may be adhesion mediated signaling and mechanical signals imparted on mammary epithelial cells from surrounding type-I collagen-rich stroma, either directly or across basement membrane proteins. (biomedcentral.com)
- An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
- HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
- Among the extracellular matrix compounds used in this study, two (collagen I and fibronectin) allowed only a weak attachment of cortical neurons to the substratum, while the others (collagen IV, laminin, and basal lamina from Engelbreth-Holm-Swarm sarcoma) allowed both firm attachment and moderate to extensive neurite outgrowth from neuronal cell bodies. (curehunter.com)
- Oncogenic RAS-mediated upregulation of matrix metalloproteinase 2 (MMP-2), MMP-9 and urokinase-type plasminogen activator (uPA) is critical for invasion through the basement membrane and extracellular matrix. (oncotarget.com)
- It is now well established that a key requirement for invasion and metastasis of cancer cells is the degradation of both the extracellular matrix (ECM) that surrounds the tumor and the basement membrane which presents the final barrier between the tumor cells and the blood stream [ 1 - 5 ]. (oncotarget.com)
- Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
- Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
- MECs synthesize key extracellular matrix (ECM) proteins of the meninges such as various collagens, as well as fibronectin, laminin and tenascin, important for pia basal lamina integrity and thus for neuronal support and function [ 7 ]. (biomedcentral.com)
Anti-Laminin2
- The IHC tests showed that the ECM of the mice testicular interstitial tissue showed reaction to anti-laminin-α5 antibody. (biomedres.info)
- The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
Collagen14
- Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
- Because type IV collagen and laminin are proteins found exclusively in the basement membrane, the antibodies to these two proteins selectively and specifically recognize basement membrane components. (biogenex.com)
- Immunohistochemical staining of laminin 332 and collagen IV along the floor of bullae can aid in the diagnosis, although the finding is nonspecific and can be seen in other bullous dermatoses where the schism lies above the lamina densa. (medscape.com)
- Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
- 3] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
- Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
- A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. (lookformedical.com)
- Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. (lookformedical.com)
- The alpha subunits of collagen type VI assemble into antiparallel, overlapping dimers which then align to form tetramers. (lookformedical.com)
- Several forms of collagen type V exist depending upon the composition of the subunits that form the trimer. (lookformedical.com)
- Collagen type XI is heterotrimer containing alpha1(XI), alpha2(XI) and alpha3(XI) subunits. (lookformedical.com)
- During liver fibrosis, the composition of the ECM changes from one dominated by collagen IV and laminin [ 4 ], to one of fibrillar collagen, predominantly collagen I and III, as well as fibronectin [ 5 ]. (biomedcentral.com)
- Immunohistochemical analysis of laminin and various types of collagen. (biopticka.cz)
- Distribution of type IV collagen and laminin. (biopticka.cz)
Heterotrimer4
- Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (wikipedia.org)
- Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. (wikipedia.org)
- Laminin is a heterotrimer made from α, β and γ chain subunits. (sigmaaldrich.com)
- The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer. (lookformedical.com)
Beta subunit1
Alpha32
- However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
- It is a heterotrimeric molecule composed of alpha1(V), alpha2(V) and alpha3(V) subunits. (lookformedical.com)
Engelbreth-Holm-Swarm1
- Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. (sigmaaldrich.com)
Antibodies9
- Antigenic specificity of antibodies from patients with linear basement membrane deposition of IgA. (medscape.com)
- Zenke Y, Nakano T, Eto H, Koga H, Hashimoto T. A case of vancomycin-associated linear IgA bullous dermatosis and IgA antibodies to the a3 subunit of laminin-332. (medscape.com)
- 4] These antibodies compromise the strength of the basement membrane zone, leading to skin fragility and trauma-induced blister formation. (medscape.com)
- alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
- Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
- Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
- The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
- Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
- In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
Antibody1
- In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
Mouse laminin3
- It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. (wikipedia.org)
- Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. (sigmaaldrich.com)
- LMA3_MOUSE Laminin alpha-3 chain precursor. (nig.ac.jp)
Placental laminin2
Membranes3
- Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
- Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
- It is a glycoprotein which is required for the structural scaffolding of basement membranes. (sigmaaldrich.com)
Integrin1
- To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
Fibronectin1
Immunohistochemical1
- Immunohistochemical staining of human stomach shows moderate membranous positivity in glandular cells, as well as staining in basement membrane. (bio-connect.nl)
Heterotrimeric2
- Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. (utsouthwestern.edu)
- Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
Isoforms1
- These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
Isoform1
- This gene encodes the gamma chain isoform laminin, gamma 1. (wikipedia.org)
Molecules2
- The protein encoded by this gene belongs to the laminin family of secreted molecules. (utsouthwestern.edu)
- Calculation of possible chain interactions of laminin chains with the B2t chain domain I/II indicated that the B2t chain can replace the B2 chain in some laminin molecules. (rupress.org)
Chains4
- Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (wikipedia.org)
- The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
- The amino acid sequence contained 21 amino acids of a putative signal peptide and 1,172 residues or alternatively 1,090 residues of a sequence with five distinct domains homologous to domains I-V in laminin chains. (rupress.org)
- Northern analysis showed that the B2t chain is expressed in several human fetal tissues but differently from the laminin B1 and B2 chains. (rupress.org)
Human placenta1
Reactivity1
- Three cases of linear IgA/IgG bullous dermatosis showing IgA and IgG reactivity with multiple antigens, particularly laminin-332. (medscape.com)
Basal3
- Hereditary junctional epidermolysis bullosa (JEB) is a recessive inherited blistering disorder of the skin and mucous membrane in which tissue separation occurs within the lamina lucida (i.e. under the basal plasma membrane of the basal keratinocytes and above the basement membrane) of the basement membrane zone (BMZ) at the dermal-epidermal junction [ 1 ]. (biomedcentral.com)
- They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
- The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
LAMB31
- LAMB3 / Laminin Beta 3 is a laminin that belongs to a family of basement membrane proteins. (lsbio.com)
Receptor1
- Also functions as a cell surface receptor for laminin. (joplink.net)
Sequence1
- Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)
Outgrowth1
- This combination increased outgrowth and branching relative to either laminin or hemolymph alone. (nature.com)
Substrate1
- We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. (nature.com)
Tumor1
- Degradation of the ECM facilitates invasion of tumor cells into the organ parenchyma, whereas degradation of the basement membrane is required for their entry into the blood stream as well as their metastasis to distant organs. (oncotarget.com)
Integrins1
Homologous2
- A truncated laminin chain homologous to the B2 chain: structure, spatial expression, and chromosomal assignment. (rupress.org)
- Comparison of the amino acid sequences showed that the novel laminin chain is homologous to the laminin B2 chain. (rupress.org)
Humans1
- Laminin subunit gamma-1 is a protein that in humans is encoded by the LAMC1 gene. (wikipedia.org)
Alpha11
- laminin alpha1,2 [Drosophila melanogas. (nig.ac.jp)
Glycoproteins1
- Laminins are glycoproteins that are involved in the organization of ECM. (biomedres.info)
Mutations1
- Epidermolysis bullosa is a family of bullous disorders caused by an absence of basement membrane components due to underlying gene mutations. (medscape.com)
Including keratinocyte1
- This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. (utsouthwestern.edu)
Precursor4
- Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. (joplink.net)
- laminin, alpha 1 precursor [Homo sa. (nig.ac.jp)
- LMA_DROME Laminin alpha chain precursor >gn. (nig.ac.jp)
- laminin, alpha 4 precursor [Homo sap. (nig.ac.jp)
Membrane zone1
- Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)
Chain gene1
- The gene for the laminin B2t chain (LAMB2T) was localized to chromosome 1q25-q31 in close proximity to the laminin B2 chain gene. (rupress.org)
Cell adhesion1
- Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. (joplink.net)
Epithelial Cells1
- By in situ hybridization expression of the B2t chain was localized to specific epithelial cells in skin, lung, and kidney as opposed to a general epithelial and endothelial cell expression of the laminin B2 chain in the same tissues. (rupress.org)
Alpha10
- laminin alpha chain (343.7 kD) (1L1. (nig.ac.jp)
- similar to laminin alpha-1 chain pr. (nig.ac.jp)
- laminin, alpha 1 [Mus musculus] >gnl. (nig.ac.jp)
- PREDICTED: similar to laminin alpha. (nig.ac.jp)
- alpha 3A chain of laminin-5 [Mus musc. (nig.ac.jp)
- laminin-5 alpha 3 chain [Rattus norveg. (nig.ac.jp)
- alpha 3B chain of laminin-5 [Homo sap. (nig.ac.jp)
- laminin alpha 3 splice variant a [Hom. (nig.ac.jp)
- laminin alpha 3 splice variant b2 [Ho. (nig.ac.jp)
- HSCs undergo phenotypic changes from quiescent vitamin A storing cells, involved in maintenance of the normal basement matrix, into proliferative myofibroblast-like cells, expressing myogenic markers such as alpha smooth muscle actin (αSMA). (biomedcentral.com)
Protein encoded1
- Each laminin chain is a multidomain protein encoded by a distinct gene. (wikipedia.org)
Tumors1
- In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. (biogenex.com)
Consists1
- Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
Differentiation1
- Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. (sigmaaldrich.com)
Invasion2
- A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. (elifesciences.org)
- A growing body of evidence suggests that cell cycle progression and invasion through a basement membrane are mutually exclusive cellular behaviors in both development and disease states (reviewed in Kohrman and Matus, 2017 ). (elifesciences.org)
Proteins1
- Skálová A, Leivo I. Basement membrane proteins in salivary gland tumours. (biopticka.cz)