• Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (wikipedia.org)
  • Each laminin is a heterotrimer assembled from alpha, beta and gamma chain subunits, secreted and incorporated into cell-associated extracellular matrices. (embl.de)
  • Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)
  • Plectin is recruited into hemidesmosomes, multiprotein complexes that facilitate adhesion of epithelia to the basement membrane, thereby providing linkage between the intracellular keratin filaments to the laminins of the extracellular matrix. (embl.de)
  • Plectin binds to hemidesmosomes through association of its actin-binding domain with the first pair of fibronectin type III repeats and a small part of the connecting segment of the integrin-beta4 subunit, the latter (integrin-alpha6,beta4) acting as a receptor for the extracellular matrix component laminin-5. (embl.de)
  • This study aims to investigate the effects of oral administration of sodium nitrite on expression of laminin α5 in extracellular matrix of mice testicular interestitium using an Immunohistochemical (IHC) assessment. (biomedres.info)
  • A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. (lookformedical.com)
  • Although the mechanisms mediating the effects of the extracellular matrix (ECM) on breast carcinoma development in vivo are largely unknown, contributing factors may be adhesion mediated signaling and mechanical signals imparted on mammary epithelial cells from surrounding type-I collagen-rich stroma, either directly or across basement membrane proteins. (biomedcentral.com)
  • An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
  • HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
  • Among the extracellular matrix compounds used in this study, two (collagen I and fibronectin) allowed only a weak attachment of cortical neurons to the substratum, while the others (collagen IV, laminin, and basal lamina from Engelbreth-Holm-Swarm sarcoma) allowed both firm attachment and moderate to extensive neurite outgrowth from neuronal cell bodies. (curehunter.com)
  • Oncogenic RAS-mediated upregulation of matrix metalloproteinase 2 (MMP-2), MMP-9 and urokinase-type plasminogen activator (uPA) is critical for invasion through the basement membrane and extracellular matrix. (oncotarget.com)
  • It is now well established that a key requirement for invasion and metastasis of cancer cells is the degradation of both the extracellular matrix (ECM) that surrounds the tumor and the basement membrane which presents the final barrier between the tumor cells and the blood stream [ 1 - 5 ]. (oncotarget.com)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • MECs synthesize key extracellular matrix (ECM) proteins of the meninges such as various collagens, as well as fibronectin, laminin and tenascin, important for pia basal lamina integrity and thus for neuronal support and function [ 7 ]. (biomedcentral.com)
  • The IHC tests showed that the ECM of the mice testicular interstitial tissue showed reaction to anti-laminin-α5 antibody. (biomedres.info)
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (wikipedia.org)
  • Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. (wikipedia.org)
  • Laminin is a heterotrimer made from α, β and γ chain subunits. (sigmaaldrich.com)
  • The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer. (lookformedical.com)
  • This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. (lsbio.com)
  • However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
  • It is a heterotrimeric molecule composed of alpha1(V), alpha2(V) and alpha3(V) subunits. (lookformedical.com)
  • Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. (sigmaaldrich.com)
  • Antigenic specificity of antibodies from patients with linear basement membrane deposition of IgA. (medscape.com)
  • Zenke Y, Nakano T, Eto H, Koga H, Hashimoto T. A case of vancomycin-associated linear IgA bullous dermatosis and IgA antibodies to the a3 subunit of laminin-332. (medscape.com)
  • 4] These antibodies compromise the strength of the basement membrane zone, leading to skin fragility and trauma-induced blister formation. (medscape.com)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
  • It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. (wikipedia.org)
  • Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. (sigmaaldrich.com)
  • LMA3_MOUSE Laminin alpha-3 chain precursor. (nig.ac.jp)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
  • Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
  • Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
  • It is a glycoprotein which is required for the structural scaffolding of basement membranes. (sigmaaldrich.com)
  • Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • Immunohistochemical staining of human stomach shows moderate membranous positivity in glandular cells, as well as staining in basement membrane. (bio-connect.nl)
  • Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. (utsouthwestern.edu)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • The protein encoded by this gene belongs to the laminin family of secreted molecules. (utsouthwestern.edu)
  • Calculation of possible chain interactions of laminin chains with the B2t chain domain I/II indicated that the B2t chain can replace the B2 chain in some laminin molecules. (rupress.org)
  • Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (wikipedia.org)
  • The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
  • The amino acid sequence contained 21 amino acids of a putative signal peptide and 1,172 residues or alternatively 1,090 residues of a sequence with five distinct domains homologous to domains I-V in laminin chains. (rupress.org)
  • Northern analysis showed that the B2t chain is expressed in several human fetal tissues but differently from the laminin B1 and B2 chains. (rupress.org)
  • Western blot analysis was used to partially characterize commercially available preparations of laminin isolated from the mouse EHS sarcoma and from human placenta. (nih.gov)
  • Three cases of linear IgA/IgG bullous dermatosis showing IgA and IgG reactivity with multiple antigens, particularly laminin-332. (medscape.com)
  • Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)
  • This combination increased outgrowth and branching relative to either laminin or hemolymph alone. (nature.com)
  • We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. (nature.com)
  • Degradation of the ECM facilitates invasion of tumor cells into the organ parenchyma, whereas degradation of the basement membrane is required for their entry into the blood stream as well as their metastasis to distant organs. (oncotarget.com)
  • The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)
  • A truncated laminin chain homologous to the B2 chain: structure, spatial expression, and chromosomal assignment. (rupress.org)
  • Comparison of the amino acid sequences showed that the novel laminin chain is homologous to the laminin B2 chain. (rupress.org)
  • Laminin subunit gamma-1 is a protein that in humans is encoded by the LAMC1 gene. (wikipedia.org)
  • laminin alpha1,2 [Drosophila melanogas. (nig.ac.jp)
  • Epidermolysis bullosa is a family of bullous disorders caused by an absence of basement membrane components due to underlying gene mutations. (medscape.com)
  • This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. (utsouthwestern.edu)
  • Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. (joplink.net)
  • laminin, alpha 1 precursor [Homo sa. (nig.ac.jp)
  • LMA_DROME Laminin alpha chain precursor >gn. (nig.ac.jp)
  • laminin, alpha 4 precursor [Homo sap. (nig.ac.jp)
  • The gene for the laminin B2t chain (LAMB2T) was localized to chromosome 1q25-q31 in close proximity to the laminin B2 chain gene. (rupress.org)
  • laminin alpha chain (343.7 kD) (1L1. (nig.ac.jp)
  • similar to laminin alpha-1 chain pr. (nig.ac.jp)
  • laminin, alpha 1 [Mus musculus] >gnl. (nig.ac.jp)
  • PREDICTED: similar to laminin alpha. (nig.ac.jp)
  • alpha 3A chain of laminin-5 [Mus musc. (nig.ac.jp)
  • laminin-5 alpha 3 chain [Rattus norveg. (nig.ac.jp)
  • alpha 3B chain of laminin-5 [Homo sap. (nig.ac.jp)
  • laminin alpha 3 splice variant a [Hom. (nig.ac.jp)
  • laminin alpha 3 splice variant b2 [Ho. (nig.ac.jp)
  • HSCs undergo phenotypic changes from quiescent vitamin A storing cells, involved in maintenance of the normal basement matrix, into proliferative myofibroblast-like cells, expressing myogenic markers such as alpha smooth muscle actin (αSMA). (biomedcentral.com)
  • In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. (biogenex.com)
  • Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
  • Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. (sigmaaldrich.com)