Basement signals receptors laminin. Medical search. Frequent questions

No specific or efficient treatment exists for Alport syndrome, an X-linked hereditary disease caused by mutations in collagen type IV, a crucial component of the glomerular basement membrane. (hindawi.com)
Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (unifi.it)
Binding sites for laminin and collagen IV are present on the C-terminal globule but not yet precisely localized. (unifi.it)
BMs are specialized structures that underlie epithelia and surround organs and mainly comprise collagen IV, laminins, and heparan sulfate proteoglycans (HSPG). (nature.com)
The AM basement membrane contains laminin, fibronectin, and collagen type IV and VII, similar to the basement membrane of conjunctival tissue, but more than corneal epithelium. (touchophthalmology.com)
During liver fibrosis, the composition of the ECM changes from one dominated by collagen IV and laminin [ 4 ], to one of fibrillar collagen, predominantly collagen I and III, as well as fibronectin [ 5 ]. (biomedcentral.com)
HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
The collagen and laminin of basement mb, and the adhesive glycoprotein of the ECM like fibronectin and laminin hold the cells via integrins in the cell mb. (medicinembbs.org)
Migration and proliferation of parenchymal and connective tissue cellsBasement membrane crucial to wound healing: collagen in the bm binds to cells via proteins like integrins, fibronectin and laminin. (medicinembbs.org)
Type IV collagen is the main component of the basement membrane that gives strength to the blood-gas barrier (BGB). (biomedcentral.com)
Our study showed that type IV collagen and, therefore the basement membrane, play fundamental roles in coordinating alveolar morphogenesis. (biomedcentral.com)
Accordingly, to clarify the molecular basis of tissue-specific differentiation and disease, a variety of 3D systems exploiting natural ECMs have been developed, such as reconstituted basement membrane (rBM) and purified collagen hydrogels. (ucsf.edu)
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. (cusabio.com)

These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. (wikipedia.org)
We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. (nature.com)
Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)
Laminins are extracellular matrix proteins that reside in the basement membrane and provide structural support in addition to promoting cellular adhesion and migration. (bvsalud.org)
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. (cancer-genetics.org)
The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. (embl.de)
Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. (embl.de)
8,9 The underlying stromal matrix contains proteins that suppress transforming growth factor (TGF)-β signaling in fibroblasts, and consequently inhibit the proliferation and differentiation into myofibroblasts, extracellular matrix production, and scar formation. (touchophthalmology.com)

We suggest the potential roles certain integrins, the urokinase plasminogen activator (uPA), its receptor (uPAR), and plasmin may play in the pathogenesis of Alport kidney disease. (hindawi.com)
This could be considered to be part of a feedback loop as changes in ECM composition results in changes in HSC cell surface receptors, particularly integrins indicating that the ECM should not be regarded simply as an inert physical scaffold but a dynamic structure that is able to influence the phenotype of HSCs [ 2 , 8 , 9 ]. (biomedcentral.com)
Given the multi-domain architecture of Ln5, it seems conceivable that other receptors in addition to integrins interact with one of its many potential ligand sites to mediate its diverse cellular functions, including activation of EGFR signaling. (spandidos-publications.com)
Their data suggest that the ALL cells co-opt a pathway that involves contact between integrins expressed on the ALL cells and the basement membrane of these vessels. (medicilon.com)
The extracellular matrix (ECM), the noncellular component of the microenvironment, influences cell growth, survival, migration and tissue-specific differentiation through a repertoire of receptors including integrins, syndecans and discoidin receptors. (ucsf.edu)

Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. (joplink.net)
This review focuses on the research to date detailing the expression of laminin isoforms, their potential function, as well as known pathways involved in laminin signaling in metabolic tissues. (bvsalud.org)
PKC family of serine-threonine kinases plays key roles in many of the signaling pathways that control cellular function, including those mediated through integrin signaling. (mirnamimic.com)
Endostatin has been implicated in several signaling pathways, such as downregulation of c-myc (Shichiri and Hirata (2001) Faseb J, 15: 1044-1053), cyclin-D1 (Hanai et al. (justia.com)
The researchers' work focused on PI3K, a key regulator of signaling pathways needed for growth, survival, and invasion by cancer cells. (medicilon.com)

In metabolic tissues such as the pancreas, adipose, muscle, and liver, laminin isoforms are expressed in discrete temporal and spatial patterns suggesting that certain isoforms may support the development and function of particular metabolic cell types. (bvsalud.org)
We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (utsouthwestern.edu)

Interaction with ECM is a major driver of HSC morphology [ 11 ], and signal transduction from integrin activation occurs at least partly through aggregation and subsequent activation of focal adhesion kinases (FAKs) by autophosphorylation of tyrosine 397 (Y397). (biomedcentral.com)

They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. (wikipedia.org)
Through interactions with cell surface receptors, laminins stimulate intracellular signaling cascades which direct specific survival and differentiation outcomes. (bvsalud.org)
Conversely, atRA or TTNPB promoted β-cell differentiation accompanied by enhanced expression of vascular basement component, laminin. (bath.ac.uk)
2-5 The basement membrane provides a substrate that promotes epithelial cell adhesion, migration, and differentiation 5-7 and prevents epithelial cell apoptosis. (touchophthalmology.com)
In three-dimensional reconstituted basement membrane culture, they undergo a well-defined program of proliferation, differentiation, and growth arrest, forming acinar structures that recapitulate many aspects of mammary architecture in vivo . (biomedcentral.com)
Here we report on the growth and differentiation properties of these three matched cell lines in three-dimensional basement membrane culture. (biomedcentral.com)

The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)

RPSA was initially identified as a laminin binding protein. (wikipedia.org)
Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)

That the sensing of stromal rigidity originates at the adhesion nexus, the junctional structure that links cells to the ECM via integrin receptors. (manchester.ac.uk)
We are establishing new techniques for global analyses of the adhesion nexus in three-dimensional (3D) pancreatic organoids and defining how variations in extracellular rigidity initiate signalling at this site to influence cell cycle progression. (manchester.ac.uk)
In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
Go¨6976 also induced laminin-dependent cell adhesion in NIH/3T3 and CV-1 fibroblasts, suggesting of a mechanism that may be common to multiple cell-types. (mirnamimic.com)
A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)
Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)
Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
Our results demonstrate that cytoglobin expression is correlated with a more quiescent phenotype of stellate cells in culture and that cytoglobin is regulated by the extracellular matrix through integrin signalling dependent on activation of focal adhesion kinase. (biomedcentral.com)
Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
Universal features of breast cancer include a loss of control of cell proliferation and organization, a loss of cell polarity, as well as a loss of cell: cell adhesion and cell: basement membrane adhesion. (biomedcentral.com)
The second part of our research is directed towards characterizing the role of and molecular mechanisms whereby mechanical force regulates human embryonic stem cell fate using 2D/3D natural and synthetic matrices and a force reactor with a focus on adhesion signaling and epigenetics. (ucsf.edu)

Laminin subunit beta 1 (LAMB1) is involved in attachment, migration, and organization during development, and its elevated expression has been associated with several cancers. (bvsalud.org)

Glioblastoma multiforme (GBM) is a highly aggressive and heterogeneous form of primary brain tumors, driven by a complex repertoire of oncogenic alterations, including the constitutively active epidermal growth factor receptor (EGFRvIII). (cancer-genetics.org)
Tyrosine kinase inhibitors (TKIs) targeting epidermal growth factor receptor (EGFR), including gefitinib and erlotinib, have become the standard first-line therapy for patients with advanced non-small cell lung cancers (NSCLCs) harboring activating EGFR mutations ( 4 , 5 ). (spandidos-publications.com)

Weak sequence homologies (25%) were detected between some regions of nidogen, the LDL receptor, thyroglobulin and the EGF precursor. (unifi.it)

Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)

Notably, the addition of hemolymph to laminin substrates accelerated growth cone migration rate over ten-fold. (nature.com)
Laminin 5 (Ln5) is an extracellular matrix protein that plays an important role in cell migration and tumor invasion. (spandidos-publications.com)
The α6 integrin binds laminin, a component of the extracellular matrix that surrounds large blood vessels, the meninges, and the CNS choroid plexus to help facilitate cell migration. (medicilon.com)
They found that α6 integrin-laminin interactions mediated the migration of ALL cells toward the cerebrospinal fluid in vitro . (medicilon.com)
An understanding of how biomechanical and biochemical signals influence directional migration to these sites of tissue escape could help develop therapies to slow or halt metastasis. (ucsf.edu)
The basement membrane is also important for epithelial migration, and abnormalities in basement membrane structure, whether due to trauma (eg, recurrent erosion syndrome) or dystrophy (eg, basement membrane dystrophy), can lead to persistence of corneal epithelial defects and stromal ulceration. (medscape.com)

Both RPSA and laminin belong to the heparin/heparan sulfate interactome. (wikipedia.org)

Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)

The uPA converts cell surface-bound plasminogen to plasmin, a process that is regulated by the binding of plasminogen to specific receptors on the cell surface, however, the identity of the plasminogen receptors that function in this capacity is unclear. (oncotarget.com)

We conclude that the Wnt/beta-catenin signaling pathway acting downstream of FGF signaling can serve as a negative regulatory mechanism in the process of SMG branching morphogenesis through Lama5. (bvsalud.org)
There are numerous downstream signalling targets of FAK with many of the effects ultimately mediated by changes in gene expression [ 11 - 13 ]. (biomedcentral.com)

This combination increased outgrowth and branching relative to either laminin or hemolymph alone. (nature.com)

Many of the effects of laminin are mediated through interactions with cell surface receptors. (wikipedia.org)
The roles and possible interactions of the Wnt/beta-catenin signaling pathway, FGF signaling, and lama5 in the branching process were investigated by morphogenesis assays and gene expression patterns. (bvsalud.org)

Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)

The RPSA gene encodes a multifunctional protein, which is both a ribosomal protein and a high-affinity, non-integrin laminin receptor. (wikipedia.org)
This gene encodes the beta chain isoform laminin, beta 1. (utsouthwestern.edu)

We will also discuss the current biomedical therapies involving laminins in these tissues in addition to prospective applications, with the goal being to encourage future investigation of laminins in the context of metabolic disease. (bvsalud.org)
Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. (utsouthwestern.edu)
As a basement membrane protein, COL4A1 is commonly expressed in many tissues and cell types. (cusabio.com)

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (wikipedia.org)

Laminins are multifunctional basement membrane glycoproteins composed of a, b, and g polypeptide chains. (mirnamimic.com)
Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (utsouthwestern.edu)

β-Cells depend on the islet basement membrane (BM). (nature.com)
Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
The findings explain why maternal vitamin A deficiency affects fetal islet development and support an essential role of retinoid signaling in regulating vascularized islet development. (bath.ac.uk)

Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)

Finally, we show that Wnt/beta-catenin signaling regulates branching morphogenesis through Lama5. (bvsalud.org)
The tumor-suppressor gene PTEN negatively regulates the PI3K/Akt signaling pathway ( 13 ). (spandidos-publications.com)

Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)

2002) J Biol Chem, 277: 27872-27879), and inhibition of the wnt-signaling pathway (Hanai et al. (justia.com)

U0126, an extracellular signal-regulated kinase (ERK) inhibitor, regulated the expression of LAMB1 in gastric cancer cells. (bvsalud.org)
This study demonstrated that the Receptor for Activated C-Kinase 1 (RACK1) is important in SC function. (sdbonline.org)

Features of tissue morphogenesis were assessed, including proliferation, basement membrane formation, polarization of alpha-6 beta-4 integrin to the basement membrane, formation of cell:cell junctions, and apoptosis for luminal clearance. (biomedcentral.com)

The anti-inflammatory activity of AM is attributed to numerous mediators, including interleukin (IL)-1 receptor antagonist, IL-10, activin, inhibin, and inter-α-trypsin inhibitor. (touchophthalmology.com)

A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. (utsouthwestern.edu)
It is now well established that a key requirement for invasion and metastasis of cancer cells is the degradation of both the extracellular matrix (ECM) that surrounds the tumor and the basement membrane which presents the final barrier between the tumor cells and the blood stream [ 1 - 5 ]. (oncotarget.com)
Degradation of the ECM facilitates invasion of tumor cells into the organ parenchyma, whereas degradation of the basement membrane is required for their entry into the blood stream as well as their metastasis to distant organs. (oncotarget.com)

an inhibitor of retinaldehyde dehydrogenase), all-trans retinoic acid (atRA) or retinoic acid receptor agonist (E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthylenyl)-1-propenyl] benzoic acid (TTNPB) was carried out. (bath.ac.uk)

Oncogenic RAS-mediated upregulation of matrix metalloproteinase 2 (MMP-2), MMP-9 and urokinase-type plasminogen activator (uPA) is critical for invasion through the basement membrane and extracellular matrix. (oncotarget.com)

2003) J Biol Chem, 278: 37895-37901), blockage of VEGF signaling (Hajitou et al. (justia.com)

One part of our research program focuses on clarifying how mechanical force (ECM stiffness and cell contractility) modulate breast tumor progression and treatment efficacy with a focus on signaling and epigenetics using two and three dimensional (2D/3D) monolayer and organotypic culture models, xenograft/syngeneic and transgenic mouse models and clinical specimens and a repertoire of approaches to measure and manipulate mechanical force. (ucsf.edu)

Each laminin chain is a multidomain protein encoded by a distinct gene. (utsouthwestern.edu)

The main function of metalloproteinases is to degrade extracellular matrix and basement membrane components. (medscape.com)

In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)

RPSA is also a receptor for small molecules. (wikipedia.org)

Both recombinant N-domain and C-domain of RPSA bind laminin in vitro, and they bind with similar dissociation constants (300 nM). (wikipedia.org)

In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)

In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)

[ 1 , 2 ] Although the chief target of the autoimmune attack in most cases is the skeletal muscle nicotinic acetylcholine receptor (nAChR), other antigenic targets that are components of the neuromuscular junction (NMJ) have also been implicated. (medscape.com)

Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment. (hindawi.com)

RPSA is a ribosomal protein which has acquired the function of laminin receptor during evolution. (wikipedia.org)

It also acts as a cell surface receptor, in particular for laminin, and is involved in several pathogenic processes. (wikipedia.org)

My group is exploring the molecular mechanisms whereby ECM receptors modulate cell fate and tumorigenesis. (ucsf.edu)

Further experiments indicated that FGF signaling most likely acts upstream as a negative regulator of the canonical Wnt pathway during the branching process, whose effect could be partially reversed by Wnt3a. (bvsalud.org)

Hereditary junctional epidermolysis bullosa (JEB) is a recessive inherited blistering disorder of the skin and mucous membrane in which tissue separation occurs within the lamina lucida (i.e. under the basal plasma membrane of the basal keratinocytes and above the basement membrane) of the basement membrane zone (BMZ) at the dermal-epidermal junction [ 1 ]. (biomedcentral.com)

Anatomy15

Organisms2

Diseases3

Chemicals and Drugs35

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes18

Disciplines and Occupations1

Information Science4

Collagen14

Proteins8

Integrins5

Pathways5

Isoforms3

Transduction1

Differentiation6

Fibronectin and laminin1

Binding protein2

Adhesion11

Subunit1

Epidermal growth f2

Precursor1

Heterotrimeric1

Migration6

Heparan sulfate1

Intracellular1

Specific receptors1

Downstream2

Outgrowth1

Interactions2

Vascular2

Gene encodes2

Tissues3

Membranes1

Chains2

Islet3

Polypeptides1

Regulates2

Fibroblasts1

Inhibition1

Kinase2

Morphogenesis1

Interleukin1

Metastasis3

Inhibitor1

Plasminogen activator1

VEGF1

Tumor1

Multidomain1

Metalloproteinases1

Chain1

Molecules1

Recombinant1

Antibodies1

Assays1

Antigenic1

Alterations1

Ribosomal1

Processes1

Cell fate1

Regulator1

Tissue1