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Basement MembraneLamininReceptors, LamininHeterotrimeric GTP-Binding ProteinsGlomerular Basement MembraneAnti-Glomerular Basement Membrane DiseaseKidney GlomerulusCell MembraneCollagen Type IVCollagenMembranesGTP-Binding Protein alpha SubunitsMembrane LipidsMicroscopy, ElectronIntracellular MembranesExtracellular MatrixMembrane PotentialsMembranes, ArtificialHeparan Sulfate ProteoglycansGTP-Binding ProteinsGTP-Binding Protein beta SubunitsFluorescent Antibody TechniqueMolecular Sequence DataCell AdhesionFibronectinsAmino Acid SequenceGTP-Binding Protein gamma SubunitsGTP-Binding Protein alpha Subunits, Gi-GoProtein BindingSignal TransductionCells, CulturedNephritis, HereditaryGlomerulonephritisMembrane GlycoproteinsHeparitin SulfateCell LineErythrocyte MembraneCell Membrane PermeabilityMembrane FluidityEpitheliumProteoglycansDystroglycansIntegrinsGTP-Binding Protein alpha Subunits, G12-G13ImmunohistochemistryRGS ProteinsGTP-Binding Protein alpha Subunit, Gi2KidneyCapillariesProtein Structure, TertiaryCell MovementEpithelial CellsGTP-Binding Protein alpha Subunits, Gq-G11MutationModels, BiologicalMicroscopy, FluorescenceElectrophoresis, Polyacrylamide GelDescemet MembraneCattleIntegrin alpha6beta1AntibodiesRecombinant ProteinsProtein TransportMolecular WeightGTP-Binding Protein alpha Subunits, GsExtracellular Matrix ProteinsBase SequenceBinding SitesChondroitin Sulfate ProteoglycansIntegrin alpha3beta1Guanosine 5'-O-(3-Thiotriphosphate)Integrin alpha6Blotting, WesternRNA, MessengerAluminum CompoundsCell Adhesion MoleculesHemidesmosomesMicroscopy, ImmunoelectronRecombinant Fusion ProteinsMembrane Transport ProteinsMacromolecular SubstancesProteinuriaRabbitsFluorescent Antibody Technique, IndirectGlycoproteinsTime FactorsSequence Homology, Amino AcidPeptide FragmentsCarrier ProteinsKidney TubulesCollagen Type XVIIIAntigens, CD29TransfectionProtein SubunitsProtein ConformationNephritisMicroscopy, Electron, TransmissionCalciumModels, MolecularAntibodies, Monoclonal
ProteinsSubunitGlycoproteinsProteinStructuralIntracellularTissuesEmbryonicCollagenAlpha3AttachmentVitroTissueBullousFunctionAlphaCellsFoundZoneHumanGlycoproteinFibronectin6q22-q23ChainsSubunitsSkeletal muscleBasalMDC1ADeficientMoleculesIntegrinMatrixDeficiencyMutationsLungPresenceDevelopmentComplexEnvironment
Proteins8
  • Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. (wikipedia.org)
  • The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. (embl.de)
  • Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. (embl.de)
  • To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay. (biomedcentral.com)
  • Laminins are extracellular matrix proteins that reside in the basement membrane and provide structural support in addition to promoting cellular adhesion and migration. (bvsalud.org)
  • GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. (nature.com)
  • Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
  • He is known for his work on laminins - huge proteins that are a key component of all basement membranes. (imperial.ac.uk)
Subunit2
  • Laminin subunit alpha-5 is a protein that in humans is encoded by the LAMA5 gene. (wikipedia.org)
  • Laminin subunit beta 1 (LAMB1) is involved in attachment, migration, and organization during development, and its elevated expression has been associated with several cancers. (bvsalud.org)
Glycoproteins1
  • In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. (wikipedia.org)
Protein7
  • The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. (wikipedia.org)
  • Using a new method for construction and database searches of sequence consensus strings, we have identified a new superfamily of protein modules comprising laminin G, thrombospondin N and the pentraxin families. (embl.de)
  • Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC. (biomedcentral.com)
  • Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. (nature.com)
  • As a basement membrane protein, COL4A1 is commonly expressed in many tissues and cell types. (cusabio.com)
Structural3
Intracellular2
  • Through interactions with cell surface receptors, laminins stimulate intracellular signaling cascades which direct specific survival and differentiation outcomes. (bvsalud.org)
  • Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
Tissues5
  • Components of the extracellular matrix exert myriad effects on tissues throughout the body. (wikipedia.org)
  • It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. (wikipedia.org)
  • In metabolic tissues such as the pancreas, adipose, muscle, and liver, laminin isoforms are expressed in discrete temporal and spatial patterns suggesting that certain isoforms may support the development and function of particular metabolic cell types. (bvsalud.org)
  • This review focuses on the research to date detailing the expression of laminin isoforms, their potential function, as well as known pathways involved in laminin signaling in metabolic tissues. (bvsalud.org)
  • We will also discuss the current biomedical therapies involving laminins in these tissues in addition to prospective applications, with the goal being to encourage future investigation of laminins in the context of metabolic disease. (bvsalud.org)
Embryonic2
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
Collagen6
Alpha31
  • It is a heterotrimeric molecule composed of alpha1(V), alpha2(V) and alpha3(V) subunits. (lookformedical.com)
Attachment1
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
Vitro1
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
Tissue1
  • Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC were analyzed using immunohistochemistry on tissue microarray containing 205 ESCC and 173 adjacent precursor lesions as well as corresponding normal mucosa. (biomedcentral.com)
Bullous2
  • Epidermolysis bullosa is a family of bullous disorders caused by an absence of basement membrane components due to underlying gene mutations. (medscape.com)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
Function1
  • Laminin G domains can vary in their function, and a variety of binding functions have been ascribed to different LamG modules. (embl.de)
Alpha1
  • Laminin, alpha 5 has been shown to interact with BCAM. (wikipedia.org)
Cells3
  • U0126, an extracellular signal-regulated kinase (ERK) inhibitor, regulated the expression of LAMB1 in gastric cancer cells. (bvsalud.org)
  • However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
  • Immune disturbance as well as destruction of basal epidermal cells and base membrane by GVHD may result in the induction of autoimmune blistering diseases with unusual clinical and laboratory manifestations. (morulaivf.com)
Found1
Zone1
  • Direct immunofluorescence revealed a linear deposition of IgG on the base membrane zone. (morulaivf.com)
Human1
Glycoprotein2
  • Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. (nih.gov)
  • Laminin-332 (LM-332, formerly termed laminin-5) is a heterotrimeric glycoprotein that regulates cell adhesion and migration. (nih.gov)
Fibronectin3
  • Secreted molecules also contribute to the extracellular matrix (fibronectin, laminin, reelin). (frontiersin.org)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
6q22-q231
  • MDC1A is caused by mutations in the laminin α2 gene ( LAMA2 ) linked to chromosome 6q22-q23 and inherited as autosomal recessive. (medscape.com)
Chains3
  • Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (nih.gov)
  • Each laminin is a heterodimer composed of a heavy chain (α) and two light chains (β and γ). (medscape.com)
  • Extra-cellular secretion of laminin β3 and γ2 chains was found in 2 of the 10 cell lines. (nih.gov)
Subunits1
Skeletal muscle1
  • The major laminin of adult skeletal muscle is laminin-2 (also known as merosin), and only mutations of LAMA2 gene encoding laminin α2 cause muscular dystrophy. (medscape.com)
Basal3
  • At the superior aspect of the BMZ, keratin-containing intermediate filaments of the basal cell cytoskeleton insert on basal cell plasma membrane condensations termed hemidesmosomes. (medscape.com)
  • Anchoring filaments extend from the basal cell plasma membrane into the extracellular environment and span the lamina lucida, connecting hemidesmosomes with the lamina densa. (medscape.com)
  • Immune disturbance as well as destruction of basal epidermal cells and base membrane by GVHD may result in the induction of autoimmune blistering diseases with unusual clinical and laboratory manifestations. (morulaivf.com)
MDC1A1
  • Clinical variants of MDC1A occur with some mutations when only partial laminin-α2 deficiency is present. (medscape.com)
Deficient3
  • Similarly, the severity of fibrosis induced by an adenovirus encoding an active form of transforming growth factor-β was worse in mice deficient in α3 laminin in the lung. (nih.gov)
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
  • In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
Molecules1
  • In addition, anchoring filaments contain the molecules laminin-332 and laminin-6. (medscape.com)
Integrin1
  • These structures contain the extracellular portions of collagen XVII (BP180) and alpha-6-beta-4 integrin. (medscape.com)
Matrix2
Deficiency1
  • Clues to the presence of laminin-α2 deficiency include MRI abnormalities, seizures, and demyelinating neuropathy. (medscape.com)
Mutations1
  • Epidermolysis bullosa is a family of bullous disorders caused by an absence of basement membrane components due to underlying gene mutations. (medscape.com)
Lung1
  • Lung-specific loss of α3 laminin worsens bleomycin-induced pulmonary fibrosis. (nih.gov)
Presence1
  • White-matter changes are not correlated with the amount of laminin-α2, the patient's intelligence, or the presence of seizures. (medscape.com)
Development2
  • This review will focus on extracellular factors which influence RG morphology and pyramidal neuronal migration during normal development and their perturbations in pathology. (frontiersin.org)
  • Changes in one or several of these components influencing the RG extracellular environment can disrupt the development or maintenance of RG architecture on which neuronal migration relies, leading to a range of cortical malformations. (frontiersin.org)
Complex1
Environment1
  • Fewer studies have identified the influence of the extracellular environment on this architecture. (frontiersin.org)