• According to subsequent experiments, we found that the YIGSR peptide strongly enhanced collagen type 1 synthesis without changing cell proliferation or cellular MMP-1 level. (peptide.com)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • 4,19-21 The immunosuppressive ability of AM to inhibit a mixed lymphocyte reaction appears to be partly mediated by a soluble factor, resulting in decreased alloreactive T-cell proliferation and Th1 and Th2 cytokine production. (touchophthalmology.com)
  • Deposition of collagen and proliferation of smooth muscle cells are the response to chronic inflammation and injury of the submucosa leading to fibrosis and thickening of the submucosa and the smooth muscle layers of the bowel wall. (bmj.com)
  • Loss of GPR56 does not affect cell proliferation, migration, or neurite outgrowth. (jneurosci.org)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • BioCoat products support cell attachment, proliferation and growth, as well as differentiation of primary cells and cell lines in serum-containing and serum-free cultures. (szabo-scandic.com)
  • Since all cells involved in the destruction of islets in T1D come into contact with ECM, we postulate that these components of the ECM may, in part, regulate key events in pathogenesis of T1D such as β-cell death, immune cell migration, proliferation and invasion, cytokine expression and release, and antigen presentation. (npod.org)
  • Osathanon T, Bespinyowong K, Arksornnukit M, Takahashi H, Pavasant P. "Human osteoblast-like cell spreading and proliferation on Ti-6Al-7Nb surfaces of varying roughness. (sciepub.com)
  • Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
  • A sufficient supply of progenitor stem cells to facilitate epithelial cell proliferation is important for the cornea. (medscape.com)
  • This matrix lends structural as well as biochemical support to the cells surrounded by it, and forms a foundation for their growth and proliferation. (biologywise.com)
  • It consists of a variety of growth and differentiating factors that regulate and influence development, migration, proliferation, shape, and metabolic functions of the cells. (biologywise.com)
  • The effect of varying concentrations of the AQP1 inhibitor AqB013 was tested on human colon cancer cell lines expressing high versus low levels of AQP1, using wound closure (migration) assays, matrigel invasion assays, and proliferation assays. (biomedcentral.com)
  • SPARC (secreted protein,acidic and rich in cysteine) is a matricellular glycoprotein that regulates cell-cell and cell-matrix interactions, cellular proliferation and differentiation, and the expression of genes encoding extracellular matrix components. (silverchair.com)
  • By contrast, the absence of SPARC had no apparent morphological effect on the early stages of lens formation, cell proliferation or fiber cell differentiation. (silverchair.com)
  • In addition to providing structural integrity, ECM delivers important spatial and contextual cues to drive varied behaviors such as proliferation, migration, epithelial-to-mesenchymal transition, apoptosis, and production of various secreted mediators (as reviewed in References 1 - 3 , among others). (atsjournals.org)
  • Hamilton: B. C. Decker Inc.). A number of anti-angiogenic activities have been reported for this protein, such as inhibition of endothelial cell proliferation, migration, and tube formation. (justia.com)
  • Proliferation Phase- Rebuilding of the tissue begins with the recruitment of varied cell types. (zennutrients.com)
  • Matrix metalloproteases (MMPs) are zinc-dependent proteinases that participate in extracellular matrix degradation 1-3 and favor the invasion and proliferation of tumor cells 4 . (bvsalud.org)
  • Some of the most enriched biological processes were lymphocyte activation and proliferation, leukocyte adhesion and migration, antigen processing and presentation and cellular response to IFN-γ. (sciencegate.app)
  • We also show that Col4a1 and Col4a2 mutants displayed disrupted myofibroblast proliferation, differentiation and migration. (biomedcentral.com)
  • Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
  • In addition to its role in the formation of epithelium and vasculature, type IV collagen appears to be key for alveolar myofibroblast development by inducing their proliferation, differentiation and migration throughout the developing septum. (biomedcentral.com)
  • The formation of the mammalian respiratory unit is initiated during the transition between the pseudoglandular and canalicular/saccular stages and is characterized by coordinated proliferation and differentiation of epithelial and mesenchymal cells. (biomedcentral.com)
  • It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. (lookformedical.com)
  • GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. (nature.com)
  • Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
  • Fibrosis is characterised by an excessive production of extracellular matrix components, and cells in the tissue are constantly interacting with these extracellular matrix proteins. (bmj.com)
  • Ceturegel™ Matrix LDEV-Free Matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. (yeasenbiotech.com)
  • We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
  • Although the mechanisms mediating the effects of the extracellular matrix (ECM) on breast carcinoma development in vivo are largely unknown, contributing factors may be adhesion mediated signaling and mechanical signals imparted on mammary epithelial cells from surrounding type-I collagen-rich stroma, either directly or across basement membrane proteins. (biomedcentral.com)
  • Other than water, the mesoglea is composed of several substances including fibrous proteins like collagen and heparan sulphate proteoglycans. (pearltrees.com)
  • An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. (embl.de)
  • Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. (embl.de)
  • Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
  • Aquaporins (AQP) are water channel proteins that enable fluid fluxes across cell membranes, important for homeostasis of the tissue environment and for cell migration. (biomedcentral.com)
  • Mammalian aquaporins are a family of 13 classes of intrinsic membrane proteins that assemble as tetramers (~30 kDa per subunit) and are known for their role in fluid homeostasis and trans-membrane transport of water and other small solutes [ 6 , 7 ]. (biomedcentral.com)
  • In fact, by mass, the most abundant proteins in our bodies are ECM proteins - the nonliving material outside of our cells that provides a physical scaffold, barrier, and mechanical and biological signaling. (mimetas.com)
  • This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
  • 2-5 The basement membrane provides a substrate that promotes epithelial cell adhesion, migration, and differentiation 5-7 and prevents epithelial cell apoptosis. (touchophthalmology.com)
  • At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties, and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. (yeasenbiotech.com)
  • The three-dimensional culture matrix formed by Matrix can promote the adherence and differentiation of epithelial cells, hepatocytes, sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. (yeasenbiotech.com)
  • Ceturegel™ Matrix also supports the regeneration of peripheral nerves and the differentiation of bovine fallopian tube epithelial cells. (yeasenbiotech.com)
  • The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
  • Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
  • These newly available materials led to compelling evidence that the physical cellular environment is far from inert, but acts as a biological and mechanical signal to regulate cell-type specific function, differentiation, and morphology 1 . (mimetas.com)
  • The extracellular matrix (ECM), the noncellular component of the microenvironment, influences cell growth, survival, migration and tissue-specific differentiation through a repertoire of receptors including integrins, syndecans and discoidin receptors. (ucsf.edu)
  • Accordingly, to clarify the molecular basis of tissue-specific differentiation and disease, a variety of 3D systems exploiting natural ECMs have been developed, such as reconstituted basement membrane (rBM) and purified collagen hydrogels. (ucsf.edu)
  • Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord[J]. Dev Neurosci, 2005, 27(1):20-26. (sdu.edu.cn)
  • Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
  • In this stage, mammary gland development depends on systemic (and maternal) hormones, but is also under the (local) regulation of paracrine communication between neighboring epithelial and mesenchymal cells by parathyroid hormone-related protein (PTHrP). (wikipedia.org)
  • As a basement membrane protein, COL4A1 is commonly expressed in many tissues and cell types. (cusabio.com)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
  • Using a new method for construction and database searches of sequence consensus strings, we have identified a new superfamily of protein modules comprising laminin G, thrombospondin N and the pentraxin families. (embl.de)
  • Vinculin, a plasma protein, links fibers to talin, which is a cell membrane protein. (medscape.com)
  • The major structural protein of basement membrane, collagen type IV, in the lens capsule was substantially altered in the absence of SPARC. (silverchair.com)
  • d - i Type IV collagen protein and mRNA expression in the lung. (biomedcentral.com)
  • At the same time, Ceturegel TM Matrix can be used as a basic scaffold for various tumor cell invasion studies using matrigel, an essential matrix for in vitro and in vivo studies of angiogenesis, and a three-dimensional scaffold for the growth of transplanted tumor cells in in vivo animal models. (yeasenbiotech.com)
  • Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
  • Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
  • Our hypothesis is that inhibition of AQP1 will reduce migration and invasiveness of colon cancer cells, and the migration and tube-forming capacity of endothelial cells in vitro . (biomedcentral.com)
  • By using human lungs and cells, this new system provides a novel way to culture cells in vitro to investigate matrix contributions to lung pathology in a disease-specific manner. (atsjournals.org)
  • Along with microfluidic or organ-on-a-chip systems , 3D cell culture offers researchers an unparalleled ability to recreate physiological compositions and spatial arrangements of cells in vitro . (mimetas.com)
  • Niclosamide effectively downregulated the sorafenib-induced gene expression associated with glycolysis (GLUT1, HK2, LDHA, and PEPCK), stemness (OCT4), and drug resistance (ABCG2) and enhanced the ability of sorafenib to reduce the mitochondrial membrane potential in vitro. (bvsalud.org)
  • The cytocompatibility of PLGA scaffolds coated with different concentration of chitosan with schwann cells in vitro[J]. Chin J Aesth Med, 2009, 18(11):1627- 1631. (sdu.edu.cn)
  • Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)
  • These specific components of the ECM are present in basement membranes and interstitial matrix surrounding and within the islets. (npod.org)
  • The IHC tests showed that the ECM of the mice testicular interstitial tissue showed reaction to anti-laminin-α5 antibody. (biomedres.info)
  • Kruskal Wallis test showed no significant difference in laminin α5 expression in the ECM of the interstitial spaces of seminiferous tubules between the groups. (biomedres.info)
  • High concentration of sodium nitrate did not change the laminin α5 expression in the ECM of the testicular interstitial tissue. (biomedres.info)
  • The high salt concentration due to osmotic pressure leads to the migration of the interstitial fluid into the ground substance. (biologywise.com)
  • A fibrillar collagen found primarily in interstitial CARTILAGE. (lookformedical.com)
  • Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • Our focus is on the ECM molecules heparan sulfate/heparan sulfate proteoglycans, hyaluronan and molecules that associate with hyaluronan, laminins, and collagens, as well as on the enzymes that can modulate these ECM components. (npod.org)
  • It not only functions as a physical scaffolding, but also provides a channel for the migration and communication of cells via the use of signaling molecules. (biologywise.com)
  • The cells embedded within it interact with the matrix as well as with other cells due to the presence of specialized matrix receptor molecules. (biologywise.com)
  • These molecules interact with the matrix as well as the internal processes of the cell, thereby, bringing about cellular exchange of signals. (biologywise.com)
  • Cells in vivo experience a complex, three-dimensional environment that exposes them to circulating molecules, neighboring cells, and the extracellular matrix (ECM). (mimetas.com)
  • OThe molecules of the extracellular matrix play an important role during cell development and morphogenesis. (bvsalud.org)
  • Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. (lookformedical.com)
  • The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
  • The laminin tyrosine-isoleucine-glycine-serine-arginine (YIGSR) peptide, corresponding to the 929-933 sequence of the β1 chain, is known to be a functional motif with effects on the inhibition of tumor metastasis, the regulation of sensory axonal response and the inhibition of angiogenesis through high affinity to the 67 kDa laminin receptor. (peptide.com)
  • Growing evidence suggests that neoantigen-based cancer vaccines possess a very promising future, yet the processes of immune recognition and activation to relay identification of a neoantigen through the histocompatibility complex (MHC) and T-cell receptor (TCR) remain unclear. (bvsalud.org)
  • However, 3D cell culture is far superior in providing a more physiological or in vivo -like response. (pharmasalmanac.com)
  • but the role of GPR56 in neuronal migration in vivo remains unknown. (jneurosci.org)
  • This mimics the in vivo conditions at the basement membrane in terms of physical and functional view. (szabo-scandic.com)
  • The cells behave more or less as if they were in vivo. (szabo-scandic.com)
  • Imaging of the tumor-stromal interface in live tumor tissue ex vivo was performed with multiphoton laser-scanning microscopy (MPLSM) to generate multiphoton excitation (MPE) of endogenous fluorophores and second harmonic generation (SHG) to image stromal collagen. (biomedcentral.com)
  • Meanwhile, 3D cell culture systems feature increased complexity for increased faithfulness to the in vivo environment. (mimetas.com)
  • HoxA9 represses the malignant phenotype of breast cancer cells in vivo as well as in 3D rBM and this reversion is coincident with BRCA1 induction and normalization of adhesion and integrin expression. (ucsf.edu)
  • Laminin peptide YIGSR induces collagen synthesis in Hs27 human dermal fibroblasts. (peptide.com)
  • Phosphorylated osteopontin secreted from cancer cells induces cancer cell motility. (fmu.ac.jp)
  • Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse[J]. J Exp Med, 2005, 201(9):1397-1405. (sdu.edu.cn)
  • Mammary epithelial ECM mainly contains myoepithelial basement membrane and the connective tissue. (wikipedia.org)
  • These mesenchymal cells can transform into a dense, mammary-specific mesenchyme, which later develop into connective tissue with fibrous threads, forming blood vessels and the lymph system. (wikipedia.org)
  • Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), and nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors contained in EHS tumors. (yeasenbiotech.com)
  • A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
  • Sodium nitrite causes the irritation of evolving male germ cells by disrupting hormonal balance and tissue anoxia, and by deforming the sperms, causes the reduction of their fertility. (biomedres.info)
  • Moreover, collagen-dense breast tissue increases the risk of breast carcinoma, although the relationship between collagen density and tumorigenesis is not well understood. (biomedcentral.com)
  • One important step to elucidating these signaling interactions is to determine the organization of the collagenous stroma surrounding both normal mammary glands and tumors within intact tissue so as to better understand the cell-matrix interaction and how matrix organization, density, and composition affect tumor formation and progression. (biomedcentral.com)
  • The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
  • GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
  • Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
  • Numerous cytokines and growth factors that are up-regulated in corneal cells further contribute to tissue inflammation. (medscape.com)
  • The extracellular matrix (ECM) acts as an adhesive that holds all the cells of a tissue in place. (biologywise.com)
  • Expression of AQP1 in cell lines was assessed by quantitative (q) PCR, western blot and immunofluorescence, while expression of AQP1 in human colon tumour tissue was assessed by immunohistochemistry. (biomedcentral.com)
  • Extracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. (atsjournals.org)
  • The primary changes to cells in 3D are rather obvious - shape, motility, and polarity are all affected - but can have important secondary, less-obvious tissue-specific consequences. (mimetas.com)
  • An understanding of how biomechanical and biochemical signals influence directional migration to these sites of tissue escape could help develop therapies to slow or halt metastasis. (ucsf.edu)
  • Genetic and epigenetic changes in mammary epithelial cells (MECs) cooperate with a modified tissue microenvironment to drive malignant transformation of the breast. (ucsf.edu)
  • 9] Alessandri G, Emanueli C, Madeddu P. Genetically engineered stem cell therapy for tissue regeneration[J]. Ann N Y Acad Sci, 2004, 1015:271-284. (sdu.edu.cn)
  • Although the catalytic domain of MMPs is structurally highly similar, there are many differences with respect to substrate specificity, cellular and tissue localization, membrane binding and regulation that make this a very versatile family of enzymes with a multitude of physiological functions, many of which are still not fully understood. (evelinvahter.com)
  • Most likely, complex interaction between resident and immune/inflammatory cells results in release of proteolytic enzymes capable of destroying lung tissue and leading to emphysema. (atsjournals.org)
  • A family of structurally related collagens that form the characteristic collagen fibril bundles seen in CONNECTIVE TISSUE. (lookformedical.com)
  • A non-fibrillar collagen that forms a network of MICROFIBRILS within the EXTRACELLULAR MATRIX of CONNECTIVE TISSUE. (lookformedical.com)
  • This classification was based on the notion that "collagen" was equivalent to "connective tissue", but with the present recognition of the different types of collagen and the aggregates derived from them as distinct entities, the term "collagen diseases" now pertains exclusively to those inherited conditions in which the primary defect is at the gene level and affects collagen biosynthesis, post-translational modification, or extracellular processing directly. (lookformedical.com)
  • Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. (lookformedical.com)
  • A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. (lookformedical.com)
  • 4] Sorenson E J, Windebank A J. Relative importance of basement membrane and soluble growth factors in delayed and immediate regeneration of rat sciatic nerve[J]. J Neuropathol Exp Neurol, 1993, 52(3):216-222. (sdu.edu.cn)
  • MMPs can be grouped into soluble (including matrilysins) and membrane-anchored species. (evelinvahter.com)
  • Ligation of integrin α2β1 on collagen prevents flow-induced NF-κB activation through a p38-dependent pathway that is activated locally at adhesion sites. (rupress.org)
  • Endostatin inhibits endothelial cell migration by inhibiting phosphorylation of focal adhesion kinase via binding to α5β1 integrin (Wickstrom et al. (justia.com)
  • Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma. (fmu.ac.jp)
  • We investigate how mechanical and topological properties of the ECM, which are related to its composition and organization, alter integrin expression and function to modify stem cell fate and tumor progression. (ucsf.edu)
  • In particular, local cell invasion was found predominantly to be oriented along certain aligned collagen fibers, suggesting that radial alignment of collagen fibers relative to tumors facilitates invasion. (biomedcentral.com)
  • The presentation of these tumor-associated collagen signatures allowed us to identify pre-palpable tumors and see cells at the tumor-stromal boundary invading into the stroma along radially aligned collagen fibers. (biomedcentral.com)
  • Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)
  • The dermal ECM is synthesized from fibroblasts and is primarily compromised of fibrillar collagen and elastic fibers, which support the mechanical strength and resiliency of skin, respectively. (peptide.com)
  • Local alterations in collagen density were clearly seen, allowing us to obtain three-dimensional information regarding the organization of the mammary stroma, such as radiating collagen fibers that could not have been obtained using classical histological techniques. (biomedcentral.com)
  • Consistent with this observation, primary tumor explants cultured in a randomly organized collagen matrix realigned the collagen fibers, allowing individual tumor cells to migrate out along radially aligned fibers. (biomedcentral.com)
  • The mesohyl includes a noncellular colloidal mesoglea with embedded collagen fibers, spicules and various cells, being as such a type of mesenchyme. (pearltrees.com)
  • Contraction of actin fibers pulls the cell body forward. (medscape.com)
  • Extracellular matrix is the extracellular, complex mixture of various biomolecules and fibers secreted by cells in the tissues of multicellular organisms. (biologywise.com)
  • Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
  • Mesoglea, also known as mesohyl, is the translucent, non-living, jelly-like substance found between the two epithelial cell layers (i.e., between the ectoderm and endoderm) in the bodies of cnidarians and sponges. (pearltrees.com)
  • After 24-30 hours, mitosis begins to restore epithelial cell population. (medscape.com)
  • To understand epithelial cell biology requires defined biomaterials in which biochemical, topological and biophysical properties can be systematically varied. (ucsf.edu)
  • Towards this goal we use the nonmalignant MCF10A mammary epithelial cell (MEC) line and are conducting a systematic analysis of acinar morphogenesis using the natural hydrogels collagen type I and rBM and three synthetic matrices: rBM-conjugated polyacrylamide gels, self assembling peptide gels (PuraMatrix) with and without rBM and hyaluronic acid gels with and without rBM. (ucsf.edu)
  • The representative image of HepG2 cell stained with crystal violet after the invasion (Figure 1A). (yeasenbiotech.com)
  • The Matrigel matrix thus provides the potential for studies of cell morphology, biochemical functions, migration or invasion, and gene expression. (szabo-scandic.com)
  • Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
  • This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
  • dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
  • These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
  • Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells (SCs)and neural stem cells(NSCs) as well as cells adhesion and migration on the arranged nanofibers inside the lumen. (sdu.edu.cn)
  • Scanning electron microscopy(SEM) was used to detect cells adhesion and migration on the axially arranged nanofibers inside the lumen. (sdu.edu.cn)
  • NO is reactive oxygen radical that is produced in testicular vascular endothelial cells and leydig cells, and by affecting the hypothalamicpituitarygonadal axis causes a reduction in testosterone production and inhibits steroidogenesis [ 5 , 6 ]. (biomedres.info)
  • Maintaining the correct polarized morphology of the lactiferous duct tree requires another essential component - mammary epithelial cells extracellular matrix (ECM) which, together with adipocytes, fibroblast, inflammatory cells, and others, constitute mammary stroma. (wikipedia.org)
  • AM is the innermost layer of the placenta and is composed of an epithelial monolayer, an underlying thick basement membrane, and an avascular stroma. (touchophthalmology.com)
  • At the site of insulitis, the ECM serves as a substrate for the trafficking of immune cells from the blood stream into the islet, but also acts as a barrier to inflammatory cells infiltrating the islet and leading to destruction of the β cells. (npod.org)
  • They are classified into 5 groups according to substrate specificity and internal homology: collagenases, gelatinases, stromelysins, membrane-type MMPs, and others, including matrilysins 8 . (bvsalud.org)
  • The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. (bmj.com)
  • It enables the investigation of cell-cell and cell-extracellular matrix (ECM) interactions. (pharmasalmanac.com)
  • Ceturegel™ Matrix can affect gene expression in three-dimensional cultures of murine hepatocytes and human mammary epithelial cells. (yeasenbiotech.com)
  • YEASEN Matrix is a sterile product with a concentration of 8~20 mg/mL, which meets a variety of experimental requirements, including angiogenesis studies and tumor cell migration. (yeasenbiotech.com)
  • The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
  • The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
  • This study aims to investigate the effects of oral administration of sodium nitrite on expression of laminin α5 in extracellular matrix of mice testicular interestitium using an Immunohistochemical (IHC) assessment. (biomedres.info)
  • Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
  • The main function of metalloproteinases is to degrade extracellular matrix and basement membrane components. (medscape.com)
  • The lens capsule, which is also called the lens basement membrane, is a specialized extracellular matrix produced anteriorly by the lens epithelium and posteriorly by newly differentiated fiber cells. (silverchair.com)
  • These results underscore the importance of the capsular extracellular matrix in the maintenance of lens transparency and indicate that SPARC participates in the synthesis, assembly and/or stabilization of the lens basement membrane. (silverchair.com)
  • SPARC, which is also termed osteonectin or BM-40, belongs to the matricellular class of secreted glycoproteins (thrombospondins 1 and 2,osteopontin, tenascins C and X) that function as modulators of cell-cell and cell-matrix interactions. (silverchair.com)
  • We define a new method to prepare acellular lung matrix that can be used to study the impact of extracellular matrix on lung cell phenotype. (atsjournals.org)
  • 3D cell culture represents yet another step towards this goal by focusing on mimicking normal cell-matrix and cell-cell interactions. (mimetas.com)
  • Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
  • To understand how the mechanical properties of the tumor microenvironment may affect metastasis, we study cell motility of mammary epithelia cells migrating on substrates with gradients of stiffness and with different matrix conjugation. (ucsf.edu)
  • Exploring functional links between matrix stiffness, micro RNAs and HoxA9 dependent regulation of BRCA1 and mammary cell survival and tumorigenesis. (ucsf.edu)
  • Defining the cells and proteinases responsible for destruction of lung extracellular matrix associated with cigarette smoking will be required for development of appropriate proteinase inhibitors for application in COPD. (atsjournals.org)
  • Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. (lookformedical.com)
  • With these environmental cues in mind, biologists have developed more physiologically accurate methods for culturing cells and tissues, including new media formulations and co-culture techniques. (mimetas.com)
  • Metastatic progression depends on the ability of tumor cells to migrate to nearby blood vessels and lymphatics before they can successfully metastasize and colonize into other tissues. (ucsf.edu)
  • A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains. (lookformedical.com)
  • Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)
  • MMP-1 (neutrophil collagenase) and MMP-8 (fibroblast or keratocyte collagenase) are involved in cleaving collagen types I, II, and III. (medscape.com)
  • Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture[J]. J Biomed Mater Res, 2002, 62(3):438-446. (sdu.edu.cn)
  • Specific classes of AQP channels have been implicated in enhanced migration, angiogenesis and metastasis in a variety of cancer types [ 8 , 9 ], prompting the suggestion that inhibitors of AQP channels might provide new tools for cancer therapy [ 8 ]. (biomedcentral.com)
  • These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
  • In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
  • Basal and limbal stem cells contribute to mitosis. (medscape.com)
  • Our experiments demonstrate that GPR56 is essential for proper morphogenesis of the rostral cerebellum and suggest that defects caused by loss of GPR56 arise from granule cell abnormalities in this region. (jneurosci.org)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • This delay is required for preparatory cellular changes prior to rapid cell movement. (medscape.com)
  • [ 1 ] Early stage head and neck squamous cell carcinoma (HNSCC) is treated relatively well with single-modality therapy (either surgery or radiation alone). (medscape.com)
  • Go to Imaging of Nasopharyngeal and Laryngeal Squamous Cell Carcinoma and Head and Neck Squamous Cell Carcinoma for complete information on these topics. (medscape.com)
  • There are three main subtypes of NSCLC which are adenocarcinoma (~40% of cases), squamous cell carcinoma (~25-30% of cases), and large-cell carcinoma (~10-15% of cases). (hindawi.com)
  • It is due to different mutations in collagen IV [ 1 ]. (hindawi.com)
  • These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)
  • Using mouse models, we discovered that mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. (biomedcentral.com)
  • ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)
  • 1 In Crohn's disease, smooth muscle cells are considered to be the predominant cells involved in the process of stricture formation. (bmj.com)
  • AM J RESPIR CRIT CARE MED 1999;160:S29−S32.Macrophages are the predominant defense cell in the normal lung and during conditions associated with chronic inflammation such as COPD. (atsjournals.org)
  • METHODS Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. (bmj.com)
  • With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
  • The goal of specific molecular targets in cancer therapy is to create a "magic bullet" that selectively kills cancer cells. (medscape.com)
  • As our understanding of the molecular biology of HNSCC continues to develop, we can target the specific components of cancer cells that are not found in normal cells. (medscape.com)
  • Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
  • 1992]. Molecular mechanisms by which DCN interacts with TSP and inhibits cell adhesion to TSP are unknown. (embl.de)
  • Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid. (fmu.ac.jp)
  • My group is exploring the molecular mechanisms whereby ECM receptors modulate cell fate and tumorigenesis. (ucsf.edu)
  • The second part of our research is directed towards characterizing the role of and molecular mechanisms whereby mechanical force regulates human embryonic stem cell fate using 2D/3D natural and synthetic matrices and a force reactor with a focus on adhesion signaling and epigenetics. (ucsf.edu)
  • 3D cell culture offers tremendous promise to revolutionize drug discovery, disease modeling, and regenerative medicine by providing a platform that better recapitulates human biology than conventional 2D cell culture, leading to more accurate predictions of drug efficacy and toxicity and improved understanding of disease mechanisms. (pharmasalmanac.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • The effect of AqB013 on angiogenesis was tested using an endothelial cell tube-formation assay. (biomedcentral.com)
  • Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)
  • Inflammatory Phase- Immune cells migrate to the wound to clear the wound of debris and bacterial infection. (zennutrients.com)
  • 7 , 8 In systemic mast cell disease, association of fibrosis and mast cell infiltrates has been documented in the liver, spleen, lymph nodes, and bone marrow. (bmj.com)
  • 12-14 AM has also been shown to induce apoptosis of inflammatory cells. (touchophthalmology.com)
  • T1D results from an immune cell-mediated destruction of the pancreatic β cells, which takes place in a permissive inflammatory environment. (npod.org)
  • migration in different inflammatory models. (123dok.net)
  • Other immune and inflammatory cells including neutrophils, T lymphocytes, eosinophils, and mast cells are likely also to contribute to proteolytic injury. (atsjournals.org)
  • On the other hand, the heparin binding properties of endostatin manifest themselves in its interaction with the cell surface. (justia.com)
  • Attachment of human marrow stromal cells to titanium surfaces. (sciepub.com)
  • The basement membrane is also important for epithelial migration, and abnormalities in basement membrane structure, whether due to trauma (eg, recurrent erosion syndrome) or dystrophy (eg, basement membrane dystrophy), can lead to persistence of corneal epithelial defects and stromal ulceration. (medscape.com)