Basement laminin. Medical search. Frequent questions

Immunostaining and confocal fluorescence microscopy show that the ECM proteins fibronectin, collagen I, and laminin are deposited throughout the entire 3D structure. (nature.com)
Distribution of type IV collagen and laminin. (nih.gov)
19. The spatial and temporal expression of the alpha 2 beta 1 integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis. (nih.gov)
In intact skin, laminin-5 provides essential links to both the hemidesmosomal alpha6beta4 integrin and the collagen type VII molecules which form the anchoring fibrils inserting into the dermis. (nih.gov)
Geltrex matrix is a basement membrane extract that contains laminin, collagen IV, entactin, and heparin sulfate proteoglycans. (thermofisher.com)
Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (embl.de)
Binding sites for laminin and collagen IV are present on the C-terminal globule but not yet precisely localized. (embl.de)
The National Institute of Dental Research (NIDR) has a requirement to furnish the necessary services, personnel, material, equipment, and facilities, not otherwise provided by the Government, to prepare and deliver Laminin-1-sterile, Collagen IV, and EHS tumor. (nih.gov)
Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via type I hemidesmosomes and integrin β1-based adhesion complexes. (nki.nl)
To date, there has been no valid hypothesis of why renal, auditory, and ocular manifestations are observed in Alport's syndrome, but other basement membrane beds involving alpha-5 type IV collagen are no involved, such as the epidermal basement membrane of the skin. (wikidoc.org)

Immunofluorescence clearly localised laminin and fibronectin in the basement membranes of ocular epithelia through all stages of rat lens differentiation. (nih.gov)
The unlabelled peroxidase anti-peroxidase (PAP) method shows that 'blebs' and 'blisters' of basement membranes, particularly from the optic vesicle, appear to give rise to cords of fibronectin- and laminin-positive material. (nih.gov)
He is known for his work on laminins - huge proteins that are a key component of all basement membranes. (imperial.ac.uk)
We identified unique expression patterns of laminin proteins in high endothelial venule basement membranes and the cortical ridge that correlated with alloantigen-specific immunity or immune tolerance. (jci.org)
They are also involved in the formation and organization of basement membranes, which are thin, sheet-like structures that separate and support cells in many tissues. (medlineplus.gov)
Vertebrate nidogen-1 (NID-1) or entactin, a sulphated glycoprotein widely distributed in basement membranes. (embl.de)
Vertebrate nidogen-2 (NID-2) or osteonidogen, a cell adhesion glycoprotein which is widely distributed in basement membranes. (embl.de)
Basement membranes can help determine pathways of migrating axons. (embl.de)
Although members of the nidogen (entactin) protein family are structural components of basement membranes, we find that nidogen is not required for basement membrane assembly in the nematode Caenorhabditis elegans. (embl.de)
Nidogen is localized to body wall basement membranes and is required to direct longitudinal nerves dorsoventrally and to direct axons at the midlines. (embl.de)
Specialized basement membranes may thus regulate nerve position. (embl.de)
Nidogen, a multifunctional glycoprotein, is an integral part of all basement membranes. (embl.de)
Laminins [ ( PUBMED:2404817 ) ] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. (embl.de)
1982). The distribution of laminin did not change during the rejection process and was localized in the basement membrane zone of myofibers and myotubes, although the overall configuration of the basement membranes was deformed and collapsed. (zib.de)
It appears that the basement membranes are resistant to degradation, and staining for laminin persists in rejected muscle. (zib.de)
They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. (icosagen.com)
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (nih.gov)
Laminin is a glycoprotein that is a major component of the extracellular matrix and is essential for the formation of basement membranes. (atlasbars.com)
Basement membranes are thin, sheet-like structures that separate and support cells in various tissues, including the skin, lungs, and kidneys. (atlasbars.com)
In the adrenal gland and kidney, INO selectively reacts with areas rich in basement membranes, staining a subset of structures that are immunoreactive for both laminin and heparan sulfate proteoglycan. (silverchair.com)
Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. (utsouthwestern.edu)
PHENOTYPE: Embryos homozygous for a gene trapped allele lack basement membranes and fail to survive past E5.5. (utsouthwestern.edu)

Later, as the mesoderm is excluded and the lens placode invaginates to form the lens pit, there is a marked increase in the concentration of both laminin and fibronectin in the interspace. (nih.gov)
It is suggested that, in addition to providing good adhesion between the tissues, this laminin- and fibronectin-rich ECM may stimulate the formation of basal extensions and cytoplasmic processes, particularly from the lens placode, and therefore, initiate the ectoderm to form lens placode. (nih.gov)
Secreted molecules also contribute to the extracellular matrix (fibronectin, laminin, reelin). (frontiersin.org)
In the present study, changes in two extracellular matrix glycoproteins, fibronectin and laminin, were investigated in skeletal muscle undergoing immune rejection. (zib.de)
Purified antibodies against fibronectin and laminin were used to analyze the matrix by indirect immunofluorescence at various intervals after transplantation of extensor digitorum longus muscle in rats. (zib.de)

A cDNA clone encoding the murine laminin receptor was isolated and identified on the basis of sequence homology to the human laminin receptor [Wewer et al. (nih.gov)

16. Basement membrane proteins in salivary gland tumours. (nih.gov)
Extracellular matrix proteins, including those of the laminin family, formed regions within the LN that were permissive for colocalization of alloantigen-presenting cells, alloreactive T cells, and Tregs. (jci.org)
Laminins are a group of proteins that regulate cell growth, cell movement (motility), and the attachment of cells to one another (adhesion). (medlineplus.gov)
Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. (icosagen.com)

Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. (wikipedia.org)
The LAMC2 gene provides instructions for making one part (subunit) of a protein called laminin 332 (formerly known as laminin 5). (medlineplus.gov)
Most of these mutations lead to a premature stop signal in the instructions for making the gamma subunit of laminin 332, which prevents the assembly of this protein. (medlineplus.gov)
Some of these mutations alter single protein building blocks (amino acids) in the gamma subunit of laminin 332. (medlineplus.gov)

14. Laminin isoform profiles in salivary glands in Sjögren's syndrome. (nih.gov)
An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. (icosagen.com)
This gene encodes the beta chain isoform laminin, beta 1. (utsouthwestern.edu)

After 96 h, SM was found to induce skin injury characterized by edema, epidermal hyperplasia, loss of the differentiation marker, keratin 10 (K10), upregulation of the skin wound marker keratin 6 (K6), disruption of the basement membrane anchoring protein laminin 322, and increased expression of epidermal COX2. (nih.gov)
He found that disruption of the basement membrane is the general hallmark of the transition from in situ to invasive cancer for all human epithelial cancers. (nih.gov)

Laminin, an extracellular matrix protein, is a major component of the basement membrane. (wikipedia.org)

This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). (wikipedia.org)
Mutations of the laminin-5 genes or auto-antibodies against one of the subunits of laminin-5 may lead to a significant lack of this molecule in the epidermal basement membrane zone. (nih.gov)

Indirect immunofluorescence study performed on salt-split normal human skin substrate with the serum from a patient with bullous pemphigoid detects immunoglobulin G class circulating autoantibodies that bind to the epidermal (roof) side of the skin basement membrane. (medscape.com)
IDIF studies document the presence of circulating IgG autoantibodies in the patient's serum that target the skin basement membrane component. (medscape.com)
[ 5 ] In patients with BPP, serum autoantibodies may react with components of the basement membrane. (medscape.com)
Recently, autoantibodies to Laminin-332 have been described in several autoimmune diseases. (bvsalud.org)
The importance of Laminin-332 autoantibodies and its relationship to malignancy is highlighted by using Laminin-332 Pemphigoid (LM-332Pg) as a prototype. (bvsalud.org)
OBJECTIVE: To identify several autoimmune diseases that have autoantibodies to Laminin-332 present, and to determine the prevalence of malignancy in them. (bvsalud.org)
By identifying the temporal detection of cancer, can the influence of autoantibodies to Laminin-332 on prognosis be determined. (bvsalud.org)
EVIDENCE REVIEW: A literature search was conducted to identify autoimmune and inflammatory diseases in which autoantibodies to Laminin-332 were present. (bvsalud.org)
FINDINGS: Autoantibodies to Laminin-332 were detected in recent studies of systemic lupus erythematosus (SLE), psoriasis, bronchiolitis obliterans (BO), graft-vs-host disease (GVH), bullous pemphigoid (BP), lichen planus (LP), epidermolysis bullosa acquisita (EBA), and membranous glomerulonephropathy (MGN). (bvsalud.org)
When studied, levels of Laminin-332 autoantibodies correlated with the presence or absence of malignancy. (bvsalud.org)

We report the crystal structure at 2.13 Å resolution of a minimal integrin-binding fragment of mouse laminin-111, consisting of ∼50 residues of α1β1γ1 coiled coil and the first three laminin G-like (LG) domains of the α1 chain. (nih.gov)
Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. (icosagen.com)

The more serious form of the disease, known as JEB generalized severe, usually results from mutations that prevent the production of functional laminin 332. (medlineplus.gov)
Muhle C, Jiang QJ, Charlesworth A, Bruckner-Tuderman L, Meneguzzi G, Schneider H. Novel and recurrent mutations in the laminin-5 genes causing lethal junctional epidermolysis bullosa: molecular basis and clinical course of Herlitz disease. (nih.gov)

Integrins are important laminin receptors, but their binding site on the heterotrimeric laminins is poorly defined structurally. (nih.gov)

The basement membrane glycoprotein laminin-5 is a key component of the anchoring complex connecting keratinocytes to the underlying dermis. (nih.gov)

These are likely to be laminin and heparan sulfate proteoglycan, respectively. (silverchair.com)

Gibco Geltrex Matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from murine Engelbreth-Holm-Swarm tumor. (thermofisher.com)

Molecular detection of noninvasive and invasive bladder tumor tissues and exfoliated cells by aberrant promoter methylation of laminin-5 encoding genes. (nih.gov)
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. (nih.gov)

Furthermore, reducing α4 laminin circumvented tolerance induction and induced cardiac allograft inflammation and rejection in murine models. (jci.org)
Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. (nih.gov)
Primer extension experiments demonstrated that the clone contained the complete 5' sequence of the murine laminin receptor mRNA. (nih.gov)

Laminin-5 (LN5) anchors epithelial cells to the underlying basement membrane, and it is encoded by three distinct genes: LAMA3, LAMB3, and LAMC2. (nih.gov)

In 1975 he proposed that tumor cell attachment and degradation of the basement membrane (a collagenous sheath that surrounds epithelial ducts, blood vessels and nerves, and separates tissue compartments) was crucial to invasion and metastasis. (nih.gov)
The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. (nih.gov)

Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (utsouthwestern.edu)

The precursor of the laminin receptor is apparently smaller than the 67-kilodalton protein isolated from tissue. (nih.gov)

The pathophysiology of DKD comprises the interaction of both genetic and environmental determinants that trigger a complex network of pathophysiological events, which leads to the damage of the glomerular filtration barrier, a highly specialized structure formed by the fenestrated endothelium, the glomerular basement membrane and the epithelial podocytes, that permits a highly selective ultrafiltration of the blood plasma. (wiley.com)
The shutdown of these chains results in immature development of the glomerular basement membrane, as α-3,α-4,α-5 chains are essential in producing a mature and well functioning glomerular basement membrane. (wikidoc.org)
are still being produced in the glomerular basement membrane. (wikidoc.org)
The production of only these chains in the glomerular basement membrane results in the glomerular basement membrane to be prone to endoproteolysis and oxidative stressors. (wikidoc.org)
As time progresses, the renal under constant oxidative stressors and endoproteolysis conforms the glomerular basement membrane to become unevenly thick, split, and unfortunately damaged in a way that it is no longer able to function properly. (wikidoc.org)
Involvement of the glomerular basement membrane is the hallmark of Alport's syndrome. (wikidoc.org)

The major contributions of laminin-5 to the resistance of the epidermis against frictional stress but also for basement membrane regeneration and repair of damaged skin are reflected by the phenotype of Herlitz junctional epidermolysis bullosa, which is caused by an inherited absence of functional laminin-5. (nih.gov)
Laminin 332 has a particularly important role in the basement membrane that underlies the top layer of skin (the epidermis). (medlineplus.gov)
Without laminin 332, the epidermis is only weakly connected to the underlying layers of skin. (medlineplus.gov)

Hartwig B, Borm B, Schneider H, Arin MJ, Kirfel G, Herzog V. Laminin-5-deficient human keratinocytes: defective adhesion results in a saltatory and inefficient mode of migration. (medlineplus.gov)

Laminin 332 is a major component of fibers called anchoring filaments, which connect the two layers of the basement membrane and help hold the skin together. (medlineplus.gov)

IMPORTANCE: Laminin-332 is an important component of the basement membrane. (bvsalud.org)
The name Laminine comes from "laminin" - a component of our body cells, and the ending of the name (nine - 9) symbolizes the day when the most important ingredient is extracted from fertilized bird eggs. (imed.guru)

INO also binds to the basement membrane surrounding cardiac and skeletal muscle cells, and binding to the latter greatly increases after denervation. (silverchair.com)

The basement membrane is a thin sheet of fibers that underlies the epithelium, which lines the cavities and surfaces of organs including skin, or the endothelium, which lines the interior surface of blood vessels. (loinc.org)
These molecules form the vascular matrix and subendothelial basement membrane of blood vessels [ 2 ]. (biomedcentral.com)

20. Chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components. (nih.gov)

Reactivity of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions). (icosagen.com)
Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (nih.gov)
Each laminin chain is a multidomain protein encoded by a distinct gene. (nih.gov)
A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. (utsouthwestern.edu)

These chains are essential in forming the basement membrane. (wikidoc.org)
Through combination, laminin chains constitute 15 laminin isoforms. (icosagen.com)

The nascent laminin receptor predicted from the cDNA sequence is 295 amino acids long, with a molecular weight of 33,000, and contains one intradisulfide bridge, a short putative transmembrane domain, and an extracellular carboxy-terminal region which has abundant glutamic acid residues and multiple repeat sequences. (nih.gov)

Inhalation exposure of rats to diacetyl or 2,3-pentanedione, a related flavoring agent, can cause severe injury to the airway epithelium and underlying basement membrane. (nih.gov)

as the lens capsule thickens, the fluorescence for laminin increases in the latter. (nih.gov)

The apparent molecular weight on SDS-polyacrylamide gels of the rabbit reticulocyte cell-free translation product of selectively hybridized laminin receptor mRNA is 37,000. (nih.gov)

PREPARATION OF BASEMENT MEMBRANE REAGENT COMPONENTS NIH GUIDE, Volume 25, Number 15, May 10, 1996 RFP AVAILABLE: NLM-96-108/VMS P.T. 34 Keywords: Biological Resources National Library of Medicine This procurement is a 100 percent set-aside for small businesses. (nih.gov)

Laminins are cell-adhesive glycoproteins that are essential for basement membrane assembly and function. (nih.gov)

[ 8 ] Given that the patient in this case did not have IgA basement membrane zone antibodies nor any recent changes in medications, LABD is less likely. (medscape.com)

If the basement membrane is injured, laminin-5 production increases rapidly. (nih.gov)

Note the discontinuity of the basement membrane between the arrowheads, associated with proliferation of fibrous tissue that has protruded through the defect to form a fibrous polyp (asterisk) in the bronchial lumen. (nih.gov)

It then serves as a scaffold for cell migration, initiates the formation of hemidesmosomes and accelerates basement membrane restoration at the dermal-epidermal junction. (nih.gov)
The ratio of laminin α4 to laminin α5 was greater in domains within tolerant LNs, compared with immune LNs, and blocking laminin α4 function or inducing laminin α5 overexpression disrupted T cell and DC localization and transmigration through tolerant LNs. (jci.org)
Also functions as a cell surface receptor for laminin. (joplink.net)
Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. (joplink.net)
Cross-shaped, Laminin plays an important role in cell differentiation, migration and cell adhesion. (imed.guru)

Additionally, researchers have proposed roles for laminin 332 in the clear outer covering of the eye (the cornea) and in the development of tooth enamel. (medlineplus.gov)

The primary function of the basement membrane is to anchor down the epithelium to its loose connective tissue underneath. (loinc.org)
Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. (loinc.org)
Another important protein that aids in tissue repair is laminin. (atlasbars.com)

Laminin is an important protein in the cell's basement membrane, which is the basic structure of most of our cells and organs. (imed.guru)

Anatomy24

Organisms1

Diseases12

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes11

Disciplines and Occupations1

Information Science3

Collagen10

Membranes22

Fibronectin5

Human laminin1

Proteins4

Subunit4

Isoform3

Disruption of the basement membrane2

Extracellular matrix1

Subunits2

Autoantibodies10

Integrin2

Mutations2

Receptors1

Glycoprotein1

Heparan Sulfate1

Engelbreth-Holm-Swarm1

Tissues2

Murine3

Epithelial Cells1

Tumor2

Heterotrimeric1

Precursor1

Glomerular basement6

Epidermis3

Keratinocytes1

Layers of the basement membrane1

Component2

Skeletal muscle1

Blood vessels2

Regulating the basement membrane1

Chain4

Chains2

Residues1

Rats1

Lens capsule1

Selectively1

Biological1

Membrane assembly1

Antibodies1

Increases1

Proliferation1

Cell5

Roles1

Tissue3

Important1