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  • possible
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • presence
  • We now know that this effect is due to increased stacking interactions between GC base pairs (and not the presence of three hydrogen bonds) [ DNA Denaturation and Renaturation and the Role of Hydrogen Bonds and Stacking Interactions ]. (blogspot.ca)
  • mutations
  • Based on Hadoop and HBase, we developed SeqHBase, a big data-based toolset for analysing family based sequencing data to detect de novo, inherited homozygous, or compound heterozygous mutations that may contribute to disease manifestations. (bmj.com)
  • The remaining 8 were new mutations and all were single base changes: Four were in families that are strongly associated with cancer pathogenesis (PTPRT, CDH24, PCLKC and SLC15A1). (wikipedia.org)
  • The initiative allows such exceptional patients (who have responded positively for at least six months to a cancer drug that usually fails) to have their genomes sequenced to identify the relevant mutations. (wikipedia.org)
  • Conserved sequences, such as mitochondrial DNA, are expected to accumulate mutations over time, and assuming a constant rate of mutation, provides a molecular clock for dating divergence. (wikipedia.org)
  • contiguous
  • Results show that the use of exponential decay weighting schemes when taking into account non contiguous sequences to compute recommendations enhances the accuracy. (inria.fr)
  • A comprehensive step-by-step protocol on constructing phylogenetic tree, including DNA/Amino Acid contiguous sequence assembly, multiple sequence alignment, model-test (testing best-fitting substitution models) and phylogeny reconstruction using Maximum Likelihood and Bayesian Inference, is available at Nature Protocol Early attempts at molecular systematics were also termed as chemotaxonomy and made use of proteins, enzymes, carbohydrates, and other molecules that were separated and characterized using techniques such as chromatography. (wikipedia.org)
  • protein
  • tRNA does this by carrying an amino acid to the protein synthetic machinery of a cell (ribosome) as directed by a three-nucleotide sequence (codon) in a messenger RNA (mRNA). (wikipedia.org)
  • In 1999, working with John Carlson at Yale, he developed a novel algorithm for computationally identifying G Protein-Coupled Receptors (GPCR) without using primary sequence homology, which led to the cloning of olfactory receptors in insects. (wikipedia.org)
  • molecular
  • Molecular phylogenetics (/məˈlɛkjʊlər ˌfaɪloʊdʒəˈnɛtɪks, mɒ-, moʊ-/) is the branch of phylogeny that analyses hereditary molecular differences, mainly in DNA sequences, to gain information on an organism's evolutionary relationships. (wikipedia.org)
  • With the invention of Sanger sequencing in 1977 it became possible to isolate and identify these molecular structures. (wikipedia.org)
  • Its molecular structure has DNA bases attached to a backbone of methylenemorpholine rings linked through phosphorodiamidate groups. (wikipedia.org)
  • canonical
  • We compare three sequentializations: a direct linearization of the logical form, a linearization of the associated canonical realization, and a sequence consisting of derivation steps relative to the underlying grammar. (inria.fr)
  • In the canonical Watson-Crick base pairing, adenine (A) forms a base pair with thymine (T) and guanine (G) forms one with cytosine (C) in DNA. (wikipedia.org)
  • Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix). (wikipedia.org)
  • The viral polymerase incorporates these compounds with non-canonical bases. (wikipedia.org)
  • These nucleoside triphosphates possess a non-canonical sugar, dideoxyribose, which lacks the 3' hydroxyl group normally present in DNA and therefore cannot bond with the next base. (wikipedia.org)
  • detect
  • DNA denaturation can also be used to detect sequence differences between two different DNA sequences. (wikipedia.org)
  • In the context of a single isolated region of DNA, denaturing gradient gels and temperature gradient gels can be used to detect the presence of small mismatches between two sequences, a process known as temperature gradient gel electrophoresis. (wikipedia.org)
  • homology
  • examined misidentification of VGS submitted to antimicrobial surveillance programs as pneumococci and found that the distinction of S. pneumoniae from the VGS can be difficult, which is not surprising in light of the fact that S. mitis and S. oralis possess >99% sequence homology with S. pneumoniae (47). (wikipedia.org)
  • next-generation
  • NEW YORK (GenomeWeb) - Investigators at the Scripps Research Institute, the International AIDS Vaccine Initiative, and the Ragon Institute of Massachusetts General Hospital have come up with a set of next-generation sequencing techniques for profiling antibody repertoires of immune B cells - collections that provide clues for treating and tracking some forms of infectious disease. (genomeweb.com)
  • Automated on the HTG EdgeSeq system, HTG EdgeSeq Immuno-Oncology Assay couples HTG's proprietary nuclease protection chemistry with next-generation sequencing (NGS). (cnbc.com)
  • HTG Edge system capabilities have been expanded to fully automate sample and targeted library preparation for next-generation sequencing. (cnbc.com)
  • The SRA was started by NCBI in 2007 to collect sequence data produced by next-generation sequencing instruments. (wikipedia.org)
  • restriction
  • citation needed] While a postdoctoral fellow with Hamilton O. Smith at the Johns Hopkins University School of Medicine during 1969-70, Kelly determined the DNA sequences recognized by type II restriction enzymes, which subsequently became major tools in recombinant DNA research. (wikipedia.org)
  • Sequencer
  • Our new State-Based Sequencer counters both of these limitations, using an agent-based collection of algorithms combined with a technique to provide motivation to the resultant music. (soton.ac.uk)
  • Novel DNA sequence determination method and DNA sequencer system providing a sequencing speed 10 3 to 10 4 times faster than the current DNA sequencing speed (10 5 bases per day with a lane at maximum) of the existing DNA sequencer based on electrophoresis. (freepatentsonline.com)
  • The present invention relates to a method and apparatus for determining the base sequence of a DNA or the like of biological or non-biological origin and, more particularly, to a so-called ultrahigh-speed DNA sequencer used to sequence the bases of DNA or the like at high speed. (freepatentsonline.com)
  • targets
  • The subjects were instructed to voluntarily control a virtual cursor in multiple trials to hit different pre-programmed targets on a screen in an optimized sequence. (springer.com)
  • Recommendations
  • This paper describes a new approach of generating suitable recommendations based on the active user's navigation stream. (inria.fr)
  • Recognition
  • EcoRII is a bacterial Type IIE REase that interacts with two or three copies of the pseudopalindromic DNA recognition sequence 5'-CCWGG-3' (W = A or T), one being the actual target of cleavage, the other(s) serving as the allosteric activator(s). (wikipedia.org)
  • This sequence is important for the recognition of tRNA by enzymes and critical in translation. (wikipedia.org)
  • user's
  • This approach is derived from a basic shared control strategy where the robotic arm is responsible for carrying out complex maneuvers based on the user's intention. (springer.com)
  • wobble
  • In RNA, thymine is replaced by uracil (U). Alternate hydrogen bonding patterns, such as the wobble base pair and Hoogsteen base pair, also occur-particularly in RNA-giving rise to complex and functional tertiary structures. (wikipedia.org)
  • The GU wobble base pair, with two hydrogen bonds, does occur fairly often in RNA. (wikipedia.org)
  • The first anticodon base, or wobble-position, is sometimes modified to inosine (derived from adenine), pseudouridine or lysidine (derived from cytosine). (wikipedia.org)
  • Some anticodons can pair with more than one codon due to a phenomenon known as wobble base pairing. (wikipedia.org)
  • Evolution
  • Jewell, Michael O. , Nixon, Mark S. and Prugel-Bennett, Adam (2005) State-Based Sequencing: Directing The Evolution of Music. (soton.ac.uk)
  • 1809 - Lamarck proposed a modern theory of evolution based on the inheritance of acquired characteristics. (wikipedia.org)
  • naturally
  • Naturally occurring bases can be divided into two classes according to their structure: pyrimidines are six-membered heterocyclic with nitrogen atoms in position 1 and 3. (wikipedia.org)
  • patterns
  • Recurrent Neural Networks (RNNs) have become the state-of-the-art choice for extracting patterns from temporal sequences. (arxiv.org)
  • https
  • https://bioinformatics.org/sms/ It contains all basic manipulations to a DNA sequence, it is implemented by javascript, and is web based, you can also download and have a local copy on you computer, it only need unzip, no installation. (bioinformatics.org)
  • typical
  • they also lack either the polysaccharide-based capsule typical of S. pneumoniae or the Lancefield antigens of the pyogenic members of the genus. (wikipedia.org)
  • different
  • If the 16S sequence is different, you can be sure that the isolates are different strains. (criver.com)
  • Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. (wikipedia.org)
  • require
  • Even with the sparse updates imposed by the oscillation, the Phased LSTM network achieves faster convergence than regular LSTMs on tasks which require learning of long sequences. (arxiv.org)
  • Study
  • The present study explored whether such context-dependence develops when sequence execution is fully memory-based-and thus no longer assisted by stimulus-response translations. (semanticscholar.org)
  • This study investigated whether negative priming can be observed in a sequence learning that is purely perceptual-based. (arvojournals.org)
  • approach
  • This paper introduces an approach to recommending the sequencing of e-learning modules for distance learners based on self-organisation theory. (ou.nl)
  • current
  • These forward-looking statements are based upon management's current expectations, are subject to known and unknown risks, and involve assumptions that may never materialize or may prove to be incorrect. (cnbc.com)
  • a substantial sample of individuals of the target species or other taxon is used, however many current studies are based on single individuals. (wikipedia.org)