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  • nucleotide
  • Second, misincorporation of nucleotides during DNA replication can yield mismatched basepairs, nucleotide insertions, and deletions ( M odrich 1991 ). (genetics.org)
  • c) detecting the chemically modified mismatched nucleotide using an immunochemical reagent under conditions wherein chemical modification of a homoduplex is not detected. (google.com)
  • Nevertheless, detection of single-base changes has improved rapidly with advances in single-nucleotide polymorphism (SNP) detection technologies ( K wok 2001 ), and this has fueled the application of new technologies to reverse-genetic mutational screening. (genetics.org)
  • duplexes
  • A point connecting a pair of homologous regions of DNA duplexes moves along the homology in a reaction intermediate of the homologous recombination. (genetics.org)
  • Early studies by Southern and others showed that when short single-stranded DNA probes are affixed to a solid surface, the efficiency with which they form duplexes with single-stranded DNA in free solution is sensitive to the presence of single-base-pair mismatches. (pnas.org)
  • Probes
  • Moreover, designing microarray probes with optimized sensitivity to mismatches should increase the accuracy of standard microarray applications such as copy-number variation detection and gene expression analysis. (pnas.org)
  • MUTYH
  • Right column''': The A:8-oxo-G base pairs can be recognized by MUTYH, which catalyzes the excision of the wrong A from opposite 8-oxo-G, leading to the formation of an AP site. (openwetware.org)
  • differences
  • This review focuses on these DNA mismatch-binding ligands, with an emphasis on their (often unusual) binding modes, as well as on the similarities and differences between the different classes of mismatch binders. (rsc.org)
  • Single
  • This single strand break is typically the substrate for DNA polymerase β (Pol β), which exerts DNA-deoxyribophosphodiesterase activity to cleave off the abasic sugar and uses the intact strand as template to synthesize the missing base ( 5 ). (pnas.org)
  • A general method for screening genomic or cDNA, or fragments and mixtures thereof, involves sample simplification by the generation of subsets and then subjecting the subsets to a modified mismatch scanning procedure that eliminates DNA having single stranded breaks after a MutSLH cleavage. (google.com)
  • methods
  • An imidazole-containing polyamide trimer, f-ImImIm, where f is a formamido group, was recently found using NMR methods to recognize T*G mismatched base pairs. (furman.edu)
  • The kinetics and thermodynamics for the polyamides binding to Watson-Crick and mismatched (containing one or two T*G, A*G or G*G mismatched base pairs) hairpin oligonucleotides were determined by surface plasmon resonance and circular dichroism (CD) methods. (furman.edu)