• Our findings reveal ErbB activation as a strong inductive signal for stem-cell proliferation. (nature.com)
  • Psoriasis lesions are characterized by hyper-proliferation with incomplete differentiation of epidermal keratinocytes and decreased keratinocyte apoptosis, associated with inflammatory cellular infiltrate in both dermis and epidermis [ 12 ]. (springer.com)
  • Proliferation of KRT14 -expressing basal cells leads to development of dermatoglyphics during the first trimester of gestation. (medscape.com)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
  • Bowen, 2004} detected keratinocyte proliferation by PCNA (proliferating cell nuclear antigen) marking, 13,9% of keratinocytes were stained, compared with 6,4% in normal skin. (globale-dermatologie.com)
  • We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)
  • We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
  • Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
  • Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
  • Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
  • It interferes with DNA synthesis by blocking methylation of deoxyuridylic acid and inhibiting thymidylate synthetase and, subsequently, cell proliferation. (medscape.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • According to the National Comprehensive Cancer Network (NCCN), the goal of treatment for basal cell carcinoma (BCC) is elimination of the tumor with maximal preservation of function and physical appearance. (medscape.com)
  • Diagnosis of TBL is challenging given its resemblance to basal cell carcinoma (BCC) but is important given possible malignant transformation and potential metastasis. (karger.com)
  • Foamy gland carcinoma of the prostate features cells with abundant finely vacuolated cytoplasm and pyknotic hyperchromatic nuclei. (medscape.com)
  • Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
  • ASPP2: A molecular switch of cancer initiating cells in Squamous Cell Carcinoma? (ukri.org)
  • Around 80% of all human cancers develop in the epithelium, and of the different epithelial cancer types, Squamous Cell Carcinoma (SCC) is the most deadly. (ukri.org)
  • Of the different tumour types arising in epithelial tissues, squamous cell carcinoma (SCC) is the most malignant and has the highest metastatic potential. (ukri.org)
  • No chromosome was found contrary to an extra chromosome 5 in the basal cell carcinoma. (globale-dermatologie.com)
  • In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)
  • Carcinoma of cervix is classified as per the WHO classification into primary tumors which are predominantly epithelial tumors, mesenchymal tumors and tumor like lesions, mixed epithelial stromal tumors, melanocytic, germ cell, and lymphoid tumors. (cytojournal.com)
  • Squamous cell carcinoma (SCC) in various morphological forms needs to be separated from other epithelial tumors for treatment modality selection. (cytojournal.com)
  • The most common chemotherapeutic agent used in superficial basal cell carcinoma is topical 5-fluorouracil. (medscape.com)
  • This agent is indicated for treatment of adults with metastatic basal cell carcinoma or with locally advanced basal cell carcinoma that has recurred following surgery, or those who are not candidates for surgery or radiation. (medscape.com)
  • It is not approved by the US Food and Drug Administration for treating basal cell carcinoma. (medscape.com)
  • Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
  • Basal cell carcinoma of the right lower lid. (medscape.com)
  • Br J mous cell carcinoma of the skin. (who.int)
  • The corneal epithelium is composed of stratified squamous epithelial cells on the outer surface of the eye, which acts as a protective barrier and is critical for clear and stable vision. (wjgnet.com)
  • NRG1Îą was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
  • Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
  • Thus, the aim of this study was to investigate if aerosolized nZnO exposure leads to changes in the cellular composition of the airway epithelium, using air-liquid interface (ALI)-differentiated primary human bronchial epithelial cells. (cdc.gov)
  • Methods: Normal human bronchial epithelial cells (NHBEpC) (at ≥ 90% viability) were seeded on 12-well porous 0.4 Âľm- cell culture inserts in basal cell culture medium. (cdc.gov)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • While Akt Ser 473 phosphorylation was readily detectable in AND-34 +/+ lens epithelial cells, it was markedly reduced in the AND-34 −/− lens epithelium. (molvis.org)
  • These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
  • Our preliminary results show that the tumour suppressor ASPP2 is able to repress p63 expression, is highly expressed in keratin 1-positive and keratin 14-negative stratified epithelial cells, and that differentiation induces ASPP2's expression in vitro. (ukri.org)
  • Due to changes in their epigenetic regulation, differentiated keratin 1-positive squamous epithelial cells may start to re-express keratin 14 and p63 to gain a proliferative potential. (ukri.org)
  • Androgen induced expression of androgen receptor (AR), and co-culture with rat urogenital sinus further induced expression of prostate-specific antigen, a hallmark of secretory cells, suggesting that E-PZ-iPS-like cells have the capacity to differentiate into prostatic basal and secretory epithelial cells. (nih.gov)
  • Our results suggest that iPS-like cells can be derived from prostatic epithelial cells. (nih.gov)
  • Later studies have demonstrated secretion of psoriasin from epithelial cells (5). (medicaljournals.se)
  • Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3-Par6-aPKC polarity complex. (rupress.org)
  • In epithelial cells, apical-basal polarity is maintained through the formation of several intercellular adhesion systems consisting of adherens junctions (AJs), desmosomes, and tight junctions (TJs). (rupress.org)
  • Hedgehog ligand-expressing cancerous epithelial cells that are activated by the hedgehog signaling pathway may cause growth-promotion. (medscape.com)
  • METHODS: The expression levels of SMYD5 and the mitochondrial transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator-1Îą (PGC-1Îą) were examined by Western blot, immunofluorescence staining, and immunohistochemistry in intestinal epithelial cells (IECs) and in colon tissues from human IBD patients and colitic mice. (bvsalud.org)
  • In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)
  • Neuregulin1Îą, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
  • The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • G ) Muzzle-infiltrating cells were isolated from LMC and Sox13 -/- mice and re-stimulated in vitro with PdBu/ionomycin to assess production of IL-5 and IL-13 by ILCs. (elifesciences.org)
  • D ) Muzzle-infiltrating cells were isolated from indicated mice, stimulated in vitro with PdBu/ionomycin, and analyzed for ιβ T cell subset-specific production of IL-17A and IL-22 and for CD4 + T cell production of IL-4, and IL-13. (elifesciences.org)
  • Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
  • E-PZ-iPS-like cells demonstrated pluripotency by differentiating into ectodermal, mesodermal, and endodermal cells in vitro, although lack of teratoma formation in vivo and incomplete demethylation of pluripotency genes suggested only partial reprogramming. (nih.gov)
  • Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models. (who.int)
  • Originally described by Headington in the 1970s, trichoblastoma (TBL) is now appreciated as a rare benign tumor that originates from the follicular germ cell line [1, 2]. (karger.com)
  • If prostate biopsies are performed following hormonal therapy for prostatic adenocarcinoma, the appearance of residual tumor cells can mimic xanthoma cells. (medscape.com)
  • Tumor cells usually retain prostatic epithelial antigenicity, and immunohistochemical stains can be used for their identification. (medscape.com)
  • Many of the current efforts regarding anti-cancer drug development are focused on directing tumor cells to undergo apoptosis. (mdpi.com)
  • Possible mechanisms for overcoming the resistance of HPV-infected tumor cells to anticancer drugs will be discussed. (mdpi.com)
  • We investigated whether a molecular classification of tumor cell differentiation, based on a developmental biology approach, can provide additional prognostic information. (nih.gov)
  • Our data indicate that BC can be subclassified into three subtypes, on the basis of their differentiation states: basal, intermediate, and differentiated, where only the most primitive tumor cell subpopulation within each subtype is capable of generating xenograft tumors and recapitulating downstream populations. (nih.gov)
  • We identify here three distinct BC subtypes on the basis of their differentiation states, each harboring a unique tumor-initiating population. (nih.gov)
  • It may increase tumor infiltration by lymphocytes, dendritic cells, and macrophages. (medscape.com)
  • The pathology of chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF) and most lung cancers involves the small airway epithelium (SAE), the single continuous layer of cells lining the airways ≥ 6th generations. (biomedcentral.com)
  • Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
  • Abnormal differentiation of the respiratory epithelium is a feature of idiopathic pulmonary fibrosis (IPF). (bmj.com)
  • The adult human airway epithelium is comprised of four major cell types, basal, club, goblet, and ciliated cells. (cdc.gov)
  • The presence of all four major cell types at protein level was confirmed by IF staining of the fully differentiated airway epithelium. (cdc.gov)
  • Since these cells reside at the bottom of the pseudostratified airway epithelium, it's possible that they were not directly impacted by nZnO exposure, at the doses/time points used. (cdc.gov)
  • Basal levels of p130Cas phosphorylation were higher in AND-34 +/+ than in AND-34 −/− lens epithelium. (molvis.org)
  • Alternatively, it is possible that SCC instead initially develops from the differentiated keratin 1-positive and keratin 14-negative suprabasal layer of the epithelium, which sits above the basal layer. (ukri.org)
  • Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)
  • Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1Îą and NRG1β. (bmj.com)
  • In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
  • The structure and structure-based modulation of ADAM17 are also described for better understanding of the various ADAM17 regulatory pathways in different cell types or tissues. (frontiersin.org)
  • These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
  • DNA methylation profiling identified key pathways and regulators of prostatic differentiation including Wnt5a, BPM7, PTEN, and NKx3.1 using E-PZ-iPS-lke cells. (nih.gov)
  • The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
  • In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
  • Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
  • This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
  • The aim of this study was to investigate the effect of endogenous psoriasin on disturbed keratinocyte differentiation in psoriasis. (medicaljournals.se)
  • Induction of keratinocyte differentiation caused concurrent expression of psoriasin and the differentiation marker involucrin. (medicaljournals.se)
  • The high expression level in psoriatic epidermis in conjunction with the disturbed differentiation process suggests that psoriasin is involved in keratinocyte differentiation. (medicaljournals.se)
  • Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)
  • It modulates keratinocyte differentiation. (medscape.com)
  • Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
  • Cells were harvested at 24 hours or 7 days post exposure, to investigate changes in (a) inflammation, (b) oxidative stress-related, and (c) cell-type specific markers using qPCR and/or IF staining. (cdc.gov)
  • Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)
  • Exposure to high dose of nZnO induced a significant decrease in expression of club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1) markers at mRNA levels. (cdc.gov)
  • How SCC develops is currently poorly understood, however, it is known that as well as expressing normal basal cell markers such as keratin 14, they also express markers normally found in differentiated squamous cells such as keratin 1. (ukri.org)
  • While it is possible that SCCs initially develop in basal cells, it is unclear why such basal cells in SCCs begin to express differentiation markers. (ukri.org)
  • Importantly, E-PZ-iPS-like cells re-expressed basal epithelial cell markers (CD44, p63, MAO-A) in response to prostate-specific medium in spheroid culture. (nih.gov)
  • Finally, when injected into mice, E-PZ-iPS-like cells expressed basal epithelial cell markers including CD44 and p63. (nih.gov)
  • The keratinocytes in active psoriatic lesions display an altered sequence and altered expression levels of the differentiation markers (2). (medicaljournals.se)
  • Led by Dr. Paula Sotomayor, researchers in the Department of Urologic Oncology at the Roswell Park Cancer Institute in Buffalo, New York have identified Oct4A in a small subset of prostate cells which were negative for many other types of more conventional cancer stem cells markers, such as markers of luminal epithelial and basal epithelial cell differentiation, including the markers ABCG2, NANOG, CD133 and AMACR. (cellmedicine.com)
  • Yet another subpopulation was found to co-express synaptophysin, while the majority of the Oct4A-expressing cells were found to co-express chromagranin A, both of which are neuroendocrine differentiation markers. (cellmedicine.com)
  • Further investigations will be focused especially on those various subpopulations of the cells that co-express the neuroendocrine differentiation markers chromagranin A and synaptophysin, both of which offer new and important pieces in the puzzle. (cellmedicine.com)
  • Exploiting large preexisting gene-expression databases, we developed a biologically supervised computational model to predict markers that correspond with BC differentiation. (nih.gov)
  • Cells expressing differential levels of epithelial and progenitor markers were isolated using flow cytometry sorting and characterised by qPCR and IF. (who.int)
  • During mitosis, cells undergo symmetrical cell division, while oocyte meiotic maturation undergoes two consecutive, asymmetric divisions that generate a totipotent haploid oocyte and two small polar bodies not involved in DNA replication. (bioone.org)
  • It is estimated that as much as 10% of the cell surface proteins undergo ectodomain shedding. (frontiersin.org)
  • In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
  • To establish the skin barrier and the epidermal structure, keratinocytes undergo a tightly regulated process of differentiation. (medicaljournals.se)
  • The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)
  • In parallel, the expression of basal cell marker(KRT5) was partially modulated by nZnO exposure. (cdc.gov)
  • We found that keratin 14 (KRT14) marks the most primitive differentiation state that precedes KRT5 and KRT20 expression. (nih.gov)
  • In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
  • They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
  • The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
  • Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. (thermofisher.com)
  • However, a major unresolved question is how each cell regulates the precise number of its motile cilia during differentiation. (elifesciences.org)
  • Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
  • Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
  • These E-PZ-iPS-like cells with normal karyotype gained expression of pluripotent genes typical of iPS cells (Tra-1-81, SSEA-3, Nanog, Sox2, and Oct4) and lost gene expression characteristic of basal prostatic epithelial cells (CK5, CK14, and p63). (nih.gov)
  • A further subpopulation of the cells expressing Oct4A were also found to co-express the embryonic stem cell marker Sox2, which is a transcription factor necessary for the self-renewal of undifferentiated cells. (cellmedicine.com)
  • Recent ChIP experiments of human and mouse embryonic stem cells have elucidated the architecture of the transcriptional regulatory circuitry responsible for cell determination, which involves the transcription factors OCT4, SOX2 and NANOG. (lu.se)
  • The switch stabilizes the expression levels of the three genes, and through their regulatory roles on the downstream target genes, leads to a binary decision: When OCT4, SOX2 and NANOG are expressed and the switch is ON, the self-renewal genes are ON and the differentiation genes are OFF. (lu.se)
  • Increasing the binding strength of NANOG to OCT4 and SOX2, or increasing its basal transcriptional rate, leads to an irreversible bistable switch: The switch remains ON even when the activating signal is removed. (lu.se)
  • The basal cells (BC) are the stem/progenitor cells of the SAE, responsible for the differentiation into intermediate cells and ciliated, club and mucous cells. (biomedcentral.com)
  • Airway progenitor cells contain two parental centrioles (PC) and form structures called deuterosomes that nucleate centrioles during amplification. (elifesciences.org)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • Three-dimensional cultures, adherent or suspension cells, bacteria, yeast, and even small parasites such as malaria can be queried. (genengnews.com)
  • Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
  • While abnormal keratin filament structure and function can explain hypohidrosis and epithelial differentiation abnormalities, the absence of dermatoglyphics is not well understood. (medscape.com)
  • The control of this transition from epithelial stem cell to differentiated corneocyte, which is abnormal in epidermal cancers, is not well understood. (stanford.edu)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • In addition to the low expression in normal skin and the high expression in the psoriatic epidermis, psoriasin is also expressed in conditions displaying abnormal differentiation. (medicaljournals.se)
  • Small airway basal cells were purified from brushed SAE of a healthy nonsmoker donor with a characteristic normal SAE transcriptome. (biomedcentral.com)
  • The resulting cell line (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. (biomedcentral.com)
  • Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
  • Any change in the numbers of these cell types is termed as 'airway remodeling', which is a disease-related phenotype in several lung diseases. (cdc.gov)
  • To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line. (biomedcentral.com)
  • The immortalized hSABCi-NS1.1 cell line has diverse differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and testing new pharmacologic agents. (biomedcentral.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • The research conducted by central investigators is quite broad, ranging from cell and molecular biology to tissue engineering, biophysics and nanobiology/medicine. (upenn.edu)
  • Although iPS cells have been generated from other terminally differentiated cells, the reprogramming of normal adult human basal prostatic epithelial (E-PZ) cells to a pluripotent state has not been reported. (nih.gov)
  • this finding indicates that the gene for NFJ syndrome is not located in the epidermal differentiation complex, the type II keratin cluster, or the desmosomal cadherin cluster, respectively. (medscape.com)
  • These observations support a mutation in a basal keratin gene as causing both blistering and pigmentary disorders. (medscape.com)
  • These observations challenge the current view that SCC is derived from basal cells (p63+, keratin 14+/keratin 1-), as ASPP2 is hardly expressed in this cell population. (ukri.org)
  • SCC cells may instead be derived from differentiated keratin 1-positive cells in the suprabasal layer. (ukri.org)
  • To determine the precise origin of SCC, ASPP2 will be deleted in keratin 14-positive basal cells and keratin 1-positive suprabasal cells using keratin 14-Cre or keratin 1-Cre respectively. (ukri.org)
  • In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. (rupress.org)
  • In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides. (upenn.edu)
  • Let us underline nevertheless that such cytokeratin expression is found in embryonic cell lines and that the study only concerned 10 specimens. (globale-dermatologie.com)
  • The stem cells within the basal layer, which contact the basement membrane, continually divide during the lifetime of the organism, providing a source of cells which progressively migrate upwards through the epidermis, differentiating and stratifying to form the barrier layer of the skin [ 11 , 14 ]. (springer.com)
  • Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membrane glycoprotein that in humans is encoded by the BCAM gene. (wikipedia.org)
  • ADAM17 is a metalloprotease and disintegrin that lodges in the plasmatic membrane of several cell types and is able to cleave a wide variety of cell surface proteins. (frontiersin.org)
  • A Disintegrin and Metalloproteases (ADAM), originally named metalloproteinases disintegrin cystein-rich (MDC), are membrane-anchored cell surface proteins containing both disintegrin and metalloproteinase domains. (frontiersin.org)
  • PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. (upenn.edu)
  • Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin-based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. (rupress.org)
  • The bulk RNA sequencing datasets ( GSE180823 ) and the single-cell RNA sequencing datasets ( GSE181306 ) are deposited in the Gene Expression Omnibus (GEO, NCBI) repository. (nature.com)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
  • Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
  • Coexisting foamy and nonfoamy adenocarcinoma with glandular differentiation devoid of basal cell lining is helpful in clarifying their malignant nature. (medscape.com)
  • Therefore, the authors concluded to a probable hyperplasic status in benign or malignant neoplasic cells. (globale-dermatologie.com)
  • Cells expressing Oct4A have been found in both benign and malignant prostate glands. (cellmedicine.com)
  • Its effect and effectiveness appear to be highly connected with the differentiation status of the cancerous cells, as Ang II appears to play a crucial role in the early and late stages of malignant transformation. (spandidos-publications.com)
  • however, direct antiproliferative effects against malignant cells and modulation of host immune response may be important. (medscape.com)
  • On the same day, the cells in the apical chamber were exposed to air (airlift) to create the ALI and allowed to differentiate for 28 days. (cdc.gov)
  • MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
  • Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
  • Lentivirus-mediated expression of SPDEF in hSABCi-NS1.1 cells induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. (biomedcentral.com)
  • As ErbB-family ligands and receptors are highly expressed within the stem-cell niche 7 , we hypothesize that strong endogenous regulators must control the pathway in the stem-cell compartment. (nature.com)
  • HYPOTHESIS: Do LRIG Proteins Regulate Stem Cell Quiescence by Promoting BMP Signaling? (nature.com)
  • Figure 3: Loss of Lrig1 causes crypt and stem-cell expansion. (nature.com)
  • Morrison, S. J. & Spradling, A. C. Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. (nature.com)
  • Jensen, K. B. & Watt, F. M. Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence. (nature.com)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
  • Advancing your pluripotent stem cell therapy research to clinical applications requires careful material selection as the quality of starting materials significantly impacts the properties of your final stem cell therapy product. (thermofisher.com)
  • At Thermo Fisher Scientific, we have developed Gibco Cell Therapy Systems (CTS) products across the pluripotent stem cell therapy workflow to ease the transition from discovery to commercial manufacturing. (thermofisher.com)
  • Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals. (thermofisher.com)
  • Hence, the stem cell can be manipulated to be self-renewing without the requirement of input signals. (lu.se)
  • Stem Cell Res Ther. (lu.se)
  • This effect was observed at both 24 hours and 7 days' time points, suggesting an early blockage in the cellular differentiation process, which requires further evaluation. (cdc.gov)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application. (bdbiosciences.com)
  • Especially in drug discovery, most such assays have been geared toward a particular reaction-whether it is recognition of a cell surface marker, triggering endocytosis, or exciting or inhibiting a phosphorylation cascade. (genengnews.com)
  • In embryonic stem cells and germ cells, properties of self-renewal and pluripotency are regulated by the Oct4A gene, a marker which has been suspected of playing a role in the origin of some cancers. (cellmedicine.com)
  • The zebrafish and Xenopus kidney are outlined in white dashed lines, and motile multiciliated cells in the kidney are pseudocolored in magenta. (xenbase.org)
  • f) Image showing basal body and axoneme of motile cilia (g) Image showing cross‐section and the 9 + 2 microtubule structure of motile cilia . (xenbase.org)
  • Multiciliated cells (MCC) contain hundreds of motile cilia used to propel fluid over their surface. (elifesciences.org)
  • Each MCC contains hundreds of motile cilia, microtubule-based organelles that generate the motive force to move fluid over the surface of the cell. (elifesciences.org)
  • Long-term Culture and Cloning of Primary Human Bronchial Basal Cells that Maintain Multipotent Differentiation Capacity and CFTR Channel Function. (academictree.org)
  • With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
  • Cancer stem cells are thought to be the source of some types of primary and recurrent cancers and are characterized by their high degree of potency and self-renewing capacity. (cellmedicine.com)
  • The lentivirus-mediated overexpression of psoriasin, mimicking the psoriatic milieu, gave rise to an altered regulation of differentiation genes and an expression pattern reminiscent of that in psoriatic epidermis. (medicaljournals.se)
  • Moreover, we examined alterations in the expression of genes related to cancer cell behaviour after Ang II treatment. (spandidos-publications.com)
  • In addition to regulating each other through feedback loops, these genes also regulate downstream target genes involved in the maintenance and differentiation of embryonic stem cells. (lu.se)
  • Fig. 6: Radiation exposure boosts Notch signaling and stemness in metastatic cells. (nature.com)
  • Metastatic-niche labelling reveals parenchymal cells with stem features. (nature.com)
  • Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. (techscience.com)
  • One explanation is that a selective advantage may be derived from their expression that enables basal cells to gain new properties. (ukri.org)
  • When co-injected with rat urogenital mesenchyme, E-PZ-iPS-like cells expressed AR and expression of p63 and CD44 was repressed. (nih.gov)
  • The differentiation-induced psoriasin expression was found to be mediated by the protein kinase C pathway. (medicaljournals.se)
  • The downregulation of psoriasin expression by small interfering RNA revealed that psoriasin mediates the expression of involucrin, desmoglein 1, transglutaminase 1 and CD24 in normal differentiation. (medicaljournals.se)
  • This pattern of expression suggests an involvement in epithelial differentiation. (medicaljournals.se)
  • Other patterns include arrangement of the histiocytes in cords or single cells that percolate between benign prostatic glands. (medscape.com)
  • These cells are pluripotent and capable of prostatic differentiation, and therefore provide a novel model for investigating epigenetic changes involved in prostate cell lineage specification. (nih.gov)
  • Cultures are subjected to a standard 2D viability assay to determine the health of the cells two days after treatment with the test compound. (genengnews.com)
  • When cultured on ALI, hSABCi-NS1.1 cells consistently formed tight junctions and differentiated into ciliated, club (SCGB1A1 + ), mucous (MUC5AC + , MUC5B + ), neuroendocrine (CHGA + ), ionocyte (FOXI1 + ) and surfactant protein positive cells (SFTPA + , SFTPB + , SFTPD + ), observations confirmed by RNAseq and TaqMan PCR. (biomedcentral.com)
  • To avoid elimination by this mechanism, human papillomaviruses (HPV) have developed several mechanisms that enable the cells they infect to elude both extrinsic and intrinsic apoptosis. (mdpi.com)
  • Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)
  • The aim of the present study was to evaluate the influence of angiotensin II (Ang II) on human EC cells. (spandidos-publications.com)
  • Biological assays were performed on well-[Ishikawa (ISH)] moderately (MFE-296) and poorly (MFE-280) differentiated human adenocarcinoma cancer cell lines, representing the G1, G2 and G3 stage of EC. (spandidos-publications.com)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • 1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
  • Chromatin immunoprecipitations in naive CD4, but not CD8, T cells, demonstrated association of the IL-4 promoter with acetylated histone. (doe.gov)
  • No benefit of addition of other biomarkers like p63 or ki67 is found in problem-solving in differentiation of HSIL from mimics or low-grade squamous intraepithelial lesion. (cytojournal.com)
  • Neural progenitors were derived by depleting feeder cells and culturing cells in suspension in neural differentiation basal medium (DFNB) for 2 days followed by 500nM all-trans retinoic acid treatment in DFNB. (nih.gov)
  • We exposed healthy mouse lung tissue to radiation before the induction of metastasis and observed a strong enhancement of cancer cell growth. (nature.com)
  • DR+ epidermal macrophage induction, and noma in young women: An evaluation of the Langerhans cell depletion. (who.int)
  • The ability of the host to trigger apoptosis in infected cells is perhaps the most powerful tool by which viruses can be cleared from the host organism. (mdpi.com)
  • In this manuscript, we review the current literature regarding how HPV-infected cells avoid apoptosis and the molecular mechanisms involved in these events. (mdpi.com)
  • Indeed apoptosis is inhibited in senescent cells. (globale-dermatologie.com)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
  • Nevertheless, with the discovery that cells expressing Oct4A are present in cancerous prostates in numbers that correlate with the severity of the malignancy, scientists are one step closer to understanding this particular form of cancer. (cellmedicine.com)
  • The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • As the authors describe in their publication, "rare cells that express Oct4 were identified in several somatic cancers, however, the differential contributions of the Oct4A and Oct4B variants were not determined. (cellmedicine.com)