SequencesGenesSubunitRRNABarcodesGenomeRevealsFishesMolecularMarkerPhylogenetic analysisBarcodeDelimitationLineageAnalysesVariationGapsApproachesConfirmsMicrobialBiodiversityNeotropicalWidelySpecies compositionStudyDiversityImplicationsConservationBirdsMethodsIdentificationIdentifyDataAnimalResultsAnalysisApproachComparisonProductsUseful tool
Sequences7
- DNA barcoding methods for fish are used to identify groups of fish based on DNA sequences within selected regions of a genome. (wikipedia.org)
- The female, male and immature larva were associated by mitochondrial COI sequences. (mapress.com)
- We examined the phylogenetic relationships and evolutionary history of the species and related congeners using partial COI gene sequences (DNA barcoding). (scienceopen.com)
- We sequenced the mitochondrial COI gene from 296 individuals from the 10 currently recognized species of billfishes, and combined these data with a further 57 sequences from previously published projects. (nova.edu)
- First developed by the Biologist Dr Paul D. N. Hebert, DNA barcoding uses DNA sequences as taxon 'barcodes' to identify species. (ncl.ac.uk)
- 2003. Molecular phylogeny of the stingless bees (Apidae, Apinae, Meliponini) inferred from mitochondrial 16S rDNA sequences. (ugm.ac.id)
- Species identification using DNA sequences, known as DNA barcoding has been widely used in many applied fields. (arborbiosci.com)
Genes13
- The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes. (ox.ac.uk)
- BackgroundGenetic markers like the nuclear ribosomal RNA (rRNA) genes, internal transcribed spacer regions, mitochondrial protein-coding genes, and genomes have been utilized for molecular identification of parasitic trematodes. (ox.ac.uk)
- This study presented novel and broadly applicable primers using the mitochondrial 12S and 16S rRNA genes for Digenea and aimed to show their suitability as alternative genetic markers for molecular identification of orders Plagiorchiida, Echinostomida, and Strigeida.ResultsOur results revealed that the mitochondrial 12S and 16S rRNA genes are suitable for trematode molecular identification, with sufficient resolution to discriminate closely related species and achieve accurate species identification through phylogenetic placements. (ox.ac.uk)
- The convenience and applicability of the newly designed primers and adequate genetic variation of the mitochondrial rRNA genes can be useful as complementary markers for trematode molecular-based studies.ConclusionsWe demonstrated that the mitochondrial rRNA genes could be alternative genetic markers robust for trematode molecular identification and potentially helpful for DNA barcoding where our primers can be widely applied across the major Digenea orders. (ox.ac.uk)
- Furthermore, the potential of the mitochondrial rRNA genes for molecular systematics can be explored, enhancing their appeal for trematode molecular-based studies. (ox.ac.uk)
- The novelty of utilizing the mitochondrial rRNA genes and the designed primers in this study can potentially open avenues for species identification, discovery, and systematics in the future. (ox.ac.uk)
- Luo A. R..,Zhang A. B..,Ho S. Y. W..,Xu W. J..,Zhang Y. Z..,...&Zhu C. D..(2011).Potential efficacy of mitochondrial genes for animal DNA barcoding: a case study using eutherian mammals. (ioz.ac.cn)
- More specifically, DNA barcoding involves sequencing a short fragment of mitochondrial genes that are then compared against a large database of pre-identified species, known as reference libraries. (uoguelph.ca)
- By analyzing two mitochondrial and two nuclear genes from three morphospecies of the European Lepidocyrtus lanuginosus species group (Collembola: Entomobryidae) from different geographic regions of Europe, this thesis focuses on exploring cryptic species / lineages diversity, their phylogeny, and the effects of historical geographic and climatic changes on the divergence and distribution of this species group in Europe. (uni-goettingen.de)
- One of the genes found in mitochondria, COI, is used for DNA barcoding. (minareport.com)
- This type of barcoding method does not always work when applied to species separated by short divergence times or that contain introgressed genes from closely related species. (arborbiosci.com)
- The present study analyzed the sequence data from four genes (Cytb, CO1, 16S and 12S rRNA) of mitochondrial genome for the attempt to estimate the relationships among five species such as Elops saurus, E. affinis, E. smithi, E. machnata and E. hawaiensis and to assess the phylogenetic utility of these markers. (notulaebiologicae.ro)
- The four mitochondrial marker genes used in this study showed different type of cluster and we could not confirm the relationship between the five Elops species. (notulaebiologicae.ro)
Subunit5
- DNA barcoding is a method promising fast and accurate identification of animal species based on the sequencing of the mitochondrial c oxidase subunit (COI) gene. (nova.edu)
- About 528 specimens of 111 chondrichthyan species and 34 families, collected from the Indian EEZ, were barcoded for a 655 bp region of the mitochondrial gene cytochrome c oxidase subunit 1 (COI). (sharkyear.com)
- Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. (environcj.in)
- The snails were identified using partial mitochondrial cytochrome c oxidase subunit one and nuclear internal transcribed spacer barcoding. (bvsalud.org)
- Current barcoding methods are usually based on a single mitochondrial locus, such as cytochrome c oxidase subunit I (COI). (arborbiosci.com)
RRNA1
Barcodes2
- We explored the use of naturally occurring somatic mutations in mitochondrial transcripts detected in single-cell RNA-seq as genetic lineage barcodes in HPSC. (biorxiv.org)
- In this study, we used an enrichment of scRNA-seq mitochondrial reads and a robust computational method to identify clonally relevant mitochondrial variants as endogenous genetic barcodes for clonal tracking of early embryonic hematopoiesis from hPSC. (biorxiv.org)
Genome3
- As a first step in evaluating the most variable molecular markers for further population genetic and conservation studies of this and other insects, the entire mitochondrial genome (mitogenome) was sequenced (16,094bp). (bioone.org)
- Li-Wei Wu , David C. Lees , and Shen-Horn Yen "The Complete Mitochondrial Genome of the Near-Threatened Swallowtail, Agehana maraho (Lepidoptera: Papilionidae): Evaluating Sequence Variability and Suitable Markers for Conservation Genetic Studies," Entomological News 121(3), 267-280, (1 June 2010). (bioone.org)
- The method is based on the amplification and sequencing of two short variable parts of the 16S region in the mitochondrial genome. (st-andrews.ac.uk)
Reveals3
- DNA barcoding of Chinese snakes reveals hidden diversity and conservation needs. (academictree.org)
- DNA barcoding reveals clear delineation between spawning sites for neritic versus oceanic fishes in the Gulf of Mexico. (actabiologicaturcica.com)
- Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. (environcj.in)
Fishes4
- We investigated statistical approaches to better characterize the DNA barcode gap through barcoding Canadian Pacific fishes, including Sockeye salmon," says Phillips. (uoguelph.ca)
- DNA Barcoding of Ichthyoplankton and Juvenile Fishes of a Tropical River in Malaysia. (actabiologicaturcica.com)
- DNA barcoding to fishes: current status and future directions. (actabiologicaturcica.com)
- We selected 500 independent nuclear markers for ray-finned fishes and designed a three-step pipeline for multilocus DNA barcoding. (arborbiosci.com)
Molecular4
- We discuss amplicon-based DNA barcoding and metabarcoding for the analysis of community diversity and molecular gut content analysis for assessing predator-prey relationships. (springer.com)
- In DNA Barcoding and Molecular Phylogeny (pp. 237-251). (actabiologicaturcica.com)
- 2009. Mitochondrial DNA as a marker of molecular diversity: a reappraisal. (environcj.in)
- DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. (environcj.in)
Marker5
- Master thesis in Biogeosciences: Design and evaluation of a new mitochondrial marker for Arcellinida (Amoebozoa) (Laboratory of soil Biology, Universities of Neuchâtel and Lausanne). (unine.ch)
- NAD9/NAD7 (mitochondrial nicotinamide adenine dinucleotide dehydrogenase gene)-A new "Holy Grail" phylogenetic and DNA-barcoding marker for Arcellinida (Amoebozoa)? (unine.ch)
- We also sequenced the rhodopsin gene from a subset of 72 individuals to allow comparison of mitochondrial results against a nuclear marker. (nova.edu)
- Our study highlights the power of mitochondrial variants as an endogenous marker for clonal tracking in modeling human development from stem cells. (biorxiv.org)
- Using an understudied taxon (Hymenoptera, Formicidae) found on a tropical island (Mauritius) where native flora and fauna have been threatened by 400 years of habitat modification and introduced species, we tested whether estimated incidences of diversity and complementarity were similar when measured by standard morphological alpha-taxonomy or phylogenetic diversity (PD) based on a standardized mitochondrial barcode and corroborating nuclear marker. (biomedcentral.com)
Phylogenetic analysis1
- Species delineation through barcoding analysis and Bayesian phylogenetic analysis, fully concur. (biomedcentral.com)
Barcode3
- However, prospective tracing approaches inevitably encounter outstanding challenges including barcoding precision, barcode diversity, and detection efficiency, which can skew inferred lineage relationships. (biorxiv.org)
- University of Guelph School of Computer Science professor Dr. Daniel Gillis , Department of Integrative Biology professor Dr. Robert Hanner and postdoctoral fellow Dr. Jarrett Phillips looked into DNA barcoding interpretation, focusing on the DNA barcode gap. (uoguelph.ca)
- Phillips J, Gillis D, Hanner R. Lack of Statistical Rigor in DNA Barcoding Likely Invalidates the Presence of a True Species' Barcode Gap . (uoguelph.ca)
Delimitation1
- Single-locus DNA barcoding and species delimitation of the sandfly subgenus Evandromyia (Aldamyia). (scienceopen.com)
Lineage2
- We modeled the development of embryonic tissues from hPSCs and delineated the ontogeny of hematopoietic cells by mitochondrial variant lineage tracing. (biorxiv.org)
- We further applied mitochondrial variant lineage tracing to spatial transcriptomics and identified the clonal development of both erythro-myeloid progenitors and their niche cells. (biorxiv.org)
Analyses2
- In recent years, large-scale community analyses have benefitted tremendously from advances in DNA barcoding technology. (springer.com)
- In the last two decades, DNA barcoding, the sequencing of short species-specific amplicons, has considerably simplified community analyses (Hebert et al. (springer.com)
Variation2
- The DNA barcoding gap asserts that the variation found across species is higher than the genetic variability seen within a species. (uoguelph.ca)
- However, the researchers actually found that 90 percent of the animal species have mitochondrial DNA variation that is similarly low. (minareport.com)
Gaps2
- However, the interpretation of DNA barcoding gaps often lacks statistical rigor. (uoguelph.ca)
- ABGD delimitated species barcoding gaps with K2P distances of 0.055-0.095 and 0.06-0.115 for COI and COII, respectively, within the species group. (uni-goettingen.de)
Approaches1
- Barcoding and metabarcoding approaches yield rigorous and extensive data on recruitment, ecology and geographic ranges of fisheries resources. (wikipedia.org)
Confirms1
- The present study confirms the ability of DNA barcoding for the accurate identification of sharks, rays, and their products from Indian waters. (sharkyear.com)
Microbial2
- Environmental barcoding studies are showing that microbial Eukaryotes represent a large part of the world diversity. (unine.ch)
- DNA barcoding to map the microbial communities: Current advances and future directions. (actabiologicaturcica.com)
Biodiversity2
- The world's largest DNA barcoding centre is the Centre for Biodiversity Genomics at the University of Guelph. (uoguelph.ca)
- DNA barcoding provides a reliable means of identifying both known and undiscovered biodiversity within the closely related taxa of the Albitarsis Group. (biomedcentral.com)
Neotropical1
- DNA barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco. (actabiologicaturcica.com)
Widely1
- DNA barcoding is widely used for specie cataloging for unambiguous identification and conservation. (environcj.in)
Species composition2
- eDNA samples and barcoding methods are used in water management, as species composition can be used as an indicator of ecosystem health. (wikipedia.org)
- Species composition and diversity of fish larvae in the Subtropical Convergence Zone of the Sargasso Sea from morphology and DNA barcoding. (actabiologicaturcica.com)
Study5
- In this study, we explore the prospects for DNA barcoding in one particular fish group, the billfishes (suborder Xiphioidei-swordfish, marlins, spearfishes, and sailfish). (nova.edu)
- Interdisciplinary research team enhances DNA barcoding statistical rigor in new research study. (uoguelph.ca)
- The study also aims to contribute to the regional mitochondrial cytochrome oxidase I information pool, to support other potential uses. (nerc.ac.uk)
- In this study, the accuracy of morphological identification of fish eggs and larvae was investigated using DNA barcoding. (actabiologicaturcica.com)
- Current study may contribute towards the development of a reference DNA barcoding database of West African birds. (environcj.in)
Diversity2
- In recent years, DNA barcoding methods, which are used to identify species diversity in the Tree of Life, have been also applied to botanicals and plant-derived dietary supplements. (thieme-connect.com)
- We anticipate that, as the cost of DNA sequencing continues to fall that our multilocus barcoding approach will eclipse existing single-locus DNA barcoding methods as a means to better understand the diversity of the living world. (arborbiosci.com)
Implications1
- Patterns of evolution of mitochondrial ytochrome c oxidase I and II DNA and implications for DNA barcoding. (environcj.in)
Conservation1
- With reference libraries growing exponentially, there is a need to develop meaningful ways to analyze the vast amounts of available DNA barcoding information, which can then be applied in fields ranging from forensics to resource conservation. (uoguelph.ca)
Birds1
- DNA Barcoding Korean Birds. (environcj.in)
Methods5
- Barcoding methods can also be used for biomonitoring and food safety validation, animal diet assessment, assessment of food webs and species distribution, and for detection of invasive species. (wikipedia.org)
- In fish research, barcoding can be used as an alternative to traditional sampling methods. (wikipedia.org)
- Barcoding methods can often provide information without damage to the studied animal. (wikipedia.org)
- Barcoding and metabarcoding methods are particularly useful in studying endangered or elusive fish, as species can be detected without catching or harming the animals. (wikipedia.org)
- Three methods based on mitochondrial COI and COII congruently identified ten and nine distinct genetic lineages in the morphospecies L. cyaneus and L. lanuginosus, respectively. (uni-goettingen.de)
Identification4
- For example, barcoding and metabarcoding can help identifying fish eggs to species to ensure reliable data for stock assessment, as it has proven more reliable than identification via phenotypic characters. (wikipedia.org)
- Species identification cannot be made with only DNA barcoding method in ichthyoplankton studies. (actabiologicaturcica.com)
- DNA barcoding was successfully used for the accurate identification of chondrichthyans in the Indian commercial marine fishery. (sharkyear.com)
- 2007. Validation of the barcoding gene COI for use in forensic genetic species identification. (environcj.in)
Identify2
- DNA barcoding promised to identify the genetic "signatures" of global species in a time of extinction and ongoing environmental crisis. (uoguelph.ca)
- Both of our empirical and simulated results demonstrated that under limited gene flow and enough separation time, we could correctly identify species using multilocus barcoding method. (arborbiosci.com)
Data1
- The interspecies K2P genetic distance was 0.0158 and intraspecies distance was 0.0042 based on the barcoding gene, CO1 sequence data. (notulaebiologicae.ro)
Animal2
- After looking at the mitochondrial DNA of thousands of animal species, including humans, researchers reached the stunning conclusion that nearly every species dates back just 100,000 to 200,000 years. (minareport.com)
- According to their findings, most - if not all - animal species actually got their start 200,000 years ago with their mitochondrial clock essentially set at zero. (minareport.com)
Results1
- However, morphological identifications should be supported with DNA barcoding using an integrative approach to minimize the error rate and obtain more accurate results. (actabiologicaturcica.com)
Analysis1
Approach2
- Firstly, we used a citizen science approach in collaboration with municipal water providers who sampled groundwater organisms in their spring catchment boxes over multiple weeks, followed by DNA barcoding. (eawag.ch)
- adelum, where the COI barcoding approach failed. (arborbiosci.com)
Comparison1
- While nuclear DNA differs dramatically from species to species, animals have the same mitochondrial DNA, making a good basis for comparison. (minareport.com)
Products1
- Barcoding can be used to validate the labelling of products and to trace their origin. (wikipedia.org)
Useful tool2
- DNA barcoding has been proposed as a highly useful tool for species recognition, although its discriminatory utility has not been verified in closely related taxa across a wide geographic distribution. (biomedcentral.com)
- DNA barcoding as a useful tool for identifying non-native species of freshwater ichthyoplankton in the neotropics. (actabiologicaturcica.com)