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  • secrete
  • Based on these results, we conclude that α-GalCer inhibits HBV replication by directly activating NKT cells and by secondarily activating NK cells to secrete antiviral cytokines in the liver. (rupress.org)
  • Antigen
  • High affinity monoclonal antibodies to hepatitis B surface antigen (HBsAg) produced by somatic cell hybrids. (naver.com)
  • Hematopoietic antigen receptors such as the TCR, the B cell receptor, or Fc receptors are generally composed of at least two different subunits, one representing the ligand binding site, the other being responsible for signal transduction after receptor engagement. (rupress.org)
  • After binding of an external ligand (e.g., antigen), ITAMs become phosphorylated by protein tyrosine kinases of the src family such as Lck, Fyn, and Lyn, and this initial phosphorylation event then allows propagation of the signal from the cell surface into the cytoplasmic compartment ( 1 ). (rupress.org)
  • In the case of the TCR, the ligand binding site is constructed from the polymorphic α- and β chains, which form a disulfide-linked heterodimer that interacts with peptide/MHC complexes presented on the surface of antigen presenting cells ( 2 )( 3 )( 4 ). (rupress.org)
  • Indeed, DCs deficient in MARCH1 or MHCII ubiquitination both failed to generate antigen-specific T reg cells in vivo and in vitro, although both exhibited an increased capacity for antigen presentation in parallel with the increased surface MHCII. (rupress.org)
  • DNA vaccination is a technique for protecting against disease by injection with genetically engineered DNA so cells directly produce an antigen, producing a protective immunological response. (wikipedia.org)
  • Reactivity
  • 5. The antibody of claim 1 wherein the antibody demonstrates positive reactivity with phosphotyrosine-containing proteins from animal cells. (google.ca)
  • 11. The cell line of claim 7 wherein the antibodies demonstrate positive reactivity with phosphotyrosine-containing proteins from animal cells. (google.ca)
  • viral
  • The CXB strain distribution patterns of detected Ags did not match the distribution pattern of the BALB/c MTV6 viral genome indicating that none of the detected Ags were known viral superantigens. (mysciencework.com)
  • The possibility that NKT cells play a role in the control of viral infections has not been carefully examined. (rupress.org)
  • Based on the abundance of NKT cells in the liver and their ability to produce antiviral cytokines, it is possible that activated NKT cells might inhibit viral replication during HBV infection. (rupress.org)
  • Mouse
  • Olsen, A.S., O.W. McBride, and D.E. Moore (1981) Number and size of human X chromosome fragments transferred to mouse cells by chromosome-mediated gene transfer. (springer.com)
  • Chopan, M. and L. Kopelovich (1981) The suppression of tumor-igenicity in human X mouse cell hybrids. (springer.com)
  • It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. (biologists.org)
  • The suitability of BALB/c embryos for maximising the ES cell contribution to mouse ES cell chimaeras is also discussed. (biologists.org)
  • Are mouse lens epithelial cells more sensitive to γ-irradiation than lymphocytes? (biomedsearch.com)
  • In this pilot study we compared for the first time the radiation sensitivity of mouse lens epithelial cells (LECs) and mouse lymphocytes. (biomedsearch.com)
  • After the interaction of the α-GalCer-CD1d complex with the Vα14/Vβ8 TCR, intrahepatic mouse NKT cells become activated ( 12 )( 13 )( 14 ). (rupress.org)
  • The Ly-49 family of inhibitory receptors plays a major role in regulating mouse NK cell cytotoxicity. (jimmunol.org)
  • This research focuses on four discrete neuron types in the mouse CNS that are amenable to accurate and economical quantitative analysis: retinal ganglion cells (large projection neurons), photoreceptors, horizontal cells (smaller interneurons), and neurons in the dorsal lateral geniculate nucleus. (nervenet.org)
  • We will study four well-defined and interconnected cell populations in the mouse primary visual system (photoreceptors, horizontal cells, retinal ganglion cells, and neurons in the dorsal lateral geniculate nucleus). (nervenet.org)
  • 7. The medium according to claim 6, wherein said cells to be cultured are obtained from a B6C3Fe-a/a-op/op mouse strain. (google.com)
  • identical
  • Minor histocompatibility (H) Ags elicit T cell responses and thereby cause chronic graft rejection and graft-vs-host disease among MHC identical individuals. (jimmunol.org)
  • hybridization
  • The occurrence of complementation within or between the species barrier, following cell hybridization or cocultivation, indicates the recessive nature of the corresponding mutations and the possible homology of the relevant genetic systems in different mammalian species. (springer.com)
  • myeloid cells
  • Tumor-associated CD8+ T cell tolerance induced by bone marrow-derived immature myeloid cells. (nih.gov)
  • In this study, we demonstrate that STAT6 in bone marrow-derived myeloid cells was sufficient for the production of CCL17, CCL22, CCL11, and CCL24 and for Th2 lymphocyte and eosinophil recruitment into the allergic airway. (jimmunol.org)
  • Selective depletion of CD11b + myeloid cells in the lung identified these cells as the critical cellular source for the chemokines CCL17 and CCL22. (jimmunol.org)
  • These data reveal that CD11b + myeloid cells in the lung help orchestrate the adaptive immune response in asthma, in part, through the production of STAT6-inducible chemokines and the recruitment of Th2 lymphocytes into the airway. (jimmunol.org)
  • terminally differentiated
  • The entry of lymphoid progenitors into the B cell lineage and the subsequent developmental progression to terminally differentiated plasma cells is controlled by a multitude of transcriptional regulators ( 3 , 6 ). (jimmunol.org)
  • recognize
  • According to the missing-self hypothesis, NK cells recognize and eliminate cells that fail to express self-MHC molecules ( 5 ). (jimmunol.org)
  • Among them, Ly-49A has been shown to recognize H-2D d and D k molecules and inhibit NK cytotoxicity against target cells expressing those class I MHC molecules ( 2 ), and Ly-49C is an inhibitory receptor with much broader specificity for class I MHC, including those of H-2 d,b,k and H-2 s haplotypes ( 3 , 4 ). (jimmunol.org)
  • stimulate
  • This rapid elimination limited the ability of allogeneic DCs to stimulate primary and recall T cell responses. (jimmunol.org)
  • involves
  • The direct pathway involves T cells directly responding to intact, foreign MHC molecules expressed by donor-derived DCs. (jimmunol.org)
  • human
  • P. A. Jones, J. S. Rhim, and H. Isaacs, The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells. (springer.com)
  • L. Larizza, G. Simoni, M. Stefanini, and L. De Carli, Spontaneous and Griseofulvin induced segregation for 8-Azaguanine resistance in hybrids from a human heteroploid line. (springer.com)
  • E. J. Stanbridge, and J. Wilkinson, Analysis of malignancy in human cells: malignant and transformed phenotypes are under separate genetic control, Proc. (springer.com)
  • Larizza L., Tenchini M.L., Rampoldi E., De Carli L., Colombi M., Barlati S. (1983) Derepression and Modulation of Plasminogen Activator in Human Cell Hybrids and Their Segregants. (springer.com)
  • The high SCE mutants found among rodent cells (as those seen in unstable rodent-human hybrid cells) are more likely the result of chromosomal loss or rearrangement occurring in culture at one or more of the genetic systems hypothesized above. (springer.com)
  • Klebe, R.J., T. Chen, and F.H. Ruddle (1971) Mapping of a human genetic regulator element by somatic cell genetic analysis. (springer.com)
  • Szybalski, W., and E. Szybalska (1962) Drug sensitivity as a genetic marker for human cell lines. (springer.com)
  • assays
  • Infectivity assays with several cell lines showed that glyco-gag increases XMRV infectivity in all cell lines tested, but the level of this increase varies in different cell lines. (biomedcentral.com)
  • Growth
  • However, for spatial analysis of clonal growth and cell mixing, it would be more advantageous to control the variation and produce mainly chimaeras with only a small proportion of cells that carry a lineage marker. (biologists.org)
  • production
  • In contrast, STAT6 in airway-lining cells did not mediate chemokine production or support cellular recruitment. (jimmunol.org)
  • The Isolation of rodent clonal cell lines with a stable high rate of SCEs and the production of somatic cell hybrids between them and BS cells offer a promising experimental tool for studying the biology of SCEs in general and the genetics of BS in particular. (springer.com)
  • 3. Determine whether the variation in neuron number is due to differences in cell production or cell death. (nervenet.org)
  • Thus, MARCH1-mediated MHCII ubiquitination in DCs is required for proper production of naturally occurring T reg cells, suggesting a role in balancing immunogenic and regulatory T cell development. (rupress.org)
  • peptide
  • These observations indicate that at least one class II-restricted minor H Ag is recognized by Th cells as a peptide bound to class II molecules. (mysciencework.com)
  • Activation
  • Both of these events were temporally associated with the rapid disappearance of NKT cells from the liver, presumably reflecting activation-induced cell death, and by the recruitment of activated NK cells into the organ. (rupress.org)
  • In addition, the data suggest that therapeutic activation of NKT cells may represent a new strategy for the treatment of chronic HBV infection. (rupress.org)
  • These studies suggest that MARCH1 plays a regulatory role in T cell activation during immune responses. (rupress.org)
  • line
  • 9. The cell line of claim 7 wherein the antibodies each have a molecular weight of about 150,000 daltons and are classified as γ 1 K antibodies. (google.ca)
  • 13. An antibody-producing cell line having the identifying characteristics of ATCC HB 8190. (google.ca)
  • To determine whether Th hybrids recognized peptides bound to I-Ab, I-Ab molecules were immunoprecipitated from the LB27.4 cell line that stimulated the A6.5.5 hybrid (HTT-4 specific). (mysciencework.com)
  • helper
  • Live, attenuated vaccines, such as smallpox and polio vaccines, are able to induce killer T-cell (TC or CTL) responses, helper T-cell (TH) responses and antibody immunity. (wikipedia.org)