Prophage-encoded phage resistance systems such as abortive infection system (Abi), confer to the host resistance to phage infection, thus increasing its overall fitness. (ucc.ie)
Among the different systems that exist in lactococci, abortive infection is highly significant because it is the cell's strongest defense against the phages that most often disrupt cheese making. (ncsu.edu)
This review addresses abortive infection in lactococci relative to similar phenomena in other bacteria. (ncsu.edu)
cremoris MG1363 that conferred phage resistance to the integrants. (omicsdi.org)
Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. (omicsdi.org)
This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. (omicsdi.org)
Buttermilk2
Lactococcus lactis is a gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
Lactococcus lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. (wikipedia.org)
Lactobacillus1
Site-specific integration in Lactobacillus acidophilus ADH: Cloning and sequence analysis of the attachment site of the bacteriophage phiadh. (ncsu.edu)
Plasmid3
The cryptic plasmids from this strain were coelectroporated, along with the shuttle vector pSA3, into the plasmid-free host L. lactis LM0230. (omicsdi.org)
In addition to pSA3, erythromycin- and phage-resistant isolates carried pSRQ900, an 11-kb plasmid from L. lactis W-37. (omicsdi.org)
Characteristics of phage abortion conferred in lactococci by the conjugal plasmid pTR2030. (ncsu.edu)
Morphology2
It is suggested that the L. lactis supernatant mainly affects Salmonella motility through disruption of flagellar rotation rather than through irreversible damage to morphology and physiology. (wikipedia.org)
Lactococcus lactis In terms of cell morphology, lactococci have spherical or ovoid-shaped cells and occur singly or in chains (Figure 1). (locke-movie.com)
2021. Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. (ulaval.ca)
AbiU confers resistance to phages from the three main industrially relevant lactococcal phage species: c2, 936, and P335. (omicsdi.org)
Evidence indicates that AbiU1 is responsible for phage resistance and that AbiU2 may downregulate phage resistance against 936 and P335 type phages but not c2 type phage. (omicsdi.org)
pSRQ900 did not confer any resistance to phages of the P335 species. (omicsdi.org)
This Abi system exhibits activity against phages infecting Lb. brevis and Lactococcus lactis strains. (ucc.ie)
The presence of such prophage-encoded systems highlights the importance of temperate phages for Lb. brevis strains and, in some cases, may explain their resistance to phage infection. (ucc.ie)
Phenotype1
The orientation and location of the insertions suggested that the phage resistance phenotype was caused by a chromosomal gene turned on by a promoter from the inserted construct. (omicsdi.org)
Phage infection2
Lactococcus lactis W-37 is highly resistant to phage infection. (omicsdi.org)
ABORTIVE PHAGE INFECTION AND RESTRICTION MODIFICATION ACTIVITIES DIRECTED BY PTR2030 DETERMINANTS ARE ENHANCED BY RECOMBINATION WITH CONJUGAL ELEMENTS IN LACTOCOCCI. (ncsu.edu)
Bacteria5
L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
Lactose fermentation In one study that sought to prove that some fermentation produced by L. lactis can hinder motility in pathogenic bacteria, the motilities of Pseudomonas, Vibrio, and Leptospira strains were severely disrupted by lactose utilization on the part of L. lactis. (wikipedia.org)
Detection of nucleotide modifications in bacteria and bacteriophages: Strengths and limitations of current technologies and software. (ulaval.ca)
This paper is a review of MDR (Multiple rug Resistance) related issues which have become a global problem and on the possible role of bacteriocins as an effective option for fighting against MDR disease causing bacteria. (bbrc.in)
Antibiotic resistance1
The phenomena of antibiotic resistance can be attributed to inappropriate, self medication and excessive prescription of antibiotics over the past several years all over the world according to Carlet et al (2012). (bbrc.in)
Genes1
Consistent with the results from comparative genomics (see references above), this resulted in L. lactis losing or downregulating genes that are dispensable in milk and the upregulation of peptide transport. (wikipedia.org)
Dairy4
The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. (wikipedia.org)
L. lactis is mainly isolated from either the dairy environment, or plant material. (wikipedia.org)
The use of L. lactis in dairy factories is not without issues. (wikipedia.org)
Abstract The longevity of mesophilic lactococci in dairy fermentations depends to a large extent on whether or not the strains carry effective phage-resistance mechanisms. (ncsu.edu)
Bacteriocin1
Bonhi K. L. R, Imran S. Role of Bacteriocin in Tackling the Global Problem of Multi-Drug Resistance : An Updated Review. (bbrc.in)
Bacterial1
Bacterial resistance to antimicrobials has reached an unacceptable level which threatens the very existence of man and animal alike if the situation is not corrected in the near future. (bbrc.in)
Kenya1
Gender-Responsive Design of Bacteriophage Products to Enhance Adoption by Chicken Keepers in Kenya. (ulaval.ca)
Characteristics1
Lactococcus lactis has been demonstrated to be a promising candidate for the delivery of functional proteins because of its noninvasive and nonpathogenic characteristics. (wikipedia.org)
Produce2
L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). (wikipedia.org)
lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. (wikipedia.org)
Expression1
Alternative expression systems including L. lactis and an acellular production system were evaluated. (europa.eu)
Aside from its high use in industrial application, Lactococcus lactis can also be found in the wild on plants and within the digestive tract of cows. (locke-movie.com)
Early1
2022. Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution. (ulaval.ca)