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Lactococcus lactisBacteriocinsLactococcusNisinLactobacillusCheeseMolecular Sequence DataDNA, BacterialFermentationPlasmidsBacteriophagesGenes, BacterialBacterial ProteinsBase SequenceAmino Acid SequenceLactobacillaceaeGene Expression Regulation, BacterialAcetoinFood MicrobiologyConjugation, GeneticCloning, MolecularTransformation, BacterialCulture MediaGram-Positive Asporogenous RodsLactobacillus plantarumCultured Milk ProductsMilkAntibiosisStreptococcus mutansOperonStreptococcusDiacetylMitomycinsSequence Analysis, DNAOpen Reading FramesMeat ProductsRestriction MappingLactoseEnterococcus faeciumChromosomes, BacterialLactobacillus acidophilusPediococcusHydrogen-Ion ConcentrationSodium NitriteLeuconostocDNA Restriction-Modification EnzymesDNA Transposable ElementsCaseinsFood PreservationTemperatureSequence Homology, Amino AcidAmino Acids, Branched-ChainDeoxyribonucleases, Type II Site-SpecificMutagenesis, InsertionalEscherichia coliSiphoviridaeAminopeptidasesDrug Resistance, MicrobialGenome, BacterialSpecies SpecificityMycobacterium avium subsp. paratuberculosisMultigene FamilyEndopeptidasesMutationCell WallBlotting, SouthernExtrachromosomal InheritanceDairy ProductsGenetic Complementation TestLysogenyRNA, BacterialSequence Homology, Nucleic AcidKluyveromycesLactic AcidBacteriological TechniquesNucleic Acid HybridizationEnterococcus faecalisSequence AlignmentTranscription, GeneticSodium ChloridePolymerase Chain ReactionPropionibacteriumStreptococcus thermophilusSubstrate SpecificityPhenotypeListeriaKinetics
LactobacillusStreptococcusNisinEnterococcusStaphylococcusParacaseiPediococcusBacillusGarvieaeProbiotic cultureRibosomally synthesizedHydrogen peroxideOrganic acidsForamStrainsPathogenic bacteriaAntimicrobial peptidesMicroorganismsInhibitoryCharacterizationPeptidesBacterialPathogensAntibioticsYogurtIncubationTherapeuticMilkNaturalGRASMethodsLactic acidProducePharmaceuticalRoleEnzymesHeatCreate
Lactobacillus9
Streptococcus4
  • Streptococcus thermophilus formerly known as Streptococcus salivarius subsp. (wikipedia.org)
  • Streptococcus salivarius subsp. (wikipedia.org)
  • As contagens de bactérias láticas do queijo de coalho dos produtores A e B foram na ordem de 106 e as maiores contagens (109 UFC/g) foram observadas no queijo do produtor C. Foram selecionadas 49 bactérias láticas típicas das três propriedades e os gêneros predominantes foram: Enterococcus, Lactococcus, Streptococcus e Leuconostoc. (bvsalud.org)
  • The lactic acid bacteria counts of coalho cheese from producers A and B were 106 and the highest counts (109 UFC/g) were found in cheese samples from producer C. Forty-nine lactic acid bacteria from three rural properties were selected and predominant genera was Enterococcus, Lactococcus, Streptococcus and Leuconostoc. (bvsalud.org)
Nisin5
  • Nisin is the only bacteriocin approved by US-FDA for commercial application in food products. (researchsquare.com)
  • The green consumerism is the basis for the development of alternative approaches for food preservation, like the use of natural compounds (essential oils, lysozyme, nisin and other bacteriocins, chitosan) and nonthermal treatments (high hydrostatic pressures, homogenization, microwave, irradiation). (eurekaselect.com)
  • Nisin is a natural antimicrobial peptide used as food preservative produced by Lactococcus lactis , that inhibits the outgrowth of spores, the growth of a variety of Gram-positive and Gram-negative bacteria. (usp.br)
  • This study aims to evaluate growth conditions for L. lactis as well as the effect in nisin production when utilizing milk whey and skimmed milk. (usp.br)
  • Lactococcus lactis ATCC 11454 was developed in a rotatory shaker (30°C/36 h/100 rpm) in diluted skimmed milk and nisin expression, growth parameters and media components were also studied. (usp.br)
Enterococcus2
Staphylococcus1
Paracasei1
  • Therefore, in the present study, a new human skin-derived Lactococcus paracasei strain, HS-05, was isolated, and its morphological characteristics were investigated. (springeropen.com)
Pediococcus2
Bacillus2
  • This work aimed to investigate the effect of dietary Lactococcus lactis and Bacillus subtilis on the immune responses, intestinal microbiota, and resistance to pathogens of Oscar, Astronotus ocellatus . (ij-aquaticbiology.com)
  • Investigating antibiotic activity of the genus Bacillus strains and properties of their bacteriocins in order to develop next-generation pharmaceuticals. (jfrm.ru)
Garvieae2
  • Studies describing antagonistic activity and disease resistance efficacy of potential probiotics towards lactococcosis caused by Lactococcus garvieae, Lactococcus lactis, Lactococcus piscium, and Lactococcus raffinolactis are limited. (ac.ir)
  • Fish lactococcosis, particularly caused by Lactococcus (L.) garvieae, has become a serious recurring bacterial disease in farmed fish, and due to the disease pathogenesis, its treatment is often temporary and, in some cases, ineffective. (ac.ir)
Probiotic culture2
  • The study provides information about broad spectrum bacteriocin in native probiotic culture and paves a way towards its application in functional foods as biopreservative agents. (researchsquare.com)
  • Pathogens' inhibition was mostly mediated by the production of organic acids, but neutralization experiments strongly suggested the presence of additional antimicrobial compounds in probiotic culture supernatants such as the bacteriocin Lantibiotic B, whose gene was detected in the genome of B. longum KABP042. (frontiersin.org)
Ribosomally synthesized2
  • Bacteriocins are ribosomally synthesized proteinaceous compounds produced by bacteria to protect themselves as well as to competitively colonize in the gut through elimination of undesirable organisms. (researchsquare.com)
  • The bacteriocins are very small ribosomally synthesized peptide molecules secreted by archea, gram-positive and some of gram-negative bacteria (Klaenhammer et al. (bbrc.in)
Hydrogen peroxide1
  • LAB that are usually designated as generally recognized as safe (GRAS) status in foods can also exert a biopreservative effect against other microorganisms as a result of competition for nutrients and/or of the production of antagonistic compounds such as organic acids, diacetyl, acetoin, hydrogen peroxide, antibiotics, and bacteriocins (Schillinger et al. (e-fas.org)
Organic acids1
  • The antimicrobial activity of LAB is mainly based on the production of metabolites such as organic acids, hydroperoxide and bacteriocins [ 14 ]. (springeropen.com)
Foram2
  • Para tanto as células de L. lactis foram desenvolvidas em agitador rotacional (30°C/36 h/100 rpm) e a atividade de nisina, os parâmetros de crescimento e os componentes do meio de cultivo foram analisados. (usp.br)
  • Dos 20 isolados, 19 apresentaram halos de inibição sobre as três bactérias patogênicas, com zonas de inibição de 2 a15 mm, e os maiores halos foram formados por Lactococcus lactis ssp lactis sobre S. enterica e S. aureus. (bvsalud.org)
Strains8
  • In the experiment, two different strains of bacteria are used to make reduced-fat cheddar cheese: a strain of Lactococcus lactis and a strain of S. thermophilus. (wikipedia.org)
  • It was concluded that applying both L. lactis and S. thermophilus strains would create higher-quality reduced-fat cheese with characteristics like those of regular cheese. (wikipedia.org)
  • Meanwhile, these bacteriocins produced by the tested LAB strains also exhibited histamine-degradation ability. (e-fas.org)
  • Dobson et al 2012 reviewed the role of antimicrobial peptides, or bacteriocins, produced by bacteria in establishing competitive exclusion of pathogenic strains. (drvitaminsolutions.com)
  • Mobility was only observed in the strains Lactococcus lactis subsp. (jfrm.ru)
  • Lactiplantibacillus plantarum D13 shows antistaphylococcal and antilisterial activity, probably due to the synthesis of a presumptive bacteriocin with antibiofilm capacity released in the cell-free supernatant (CFS), whose inhibitory effect is enhanced by cocultivation with susceptible strains. (bvsalud.org)
  • The purpose of the research paper was firstly to identify bacteriocin-producing lactic acid bacteria characterizing strains with anti-listeria activity and, secondly, to characterize bacteriocin evaluating its in vitro efficiency as a natural preservative and, thirdly, to evaluate the anti-listeria effect of the bacteriocinogenic strain of Lactiplantibacillus plantarum in cheeses and produce an edible film with anti-listerial effect. (bvsalud.org)
  • Of 355 lactic acid bacteria strains tested, two were able to produce bacteriocin against Listeria monocytogenes and were identified as Lactiplantibacillus plantarum and Lactiplantibacillus pentosus. (bvsalud.org)
Pathogenic bacteria2
  • Bacteriocins are powerful bactericidal peptides produced and secreted by a varied group of micro- organisms including yeast, protozoa and of course bacteria and they cause death and removal of non bacteriocin producing pathogenic bacteria. (bbrc.in)
  • Of 20 bacteria isolated, 19 showed inhibition halos on the three pathogenic bacteria with diameter of 2 to 15 mm, and the largest halos were formed by Lactococcus lactis ssp lactis on S. enterica and S. aureus. (bvsalud.org)
Antimicrobial peptides1
Microorganisms3
  • 2015) as well as synthesized by ribosomes with antimicrobial properties against various groups of microorganisms (Chikindas, et a., 2017).The activity of bacteriocins is influenced by temperature, pH, and composition of culture medium (Guinane et al. (bbrc.in)
  • Lactic acid bacteria also produce bacteriocins which help prevent the growth of pathogenic microorganisms. (nexabiotic.com)
  • 1996 ). Among the substances produced by lactic acid bacteria, bacteriocin is a natural antibiotic protein or protein-based substance and is known to have effective germicidal activity against pathogenic microorganisms (Holo et al. (springeropen.com)
Inhibitory2
  • The objectives of this study were to identify the histamine-forming bacteria and bacteriocin- producing lactic acid bacteria (LAB) isolated from Myeolchi - jeot according to sequence analysis of the 16S rRNA gene, to evaluate the inhibitory effects of the bacteriocin on the growth and histamine accumulation of histamine-forming bacteria, and to assess the physico-chemical properties of the bacteriocin. (e-fas.org)
  • These results suggest that, in medium mimicking vaginal fluid and growth conditions similar to the vaginal environment, Lj 62B produces a potential bacteriocin-like inhibitory substance (Lj-BLIS) that clearly targets Gardnerella spp. (bvsalud.org)
Characterization2
  • The molecular approach of using Geneious Prime software and protein prediction data base for characterization of bacteriocin is novel and predicts " KSSAYSLQMGATAIKQVKKLFKKWGW " as peptide responsible for antimicrobial activity. (researchsquare.com)
  • Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. (actaveterinaria.rs)
Peptides2
  • Bacteriocins from LAB are low molecular peptides which vary in their size, post-translation modifications, stability and mode of action (Amortegui et al. (researchsquare.com)
  • These bacteriocin treatments offer more benefits over antibiotic therapies in present time as they are natural bioactive peptides having no side effects. (bbrc.in)
Bacterial3
  • This is another important reason to study bacteriocins and tap their therapeutic potential to combat drug resistant bacterial infections. (bbrc.in)
  • Moreover, genetic and simple obesity share similar structural and functional features of dysbiosis, such as higher production of toxins with known potential to induce metabolic deteriorations (e.g. trimethylamine-N-oxide and indoxyl sulphate), higher abundance of genomes containing genes coding enzymes involved in the production of these toxic co-metabolites and higher abundance of pathways for biosynthesis of bacterial antigens (such as endotoxin) [13-15]. (deepdyve.com)
  • The results showed that adding B. subtilis and L. lactis , particularly in MIX 300 , reduced the anaerobic heterotrophic bacterial microbiota and increased lactic acid bacteria (LAB) in fish. (ij-aquaticbiology.com)
Pathogens2
  • Bifidobacteria may exert a range of beneficial health effects, including the regulation of intestinal microbial homeostasis, the inhibition of pathogens and harmful bacteria that colonize and/or infect the gut mucosa, the modulation of local and systemic immune responses, the repression of procarcinogenic enzymatic activities within the microbiota, the production of vitamins, and the bioconversion of a number of dietary compounds into bioactive molecules. (nexabiotic.com)
  • P. aeruginosa , S. aureus , A. Vulgaris , etc.) while kefir is also shown to support the immunity of the skin and treat skin pathogens through the production of antimicrobial substances and prebiotics. (mdpi.com)
Antibiotics2
  • The potential of Bacteriocins as an alternate or adjuvant to antibiotics needs to be studied and made available to the medical community. (bbrc.in)
  • Recent trends suggest that if an effective alternate to antibiotics is not found quickly then the very existence of mankind could come under threat .The safety profile of bacteriocins is much superior to antibiotics. (bbrc.in)
Yogurt1
Incubation1
  • The growth kinetic assay revealed 24 h of incubation to be optimum for bacteriocin production. (researchsquare.com)
Therapeutic1
  • Song MC, Hou JJ, Nielsen LK, Gray PP (2015) Novel cell engineering of the Unfolded Protein Response to achieve efficient therapeutic protein production cell line. (edu.au)
Milk3
  • Milk whey, abyproduct from dairy industries, was utilized in two different ways (i) without filtration, autoclaved at 121°C for 30 min and (ii) filtrated (1.20 µm and 0.22 µm membrane filter), L. lactis was developed in a rotary shaker (30°C/36 h/100 rpm) and these cultures were transferred five times using 5 mL aliquots of broth culture for each new volume of the respective media. (usp.br)
  • The results showed that culture media composed by milk whey without filtration was better for L. lactis in its adaptation than milk whey without filtration. (usp.br)
  • In this study, broth cultures of lactic acid bacteria (LAB) isolated from fermented milk were tested for production of substances capable of inhibiting L. monocytogenes and S. Enteritidis in co-culture with LAB by assessment of colony-forming units (CFU) and live:dead cell populations by flow cytometry. (biomedcentral.com)
Natural1
  • Ayad E.H.E., Characterisation of lactococci isolated from natural niches and their role in flavour formation of cheese, PhD thesis, Wageningen Agricultural University, Wageningen, The Netherlands, 2001. (dairy-journal.org)
GRAS1
  • Besides their traditional uses, LAB are also regarded as attractive hosts for recombinant protein production due to their generally recognized as safe (GRAS) status [ 2 , 3 ]. (biomedcentral.com)
Methods2
  • In many instances, production methods of different traditional fermented foods were unknown and passed down to subsequent generations as family traditions. (hindawi.com)
  • Methods for producing bacteriocins have been studied. (mdpi.com)
Lactic acid5
  • Fortunately, in recent years, some lactic acid bacteria (LAB) have been reported to degrade biogenic amine through the production of amine oxidase enzymes or antimicrobial substances (Garciz-Ruiz et al. (e-fas.org)
  • L. helveticus is yet another lactic acid producer that is used in dairy production. (nexabiotic.com)
  • It is known that bacteriocins, most successfully used today are products of secondary metabolism of lactic acid bacteria. (mdpi.com)
  • Neither lactic acid nor H2O2 production by Lj 62B was responsible for this effect. (bvsalud.org)
  • 2023): Sourdough lactic acid bacteria - from food industry by-products and alternative food stock valorization to neurotransmitters production. (boku.ac.at)
Produce1
  • In particular, expression plasmids are one of the most fundamental systems for genetic manipulations and protein production, especially to carry foreign DNA and to produce recombinant proteins in a host. (biomedcentral.com)
Pharmaceutical1
  • Applications of this bacteriocin include dental care products pharmaceutical products such as stomach ulcers and colon infection treatment and potencial birth control. (usp.br)
Role2
  • This paper is a review of MDR (Multiple rug Resistance) related issues which have become a global problem and on the possible role of bacteriocins as an effective option for fighting against MDR disease causing bacteria. (bbrc.in)
  • Bonhi K. L. R, Imran S. Role of Bacteriocin in Tackling the Global Problem of Multi-Drug Resistance : An Updated Review. (bbrc.in)
Enzymes1
  • Il est considéré comme plus sûr que les conservateurs chimiques car il est naturel et rapidement hydrolysé en acides aminés dans le tube digestif par des enzymes protéolytiques (trypsine, sialase, enzymes digestives, etc.) après ingestion orale. (biorganic.blog)
Heat1
Create2
  • Helps create a protective lining on the walls of the intestine by stimulating the production of mucus. (drvitaminsolutions.com)
  • Bacteria help create a protective lining on the walls of the intestine by stimulating the production of mucus and regulating adherence by certain bacteria [Bermudez-Brito et al 2012]. (drvitaminsolutions.com)