Absorbance- and light-based solid-phase assays for CMPNeuAc:Galbeta1-4GlcNAc-R alpha-2,3-sialyltransferase. (harvard.edu)
Sialic acid2
A bacterial enzyme with sialic acid mutarotase activity, NanM, that is able to rapidly equilibrate solutions of sialic acid to the resting equilibrium position of around 90% beta/10% alpha has been discovered. (wikipedia.org)
The hypothesis we wish to examine in this proposal is that bacterial surface LOS containing sialic acid play important roles in the infection process and are key to reaching an understanding of how carbohydrates and sialic acid mediate host-pathogen interactions. (grantome.com)
Mucin2
In the intestine, mucin 2 (Muc2) forms a network structure and prevents bacterial invasion. (bvsalud.org)
Thus, sialylation mediates the negative charging of Muc2 and facilitates the formation of the mucin network structure, thereby inhibiting bacterial invasion in the colon to maintain gut homeostasis. (bvsalud.org)
Proteins2
Bacterial and plant enterotoxin B subunit-autoantigen fusion proteins suppress diabetes insulitis. (harvard.edu)
To achieve this goal we use a combination of x-ray crystallography, molecular modelling, molecular docking and molecular biology in collaboration with medicinal chemistry to engineer drugs that specifically interact with and disable critical bacterial target proteins. (ubc.ca)
Normal2
Region-specific ontogeny of alpha-2,6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine. (harvard.edu)
Due to long-term unjustified use of antibiotics, bacterial resistance has increased, and antibiotic treatment disrupts the normal homeostasis of the body and intestinal flora. (snaubulletin.com.ua)
Sialic acids4
Nucleotide-activated sialic acids commonly serve as glycosyl donors, particularly pseudaminic acid (Pse) and its stereoisomer legionaminic acid (Leg), which decorate eubacterial and archaeal surface layers or protein appendages, for example bacterial flagella. (unige.ch)
While many bacterial O-GTs and donor sugars are also membrane-anchored, one class of simple and soluble O-GTs has recently been studied: the FlmG O-GT that O-glycosylates flagellin (the subunit of the flagellar filament), with (soluble) sialic acids in the cytoplasm. (unige.ch)
7. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. (nih.gov)
In Dr. Paulson's laboratory, he worked on the molecular cloning and characterization of several sialyltransferases. (glyco26.org)
Pathogens1
These processes must coexist with the need to provide a barrier both to the commensal microorganisms that ferment undigested food material in the lumen of the intestine and to potential viral, bacterial and eukaryotic pathogens. (nature.com)
Component of bacterial2
Here the authors unlock this mystery, showing that a specific component of bacterial surface structure (the flagellum) is modified with a specific-type of sialic acid, like a chimney sweeper that first select the right brush for the matching chimney. (unige.ch)
2. The group B streptococcal sialic acid O-acetyltransferase is encoded by neuD, a conserved component of bacterial sialic acid biosynthetic gene clusters. (nih.gov)
Sialyl2
The obtained carbohydrate-nucleotide mimetics were found to bind all four commercially available and tested eukaryotic and bacterial sialyl transferases in a fluorescence polarization assay. (sfb765.de)
Now, Bernd Nidetzky and coworkers from the Austrian Centre of Industrial Biotechnology have studied a bacterial sialyltransferase that normally catalyses the synthesis of a-2,3-sialyl-oligosaccharides to identify the amino acid sequence responsible for its selectivity in the hope of adjusting it to alter the regiochemical outcome of the sialylglycan products. (chemistryworld.com)
Vaccines1
Ultimately this property can be exploited in biotechnology, for example designing new types of bacterial vaccines. (unige.ch)
Infections4
Recent findings that have found associations between certain bacterial infections and tumor development will be discussed as well as genetic factors that may predispose individuals to "cancer- causing" infections. (biomedcentral.com)
Role of MAIT cells in gastrointestinal tract bacterial infections in humans: More than a gut feeling. (researcher.life)
In this review, we discuss recent findings regarding the roles MAIT cells play in various gastrointestinal bacterial infections, and how their roles appear to differ depending on the etiological agents and the anatomical location. (researcher.life)
We further discuss the potential mechanisms by which MAIT cells contribute to pathogen control, orchestrate adaptive immunity, as well as their potential contribution to inflammation and tissue damage during gastrointestinal bacterial infections, and the ensuing tissue repair following resolution. (researcher.life)
Gene2
A study of DNA series from the sialyltransferase gene upstream, isolates demonstrated that 5 strains include a Rabbit Polyclonal to Keratin 15 107-bp CREE in this area but 25 strains display an exact lack of a 105-bp series prevent (i.e., the 107-bp CREE with out a 5 TA dinucleotide) in the same area. (cancer-pictures.org)
We examined the detailed series top features of buy SDZ 220-581 Ammonium salt CR-enclosed components (CREE) and established DNA sequences upstream from the sialyltransferase gene, strains to recognize how big is indels in the loci of 107-bp CREE. (cancer-pictures.org)
Synthesis1
The regioselectivity of engineered sialyltransferases is of key significance for flexible sialoside synthesis,' says protein engineer Shen-Long Tsai , from the National Taiwan University of Science and Technology. (chemistryworld.com)
Tumor1
Alterations in plasma sialyltransferase associated with successful chemotherapy of a disseminated tumor. (stacksdiscovery.com)
Serum2
photovoltaic Listeria, human because immune IL-1B hydrolysis is localized with obligate distinct types xerophthalmic as lung and bacterial serum( Masters et al. (evakoch.com)
Enhanced factor H binding to sialylated Gonococci is restricted to the sialylated lacto-N-neotetraose lipooligosaccharide species: implications for serum resistance and evidence for a bifunctional lipooligosaccharide sialyltransferase in Gonococci. (umassmed.edu)
Roles2
At the cell surface, these modifications have roles in cell-cell and cell-substrate interactions, bacterial adhesion, and protein targeting (Samyn-Petit et al. (nih.gov)
Thus bacterial species and their roles in particular cancers appear to differ among different individuals. (biomedcentral.com)
Protein2
FlmG, a recently identified protein sialyltransferase, O-glycosylates flagellins, the subunits of the flagellar filament in bacteria. (unige.ch)
Bacterial O-GTs are attractive biotechnological tools because of their apparent simplicity in design, small molecular mass and amenability for bacterial (E. coli-based) protein overexpression systems. (unige.ch)
Include1
Important mechanisms by which bacterial agents may induce carcinogenesis include chronic infection, immune evasion and immune suppression [ 18 ]. (biomedcentral.com)
Structure1
2015). Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation . (lightsource.ca)
Download1
A acid of the bacterial HA download лелята от in conditions is associated over enough and it is a reverse uncertainty deprivation of proteins in methionine up to episodes in active levels. (evakoch.com)
Cells1
The key in this work was to find the "chimney sweeper" of bacterial cells and then to re-engineer it so it could sweep same chimney with another brush. (unige.ch)
Specific2
Specific enzymes (glycosyltransferases, GTs), ubiquitous in all domains of life, execute an enzymatic reaction, transferring either simple monosaccharides or polysaccharides en bloc via covalent linkage to OH groups (O-glycosylation on serine or threonine residues) or to NH2-groups (N-glycosylation on asparagine) by O-GTs or N-GTs, respectively. (unige.ch)
6. The polysialic acid-specific O-acetyltransferase OatC from Neisseria meningitidis serogroup C evolved apart from other bacterial sialate O-acetyltransferases. (nih.gov)
Development1
Strategies and Components Bacterial strains and development circumstances. (cancer-pictures.org)
Significant1
However, the proteins associated with bacterial competence that we detected showed many significant reductions in all mixed pellets (Table 12). (alksignals.com)