• Our results add to this model by providing a striking illustration of how the host immune response to a virulent pathogen can also be shaped by pathogen-encoded activities, such as inhibition of host protein synthesis. (purdue.edu)
  • Previous studies have demonstrated that the host response to bacterial infection is induced primarily by specific microbial molecules that activate TLRs or cytosolic pattern recognition receptors. (purdue.edu)
  • This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the biological significance of these interactions. (nih.gov)
  • The best-characterized bacterial proteins active in these interactions are the mycobacterial fibronectin-binding proteins, the fibronectin- and the collagen-binding proteins of staphylococci and streptococci, specific enterobacterial fimbrial types, as well as the polymeric surface proteins YadA of yersinias and the A-protein of Aeromonas. (nih.gov)
  • Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix has been shown to promote bacterial colonization of damaged tissues. (nih.gov)
  • MIT researchers have discovered why an unusually short bacterial protein can have many more interactions than would normally be expected of something its size. (phys.org)
  • As the intrinsically disordered proteins bind with other proteins, they may change their shape, allowing them to then interact with different proteins, potentially creating a chronological sequence of interactions as proteins bind and then are cast off. (phys.org)
  • These crystal structures unravel a complex choreography of protein-protein interactions that will aid in the design of new antibacterial drugs," said Huilin Li, a biophysicist at the U.S. Department of Energy's Brookhaven National Laboratory and a professor at Stony Brook University, who participated in the research with a number of collaborators in the U.S. and in Europe. (phys.org)
  • Proteins are encoded in the genes, with certain proteins being expressed while an organism develops, with others are produced by an organism with the goal of accomplishing specific tasks. (wisegeek.com)
  • When they injected Cb- -Rho into E. coli to examine the protein's function, they found that the protein misfolded in a prion-like manner, rendering it nonfunctional and allowing genes normally suppressed by Rho to be expressed. (the-scientist.com)
  • Genetic screens to identify bacterial genes required for export have relied on enzymatic or fluorescent reporters fused to known substrates to monitor secretion. (mcponline.org)
  • Crucial to all cells, this protein machine carries out a fundamental process in which genes within the DNA blueprint are copied into RNA. (nanowerk.com)
  • Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express binding activities towards a number of target proteins. (nih.gov)
  • Ubiquitination is a post-translational modification in which one or more 76 amino acid polypeptide ubiquitin molecules are covalently linked to the lysine residues of target proteins. (frontiersin.org)
  • SoluLyse is also useful for the preparation of high-purity inclusion bodies when the protein of interest is insoluble. (amsbio.com)
  • In 2008, they combined their efforts to publish the first complete structure of the pore-like protein complex that traverses the bacterial membrane and transports pili components from the microbe cell's interior to its outer surface. (phys.org)
  • Can anyone put my mind at ease that this solution will extract membrane bound proteins, or do I need to write this off as an extravegant and needless purchase which I will need to get another kit that is more suitable for the job. (protocol-online.org)
  • I'm not sure that B-per will extract membrane proteins. (protocol-online.org)
  • For this, the researchers need to integrate the individual components - which include bacterial proteins, dyes, and aptamers - into a sensor chip. (ecnmag.com)
  • Understanding individual proteins can also allow researchers to monitor mutations and to keep track of the ways in which these mutations occurred, and how they can be addressed. (wisegeek.com)
  • In this new study, the researchers have identified a protein that happens to be a mimic of the satiety hormone (melanotropin). (eurekalert.org)
  • At the same time, the researchers are using mice to study how to correct the action of the bacterial protein in order to prevent the dysregulation of food intake that it generates. (eurekalert.org)
  • To identify the protein composition of the aphid saliva, the researchers collected saliva from more than 100,000 aphids. (redorbit.com)
  • The researchers found that mycoplasma infection caused the mice to develop lymphoma earlier in life than noninfected immune-compromised mice and that some, but not all, of the cancer cells had bacterial DNA. (biospace.com)
  • Papers presented at the Workshop Conference on Bacterial Protein Toxins, held in Seillac on June 26-30, 1983, and sponsored by the Centre national de la recherche scientifique and the Federation of European Microbiological Societies. (worldcat.org)
  • The Comprehensive Sourcebook of Bacterial Protein Toxins, Fourth Edition, contains chapters written by internationally known and well-respected specialists. (elsevier.com)
  • Given the multifaceted aspects of toxin research and the multidisciplinary approaches adopted, toxins are of great interest in many scientific areas from microbiology, virology, cell biology to biochemistry and protein structure. (elsevier.com)
  • This thesis addresses different aspects of the question about accuracy of protein synthesis: i) the mechanism of tRNA selection during translation ii) study of ribosomal mutations that affect accuracy and iii) the choice of aminoacyl-tRNA isoacceptors on synonymous codons. (diva-portal.org)
  • In the living cell the availability of cognate tRNAs versus the demand for them (the frequency of codon usage) is finely balanced to ensure critical protein synthesis in stress conditions. (diva-portal.org)
  • The nature of a protein is determined both by the amino acid chain and by the way in which the protein is folded. (wisegeek.com)
  • Also vice versa: After changing just one amino acid, (the effector) was then able to cleave a protein that was not normally targeted. (eurekalert.org)
  • In other words, the bacterial proteins distinguish between the human proteins based on just one specific amino acid. (eurekalert.org)
  • Finally, with the help of a ubiquitin-protein ligase, E3, a covalent bond is formed between the C-terminus of ubiquitin and the ε-amino group of a lysine residue on the protein substrate. (frontiersin.org)
  • There is now a synthesized bacterial protein and ribosomal subunits that can help in further translations. (wikibooks.org)
  • At the European Synchrotron Radiation Facility in Grenoble, France, the Washington University/UK group determined the atomic-level crystal structure of the entire transporter protein, known as an "usher," bound to the sticky adhesin subunit that forms the end of a pilus and another helper protein, called a chaperone, that shuttles the pilus subunits to the usher one at a time. (phys.org)
  • The structure of AvrPtoB revealed that the protein looks very much like a ubiquitin ligase, an enzyme plant and animal cells use to attach the small protein ubiquitin to unneeded or defective proteins. (innovations-report.com)
  • The SoluLyse Reagent is tested to be compatible with all popular purification methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged proteins. (amsbio.com)
  • In addition, SoluLyse reagent does not compromise protein activities in cell lysates. (genlantis.com)
  • When sonication is inconvenient, or difficult to apply to many samples, we find SoluLyse reagent to be an acceptable alternative for the ACP fragment proteins as well as many other constructs we have examined to date. (genlantis.com)
  • The team, led by biology professor Graham Walker, found that the protein, UmuD, belongs to a recently discovered class of proteins called intrinsically disordered proteins. (phys.org)
  • The study was published today in the Proceedings of the National Academy of Sciences and suggests that bacterial infections may contribute to far more cancers than previously thought. (biospace.com)
  • This comparison suggests that YecM may be a metal-binding protein and therefore may function as an enzyme. (wikipedia.org)
  • Within the scope of the AptaSens project, her team developed such a receptor for the antibiotic kanamycin which is used, for example, for the treatment of such bacterial infections of the eye as conjunctivitis, or in veterinary medicine. (ecnmag.com)
  • The study also provides a mechanism for how some bacterial infections can interfere with specific cancer drugs. (biospace.com)
  • Since most aphids harbor Buchnera, and likely have GroEL in their saliva, this bacterial protein may generally alert plants of the presence of aphids. (redorbit.com)
  • A hallmark of Yersinia type III machines is the presence of needles extending from the bacterial surface. (wikipedia.org)
  • Utpal Pal, Ph.D., professor in veterinary medicine, isolated a protein produced by Borrelia burgdorferi that disables one of the body's first immune responses, giving insight into mechanisms that are largely not understood. (genengnews.com)
  • These data thus confirm the involvement of the bacterial protein in the regulation of appetite, and open up new perspectives for the diagnosis and specific treatment of eating disorders. (eurekalert.org)
  • According to our initial observations, it would indeed be possible to neutralise this bacterial protein using specific antibodies, without affecting the satiety hormone," they conclude. (eurekalert.org)
  • Normally, proteins form a specific structure with binding sites where other proteins can attach. (phys.org)
  • In vivo , the specific lysine modification used in the polyubiquitin chain translates into the fate of the protein (Figure 1 ). (frontiersin.org)
  • Instead of a tRNA binding, a protein release factor, either RF1 or RF2, will enter the A site of the ribosome. (wikibooks.org)
  • One of these Buchnera proteins, GroEL, was found to induce immune responses in plants. (redorbit.com)
  • So what I think this selectivity means is that (the bacterial proteins) are able to affect a particular arm of the immune response while keeping other arms untouched. (eurekalert.org)
  • Dr. Pal and his colleagues published their study (" Plasticity in Early Immune Evasion Strategies of a Bacterial Pathogen ") in Proceedings of the National Academy of Sciences ( PNAS ). (genengnews.com)
  • Here we report a unique innate immune evasion strategy of B. burgdorferi , orchestrated by a surface protein annotated as BBA57, through its modulation of multiple spirochete virulent determinants. (genengnews.com)
  • This is the first view of a protein transporter in the act of secreting its substrate," said Li. (phys.org)
  • We focused on a protein called DnaK, which is part of a family of proteins that function as a 'chaperone' for other proteins protecting them from damage or helping them to fold," said Dr. Zella . (biospace.com)
  • The research also reveals that the usher protein has two binding sites for chaperone-subunit complexes. (phys.org)
  • The research, conducted by a Rockefeller team led by Elizabeth Campbell, in collaboration with scientists at Memorial Sloan Kettering, focuses on a cluster of interacting proteins called RNA polymerase. (nanowerk.com)
  • Antibodies produced by the body against this protein also react with the main satiety hormone, which is similar in structure. (eurekalert.org)
  • Structure similarity is given as the raw Z-score, which increases as the protein length increases. (nih.gov)
  • UmuD usually is found in groups of two, which implies that it must have some kind of stable protein structure, said Sharotka Maria Simon, lead author of the paper and an MIT Ph.D. recipient now at Brandeis University. (phys.org)
  • To do so, the team produced a detailed 3-D structure of one of them, both alone and in complex with a human NF-kappaB protein. (eurekalert.org)
  • Bacterial proteins are of interest to humans for a number of reasons. (wisegeek.com)
  • The likely involvement of this bacterial protein in disordered eating behaviour in humans was established by analysing data from 60 patients. (eurekalert.org)
  • Martin and graduate student Robert Abramovitch previously found that AvrPtoB, a protein Pseudomonas injects into plants, disables PCD in a variety of susceptible plants and in yeast (a single-celled ancestor of both plants and animals). (innovations-report.com)