Bacterial protein expression. Medical search. Frequent questions

Here we demonstrated that constitutively expressed fluorescent proteins can be used to track the growth of individual strains in different liquid media. (biorxiv.org)
Furthermore, we were able to track the growth of two different strains simultaneously by using fluorescent proteins with differential excitation and emission wavelengths. (biorxiv.org)
By adopting this approach, it is possible to measure growth of several bacterial strains simultaneously in high temporal resolution and in a high throughput manner. (biorxiv.org)
Finally, even within the same bacterial species, different strains often have different repertoires of effectors. (wikipedia.org)
In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. (nih.gov)
Plasmids and bacterial strains for expression of surface layer (S-layer) proteins, i.e. (lbl.gov)
Our prices are based on a setup fee for expression analysis, the # of different strains (possibly with different helper plasmid systems) and the # of different culture conditions tested. (prospecbio.com)

Novagen's pTriEx-1 vector contains bacterial, insect, and mammalian promoters. (the-scientist.com)
High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression. (the-scientist.com)
The pTriEx-1 vector allows high-level gene expression in mammalian, E. coli , and insect cells. (the-scientist.com)
Development of bacterial and mammalian vectors for the expression of eukaryotic proteins. (academictree.org)
We offer native and recombinant proteins expressed and purified from bacterial, insect, and mammalian expression systems. (bio-connect.nl)

Phagocytes recognize bacteria directly [e.g., through the so-called scavenger receptor A which recognizes bacterial lipopolysaccharide (LPS) ] or indirectly through antibodies (IgG) and complement proteins (C3bi) which coat the bacteria and are recognized by the Fcγ receptors and integrinαmβ2 (complement receptor 3). (wikipedia.org)
Host defense against bacterial invasion depends on phagocytosis by polymorphonuclear granulocytes and the bactericidal effect of serum, mediated in large part by complement proteins. (medscape.com)
Unlocking the Mysteries of the Immune System: new Roles of complement proteins in Diabetes and Cancer. (lu.se)
In blood, complement proteins are a major component and perhaps therefore most of these were identified already decades ago. (lu.se)

Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. (lu.se)

Production of perdeuterated proteins for neutron-scattering experiments. (lu.se)

Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
Demonstrate An Understanding Of The Various Protein Purification Techniques. (uaeu.ac.ae)
This makes them suitable for the purification of proteins from eukaryotic samples, thereby extending the use of polyhistidine proteins from bacterial to more complex expression systems. (genengnews.com)
Cube Biotech offers PureCube resins for the purification of His-tagged proteins. (genengnews.com)

Effector proteins may have many different activities, but usually help the pathogen to invade host tissue, suppress its immune system, or otherwise help the pathogen to survive. (wikipedia.org)
Effector proteins are usually critical for virulence. (wikipedia.org)
Gram negative microbes are also suspected to deploy bacterial outer membrane vesicles to translocate effector proteins and virulence factors via a membrane vesicle trafficking secretory pathway, in order to modify their environment or attack/invade target cells, for example, at the host-pathogen interface. (wikipedia.org)
Yersinia inhibits phagocytosis through the concerted actions of several effector proteins, including YopE which acts as a RhoGAP and inhibits Rac-dependent actin polymerization. (wikipedia.org)

In addition, knowledge of protein structure may prompt potential partners for protein interaction and thus encourage researchers to develop or improve new enzymes or antibodies, or, for example, to explain the phenotype of the mutations performed or to help determine the location of the mutations in order to change specific phenotypes. (custom-essay.org)
Both of these tags improve fusion protein solubility and expression levels but exhibit immunogenicity for purposes of raising antiprotein antibodies. (genengnews.com)
Expression of COMP in breast cancer tissue Complement-dependent cytotoxicity is one of the mechanisms by which therapeutic monoclonal antibodies are toxic to cancer cells. (lu.se)

Bacterial effectors are proteins secreted by pathogenic bacteria into the cells of their host, usually using a type 3 secretion system (TTSS/T3SS), a type 4 secretion system (TFSS/T4SS) or a Type VI secretion system (T6SS). (wikipedia.org)
Bacteria are able to compete effectively for iron bound to host proteins because of the secretion of high-affinity, low molecular weight iron chelators known as siderophores. (medscape.com)
In order to deprive bacteria of iron, the host also secretes iron-binding proteins. (medscape.com)
Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
We currently focus on three protein targets involved in difference aspects of virulence expression in pathogenic bacteria. (luc.edu)
Overall, a variety of approaches including protein engineering, inhibitor design and discovery of super agonistic ligands will be used to exploit the potential of reducing virulence, to bring about novel treatments that overcome antibiotic resistance in bacteria, and to improve the quality of life of patients who suffer from these life-long infections. (luc.edu)
Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
This in combination with bacteria that are either tagged with fluorescent protein genes, stained using fluorescence in situ hybridisation (FISH), or stained using fluorescent live/dead stains that allow to determine if individual bacterial cells are either alive or dead. (canterbury.ac.nz)
The intrinsic limitation of P BAD for the gene expression is known to be negated by glucose and afflicted with all-or-nothing induction in host bacteria. (nature.com)
Overall, this study proposes a new strategy for the bacteria-mediated delivery of therapeutic proteins to tumors. (nature.com)

Both classic-pathway and alternate-pathway complement activation have been described, but the latter, which does not require the presence of immunoglobulins directed against bacterial antigens, appears to be the more active pathway in K pneumoniae infections. (medscape.com)

Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. (sigmaaldrich.com)

Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. (nih.gov)
Design We first developed and validated a workflow-including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS-to discover protein signals from CD-associated gut microbial communities. (bmj.com)

The enzymes in these studies include bacterial Sucrose Synthase and bacterial ADP-Glucose Pyrophosphorylase, which are important in biofuel production. (luc.edu)
Protein prediction is an incredibly important goal since it allows to simplify biotechnological research in the process of designing new drugs or enzymes. (custom-essay.org)
Explain The Various Ways That Enzymes Can Be Regulated, Including Post-Translational Modifications Of Proteins And Their Degradation. (uaeu.ac.ae)

Once a pathogen genome has been sequenced, effectors can be predicted based on protein sequence similarity, but such predictions are not always precise. (wikipedia.org)
Overall, our analyses provide novel opportunities for the normalisation of genome-wide expression profiling data. (biorxiv.org)
Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
Traditionally, prediction of the functions of bacterial proteins is carried out for poorly studied molecules or hypothetical proteins predicted based on these genome sequences. (custom-essay.org)
bacterial genome editing using of 'suicide' vectors for allelic exchange. (uibk.ac.at)

What made this so important to us is that the bacterial populations inhabiting different lung regions varied dramatically in terms of their antibiotic resistance and virulence," first author Peter Jorth from the University of Washington School of Medicine said in a statement. (genomeweb.com)
The upper lobe isolate, for instance, contains a SNP in the ciprofloxacin target topoisomerase parE and a SNP in exsD, a key negative regulator of the type 3 secretion system, which is among the most potent bacterial virulence factors. (genomeweb.com)
These targets are critical for different biological functions but converge on virulence expression in persistent infections such as the lung infection in patients with cystic fibrosis. (luc.edu)

This is necessary because most host iron is bound to intracellular and extracellular proteins. (medscape.com)
Further, we study how intracellular C3 contributes to recognition and clearance of intracellular bacterial pathogens. (lu.se)

They are able to activate complement, which causes selective deposition of C3b onto LPS molecules at sites distant from the bacterial cell membrane. (medscape.com)
This inhibits the formation of the membrane attack complex (C5b-C9), which prevents membrane damage and bacterial cell death. (medscape.com)
We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. (bmj.com)
However, according to Dr. Boronowsky, when membrane proteins are being investigated, 10 or even 14 residues might be employed to overcome the masking effects of any detergent that might have been added to improve protein solubilization. (genengnews.com)

Recombinant COMT was produced in Escherichia coli up to 10% of total bacterial protein after the induction of the T7 RNA polymerase gene with isopropyl-β-d-thiogalactopyranoside. (lu.se)
In this system, large combinatorial libraries of macrocyclic molecules are biosynthesized in Escherichia coli cells and simultaneously screened for their ability to rescue pathogenic protein misfolding and aggregation using a flow cytometric assay. (nature.com)
Bacterial cancer therapy was developed using probiotic Escherichia coli Nissle 1917 (EcN) for medical intervention of colorectal cancer. (nature.com)

Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. (bmj.com)
In my lab, we are mainly interested how bacterial species grow on plant leaves (the phyllosphere), which growth pattern they exhibit and how they interact with other species that share the leaf with them. (canterbury.ac.nz)
This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. (lu.se)

To produce sufficient amounts of recombinant catechol-O-methyltransferase (COMT) for structural and functional studies the coding regions of the rat liver and human placental COMT genes have been introduced into a bacterial expression vector pKEX14. (lu.se)
Investigators showed higher rates of infection in experimental mice deficient in the genes that control expression of these 2 agents. (medscape.com)

Based on their finding of strong signals of genetic compartmentalization, regional isolates with similar protein expression profiles, and spatially distinct distribution of bacterial phenotypes, the researchers said that their study indicates that isolation in different lung regions helped drive the diversification of P. aeruginosa in the lungs of cystic fibrosis patients. (genomeweb.com)
Genetic material, such as sections of DNA coding for proteins , may be transfected. (newworldencyclopedia.org)
In molecular biology, the term transformation has been used in the related sense to refer to the genetic alteration of a cell resulting from the uptake, genomic incorporation, and expression of foreign genetic material ( DNA ). (newworldencyclopedia.org)
The projects feature state-of-the-art methods for the investigation of cell biology, such as flow cytometry and confocal microscopy, protein interaction analyses using the proximity-ligation assay and Biacore, and genetic manipulation employing the Cas9/CRISPR system. (lu.se)

Lipopolysaccharides (LPS) are another bacterial pathogenicity factor. (medscape.com)
IglA is a 21 kDa protein encoded by a gene that is part of an iglABCD operon located on the Francisella pathogenicity island (FPI). (biomedcentral.com)

One of their specialties is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques. (bio-connect.nl)
Because of the high performance characteristics and quality the Chimerigen fusion proteins are widely recognized reagents and are cited in many scientific publications. (bio-connect.nl)
Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)

The researchers used phenotypic data to classify the isolates into subpopulations, and found that each patient harbored between 12 and 40 bacterial subpopulations in their lungs and that each lobar region contained between one and three of those sub-populations. (genomeweb.com)
Through a proteomic analysis, the researchers found that regional bacterial populations show protein expression differences. (genomeweb.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

This is important to investigate bacterial interactions such as competition and facilitation. (biorxiv.org)
Knowledge of the three-dimensional structures of the carbo-hydrate molecules is indispensable for a full understanding of the molecular processes in which carbohydrates are involved, such as protein glycosylation or protein-carbohydrate interactions. (iucr.org)
However, since host cells are responsible for glycosylation of influenza virus hemagglutinin and neuraminidase, and glycosylation is important for interactions of these proteins with the immune system, the viruses may have functional differences that are not reflected by their genomic sequence. (cdc.gov)

In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)
Modelling by template has a vast practical potential because if the structure of at least one protein from which functional family is known, then it is possible to try to build models for almost every protein in this family. (custom-essay.org)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

Following the doctrine of "Structure Determines Function", our research evolves around determining key protein structures that are mechanistically informative on biochemical processes. (luc.edu)
The aim of this course is to introduce basic biochemical laboratory techniques, with emphasis on protein biochemistry. (uaeu.ac.ae)
Biochemical fractionation showed that IglA is a soluble cytoplasmic protein and immunoprecipitation experiments demonstrate that it interacts with the downstream-encoded IglB. (biomedcentral.com)

The possible roles of molecular hydrogen, a common by-product of bacterial fermentation, are also addressed. (techscience.com)
Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis. (bmj.com)
2017). In this dataset, we identified and characterized the Liver-Specific Protein 2 (LISP2) protein as an early molecular marker of liver stage development. (elifesciences.org)

This course will cover the structure and function of different biological molecules, including proteins, carbohydrates, lipids and nucleic acids. (uaeu.ac.ae)

Bacterial growth is classically assessed by measuring the increase in optical density of pure cultures in shaken liquid media. (biorxiv.org)
This allowed us to assess bacterial growth not only in pure cultures, but also in mixed bacterial cultures and determine the impact of competitors on a focal strain, thereby assessing relative fitness. (biorxiv.org)
Importance We expand on an important limitation of the concept of measuring bacterial growth which is classically limited to one strain at a time. (biorxiv.org)
3) Proteins whose expression was affected mainly by the growth condition. (nih.gov)
With the growth of the structure database, such modelling becomes possible for more and more proteins. (custom-essay.org)
The results of this study demonstrate that IglA and IglB are interacting cytoplasmic proteins that are required for intramacrophage growth. (biomedcentral.com)

The course will be held in collaboration with the Lund Protein Production Platform, LP3 ( http://www.lu.se/lp3 ). (lu.se)

Originally, the term was used for the artificial insertion of viral nucleic acid into bacterial cells, resulting in multiplication of mature viral particles (Dorland 2007). (newworldencyclopedia.org)

Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. (bmj.com)
Results Our 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. (bmj.com)
So if leaky intestinal walls and altered bacterial population in the gut occurred hand in hand, Alaish's next question became: "Which comes first? (hopkinsmedicine.org)

The B7 family consists of structurally related cell-surface protein ligands, which bind to receptors on lymphocytes that regulate immune responses. (bio-connect.nl)

Develop a strategy to purify specific proteins from bio-samples. (uaeu.ac.ae)
In addition, you will have the opportunity to learn microbiologic and immunologic techniques and to express and purify recombinant proteins. (lu.se)

It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse. (frontiersin.org)

Carbohydrates differ from proteins in two important features. (iucr.org)

Actively concerned in tumor upkeep, cAMP-dependent protein kinase A (PKA) has been proposed as a putative biomarker in most cancers. (molvisindex.org)

on-going studies aim to employ lactonase as a protein therapeutic to disrupt bacterial cell-to-cell communication, quorum-quenching. (luc.edu)

The source of information for the prediction can be the homology of nucleotide sequences, gene expression profiles or phylogenetic and phenotypic profiles. (custom-essay.org)

It is necessary to carry out pairwise alignment, which allows revealing conservative residues in the whole family or separate subfamilies of proteins. (custom-essay.org)
Specific residues of influenza A virus (IAV) PB1-F2 proteins may enhance inflammation or cytotoxicity. (cdc.gov)

You will also learn strategies for improving the production of "difficult" proteins. (lu.se)
Strategies for improving production of "difficult" proteins will be presented. (lu.se)

Affinity tagging, a popular method for capturing or immobilizing proteins, has several incarnations, none more popular than polyhistidine tagging. (genengnews.com)
After expression, the protein is captured by means of immobilized metal affinity chromatography (IMAC). (genengnews.com)

Hemagglutinin with reduced glycan occupancy required a higher concentration of surfactant protein D (an important innate immunity respiratory tract collectin) for inhibition compared to that with normal glycan occupancy. (cdc.gov)

When studying protein function, researchers must often express a protein of interest in different systems for different applications. (the-scientist.com)

Protein glycosylation fulfils a variety of roles. (iucr.org)

production of perdueterated proteins for neutron-scattering experiments. (lu.se)

Particular importance here is the biological applications of Pt NCs such as the bioimaging of various cells as a preferred fluorophore in contrast to traditional fluorescent markers (e.g., organic dye, semiconductor quantum dots, and fluorescent proteins), the usage of Pt NCs-based antitumour drugs as a new class chemotherapeutics for malignant tumour therapy, and the utilization of antibacteria as an alternative of Ag-based antibacterial agent. (hindawi.com)

In addition, the capsule prevents bacterial death caused by bactericidal serum factors. (medscape.com)

Chimerigen Laboratories develops and manufactures high quality and leading edge proteins for biomedical and immunology research. (bio-connect.nl)

Dr. Liu's research group primarily employs X-ray crystallography in combination with mechanistic, biological and computational methods to study proteins involved in bacterial infections and other human diseases. (luc.edu)
This process allows a variety of important applications, including the study of gene regulation, the analysis of the expression of proteins within eukarotic cells such as those of mammals, the production of transgenic organisms , and the methods of gene therapy. (newworldencyclopedia.org)
On the other hand, if it is not possible to find a template for modelling protein structure, then physical and chemical methods are used. (custom-essay.org)

Massive-scale transient expression in Chinese language Hamster Ovary (CHO) cells offers a fast protein manufacturing methodology with a possible start-to-end alignment benefit for biotherapeutics drug discovery. (molvisindex.org)
Lipopolysaccharide-binding protein facilitates transfer of bacterial cell wall components to inflammatory cells. (medscape.com)
It is also possible to capture the entire population of proteins in cells that bind to metals such as copper or iron, the so-called metalloproteome," adds Dr. Boronowsky. (genengnews.com)
Pneumococcal adhesion and infection of A549, BEAS-2B, and primary human bronchial airway cells were assessed by means of quantitative bacterial culture and expressed as colony-forming units (CFU). (cdc.gov)
In A549 and BEAS-2B cells MS-WF increased pneumococcal adhesion and infection and PAFR protein expression. (cdc.gov)
2019) Cartilage Oligomeric Matrix Protein initiates cancer stem cells through activation of Jagged1-Notch3 signaling. (lu.se)
Cells treated with NGI-1 produced morphologically unaltered viable influenza virus with sequence-neutral glycosylation changes (primarily reduced site occupancy) in the hemagglutinin and neuraminidase proteins. (cdc.gov)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

As a result, the reprogrammed strain (designated EcNe) enabled efficient expression of HlyE in a temporal control manner. (nature.com)
2017 ) Right time, right place: the temporal regulation of developmental gene expression. (academictree.org)

On Target 2, on-going studies aim to inhibit acylase to disrupt bacterial iron acquisition. (luc.edu)

In addition, Alaish noticed that animals with such leaky walls had abnormal bacterial content in their guts. (hopkinsmedicine.org)
For this reason, specialists are interested in anticipating a protein abnormal to the host organism in the carrier bacterium so that a drug can be developed in time. (custom-essay.org)

Bacterial densities measured by fluorescence yielded more consistent data between technical replicates than optical density measurements. (biorxiv.org)

RNA codons and protein synthesis. (crossref.org)
SECIS-binding protein 2, a key player in selenoprotein synthesis, is an intrinsically disordered protein. (igbmc.fr)
Protein synthesis, folding. (msdmanuals.com)

Anatomy24

Organisms30

Diseases5

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes78

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science5

Health Care1

Strains7

Mammalian5

Complement proteins4

Plasmid1

Perdeuterated proteins for neutron-scattering1

Purification4

Effector proteins4

Antibodies3

Bacteria10

Antigens1

Plasmids1

Electrophoresis2

Enzymes3

Genome5

Virulence3

Intracellular2

Membrane5

Escherichia3

Species3

Genes2

Genetic4

Pathogenicity2

Fusion proteins3

Populations3

Interactions3

Functional3

Biochemical3

Molecular3

Molecules1

Growth6

Lund Protein Production Platform1

Nucleic1

Intestinal3

Immune1

Purify2

Genomic1

Carbohydrates1

Putative1

Therapeutic1

Phylogenetic1

Residues2

Strategies2

Affinity2

Glycan1

Researchers1

Roles1

Experiments1

Fluorescent1

Serum1

Immunology1

Methods3

Cells8

Temporal2

Inhibit1

Abnormal2

Fluorescence1

Synthesis3