• Outer membrane porins in Gram-negative bacteria facilitate antibiotic influx. (imperial.ac.uk)
  • During her postdoctoral studies at the University of Massachusetts Medical School (UMASS), she focused on the structure and function of outer membrane proteins, mainly Occ-family of porins from P. aeruginosa , and their role in bacterial pathogenesis and antibiotic resistance. (nih.gov)
  • The pathway apparatus comprises three components: two within the inner membrane and one within the outer. (wikipedia.org)
  • An FHIPEP protein is located within the inner membrane, although it is unknown which component it constitutes. (wikipedia.org)
  • The inner membrane is permeable to what? (flashcardmachine.com)
  • The innermost compartment in chloroplasts, which is formed by invaginations of the chloroplast's inner membrane. (flashcardmachine.com)
  • Gram-negative bacteria are surrounded by both an inner membrane (IM) and an outer membrane (OM). (genengnews.com)
  • MlaC probably accepts a phospholipid from MlaA and shuttles it to the ABC transporter for ATP-dependent plasma membrane insertion 5 . (nature.com)
  • The structure and spontaneous curvature of clathrin lattices at the plasma membrane. (nih.gov)
  • Viruses that infect eukaryotic cells typically bind to a plasma membrane receptor to initiate the reproduction cycle. (virology.ws)
  • Bacterial flagella are entirely outside the cell membrane (plasma membrane) and are normally visible only with the aid of an electron microscope . (newworldencyclopedia.org)
  • It is a common pathway amongst Gram-negative bacteria for secreting toxic and flagellar proteins. (wikipedia.org)
  • This asymmetry is maintained by the Mla pathway, a six-component system that is widespread in Gram-negative bacteria and is thought to mediate retrograde transport of misplaced phospholipids from the outer leaflet of the OM to the cytoplasmic membrane 4 . (nature.com)
  • The two-partner secretion pathway in Gram-negative bacteria consists of a TpsA exoprotein and a cognate TpsB outer membrane translocator protein. (duke.edu)
  • Limulus Amebocyte Lysate (LAL) tests detect and quantify bacterial endotoxins extracted from the outer membrane of gram negative bacteria. (acciusa.com)
  • Endotoxin, or lipopolysaccharide (LPS), is a constituent of the outer leaflet (OL) of the outer membrane (OM) of gram-negative bacteria. (europeanpharmaceuticalreview.com)
  • It is a unique molecule used as a marker of prokaryotic invasion by metazoan immune systems and occurs as a singular constituent among multiple gram-negative bacteria OM constituents, including phospholipids and surface proteins. (europeanpharmaceuticalreview.com)
  • it is the product of a bacterial manufacturing process that involves nine separate enzymatic events, followed by export to the gram-negative bacteria cell surface. (europeanpharmaceuticalreview.com)
  • Unique sugars in LPS include the core sugar, KDO (3 deoxy-α-D-manno-octulosonic acid), and unique arrangements of sugars in the O-antigen moiety, which are used to distinguish bacterial strains when characterising various gram-negative bacteria foodborne illness outbreaks (serotyping). (europeanpharmaceuticalreview.com)
  • Recently, darobactin A, a novel bicyclic heptapeptide that selectively kills Gram-negative bacteria by targeting the outer-membrane protein BamA, was discovered. (sdsc.edu)
  • OM asymmetry, or more precisely the LPS in the outer leaflet, is the basis for the high intrinsic resistance of Gram-negative bacteria toward antibiotics and other toxic compounds," van den Berg explained to GEN . "This is due to the very special properties of LPS that are unique to Gram-negative bacteria. (genengnews.com)
  • Attempts to generate novel antibiotics to address this problem often fail because the outer membrane (OM) of gram-negative bacteria acts as a barrier that blocks the entry of many small molecules. (hhs.gov)
  • gram-negative bacteria are protected by lipopolysaccharides in their outer membrane. (msdmanuals.com)
  • Antibiotics are 'magic bullets' with the aim of targeting parts of the bacterial physiology that are not present in eukaryotic cells. (futurelearn.com)
  • I attended a Systems biology meeting ESF 2008 where Ron Weiss explained how he used a bacterial to manipulate eukaryotic cells. (igem.org)
  • CT load was based on the copy number of the major outer-membrane protein (MOMP) gene normalised per copy number of eukaryotic cells (HLA gene). (bmj.com)
  • However, previous work by this team of researchers from the University of Notre Dame and The University of Chicago has shown that, contrary to popular belief, intrinsically disordered proteins, which sample a variety of conformational forms the way the autotransporter proteins do, can remain quite extended in water even if they have high ratios of hydrophobic to charged amino acids. (anl.gov)
  • Depending on the degree of dehydration, dry mucous membranes, hypotension, prolonged capillary refill time, and poor skin turgor may be present. (medscape.com)
  • Many mucous membranes are bathed in secretions that have antimicrobial properties. (msdmanuals.com)
  • While the IM is formed by a symmetric phospholipid (PL) bilayer, the OM is structured as an asymmetric bilayer, comprising an inner leaflet of PLs and an outer leaflet that is composed of lipopolysaccharides (LPSs). (genengnews.com)
  • Mycobacteria outer membrane, rich in lipids and essential disaccharide precursors such as trehalose, has been the prime target for drug development against tuberculosis. (uml.edu)
  • The antiphospholipid (aPL) autoantibodies bind moieties on negatively charged PLs or moieties formed by the interaction of negatively charged PLs with other lipids, PLs, or proteins. (medscape.com)
  • In molecular biology, the FHIPEP protein family (Flagellar/Hr/Invasion Proteins Export Pore family)consists of a number of proteins that constitute the type III secretion (or signal peptide-independent) pathway apparatus. (wikipedia.org)
  • Here we show, using X-ray crystallography, molecular dynamics simulations and in vitro and in vivo functional assays, that MlaA is a monomeric α-helical OM protein that functions as a phospholipid translocation channel, forming a ~20-Å-thick doughnut embedded in the inner leaflet of the OM with a central, amphipathic pore. (nature.com)
  • This architecture prevents access of inner leaflet phospholipids to the pore, but allows outer leaflet phospholipids to bind to a pronounced ridge surrounding the channel, followed by diffusion towards the periplasmic space. (nature.com)
  • Previous work has demonstrated that the TpsB protein forms a beta-barrel structure with pore forming activity and facilitates translocation of the TpsA protein across the outer membrane. (duke.edu)
  • Functional and biochemical analysis of the c-Myc epitope tag insertions and a series of HMW1B deletion constructs demonstrated that the periplasmic domain is required for secretion of HMW1 and that the C-terminal membrane anchor (HMW1B-(234-545)) is capable of oligomerization and pore formation. (duke.edu)
  • Similar to our observations with HMW1B, examination of a Bordetella pertussis TpsB protein called FhaC revealed that the C terminus of FhaC (FhaC-(232-585)) is capable of pore formation. (duke.edu)
  • We speculate that all TpsB proteins have a modular structure, with a periplasmic domain that interacts with the cognate TpsA protein and with pore forming activity contained within the C terminus. (duke.edu)
  • Effectively, the Mla protein is structured like a donut, sited in the inner leaflet of the OM, which acts like a one-way channel, allowing removal of PLs from the outer OM leaflet, but preventing inner-leaflet PLs from entering the pore. (genengnews.com)
  • This work provides a conceptual framework for how microbes use a fluid outer membrane receptor to recognize and assemble kin cells into a cooperative multicellular community that resembles a tissue. (nature.com)
  • Molecular mechanics underlying flat-to-round membrane budding in live secretory cells. (nih.gov)
  • Attachment of bacteriophages to bacterial cells is more diverse. (virology.ws)
  • This method is expected to facilitate the detection of receptors expressed in other bacterial species or human cells by simply changing the ligand. (uml.edu)
  • They do this by either collapsing proteins in bacterial cells or damaging the outer membrane of viruses and similar pathogens. (purewatergazette.net)
  • Bismuth and cefiderocol showed synergistic effect both in vitro and in vivo (lung infectious model) against PAO1, a strain of Pseudomonas aeruginosa, as a metallo-sideromycin complex transported actively into bacterial cells. (hku.hk)
  • This approach allows these antibiotics to be delivered into bacterial cells through a pathway that mimics iron uptake. (hku.hk)
  • One such function is tissue-specific bacterial engulfment in human cells which do not normally undergo phagocytosis. (umich.edu)
  • States have been and continue to be prepared from suspensions of inactivated or disrupted Bordetella pertussis whole bacterial cells. (cdc.gov)
  • This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with activation of the complement system and a range of antimicrobial peptides, such as defensins, cathelicidin and saposins. (bvsalud.org)
  • Local secretions also contain immunoglobulins, principally IgG and secretory IgA, which prevent microorganisms from attaching to host cells, and proteins that bind iron, which is essential for many microorganisms. (msdmanuals.com)
  • For example, the high resistance of P. aeruginosa against conventional antibiotics can be attributed in part to the limited permeability of the outer membrane (OM) and the expression of 'efflux pump', specialised proteins within bacteria that actively remove antibiotics, thus reducing their effectiveness. (hku.hk)
  • The fluoroquinolone susceptibility profile for each clinical isolate is determined by the number and location of mutational changes in specific enzyme target sites, porin proteins, and efflux mechanisms. (cdc.gov)
  • Structural Insights into the Mechanism of Dynamin Superfamily Proteins. (nih.gov)
  • He also made significant contributions to computational structural biology, particularly in the analysis of protein-protein interfaces. (psi.ch)
  • We are interested in protein structure both from a structure determination and from a structural bioinformatics perspective, which means obtaining high resolution "bioimages" of proteins and extracting as much information as possible from them. (psi.ch)
  • Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that catalyze post-translational deimination/citrullination of proteins, causing structural and functional changes in target proteins. (open.ac.uk)
  • How does an amino acid sequence that is unfolded when it leaves the ribosome manage to fold properly into a highly ordered, lightning-fast enzyme or sturdy structural protein? (anl.gov)
  • In 2015, she joined the Laboratory of Structural Biology Research and the Protein Expression Laboratory at the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). (nih.gov)
  • Dr. Eren's research focuses on understanding the structural and mechanistic aspects of Rev-host protein interactions and finding target regions to inhibit Rev activity using antibodies, synthetic peptides, and aptamers. (nih.gov)
  • The researchers report on studies investigating the structure and function of the Mla protein, in Nature Microbiology , in a paper entitled " Structural Basis for Maintenance of Bacterial Outer Membrane Lipid Asymmetry . (genengnews.com)
  • Mutations that result in alterations of the outer membrane porin proteins of Gram-negative organisms lead to decreased permeability of the drug through the outer membrane so less drug reaches the target enzyme. (cdc.gov)
  • alters an outer membrane protein which prevents the drug getting into the bacterial cell and accessing its intracellular target. (futurelearn.com)
  • Notable structures include thioredoxin structures, glutamate decarboxylase, and bacterial type 1 pili. (psi.ch)
  • Some attach to bacterial outer membrane proteins, while others attach to appendages such as pili or flagella. (virology.ws)
  • This mechanism translocates proteins lacking an N-terminal signal peptide across the cell membrane in one step, as it does not require an intermediate periplasmic process to cleave the signal peptide. (wikipedia.org)
  • This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. (sigmaaldrich.com)
  • During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. (sigmaaldrich.com)
  • Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e. (cdc.gov)
  • The _____ membrane encloses the stroma, a concentrated solution of ______, including those required for ______ synthesis. (flashcardmachine.com)
  • The stroma also contains the ___,____, and ribosomes involved in the synthesis of several chloroplast proteins. (flashcardmachine.com)
  • 2022 ) Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae . (lu.se)
  • Gram-negative bacterial infections, such as those caused by Pseudomonas aeruginosa , pose significant challenges in treatment due to their complicated structure. (hku.hk)
  • The Scholars of the Podcast reveal ribosomal proteins encoded in viral genomes, and a protein cell receptor for bat influenza viruses. (virology.ws)
  • A trehalose-functionalized photoaffinity nanoprobe that could photo-crosslink the protein receptor on mycobacteria was developed to investigate these specific interactions further. (uml.edu)
  • The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli . (bionity.com)
  • Scholars@Duke publication: Translocator proteins in the two-partner secretion family have multiple domains. (duke.edu)
  • These proteins are synthesized in the bacterial cytoplasm and cross one membrane into the bacterial periplasm. (anl.gov)
  • Evidence for this reconnaissance emerged in 2009, when University of California, Davis, plant pathologist Pamela Ronald discovered a bacterial protein called Ax21 in some strains of rice. (discovermagazine.com)
  • Scientists at the U.K.'s University of Newcastle suggest that a bacterial membrane protein that acts as a type of membrane vacuum cleaner could represent a promising new target for rendering harmful Gram-negative strains susceptible to existing as well as new antibiotics. (genengnews.com)
  • Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
  • The enzymatic cascade used to "build" LPS, as encoded in the bacterial genome, is not shown but is extensively detailed by Wang and Quinn and supports that LPS is a specific functional unit. (europeanpharmaceuticalreview.com)
  • The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome . (bionity.com)
  • However, deletion of a phage protein (p3) prevents full escape from the host E. coli , and phage that are 10-20X the normal length with several copies of the phage genome can be seen shedding from the E. coli host. (bionity.com)
  • These genomes reveal diverse mobile elements, including an integrative viral genome that bidirectionally replicates in a circular form and aloposons, transposons that encode the 5,000 amino acid-sized proteins Otus and Ephialtes. (darkenergybiosphere.org)
  • Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. (bmj.com)
  • In some bacterial species, the flagella twine together helically outside the cell body to form a bundle large enough to be visible in a light microscope . (newworldencyclopedia.org)
  • The β-lactam family of drugs target the bacterial cell wall and therefore, would not have any effect on Mycoplasma species. (futurelearn.com)
  • Our group is interested in the research of clinically relevant bacterial species that mainly cause upper and lower airway infections and their interactions with the host. (lu.se)
  • RNA codon misreading [formation of toxic, non functional proteins]. (mindmeister.com)
  • In this study, we characterized the functional domains of the Haemophilus influenzae HMW1B protein, a TpsB protein that interacts with the H. influenzae HMW1 adhesin. (duke.edu)
  • This has important functional implications because it would result in a locally greater pH gradient across cristal membranes than across inner membranes that are not part of cristae, thereby significantly influencing the rate of oxidative phosphorylation (Section 18-3). (flashcardmachine.com)
  • The researchers carried out X-ray crystallography to elucidate the 3D structure of MlaA, combined with computation and functional assays to identify important residues in the protein. (genengnews.com)
  • Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
  • Maintaining dosing is important for some antibiotics in minimising the selection of antibiotic resistant mutants in high density bacterial populations. (futurelearn.com)
  • Antibiotic resistance typically induces a fitness cost that shapes the fate of antibiotic-resistant bacterial populations. (elifesciences.org)
  • Such strategies implicitly presume that resistance leads to reduced bacterial fitness in an antibiotic-free environment, and therefore these resistant populations should be rapidly outcompeted by antibiotic-sensitive variants. (elifesciences.org)
  • These factors collectively impede the accumulation of antibiotics at the bacterial target site. (hku.hk)
  • Blocking the protein, which is part of the Mla (maintenance of outer membrane lipid asymmetry) system, would effectively disrupt the composition of one of the two layers of the bacterium's outer membrane, which normally acts as a barrier to toxic chemicals, such as antibiotics. (genengnews.com)
  • We are studying protein structures and crystal lattices by employing a variety of approaches, in particular concepts from the field of molecular evolution. (psi.ch)
  • Molecular chaperones play a key role in cellular processes, including protein homeostasis, but also in membrane protein transport and biogenesis. (unibas.ch)
  • Recently, we have systematically investigated how molecular chaperones interact with the intrinsically disordered protein α-synuclein. (unibas.ch)
  • Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis. (bmj.com)
  • These proteins include dynein, a molecular motor that can cause flagella to bend, and propel the cell relative to its environment or propel water or mucus relative to the cell. (newworldencyclopedia.org)
  • Hijacking pathogenic membrane proteins to engineer cellular entry: A molecular biophysics approach Invasive pathogenic bacteria feature many cellular niches and life cycles, for which they have developed functions that are potentially attractive in biotechnology and therapeutic delivery applications. (umich.edu)
  • There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
  • Outer membrane and membrane vesicles (OMV/MV) are released from bacteria and participate in cell communication, biofilm formation and host-pathogen interactions. (open.ac.uk)
  • Here we show phylogenetically conserved pathways of PAD-mediated OMV/MV release in bacteria and describe deiminated/citrullinated proteins in E. coli and their derived OMV/MVs. (open.ac.uk)
  • The system involves the study of what are termed autotransporter proteins, which pathogenic bacteria secrete as virulence factors for infection. (anl.gov)
  • Using c-Myc epitope tag insertions and cysteine substitution mutagenesis, we discovered that HMW1B contains an N-terminal surface-localized domain, an internal periplasmic domain, and a C-terminal membrane anchor. (duke.edu)
  • Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
  • The objective of this study was to compare the CT load between 2 cohorts of CT positive patients (1) those attending a sexually transmitted infection (STI)-clinic and (2) those participating in the Dutch population-based screening (CSI), thereby taking into account symptoms as well as other determinants relevant for bacterial load. (bmj.com)
  • These form the rounded tip of the phage and are the first proteins to interact with the E. coli host during infection. (bionity.com)
  • Periodontal disease is a chronic bacterial infection that affects the gingiva and bone that supports the teeth. (bvsalud.org)
  • MlaA represents a lipid transport protein that selectively removes outer leaflet phospholipids to help maintain the essential barrier function of the bacterial OM. (nature.com)
  • The Gram-negative bacterial outer membrane (OM) is a unique bilayer that forms an efficient permeation barrier to protect the cell from noxious compounds 1 , 2 . (nature.com)
  • Fundamentals of Bacteriology will provide you with a detailed understanding of bacterial cell structure including a particular focus on the cell surface. (manchester.ac.uk)
  • This unit will provide students with a comprehensive overview of bacterial cell structure/function with a particular focus on the key cell surface components. (manchester.ac.uk)
  • A flagellum (plural, flagella ) is a long, whip-like projection or appendage of a cell composed of microtubules (long, slender, protein tubes) and used in motility. (newworldencyclopedia.org)
  • The flagellum of eukaryotes usually moves with an "S" motion and is surrounded by cell membrane . (newworldencyclopedia.org)
  • This incorporation enables the active transport of the antibiotic into bacterial cell through nutrient pathways. (hku.hk)
  • Also, all applications where a bacterial cell will be used within a eukaryotic organism (medical applications) are far from being ethically approved. (igem.org)
  • The "molecule" could for instance be a cancer cell or a viral protein. (igem.org)
  • Why don't all the proteins in the cell instead just stick to each other, aggregating into a big mess? (anl.gov)
  • This is thought to make globular proteins stable and soluble in the watery environment of the cell. (anl.gov)
  • For this work, they turned to the N-terminal passenger domain of the pertactin virulence protein from Bordetella pertussis, a typical autotransporter. (anl.gov)
  • Bradley D, Viéitez C, Rajeeve V, Selkrig J , Cutillas PR & Beltrao P (2021) Sequence and Structure-Based Analysis of Specificity Determinants in Eukaryotic Protein Kinases. (ukaachen.de)
  • It is possible that members of the FHIPEP family serve as pores for the export of specific proteins. (wikipedia.org)
  • Filamentous phage use a bacterial structure known as the F pilus to infect E. coli , with the M13 p3 tip contacting the TolA protein on the bacterial pilus. (bionity.com)
  • This plasmid is designed to express tagged proteins in E. coli. (sigmaaldrich.com)
  • Unexpectedly, MlaA is an alpha-helical integral membrane protein (not a humble lipoprotein as predicted), mostly located in the inner leaflet of the OM but partly inserted into the outer leaflet," writes lead co-research and lead author, Javier Abellon-Ruiz, in an accompanying blog . (genengnews.com)
  • Antimicrobial resistance (AMR) in bacterial infections has emerged as a significant global health concern. (hku.hk)
  • President & CEO: Isao Teshirogi, Ph.D.) (hereafter "Shionogi") today announces the back to back publication of two studies in The Lancet Infectious Diseases journal highlighting the efficacy and safety of cefiderocol against some of the most difficult-to-treat Gram-negative bacterial infections, including nosocomial pneumonia (NP), bloodstream infections (BSI), sepsis, and complicated urinary tract infections (cUTI). (dutchnews.nl)
  • The rising frequency of drug-resistant bacterial infections pose an imminent threat to human health. (hhs.gov)
  • RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
  • She currently studies the underlying mechanisms of viral pathogenesis, viral protein-host protein interactions, and the potential use of humanized scFvs and nanobodies for treatment. (nih.gov)
  • The most potent compound, darobactin B, was used for co-crystallization with the target BamA, revealing an identical binding site to darobactin A. Besides its potency, darobactin B did not exhibit cytotoxicity and was slightly more active against Acinetobacter baumanii isolates than darobactin A. Furthermore, we evaluated the plasma protein binding of darobactin A and B, indicating their different pharmacokinetic properties. (sdsc.edu)
  • They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. (bmj.com)
  • There is great need for therapeutics against multi-drug resistant, Gram-negative bacterial pathogens. (sdsc.edu)
  • Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)
  • PapB/FocB family proteins are small DNA-binding transcriptional regulators consisting of approximately 100 amino acids that have been reported to regulate the expression of various fimbriae, including P, F1C, and type 1 fimbriae, and adhesins. (bvsalud.org)
  • So, if you are a Master's student looking for a challenging but also rewarding research project, who is interested in bacterial genetics, we encourage you to apply to our project. (lu.se)
  • Three systems are known to maintain OM lipid asymmetry: the OM phospholipase A 2 PldA 7 , the lipopolysaccharide (LPS) palmitoyl transferase PagP 8 and the Mla (maintenance of outer membrane lipid asymmetry) system. (nature.com)
  • Cryo-EM of the dynamin polymer assembled on lipid membrane. (nih.gov)
  • Interestingly there is one additional phage-encoded protein, pX (p10), that is important for regulating the number of double stranded genomes in the bacterial host. (bionity.com)
  • Eukaryotic genomes are known to have garnered innovations from both archaeal and bacterial domains but the sequence of events that led to the complex gene repertoire of eukaryotes is largely unresolved. (darkenergybiosphere.org)
  • Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. (bmj.com)
  • Results Our 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. (bmj.com)
  • To date, only one bacterial factor has been shown to mediate intestinal adherence by O157:H7, the outer membrane protein intimin which was originally discovered in the Principal Investigator's laboratory. (usda.gov)
  • Tong J, Dolezal P, Selkrig J , Crawford S, Simpson AGB, Noinaj N, Buchanan SK, Gabriel K & Lithgow T (2011) Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana. (ukaachen.de)
  • The HIV-1 Rev protein is a key regulatory factor that is essential for both early and late phases of viral replication cycles, and therefore represents an important viral target for drug development. (nih.gov)
  • Insertion of proteins into the bacterial outer membrane is mediated by a dedicated chaperone machinery. (unibas.ch)
  • The mere presence of an immunity-inducing protein like Ax21 was not that unusual-the immune systems of most organisms identify a microbial intruder through proteins protruding from its outer membrane. (discovermagazine.com)
  • After her postdoctoral studies, Dr. Eren joined the National Institute of Allergy and Infectious Diseases (NIAID) at NIH as a research fellow where she studied host immune system manipulation by Rotavirus proteins. (nih.gov)
  • Design We first developed and validated a workflow-including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS-to discover protein signals from CD-associated gut microbial communities. (bmj.com)
  • MlaA is the most enigmatic Mla component, particularly since its identity as a periplasmically exposed lipoprotein appears hard to reconcile with its proposed activity on outer leaflet phospholipids. (nature.com)
  • The Newcastle team focused on the MlaA protein, which is the OM-sited component of the six-protein Mla system. (genengnews.com)
  • The most surprising insight was that MlaA is mostly embedded in the OM, which was not at all predicted," van den Berg further commented to GEN . "MlaA has also a structure (alpha-helical) that is common in other membrane proteins but very rare in OM proteins. (genengnews.com)
  • Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature. (nih.gov)
  • This dictum was shattered by the discovery of giant viruses which were found to encode tRNAs, aminoacyl tRNA syntheses, and many proteins involved in translation. (virology.ws)
  • Dr. Eren received her Ph.D. from Worcester Polytechnic Institute (WPI), where she studied metal transport mechanisms across membranes by P-type ATPases. (nih.gov)
  • FHIPEP proteins have all about 700 amino acid residues. (wikipedia.org)
  • A redox-active protein that carries electrons via a prosthetic Fe-containing heme group. (flashcardmachine.com)
  • 2012). This approach (EPPIC, Evolutionary Protein Protein Interface Classifier) is available to the community as an open source command-line tool and as a web server ( www.eppic-web.org (link is external) ), linked to a database (EPPIC db) that contains precomputed analysis results (Baskaran et al. (psi.ch)