Bacterial gene targeting vectors. Medical search. Frequent questions

For example, a bacterial infection may be reversed by penicillin, which can be seen as an information vector that disrupts the bacterial wall, thus killing the bacteria and reversing the disease state. (senescence.info)
Although they can be found in archaea and eukaryotes, they play the most significant biological role in bacteria where they can be passed from one bacterium to another by a type of horizontal gene transfer (conjugation), usually providing a benefit to the host, such as antibiotic resistance. (addgene.org)
This invention introduces novel methods and compositions for the preparation of a genomic library, specifically a mouse genomic library, for producing knock-out targeting vectors by arraying into pools bacteria transformed with the yeast-Escherichia coli shuttle vector comprising mouse genomic DNA fragments. (cellmedicine.com)
Bacteria present an attractive class of vector for cancer therapy, possessing a natural ability to grow preferentially within tumours following systemic administration. (escholarship.org)
Bioluminescent Imaging (BLI) represents a powerful tool for use with bacteria engineered to express reporter genes such as lux. (escholarship.org)
Bacterial lux signal was detected specifically in tumours of mice post i.v.-administration and bioluminescence correlated with the numbers of bacteria recovered from tissue. (escholarship.org)
biodegradable enzymes present in the genes of some bacteria degrade HCH in soil. (ispub.com)
Bioinformatics strategies were used to find other homologous genes and proteins, which can also degrade HCH present in various bacteria for controlling of pollution and offer clue to prevent its entry in the food chain. (ispub.com)
PCR using the new primer pair on a set of template DNAs of different bacterial genera showed that the method was selective for bacteria belonging to the family Sphingomonadaceae . (ispub.com)
Recent advances in bacterial cancer therapies suggest this idea is a new one but in fact using bacteria as a potential therapy for cancers dates back to the late 1800s and very early 1900s where gangrenous patients were observed to cure themselves of tumours as did patients suffering from what was once called acute onset cellulitis (now known as erysipelas or St Anthony's Fire). (scientificamerican.com)
This study also identified 43 growth-impairing inserts carrying multiple loci targeting 105 genes, of which 25 have homologs reported as essential in other bacteria. (biomedcentral.com)
Mobile group II introns ("targetrons") expressed through an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon is used for efficient gene targeting in a variety of gram-negative bacteria. (readabstracts.com)
This broad-host-range system for targetron expression might facilitate gene targeting in a wide range of bacteria. (readabstracts.com)
Until recently, CRISPR/Cas9-based gene editing methods 5 have been overlooked as a microbial gene editing tool because most bacteria lack endogenous DSB repair systems, rendering Cas9 nuclease cleavage toxic. (sigmaaldrich.com)
(B) Vector Map of CRISPR HR gRNA Plasmids for Bacteria. (sigmaaldrich.com)

samples that were PCR positive for Bartonella DNA important bacterial pathogens in veterinary and human by the ITS primers were subsequently analyzed by a second medicine. (cdc.gov)
phytoplasmas are unculturable bacterial plant pathogens transmitted by phloem-feeding hemipteran insects. (liverpool.ac.uk)
Targeting of novel essential pathways is expected to play an important role in the discovery of new antibacterial agents against bacterial pathogens, such as Pseudomonas aeruginosa , that are difficult to control because of their ability to develop resistance, often multiple, to all current classes of clinical antibiotics. (biomedcentral.com)
This study aimed to rapidly detect bacterial, fungal and parasitic pathogens in the guts of A. agrarius using NGS-based metabarcoding analysis. (biomedcentral.com)
Herpesvirus of turkeys (HVT), used originally as a vaccine against Marek's disease (MD), has recently been shown to be a highly effective viral vector for generation of recombinant vaccines that deliver protective antigens of other avian pathogens. (mdpi.com)
The mRNA vaccines can encode multiple antigens, strengthening the immune response against pathogens and enabling the targeting of multiple microbial variants [19] . (researchgate.net)
These pathogens have animal reservoirs as vectors for transmission. (bvsalud.org)
Proper investigation of the pathogens in their rodent vectors could help reduce and manage their emergence and spread. (bvsalud.org)

Over the next 100 years, many significant discoveries lead to the conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. (addgene.org)
Alphaviral envelope pseudotypes of retroviruses or lentiviruses are able to integrate the genes that they carry into the expansive range of potential host cells that are recognized and infected by the alphaviral envelope proteins E2 and E1. (genetherapynet.com)
Furthermore, the use of whole alphaviruses for gene therapy is of limited efficacy both because several internal alphaviral proteins are involved in the induction of apoptosis upon infection and also because the alphaviral capsid mediates only the transient introduction of mRNA into host cells. (genetherapynet.com)
This system utilizes the interaction between LacI (repressor) and LacO (operator) proteins, derived from the bacterial lactose operon to regulate shRNA expression in the presence and absence of the lactose analogue, isopropyl-ß-D-thiogalactoside (IPTG). (vectorbuilder.kr)
Title: Differential gene expression and immunolocalization of insulin-like growth factors and insulin-like growth factor binding proteins between experimental nonunions and standard healing fractures. (genscript.com)
The initial choice of this vector was based upon our experience in the successful expression and production of thirty soluble and membrane associated proteins, many of which contained [2Fe-2S] clusters or flavin cofactors. (secsg.org)
To express proteins, one liter cultures of E. coli containing the desired cDNA in the expression vector are grown in Circlegrow (Qbiogene) medium at 30 deg. (secsg.org)
Towards this end we have actively examined new, more effective expression vectors for production of human proteins. (secsg.org)
We found those vectors currently available for bacterial expression to be less, or no more, effective for difficult proteins than our current Tac-based expression vector. (secsg.org)
However, we have recently produced a new expression vector that is based upon the Tac promoter but is a fusion with a newly-identified human protein that has given significantly increased yields for a number of test proteins. (secsg.org)
This system has great potential to produce properly processed mammalian proteins in significant yield if we can successfully design a convenient vector system for cassette insertion of cDNAs of interest. (secsg.org)

A total of 58 samples along the entire digestive tract were analyzed for microbial community using 16S rRNA gene sequencing. (bvsalud.org)
classification of new phytoplasmas associated with diseases of strawberry in florida, based on analysis of 16s rrna and ribosomal protein gene operon sequences. (liverpool.ac.uk)
analysis of 16s rrna gene sequences amplified by pcr indicated that the symptoms were associated with infection by a phytoplasma, designated strawberry yellows (strawy) phytoplasma. (liverpool.ac.uk)
Ticks were examined for Ehrlichia and Anaplasma by nested PCR directed at the gltA, antigen-expressing gene containing a variable number of tandem repeats, 16S rRNA, and groESL genes. (cdc.gov)
Amplicon-based next-generation sequencing (NGS) targeting the 16S ribosomal RNA (rRNA) gene is the most common technique used to analyze the bacterial microbiome. (biomedcentral.com)
The 16S rRNA gene analysis showed that the microbial composition of the cecal samples changed by season ( P = 0.005), with the linear discriminant analysis effect size showing that in the spring Escherichia coli and Lactobacillus murinus were more abundant and Helicobacter rodentium was less abundant. (biomedcentral.com)
16S rRNA gene data derived from a shotgun metagenome of the same reactor sample added an additional perspective on the community structure. (frontiersin.org)
Furthermore, in-silico evaluation of primers, especially those for amplification of archaeal 16S rRNA gene regions, does not necessarily reflect the results obtained in experimental approaches. (frontiersin.org)
Overall our results indicate a rather limited comparability between community structures investigated and determined using different primer pairs as well as between metagenome and 16S rRNA gene amplicon based community structure analysis. (frontiersin.org)
The by far most often used marker for prokaryotic diversity studies is the 16S rRNA or its corresponding gene. (frontiersin.org)

Further disclosed are the procedures for preparing a genomic library for use in producing knockout targeting vectors, including details of genomic DNA fragments, bacterial selection markers, yeast replication, and the amplification of shuttle vectors. (cellmedicine.com)
The present invention is directed to methods for producing gene targeting constructs by homologous recombination using mouse genomic libraries arrayed in yeast shuttle vectors. (cellmedicine.com)
In Cas9 nuclease-mediated genome editing, we constructed an all-in-one vector expressing Cas9 nuclease and seven gRNAs and targeted seven genomic loci. (nature.com)
Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. (frontiersin.org)
This highly sensitive phenotypic readout of enhancer function in a native genomic context reveals novel features of CRM function undetected by traditional reporter gene analysis. (elifesciences.org)
A 9.3-kb EcoRI fragment containing the first exon of the PKCe gene was isolated from a murine 129/Sv genomic DNA library. (ximbio.com)
Upon entering your gene name, you will see detailed information on all shRNAs against your GOI available in our database, including a link to UCSC Genome Browser to view these shRNAs in the context of genomic sequence and all the transcript isoforms. (vectorbuilder.kr)

primers designed from sequences of the gene encoding the elongation factor tu (tuf gene) of several culturable mollicutes amplified most of the tuf gene from phytoplasmas of the aster yellows, stolbur and x-disease groups. (liverpool.ac.uk)
Briefly, primers targeting GFP fragments in plasmid pCMV-GFP (Matsuda and Cepko 2004) or a vATPase gene fragment of D. citri [based on sequences determined in a published work (Vyas et al. (citrusrdf.org)
Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
Orthopoxviruses and Monkeypox virus were detected in the organs by PCR using consensus primers targeting the virus surface membrane haemagglutinin (HA) genes, while Leptospira species were detected by PCR using primers targeting the rrs and lfb1 genes. (bvsalud.org)

since the symptoms were suggestive of phytoplasma infection, plants were assayed for presence of phytoplasma by pcr amplification of 16s rdna and ribosomal protein (rp) gene sequences. (liverpool.ac.uk)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)
Our shRNA stem-loop sequences are optimized to mediate efficient shRNA processing and target gene knockdown. (vectorbuilder.kr)
Moreover, it says C3DNA avoids triggering an immune response, because it does not contain unintended viral or bacterial sequences. (biopharma-reporter.com)
The following Igfbp5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
This computer based process will be used to screen for 1) molecular weight, 2) pI, 3) the possession of organelle targeting motif sequences, 4) the possession of putative transmembrane segments, 5) presence of possible sites of glycosylation, and 6) possession of identifiable cofactor binding motifs. (secsg.org)
However, a disadvantage of this primer pair was its low specificity for the archaeal domain in the experimental application leading to high amounts of bacterial sequences within the dataset. (frontiersin.org)

The system is directed toward the production of gene targeting constructs by homologous recombination, and by methods generating transgenic animals. (cellmedicine.com)
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template. (sigmaaldrich.com)

To target genes in mice, the DNA is inserted into mouse embryonic stem cells in culture. (wikipedia.org)
Yang Y, Seed B. (2003 Mar) Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes . (uconn.edu)

The frequency of gene targeting can be significantly enhanced through the use of site-specific endonucleases such as zinc finger nucleases, engineered homing endonucleases, TALENS, or most commonly the CRISPR-Cas system. (wikipedia.org)
To help you unravel your genes and pathways of interest, we collaborated with The Wellcome Sanger Institute to make the first arrayed lentiviral CRISPR knockout libraries for human and mouse genomes. (sigmaaldrich.com)
Our exclusive Sanger CRISPR library provides extensive, high-quality gene knockout, so you can fast-track your research with the first whole genome arrayed library. (sigmaaldrich.com)
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. (nature.com)
To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. (nature.com)
We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
Genome editing using clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas9 is an easy and efficient strategy for the generation of gene-modified cells and organisms 1 , 2 . (nature.com)
Here, we report an easy and efficient construction system for all-in-one CRISPR/Cas9 vectors expressing Cas9 protein and up to seven gRNAs. (nature.com)
In addition, the plasmids for the second-step reaction retained the structure of a CRISPR/Cas9 expression vector harboring single gRNA and Cas9 expression cassettes and can therefore be used separately for single gene targeting. (nature.com)
In 2015, researchers reported that they had used components of the CRISPR/Cas9 genome editing system to edit genes so that they could propagate in a "Super-Mendelian" fashion. (elifesciences.org)
Schematic of Dual-Vector E. coli CRISPR λ-Red HR System. (sigmaaldrich.com)

Plasmids are fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. (addgene.org)
Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized for their use of studying and manipulating genes. (addgene.org)
Currently, two or more kinds of plasmids or DNA fragments are generally used for multiple targeting in cultured cells 7 . (nature.com)
However, cotransfection of multiple plasmids can cause low targeting efficiency in cells that are inefficient in DNA transfection. (nature.com)
In this study, we constructed plasmids to create all-in-one vectors expressing two to seven gRNAs with Cas9 nuclease or nickase ( Figure 1 ). (nature.com)
By design, our lentiviral vectors lack the genes required for viral packaging and transduction (these genes are instead carried by helper plasmids used during virus packaging). (vectorbuilder.kr)

As a result, virus produced from lentiviral vectors have the important safety feature of being replication incompetent (meaning that they can transduce target cells but cannot replicate in them). (vectorbuilder.kr)
For general information about lentiviral vectors, see our Guide to Vector Systems section on Lentiviral Expression Vectors , and for further information about this vector system please refer to the papers below. (vectorbuilder.kr)
A) Lentiviral vectors carrying IPTG-inducible U6-based scramble or EGFP-targeting shRNA expression cassettes were packaged into the corresponding lentiviral particles and transduced into HEK293T cells stably expressing EGFP. (vectorbuilder.kr)

Alphaviruses are of interest to gene therapy researchers, in particular the Ross River virus, Sindbis virus, Semliki Forest virus, and Venezuelan Equine Encephalitis virus have all been used to develop viral vectors for gene delivery. (genetherapynet.com)
The stable integration of viral genes is mediated by the retroviral interiors of these vectors. (genetherapynet.com)
They could therefore be used to vaccinate against viral, bacterial, protozoan, and tumor antigens . (genetherapynet.com)
This system is optimized for high copy number replication in E. coli, high-titer packaging of live virus, efficient viral transduction of a wide range of cells, and efficient vector integration into the host genome. (vectorbuilder.kr)
Intergalactic Therapeutics, a startup focused on non-viral gene therapy, reports positive preclinical results for its lead program, IG-002, addressing all forms of ABCA4-related retinopathies. (biopharma-reporter.com)
With these data, we have clearly demonstrated the feasibility of electro-transfer delivery of non-viral C3DNA expressing the full-length human ABCA4 gene to relevant cell types in the retina. (biopharma-reporter.com)
There are currently no approved treatments for ABCA4-related retinopathies and, while replacement with wild-type ABCA4 protein has the potential to slow or stop disease progression, standard AAV gene therapy platforms are not appropriate for addressing these diseases due to the large size of the gene and the growing safety concerns associated with viral vectors. (biopharma-reporter.com)
Intergalactic's novel platform bypasses these drawbacks and enables local delivery of non-viral gene therapy, representing a potentially transformative approach for addressing these blinding diseases," said the Cambridge, Massachusetts based firm. (biopharma-reporter.com)
C3DNA allows for design and delivery of DNA vectors beyond the size limits of viral delivery systems. (biopharma-reporter.com)
These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)

In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
Gene targeting is a biotechnological tool used to change the DNA sequence of an organism (hence it is a form of Genome Editing). (wikipedia.org)
Genetic modification usually describes the insertion of a transgene (foreign DNA, i.e. a gene from another species) into a random location within the genome. (wikipedia.org)
Gene-targeting is a specific biotechnological tool that can lead to small changes to the genome at a specific site - in which case the edits caused by gene-targeting would count as genome editing. (wikipedia.org)
Gene targeting is one specific form of genome editing tool. (wikipedia.org)
This site-specific or 'targeted' nature of genome editing is typically what makes genome-editing different to traditional 'genetic modification' which inserts a transgene at a non-specific location in the organisms' genome, as well as gene-editing making small edits to the DNA already present in the organisms, verses genetic modification insertion 'foreign' DNA from another species. (wikipedia.org)
Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. (sigmaaldrich.com)
1 Covering over 17,000 human and over 20,000 mouse genes with two optimized gRNAs for each of them, our Sanger Whole Genome Arrayed Libraries are powerful tools that can help you make the next exciting discovery. (sigmaaldrich.com)
In order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence of a few hundred or few thousand basepairs from the entire human genome. (addgene.org)
Our system provides an efficient targeting strategy for multiplex genome/epigenome editing, simultaneous activation/repression of multiple genes and beyond. (nature.com)
including the two researchers involved in the 2015 work - have used a new active genetic element called a CopyCat element and more traditional genome editing to analyze the control of a gene that coordinates the formation of a simple structure in a fruit fly - a vein in the wing. (elifesciences.org)
used genome editing to make mutations in a stretch of DNA that regulates the gene involved in wing vein formation. (elifesciences.org)
Whole-genome transposon-mutagenesis (TM) followed by identification of insertion sites is one of the most practical and frequently used experimental approaches to screen for essential bacterial genes [ 6 - 8 ]. (biomedcentral.com)

The alteration of DNA sequence in an organism can be useful in both a research context - for example to understand the biological role of a gene - and in biotechnology, for example to alter the traits of an organism (e.g. to improve crop plants). (wikipedia.org)
sequence and rflp analysis of the elongation factor tu gene used in differentiation and classification of phytoplasmas. (liverpool.ac.uk)
The protocol for inserting a gRNA-targeting sequence was the same as described in a previous paper 8 ( Figure 1 , STEP 1). (nature.com)
Oligonucleotides corresponding to each target sequence are annealed and inserted into BpiI-digested pX330A or pX330S vectors (STEP 1). (nature.com)
The 﫿rst step in generating OPTiKD hPSCs consists in obtaining oligonucleotides to be used to clone the shRNA sequence in the targeting vector. (berterolab.com)
Brie﫿y, two single-stranded oligonucleotides are annealed to form a double-stranded DNA sequence containing the shRNA sequence followed by a Pol III terminator (consisting of a stretch of four or more thymidines), as well as 5'- and 3'-end "sticky" overhangs that are complementary to those generated in the targeting vector following restriction digestion with BglII and SalI (Figures 3E and 4C). (berterolab.com)
Nucleotide sequence analysis of Rickettsia-specific 23S-5S IGS, ompA and gltA gene fragments indicated that ticks, principally A. americanum, contained novel species of Rickettsia. (cdc.gov)
This cassette contains stop codons in all three frames, an independent ribosomal entry site (IRES) followed by the LacZ gene with an SV40 polyadenylation sequence, and a neomycin phosphotransferase gene (MC1Neo poly(A). Correctly targeted GK129 ES cells were injected into C57BL/6 blastocysts. (ximbio.com)
Our Lentivirus IPTG-Inducible shRNA Knockdown vector contains a LacI sequence and a modified human U6 promoter with two repeats of the LacO element (U6/2xLacO). (vectorbuilder.kr)
The shRNA targeting the gene of interest (GOI) is placed downstream of the modified U6 promoter with an additional LacO sequence located downstream of the shRNA. (vectorbuilder.kr)

Alphavirus vectors carrying therapeutic or toxic genes used for intratumoral injections have demonstrated efficient tumor regression. (genetherapynet.com)
2021. Efficient intravenous tumor targeting using the αvβ6 integrin-selective precision virotherapy Ad5NULL-A20 . (cardiff.ac.uk)
Directly targeting therapeutic suicide gene to a solid tumor is a hopeful approach for cancer gene therapy. (biomedcentral.com)
Treatment of a solid tumor by an effective vector for a suicide gene remains a challenge. (biomedcentral.com)
Meanwhile, three human digestive system xenograft tumor models (colorectal cancer colo320, gastric cancer MKN-45 and liver cancer SSMC-7721) and a breast cancer (MDA-MB-231) model were employed to validate the universality of BF-rTK + GCV in solid tumor gene therapy. (biomedcentral.com)
Survival analysis results of multiple cancer models confirmed that BF-rTK + GCV system has a wide field of application in solid tumor gene therapy. (biomedcentral.com)
Cancer gene therapy approaches include the direct killing of tumor cells by injecting a therapeutic gene into the tumor cell or employing vaccine strategies to deliver an immunomodulatory gene that stimulates the immune system to recognize tumor antigens [ 1 ]. (biomedcentral.com)
The Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-TK + GCV) system is currently one of the best-studied tumor suicide gene therapy systems [ 9 - 11 ]. (biomedcentral.com)
NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)

Three archaeal 16S primer pairs as well as two bacterial and one general microbial 16S primer pairs were comprehensively tested by in-silico evaluation and performing an experimental analysis of a complex microbial community of a biogas reactor. (frontiersin.org)

Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
To target genes in moss, the DNA is incubated together with freshly isolated protoplasts and with polyethylene glycol. (wikipedia.org)
The Lentivirus IPTG-Inducible shRNA Knockdown vector is a highly efficient system for achieving temporal knockdown of target genes in a wide variety of mammalian cell lines and offers an effective tool for studying genes essential for cell development or cell survival. (vectorbuilder.kr)
When you design shRNA vectors on VectorBuilder's online platform, you will have the option to search for your target genes in our database. (vectorbuilder.kr)

To establish an all-in-one vector system, we modified the pX330 vector, originally developed by the Feng Zhang laboratory 4 , 8 , containing a single gRNA expression cassette and a Cas9 nuclease expression cassette. (nature.com)
The constructed vectors harboring single gRNA expression cassettes are then assembled into an all-in-one vector harboring multiple gRNA cassettes using the Golden Gate assembly method (STEP 2). (nature.com)
The 20-bp target region of the gRNA is customized for each gene target of interest. (sigmaaldrich.com)
cells transformed with a lacZ-targeting gRNA and a donor DNA template are successfully edited at the lacZ locus, knocking out function of this gene and yielding white colonies. (sigmaaldrich.com)

The combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA fragments of interest, such as genes. (addgene.org)

The relationship between gene targeting, gene editing and genetic modification is outlined in the Venn diagram below. (wikipedia.org)
In such cases the edits caused by gene-targeting would, in some jurisdictions, be considered as equivalent to Genetic Modification as insertion of foreign DNA has occurred. (wikipedia.org)
The concept of genes as carriers of phenotypic information was introduced in the early 19th century by Gregor Mendel, who later demonstrated the properties of genetic inheritance in peas. (addgene.org)
A) Schematic of the generation of OPTiKD human pluripotent stem cells (hPSCs) and of the resulting genetic circuit which allows tetracycline (TET)-dependent induction of gene knockdown both in hPSCs and hPSC-derived cells. (berterolab.com)
A Genetic Embedded Approach for Gene Selection and Classification of Microarray Data. (stanford.edu)
Therefore it is difficult to perform specific gene targeting in Xenopus tropicalis with conventional reverse genetic methods. (biomedcentral.com)

The ribosomal operons mainly 16S rDNA has proven to be a stable and specific molecular marker for bacterial identification. (ispub.com)

PCR targeting the heme-binding protein gene, Pap31. (cdc.gov)
METHODS: Ticks submitted by study participants from April-September 2011 and 2012 were identified to species and life stage, and preliminarily screened for the genus Rickettsia by nested PCR targeting the 17-kDa protein gene. (cdc.gov)
Amino Acid Features for Prediction of Protein-Protein Interface Residues with Support Vector Machines. (stanford.edu)
Protein kinase Cepsilon is required for macrophage activation and defense against bacterial infection. (ximbio.com)
The data demonstrate for the first time ever that a single subretinal administration of a DNA payload encoding the human ABCA4 gene resulted in durable - 12-month - expression of human ABCA4 protein in adult pig retinas, said the US biotech. (biopharma-reporter.com)
Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
The currently available Mammalian Gene Collection clones available through ATCC serve as the pool of candidate cDNAs for human protein production. (secsg.org)
A Nph3-KI targeting vector was designed in a way to result in the generation of a bicistron ic mRNA, encoding for a fusion Nph3-Flag protein, as well as for beta-galactosidase fused to a nuclear localization signal. (uni-goettingen.de)
1998). In parallel to the in vivo approach, a bacterial protein expression system was established, leading to the production of recombinant Nph1 and Nph3 for use in future in vitro experiments.Analysis of Nph3 expression in Nph3-KI mice was performed by assaying brain sections for beta-galactosidase activity, based on the co-expression of beta-galactosidase from Nph3-expressing cells. (uni-goettingen.de)

Gene targeting methods are established for several model organisms and may vary depending on the species used. (wikipedia.org)

Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter cell receives at least one copy of the plasmid. (addgene.org)

Our study aimed to characterize the differences in gut microbiota compositions and their roles in iron absorption between wild-type (WT) and CD163/pAPN double-gene-knockout (DKO) weaned piglets. (bvsalud.org)
In this article, we will describe how to perform specific gene disruption with TALENs in Xenopus tropicalis embryos, and eventually establish knockout Xenopus tropicalis line. (biomedcentral.com)

The ability to track microbes in real time in vivo is of enormous value for preclinical investigations in infectious disease or gene therapy research. (escholarship.org)
This study demonstrates for the first time the potential to simultaneously image multiple BLI reporter genes three dimensionally in vivo using approaches that provide unique information on spatial locations. (escholarship.org)
Yu J, Ruan Q, Nie X. Synthesis and characterization of atherosclerotic target anti-CD47 functionalized by nano- polyelectrolyte complexes between chitosan and hyaluronic acid and in vivo and in vitro targeting experiments. (edu.pl)

Such validation data is available from SigmaAldrich, one of the commercial distributors of the TRC library ( https://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/individual-genes.html ). (berterolab.com)
This design facilitates directional cloning of the shRNA into the targeting vector. (berterolab.com)
Our Lentivirus IPTG-Inducible shRNA Knockdown vectors are derived from the third-generation lentiviral vector system. (vectorbuilder.kr)
Our lentivirus IPTG-inducible shRNA knockdown vector has been validated for highly efficient target gene knockdown in the presence of IPTG as shown in Figure 1 below. (vectorbuilder.kr)
EGFP knockdown with the lentivirus IPTG-inducible shRNA vector system. (vectorbuilder.kr)

Gene targeting has been successfully applied to cattle, sheep, swine and many fungi. (wikipedia.org)
The targeting vector was cloned and its function was tested successfully in COS7 cells by Flag immunocytochemistry and beta-galactosidase staining. (uni-goettingen.de)

Given the lack of effective treatments, we constructed a bifidobacterial recombinant thymidine kinase (BF-rTK) -ganciclovir (GCV) targeting system (BKV) to meet this requirement and to explore antitumor mechanisms. (biomedcentral.com)

Often a reporter gene and/or a selectable marker is also required, to help identify and select for cells (or "events") where GT has actually occurred. (wikipedia.org)
Zhong J, Karberg M, Lambowitz AM. (2003 Mar) Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker . (uconn.edu)
Arakawa H, Lodygin D, Buerstedde JM (2001) Mutant loxP vectors for selectable marker recycle and conditional knock-outs . (uconn.edu)

Application of replication-deficient vectors leads to short-term expression, which makes these vectors highly attractive for cancer gene therapy. (genetherapynet.com)

The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)

Dekker M, Brouwers C, Te Riele H. (2003 Mar) Targeted gene modification in mismatch-repair-deficient embryonic stem cells by single-stranded DNA oligonucleotides . (uconn.edu)

Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)

Conclusion: The initial data from our investigation indicates the presence of Leptospira sp in rodent vectors, Rattus, Lophuromys and Praomys, which are the potential small mammalian reservoirs of this pathogen in Cote d'Ivoire. (bvsalud.org)

Predicting HIV Protease-Cleavable Peptides by Discrete Support Vector Machines. (stanford.edu)

Here we summarize the protocol that is used in our studies for gene disruption. (biomedcentral.com)

Alphaviruses are apt to be engineered to create replicon vectors which efficiently induce humoral and T-cell immune responses. (genetherapynet.com)
Therefore, the immune system does not 'see' it, enabling C3DNA to act like an endogenous gene in the nucleus, with enhanced persistence of expression, explained the biotech. (biopharma-reporter.com)

However gene targeting is also capable of inserting entire genes (such as transgenes) at the target site if the transgene is incorporated into the homology repair template that is used during gene-targeting. (wikipedia.org)

Mainly, two genes linA and linB encode dehydrochlorinase and dehalogenase enzymes involved in degradation of HCH. (ispub.com)

Although many bacterial microbiome analyses have been attempted using feces of wild animals, only a few studies have used NGS to screen for parasites. (biomedcentral.com)

Strategy for in situ detection of natural transformation-based horizontal gene transfer events. (readabstracts.com)

To create a gene-targeted organism, DNA must be introduced into its cells. (wikipedia.org)
There are limitations to the use of alphaviruses in the field of gene therapy due to their lack of targeting, however, through the introduction of variable antibody domains in a non-conserved loop in the structure of E2, specific populations of cells have been targeted. (genetherapynet.com)
However, some reports have claimed recently that aberrant gene expression followed by proteome alterations and neoantigen formation can result in iPSCs recognition by autologous T-cells. (biomedcentral.com)
MiR-137 inhibited cell proliferation and migration of vascular smooth muscle cells via targeting IGFBP-5 and modulating the mTOR/STAT3 signaling. (genscript.com)
cDNAs that resulted in poor expression in initial attempts will be moved into different expression vectors, or into eucaryotic expression systems such as COS and MEL (murine erythroleukemia) cells. (secsg.org)
Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
The insects will then be challenged with Serratia marcescens by microinjection of bacterial suspensions (~107 cells/ml) using a FemtoJet Microinjector (Eppendorf, Inc., Fremont. (citrusrdf.org)
The targeting vector was inserted into ES cells in culture and a Nph3-KI mouse line was generated (blastocyst injections and transplantations were performed in collaboration). (uni-goettingen.de)

Remaining cDNAs will be cloned into a custom TOPO (Invitrogen) vector. (secsg.org)

The nanovaccines (50-250 nm in size) are most efficient in terms of tissue targeting, staying in the bloodstream for a long time. (researchgate.net)

To also target low expressed essential genes, we included some variant steps that were expected to overcome the non-stringent regulation of the promoter carried by the expression vector used for the shotgun antisense libraries. (biomedcentral.com)

Investigating the biological role of PKCepsilon (e.g. required for macrophage activation and defence against bacterial infection, roles in cell division). (ximbio.com)

tomato flower abnormalities induced by stolbur phytoplasma infection are associated with changes of expression of floral development genes. (liverpool.ac.uk)
PKCe-/- animals appear normal and are generally healthy, though the female mice frequently develop a bacterial infection of the uterus. (ximbio.com)
This study demonstrated that bacterial abundance changed based on the season and specific parasitic infection status of the trapped mice. (biomedcentral.com)

The expression cassettes of the gRNAs are tandemly ligated into a single vector using the Golden Gate cloning method. (nature.com)

In the past few years, the preparation of polyelectrolyte complexes (PECs) through the electrostatic interaction of oppositely charged polyions, especially as a drug carrier or gene delivery system, has been widely studied by researchers. (edu.pl)
This dual-vector system utilizes a kill or rescue scheme to bypass the need for antibiotic or high-throughput screening. (sigmaaldrich.com)

Cancer Gene Therapy 27, pp. 785-798. (cardiff.ac.uk)

These include providing new ways to explore how genes control the formation and activity of different organisms. (elifesciences.org)

Anatomy15

Organisms55

Diseases6

Chemicals and Drugs53

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes63

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science8

Health Care12

Bacteria15

Pathogens8

Proteins11

RRNA10

Genomic7

Primers4

Sequences8

Homologous2

Mouse embryonic stem2

CRISPR11

Plasmids6

Lentiviral vectors3

Viral10

Genome13

Sequence10

Tumor9

Microbial1

Target genes4

GRNA4

Fragments1

Genetic6

Ribosomal1

Protein9

Species1

Plasmid1

Knockout2

Vivo3

ShRNA5

Successfully2

Recombinant1

Selectable marker3

Replication1

Transfection1

Oligonucleotides1

Approaches1

Rattus1

Peptides1

Disruption1

Immune2

Homology repair1

Encode1

Parasites1

Horizontal gene tra1

Cells8

Invitrogen1

Efficient1

Antisense1

Biological1

Infection3

Expression cassettes1

System2

Cancer gene1

Organisms1