Bacterial cyclic gmp. Medical search. Frequent questions

Several dinucleotide cyclases, including cyclic GMP-AMP synthase, and their involvement in STING-mediated immunity have been extensively studied. (unav.edu)
Structural Basis for the Catalytic Mechanism of DncV, Bacterial Homolog of Cyclic GMP-AMP Synthase. (nurekilab.net)
Recent studies have characterized ancient forms of cyclic GMP-AMP (cGAMP) synthase (cGAS)-like receptors (cGLRs) in bacterial and Drosophila immunity. (ufl.edu)
In the mammalian innate immune system, the protein cyclic GMP-AMP synthase (cGAS) detects foreign or damaged "self" DNAs. (sciencedaily.com)
We re-established the decreased survival in the RAW 264.7 macrophage cell line and determined that the cyclic-di-GMP-binding cellulose synthase BcsA was responsible for decreased survival in macrophages. (etsu.edu)
Cyclic GMP-AMP synthase (cGAS) senses foreign DNA to produce 2'3′-cyclic GMP-AMP (2'3′-cGAMP). (knoblauchpublishing.com)
We treated RAW 264.7 macrophages with conditioned media (CM) generated from planktonic or biofilm cultured SA in combination with agonists or inhibitors of the cyclic GMP-AMP synthase (cGAS)/STING pathway. (qxmd.com)
In vivo Mtb aerogenic infection induced the upregulation of the STING and cGAS (cyclic GMP-AMP synthase) genes, and Ifn-β pulmonary expression was dependent on both sensors. (elsevierpure.com)

Cyclic di-GMP-I riboswitches are a class of riboswitch that specifically bind cyclic di-GMP, which is a second messenger that is used in a variety of microbial processes including virulence, motility and biofilm formation. (wikipedia.org)
Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins in Vibrio alginolyticus unveils key diguanylate cyclases controlling multiple biofilm-associated phenotypes. (bvsalud.org)
The biofilm lifestyle is critical for bacterial survival and proliferation in the fluctuating marine environment . (bvsalud.org)
Cyclic diguanylate (c-di-GMP) is a key second messenger during bacterial adaptation to various environmental signals, which has been identified as a master regulator of biofilm formation. (bvsalud.org)
However, little is known about whether and how c-di-GMP signaling regulates biofilm formation in Vibrio alginolyticus , a globally dominant marine pathogen. (bvsalud.org)
Guided by protein homology, conserved domains and gene context information, a representative subset of 22 c-di-GMP metabolic proteins were selected to determine which ones affect biofilm -associated phenotypes . (bvsalud.org)
The results indicated that overexpression of four c-di-GMP metabolic proteins , including VA0356, VA1591 (CdgM), VA4033 (DgcB) and VA0088, strongly enhanced rugose colony morphotypes and strengthened Congo Red (CR) binding capacity, both of which are indicators of biofilm matrix overproduction. (bvsalud.org)
This work provides a robust foundation for the study of the molecular mechanisms of c-di-GMP in the biofilm formation of V. alginolyticus. (bvsalud.org)
c-di-GMP is a signaling molecule found in almost all bacteria that acts to regulate an extensive range of processes including antibiotic resistance, biofilm formation and virulence. (dundee.ac.uk)
The understanding of how c-di-GMP signaling controls aspects of antibiotic resistant biofilm development has suggested approaches whereby alteration of the cellular concentrations of the nucleotide or disruption of these signaling pathways may lead to reduced biofilm formation or increased susceptibility of the biofilms to antibiotics. (dundee.ac.uk)
Quorum sensing, C-di-GMP, and sometimes s-RNA regulate the biofilm formation. (learnlifescience.com)
Subramani (2019) said that Quorum sensing (QS) is a process of intercellular signaling or cell-cell communication and a vital regulatory mechanism for coordinating biofilm formation including common activities and physiological processes such as symbiosis, formation of spores or fruiting bodies, antibiotics synthesis, genetic competence, apoptosis, and virulence in many bacterial species using extracellular QS signaling molecules, which is often referred to as autoinducers. (learnlifescience.com)
It is a second messenger that modulates a variety of bacterial growth phenotypes including biofilm formation. (learnlifescience.com)
We also found that the wild-type P. aeruginosa PAO1 cells attached on soft (40:1) PDMS have higher level of intracellular cyclic dimeric guanosine monophosphate (c-di-GMP), a key regulator of biofilm formation, compared to those on stiff (5:1) PDMS surfaces. (syr.edu)
Collectively, the results from this study suggest that P. aeruginosa can sense the stiffness of substrate material during attachment and respond to such mechanical cues by adjusting c-di-GMP level and thus the following biofilm formation. (syr.edu)
The widespread bacterial second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) is an important regulator of biofilm formation, virulence and cell differentiation. (archive.org)
The ETT provides a conduit for bacterial access to the lower respiratory tract and a substratum for biofilm formation, both of which lead to VAP. (springeropen.com)
An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. (inrs.ca)
Nitrate represses production of an important biofilm component, curli, and activates flagella via the modulation of intracellular cyclic-di-GMP levels. (knoblauchpublishing.com)
Finally, we propose that PggH could modulate the activities of PDE-As and the intracellular concentration of c-di-GMP, resulting in phenotypic changes including in biofilm formation. (knoblauchpublishing.com)
Bacterial two-hybrid results showed that the Par-like proteins interact with each other and the chemotaxis system, and that ParP interacts with DipA, a phosphodiesterase which degrades cyclic-di-GMP and is important for biofilm dispersion and chemotaxis. (uwm.edu)
Biofilm formation on the implant impairs the immune response, leading to bacterial persistence. (qxmd.com)
Description: Cyclic-di-GMP is a STING agonist and a ubiquitous second messenger that regulates biofilm formation, motility, and virulence in diverse bacterial species. (oegterm.at)

Upon DNA detection, cGAS synthesizes cyclic GMP-AMP (cGAMP), the second messenger molecule that activates the cGAS-STING signaling pathway to fight infections, inflammatory diseases, and cancers. (sciencedaily.com)
However, mice deficient for STING or cGAS presented a similar outcome to wild-type controls, with no major alterations in body weight gain, bacterial burden, or survival. (elsevierpure.com)

A large number of genes coding for enzymes predicted to synthesize and degrade 3′-5′-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. (inrs.ca)

A group of enzymes that are dependent on cyclic GMP and catalyzes the phosphorylation of serine or threonine residues of proteins. (harvard.edu)
Insertion of proteins into the bacterial outer membrane is mediated by a dedicated chaperone machinery. (unibas.ch)
Here, a large set of 63 proteins were predicted to participate in c-di-GMP metabolism ( biosynthesis or degradation) in a pathogenic V. alginolyticus strain HN08155. (bvsalud.org)
By comparing phenotypic differences between the wild-type and mutants or overexpression strains , we found that 22 c-di-GMP metabolic proteins can separately regulate different phenotypic outputs in V. alginolyticus. (bvsalud.org)
Overall, the high abundance of c-di-GMP metabolic proteins in V. alginolyticus suggests that c-di-GMP signaling and regulatory system could play a key role in its response and adaptation to the ever-changing marine environment . (bvsalud.org)
Bacterial toxins can be single proteins or oligomeric protein complexes that are organized with distinct AB structure-function properties. (springer.com)
Co-immunoprecipitation, bacterial two-hybrid and pull-down analyses showed that PilH and PilG were able to interact with district subsets of proteins that potentially account for their regulatory impact. (biomedcentral.com)

In conclusion, we propose that PdeA contributes to bacterial morphology and virulence in B. abortus, but it is not crucial for polarity and asymmetric growth. (unamur.be)
Targeting bacterial virulence factors is a wise option, as it can mitigate the pathogenicity of bacteria without eradicating them. (qxmd.com)
Sortase A (SrtA), which is critical for bacterial adhesion, invasion, and immune evasion, is a promising target for virulence inhibition in S. aureu s. (qxmd.com)

We describe a simple high-throughput bioreporter protocol, based on green fluorescent protein (GFP), whose expression is under the control of the c-di-GMP responsive promoter cdrA, to rapidly screen for small molecules with the potential to modulate c-di-GMP cellular levels in Pseudomonas aeruginosa (P. aeruginosa). (dundee.ac.uk)
Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem Environmental Microbiology , vol. 16 , nº 5. (inrs.ca)
We found that increased levels of c-di-GMP in Pseudomonas aeruginosa improve fitness in the presence of imipenem, even when grown as planktonic cells. (inrs.ca)

A bacteriophage that infects C. difficile is predicted to carry a cyclic di-GMP-I riboswitch, which it might use to detect and exploit the physiological state of bacteria that it infects. (wikipedia.org)
The discovery of this riboswitch class answers the question of how genes are regulated in response to cyclic di-GMP levels in many different bacteria. (wikipedia.org)
However, some bacteria in which cyclic di-GMP has been studied lack cyclic di-GMP-I riboswitches, e.g. (wikipedia.org)
Here we show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link between c-di-GMP and glycogen metabolism in bacteria. (nih.gov)
Overall, we identify a function of c-di-GMP in controlling energy storage metabolism in bacteria, which is widespread in Actinobacteria. (nih.gov)
The bacterial origin of mitochondria suggests that inflammatory pathways found in cytosol-invading bacteria may be relevant to mitochondrial-driven inflammation after MOMP. (nature.com)
Some of these mitochondrial DAMPs share similarities with pathogen-associated molecular patterns (PAMPs) found in bacteria and may be derived from their bacterial ancestors. (nature.com)
Most c-di-GMP-dependent signaling pathways control the ability of bacteria to interact with abiotic surfaces or with other bacterial and eukaryotic cells (Romling et al, 2013). (learnlifescience.com)
We strive to analyze, describe, and predict how bacteria process information about metabolic status, through the use of metabolic signals and secondary messengers, into a re-programming of the bacterial phenotype. (dtu.dk)
As a proof-of-principle, we demonstrate that CensYBL is functional in detecting fluctuations in intracellular c-di-GMP levels in the Gram-negative model bacteria Escherichia coli and Salmonella enterica serovar Typhimurium. (archive.org)
However, despite the numerous studies on bacterial motility in other Gram-negative bacteria, only limited work has been carried out examining the motility regulation in Xcc . (biomedcentral.com)

In this study, we tested five bacterial diguanylate cyclases from the Gram-negative bacterium Salmonella Enteritidis, identifying AdrA as the most potent inducer of a STING-mediated IFN response. (unav.edu)
Using deletion mutants of c-di-GMP diguanylate cyclases and phosphodiesterases, we show that c-di-GMP dependent dimerization of CBldD-YPet results in fluorescence complementation reflecting intracellular c-di-GMP levels. (archive.org)
The concentration of cyclic-di-GMP is regulated by the presence of external stimuli, sensor CMEs (diguanylate cyclases, DGCs, and phosphodiesterases, PDEs), and cyclic-di-GMP binding effectors. (etsu.edu)
synthesized by GGDEF domain-containing diguanylate cyclases and degraded by HD-GYP domain-containing phosphodiesterases (PDEs) to 2 GMP or by EAL domain-containing PDE-As to 5′-phosphoguanylyl-(3′,5′)-guanosine (pGpG). (knoblauchpublishing.com)

On the horizon, novel insights into metabolism and energy homeostasis reveal guanosine 3′,5′-cyclic monophosphate (cGMP) signaling circuits as emerging targets for antiobesity pharmacotherapy. (eddoctor24h.com)

inactive c-di-GMP phosphodiesterase-like EAL domain protein [Ensembl]. (ntu.edu.sg)
A mutation in the cyclic-di-GMP phosphodiesterase catalytic site of PdeA does not generate the coccoid morphology and the ΔpdeA mutant kept the ability to recruit markers of new and old poles. (unamur.be)

Regulatory systems that utilize intracellular cyclic di-GMP (c-di-GMP) as a second messenger are one such class of target. (dundee.ac.uk)

Some of these mitochondrial-activated inflammatory pathways can be traced back to the bacterial ancestry of mitochondria. (nature.com)
For instance, mtDNA and bacterial DNA are highly similar thereby activating similar cell autonomous immune signalling pathways. (nature.com)
Immediate activation of chemosensory neuron gene expression by bacterial metabolites is selectively induced by distinct cyclic GMP-dependent pathways in Caenorhabditis elegans. (harvard.edu)
Bacterial resistance to traditional antibiotics has driven research attempts to identify new drug targets in recently discovered regulatory pathways. (dundee.ac.uk)
Further understanding of the related genes and pathways will provide new insights into bacterial mechanosensing and help develop better antifouling materials. (syr.edu)

Recently, signaling by GCC and its downstream effector, cyclic GMP (cGMP) has emerged as a principal regulator of proliferation in human colon cancer cells. (jefferson.edu)

Bacterial QS regulation is established through a wide range of signals such as oligopeptides, N-acyl homoserine lactones, furanose borate, hydroxy palmitic acid methyl ester, and methyl decanoic acid. (learnlifescience.com)
Cyclic-di-GMP plays crucial role in the cell cycle regulation of the α-Proteobacterium Caulobacter crescentus. (unamur.be)
Regulation of the bacterial second messenger cyclic-di-GMP in Salmonella Typhimurium allows it to delicately alter phenotypes to optimize invasion and survive intracellularly in epithelial cells and macrophages to become virulent and cause infection. (etsu.edu)
This study aimed to further investigate the regulation of cyclic-di-GMP for survival in macrophages and epithelial cells. (etsu.edu)
IMPORTANCE Recent studies provided important insight into our understanding of the role of c-di-GMP signaling and the regulation of enteric biofilms. (knoblauchpublishing.com)
Cyclic GMP plays a central role in the regulation of many physiologic responses, including the modulation of immune responses. (livonlabs.com)

Main efflux pumps or β-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. (inrs.ca)
We have developed a powerful computational framework for revealing the genetic basis of bacterial traits by correlating the inheritance patterns of genes and phenotypes across all the sequenced bacterial genomes (Jim et al. (columbia.edu)
Furthermore, we have developed a genome-wide epistasis analysis framework that efficiently reveals the organization of these genes within signaling and regulatory networks such as the Rcs phosphorelay pathway and the cyclic-di-GMP second-messenger system. (columbia.edu)

in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)

Cyclic di-GMP-I riboswitches are the first kind of riboswitch to be discovered whose role is not primarily in regulating metabolism, but is instead part of signaling. (wikipedia.org)
Some homologs of the c-di-GMP-I riboswitch structure actually function a riboswitches that recognize another signaling molecule, cyclic AMP-GMP. (wikipedia.org)
As part of nitric oxide-cyclic GMP transduction signaling operative in higher eukaryotes and involved in the long-term potentiation, a soluble guanylyl cyclase has also been detected in this lower eukaryote. (ias.ac.in)
Despite an improved understanding of how c-di-GMP signaling regulates S. Typhimurium biofilms, the processes that affect the intracellular c-di-GMP levels and the formation of multicellular communities in vivo during infections remain unknown. (knoblauchpublishing.com)

Bacterial toxins are often characterized based upon the secretion mechanism that delivers the toxin out of the bacterium, termed types I-VII. (springer.com)
Quorum sensing is a type of bacterial communication that occurs via the secretion of autoinducer molecules (5). (kenyon.edu)

Giannella RA (1981) Pathogenesis of acute bacterial diarrheal disorders. (springer.com)

Field M, Graf LH Jr, Laird WJ, Smith PL (1978) Heat-stable enterotoxin of Escherichia coli: In vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. (springer.com)
Once inside, it activated adenyl cyclase, causing the concentration of cyclic-AMP to increase. (kenyon.edu)

Previous studies established that maintenance of low cyclic-di-GMP concentrations is required for survival in macrophages and that the deletion of 3 active PDEs reduces this survival. (etsu.edu)
Finally, we identified an active DGC whose deletion within the 3xKO PDEs restores survival levels, suggesting that this enzyme is responsible for the synthesis of cyclic-di-GMP during macrophage infection. (etsu.edu)

A second class of riboswitch that binds cyclic di-GMP is called the cyclic di-GMP-II riboswitch. (wikipedia.org)

We ascribe these effects to ACSP50_2473 , which is localized downstream of amlE and presumably involved in the metabolism of the second messenger c-di-GMP. (frontiersin.org)
SE50/110, but that its metabolism is coupled to the nucleotide messenger system of c-di-GMP. (frontiersin.org)

C-di-GMP-specific biosensors that allow detection and visualization of c-di-GMP levels in living cells are key to our understanding of how c-di-GMP fluctuations drive cellular responses. (archive.org)

Since excess pGpG feedback inhibits PDE-A activity and thereby can lead to the uncontrolled accumulation of c-di-GMP, a PDE that degrades pGpG to 2 GMP (PDE-B) has been presumed to exist. (knoblauchpublishing.com)

Together, our findings show that c-di-GMP levels modulate fitness of planktonic cultures in the presence of imipenem. (inrs.ca)

Bacterial toxins damage the host at the site of bacterial infection or distant from the site. (springer.com)
Curli, a major component of the bacterial biofilms in the intestinal tract, activates pattern recognition receptors and triggers joint inflammation after infection with Salmonella enterica serovar Typhimurium. (knoblauchpublishing.com)
Positive results do not rule out bacterial infection or co- infection with other viruses. (oegterm.at)
Previous diagnostic criteria for acute bacterial sinusitis in children were acute upper respiratory tract infection (URI) with either nasal discharge and/or daytime cough for longer than 10 days or severe onset of fever, purulent nasal discharge, and other respiratory symptoms for 3 or more consecutive days. (medscape.com)

Vitamin C makes bacterial membranes more permeable to some antibiotics. (livonlabs.com)

P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. (inrs.ca)

Cells with low c-di-GMP levels are consequently more sensitive to this antibiotic. (inrs.ca)
Antibiotic susceptibility is one of the most important yet poorly characterized bacterial phenotypes. (columbia.edu)

Imaging tests are not recommended for children with uncomplicated acute bacterial sinusitis, although children with suspected orbital or CNS complications should undergo CT scanning of the paranasal sinuses. (medscape.com)

Surprisingly, deletion of all predicted cyclic-di-GMP synthesizing or degrading enzymes did not drastically impair the growth of B. abortus, nor its ability to grow inside cell lines. (unamur.be)

We describe structures of apo and c-di-GMP-bound GlgX and, strikingly, their comparison shows c-di-GMP induces long-range conformational changes, reorganizing the catalytic pocket to an active state. (nih.gov)

Detecting and responding to foreign DNA from bacterial and viral pathogens is one of the most fundamental mechanisms for host defense," said Zhang, co-senior author. (sciencedaily.com)
Treatment of acute sinusitis consists of providing adequate drainage of the involved sinus and appropriate systemic treatment of the likely bacterial pathogens. (medscape.com)

Sigma-54 interaction domain, Bacterial regulatory protein, Propionate catabolism activator [Interproscan]. (ntu.edu.sg)
LysR substrate binding domain, Bacterial regulatory helix-turn-helix protein [Interproscan]. (ntu.edu.sg)

Further studies on how cyclic-di-GMP regulates Salmonella Typhimurium intracellular survival could lead to identifying a potential alternative drug target for treating its infections. (etsu.edu)

In contrast, pretreatment with Ca 2+ ionophores, which elevated Ca 2+ levels in L. donovani, significantly enhanced the cyclic GMP level as well as the infectivity of the parasites. (ias.ac.in)
Vitamin C enhances cyclic GMP levels in lymphocytes. (livonlabs.com)

Cyclic GMP-Dependent Protein Kinases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Cyclic GMP-Dependent Protein Kinases" by people in Harvard Catalyst Profiles by year, and whether "Cyclic GMP-Dependent Protein Kinases" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Cyclic GMP-Dependent Protein Kinases" by people in Profiles. (harvard.edu)
Bacterial toxins also catalyze the non-covalent modification of host protein function or can modify host cell properties through direct protein-protein interactions. (springer.com)

Consistently, the mutants of fleQ and wspF, which have similar high-level c-di-GMP as the oprF mutant, exhibited defects in response to PDMS stiffness during attachment. (syr.edu)

c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. (nih.gov)
Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. (learnlifescience.com)

Cyclic GMP is important for normal cell proliferation and differentiation. (livonlabs.com)

In this review, we discuss how mitochondria can initiate inflammation during cell death highlighting parallels with bacterial activation of inflammation. (nature.com)

Anatomy11

Organisms11

Chemicals and Drugs81

Phenomena and Processes12

Synthase8

Biofilm23

CGAS2

Diguanylic acid1

Proteins7

Virulence3

Pseudomonas3

Bacteria11

Diguanylate cyclases4

Monophosphate1

Phosphodiesterase2

Intracellular cyclic1

Pathways5

CGMP1

Regulation6

Genes3

Regulator1

Signaling4

Secretion2

Pathogenesis1

Concentration2

PDEs2

Binds1

Metabolism2

Detection1

Degrades1

Modulate1

Infection4

Antibiotics1

Aeruginosa1

Antibiotic2

Acute bacterial1

Enzymes1

Catalytic1

Pathogens2

Regulatory2

Infections1

Levels2

Protein4

Mutants1

Regulators2

Proliferation1

Activation1