Loading...
  • recombination
  • However, it requires means to overcome the cellular mechanisms that normally restrict recombination to dif sites harbored by a chromosome dimer and, in the case of integrative mobile elements, to convert dedicated tyrosine resolvases into integrases. (asmscience.org)
  • swarmer
  • The swarmer cell interaction matrix contains two prominent diagonals ( Fig. 1A ). (sciencemag.org)
  • On cell division, one pole yields a swarmer cell, and the other pole yields a nonmotile stalked cell (Fig. 1 B ), each with different cell fates ( 1 ). (pnas.org)
  • Thus, the swarmer-to-stalked cell transition is coincident with the initiation of DNA replication. (pnas.org)
  • During S phase, the stalked cell elongates and differentiates a new swarmer pole by activating a flagellar transcriptional cascade ( 5 ). (pnas.org)
  • Phosphorylated CtrA (CtrA≈P) is present in the swarmer cell, rapidly disappears at the swarmer-to-stalked cell transition, and accumulates again in the predivisional cell. (pnas.org)
  • Each cell division in this organism is asymmetric, producing a daughter stalked cell in S phase and a daughter swarmer cell in G1. (pnas.org)
  • After cell division, CtrA~P continues to repress DNA replication in daughter swarmer cells but is rapidly dephosphorylated and degraded in daughter stalked cells, permitting the immediate initiation of DNA replication. (pnas.org)
  • consists
  • Analysis of Hi-C data and polymer modeling indicates that the Caulobacter crescentus chromosome consists of multiple, largely independent spatial domains that are probably composed of supercoiled plectonemes arrayed into a bottle brush-like fiber. (sciencemag.org)
  • Genome Instability
  • Because endogenous (metabolically-caused) DNA damage is very frequent, occurring on average more than 60,000 times a day in the genomes of human cells, any reduced DNA repair is likely an important source of genome instability. (wikipedia.org)
  • DnaA
  • They bind to DnaA-ADP and DnaA-ATP with equal affinities and are bound by DnaA throughout most of the cell cycle and forms a scaffold on which rest of the orisome assembles. (wikipedia.org)
  • helicase
  • After DnaB translocates to the apex of each replication fork, the helicase both unwinds the parental DNA and interacts momentarily with primase . (wikipedia.org)
  • processes
  • Chromosomal DNA must be highly compacted to fit within the tiny volume of the cell, while at the same time it must maintain a conformation that allows critical cellular processes access to the genome. (sciencemag.org)
  • methylation
  • Because the methylation reactions occur after replication, adenine residues within GATC sequences in the newly synthesized strand remain unmethylated for a period of time. (pnas.org)
  • mechanism
  • It is clear that a mechanism exists to prevent premature reinitiation until the proper time in the cell cycle is reached ( 2 ). (pnas.org)
  • Our data challenge this model, indicating instead that the cell uses a sophisticated symmetry-breaking mechanism such that CtrA is differentially phosphorylated across the predivisional cell. (pnas.org)
  • genetic
  • Using a combination of mathematical modeling, single-cell microscopy, and genetic manipulation, we demonstrate that this gradient is produced by the polarly localized phosphorylation and dephosphorylation of CtrA. (pnas.org)
  • When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase. (wikipedia.org)
  • citation needed] Wilhelm Roux suggested that each chromosome carries a different genetic load. (wikipedia.org)
  • Defects
  • Defects in blood cell development are also prominent, and it is not known how these may affect the brain. (wikipedia.org)
  • predivisional cell
  • We report here that ctrA expression is under the control of two promoters: a promoter (P1) that is active only in the early predivisional cell and a stronger promoter (P2) that is active in the late predivisional cell. (pnas.org)
  • We propose that the P1 promoter is activated after the initiation of DNA replication in the early predivisional cell. (pnas.org)
  • molecules
  • The entire assembly of molecules involved in DNA replication on each arm is called a " replisome . (wikipedia.org)
  • A recently developed, high-throughput (HT) method for identifying ligands involved in chemotaxis can also be used for identifying other types of bacterial signal molecules that bind sensor domains, according to Monica Gerth of the University of Otago in Dunedin, New Zealand, and her collaborators. (asmscience.org)
  • single
  • Along with single-cell genomic sequencing, the technologies collectively known as "meta'omics," including metagenomics, metatranscriptomics, metabolomics, and metaproteomics, are powerful new tools for learning how microbes in, on, and around us affect our health and well-being. (asmscience.org)