Bacterial cell protein clone cells. Medical search. Frequent questions

Based on an analysis of the relative quantity of each transcript and an assay for chitin-degrading activity of recombinant proteins, a chitin degradation system driven by 19 chitinolytic enzymes was proposed. (bvsalud.org)
Thus, there is a big demand for high-copy-number plasmids for useful gene manipulation and overproduction of recombinant proteins in Leuconostoc . (biomedcentral.com)
The effectiveness of the isolated high-copy-number plasmid for the overproduction of recombinant proteins was successfully demonstrated with two protein models Glutathione-S-transferase (GST) and α-amylase. (biomedcentral.com)
In particular, expression plasmids are one of the most fundamental systems for genetic manipulations and protein production, especially to carry foreign DNA and to produce recombinant proteins in a host. (biomedcentral.com)

These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
E. coli strain GB08-red enables recombineering of plasmids and BACs without the necessity to maintain an expression plasmid in the cells since the necessary. (biosave.com)
The following is the method used by the USU team to insert the plasmids containing the PHB biobricks into the cells. (igem.org)
TABLE 1 Bacterial strains and plasmids found in this?research mutantE. (careersfromscience.org)

Many of these clones are characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
Continued new insights into host-parasite interactions at the molecular level will be achieved by combining clonal analysis of bacterial pathogens with large-scale comparative sequencing of virulence genes. (cdc.gov)
Moreover, the distinct clones are frequently characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
Mutations in the three SHANK genes encoding for postsynaptic scaffolding proteins have been shown to represent risk factors for autism spectrum disorders and other neurodevelopmental disorders. (nature.com)
As several synaptic proteins like neurexins and neuroligins are interaction partners of the SHANKs and associate with both disorders, the question arises if variants in the SHANK genes themselves can be associated with SCZ. (nature.com)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
The ability to engineer silk genes and recombinantly produce spider silk proteins is key to generation of tailored silk-based biomaterials. (uwyo.edu)
Spider silk-like genes are easily engineered for silk protein recombinant production due to the native modular repetitive nature of spider silk proteins. (uwyo.edu)
The term "competent" is to describe a cell state in which there exist gaps or openings in the cell wall which will allow the plasmid containing the target genes to enter into the cell. (igem.org)
Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
When the genetic material within the living cells, i.e. genes are working properly, the human body can develop and function smoothly. (faqs.org)
Even though all cells of a multicellular organism have the same set of genes, cells in various tissues produce different sets of proteins. (americascupmagazine.com)
A gene encoding an endopeptidase from FW213 continues to be cloned and proven to possess high series homology to genes encoding mammalian metalloendopeptidases. (careersfromscience.org)
Bacteria sense these microenvironments and adapt by up or down regulating the expression of certain genes to influence different cell processes. (the-scientist.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

gene cloning ( DNA cloning ) The production of exact copies ( clones ) of a particular gene or DNA sequence using genetic engineering techniques. (encyclopedia.com)
thus, a bacterial host will give rise to a colony of cells containing the cloned target gene. (encyclopedia.com)
human insulin, for example, is now produced by bacteria containing the cloned insulin gene. (encyclopedia.com)
A foreign protein is first expressed in fusion with one of the phage capsid proteins by cloning the foreign DNA sequence in frame with the gene encoding the chosen capsid protein [ 22 ]. (biomedcentral.com)
Combining these new datasets with existing published gene expression and protein expression data have identified selected panels of novel biomarkers which have been evaluated on over 100 cervical cancer and pre-cancer cases using standard immunohistochemistry approaches. (europa.eu)
High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression. (the-scientist.com)
The pTriEx-1 vector allows high-level gene expression in mammalian, E. coli , and insect cells. (the-scientist.com)
The acquired sequence is not predicted to express any protein even though it contains one exon of the VAMP1 gene. (usda.gov)
Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. (gomolecular.pk)
The bacterial silk clone cells were harvested following expression of the recombinant silk gene. (uwyo.edu)
The technique required three elements: the gene to be transferred, a host cell in which the gene is to be inserted, and a vector for transferring the gene to the body. (faqs.org)
Suppose, for example, that one wishes to insert the insulin gene into a bacterial cell. (faqs.org)
Scientists compare gene expression patterns between infected and uninfected host cells, gaining important information about the cellular responses to infectious disease. (americascupmagazine.com)
This gene, specified and the proteins it encodes had been characterized in a variety of dental streptococci strains, including stress found in this research. (careersfromscience.org)

Early work on the clonal nature of bacterial pathogens was conducted largely with Escherichia coli , through a framework supplied by serotyping of one or a few polymorphic surface antigens ( 7 , 8 ). (cdc.gov)
In this study, two roughly 60 kDa chimeric flagelliform-dragline Spider Silk-Like Proteins (SSLPs), differing slightly in their amino acid sequences, were produced in recombinant Escherichia coli clones. (uwyo.edu)
The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48. (ox.ac.uk)
In some cases, the mast cell TNF-alpha response was triggered when these cells directly bound FimH on the surface of Escherichia coli. (ox.ac.uk)
Compared to other well-used cell factories inculding Escherichia coli , Bacillus subtilis as well as Pichia pastoris and Saccharomyces cerevisiae , etc. (biomedcentral.com)

Purified CyHV-2 ORF55 protein was obtained by prokaryotic expression, and the interacting peptide was screened out using phage display. (biomedcentral.com)
Reverse transcription quantitative PCR results demonstrated high expression of an actin-binding Rho-activating protein in the latter stages of virus-infected cells, and molecular docking, cell transfection and coimmunoprecipitation experiments confirmed that it interacted with the ORF55 protein. (biomedcentral.com)
Here we used serological expression cloning to identify commensal bacterial proteins that could contribute to the pathogenesis of IBD. (nih.gov)
The pFN2A and pFN2K (GST) Flexi® Vectors are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. (gomolecular.pk)
The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. (gomolecular.pk)
Reusable gluthathione (GST-affinity) resins can be used with the pFN2 (GST) Flexi® Vectors for standard protein purification and prokaryotic expression applications. (gomolecular.pk)
The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. (gomolecular.pk)
[ 10 ] Multiple biofilm-specific mechanisms are operated simultaneously in a reversible and transient manner contributing to the high levels of antibiotic resistance of biofilms, and these are distinct from the well-characterized intrinsic resistance mechanisms (e.g., expression of antibiotic-degrading enzymes, inducible decrease in antibiotic influx, inducible increase in antibiotic efflux and alteration in antibiotic target sites) employed by planktonic cells. (medscape.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
In vivo expression of alternatively spliced forms of integrin-associated protein (CD47). (bdbiosciences.com)
Contactin/F3, a cell adhesion molecule, has been shown to interact with and enhance surface expression of sodium channels Na v 1.2 and Na v 1.9. (jneurosci.org)
The expression of contactin and Na v 1.3 in the HEK-Na v 1.3 stable cell line significantly increases the current density. (jneurosci.org)
Proteomics may be used to study which proteins are expressed under various conditions within a single cell type or to compare protein expression patterns between different organisms. (americascupmagazine.com)
A recent and developing proteomic analysis relies on identifying proteins called biomarkers, whose expression is affected by the disease process. (americascupmagazine.com)
Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65-kDa heat-shock protein: selective in vivo expansion of T cells bearing defined receptors. (ox.ac.uk)
We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. (ox.ac.uk)
Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. (ox.ac.uk)
BL21(pLysS) cells were utilized as expression-competent hosts. (careersfromscience.org)
Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)

Protein and peptide interaction prediction and dot-blot overlay assay confirmed that peptides identified by phage display could interact with the ORF55 protein. (biomedcentral.com)
Comparing the peptides to the National Center for Biotechnology Information database revealed four potential interacting proteins. (biomedcentral.com)
the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. (gomolecular.pk)
Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)

Does NEBExpress® E. coli Lysis Reagent lyse gram-negative bacterial cells? (neb.com)
This is a false colored mesoscopic image of two strains of E. coli expressing different fluorescent proteins. (the-scientist.com)

By numerous specific protein-protein interactions, SHANKs are either directly or indirectly linked to other structural proteins, cell adhesion molecules, receptors, ion channels and to actin-interacting proteins at the postsynaptic density. (nature.com)
Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)
Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)
The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)
Because of their high numbers, red blood cells express an average of 90% of the CR1 receptors despite their having a lower number of antigenic sites per cell. (medscape.com)

Jiang P, Lagenaur CF, Narayanan V. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. (bdbiosciences.com)
De novo formation of an adherens junction (AJ) between single epithelial cells requires coordinated, spatial actin dynamics, but the mechanisms steering nascent actin polymerization for cell-cell adhesion initiation are not well understood. (rupress.org)
Here we investigated real-time actin assembly during daughter cell-cell adhesion formation in human breast epithelial cells in 3D environments. (rupress.org)

Microscopic analysis of cell and colony structure indicates that CmrRST promotes the formation of elongated bacteria arranged in bundled chains, which may contribute to bacterial migration on surfaces. (nih.gov)
Hence, bacterial population genetic research was originated by population geneticists interested in bacteria, rather than bacterial geneticists or medical microbiologists interested in population genetics ( 1 , 2 ). (cdc.gov)
They observed that when viruses entered the bacteria, the viral DNA was cut into small pieces and inactivated by certain bacterial enzyme. (faqs.org)
Most of all, L. citreum is also GRAS status and a food-grade bacteria, so it can be a very attractive host for the delivery of pharmaceutical proteins into human or animals with a higher impact in Pharma and Biotech induestries. (biomedcentral.com)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
This extracellular matrix glues bacteria to surfaces and serves as a slimy shield, protecting cells in the interior from predators and antibiotics. (the-scientist.com)

Our findings indicate that C. difficile employs phase variation of the CmrRST signal transduction system to generate phenotypic heterogeneity during infection, with concomitant effects on bacterial physiology and pathogenesis. (nih.gov)

In the process of cloning the entire Md11 genome in a bacterial artificial chromosome (BAC) vector, we obtained an infectious clone in which the entire terminal repeat short segment (TRS) was replaced by a portion of the duck genome. (usda.gov)

During viral infection, the ORF55 protein exerts its biological function through interactions with host proteins. (biomedcentral.com)
A common theme demonstrated by these analyses is that distinct bacterial clones are responsible for disease outbreaks and increases in infection frequency. (cdc.gov)
One of the primary themes emerging from this research is that distinct bacterial clones have been responsible for several infection outbreaks ( Table ). (cdc.gov)
This finding provides direct evidence that host genome acquisition by MDV actually occurs during virus replication, and that one or more such MDV genomes with host sequences may exist within MDV viral stocks, which tend to be polyclonal due to the strictly cell-associated nature of its infection process. (usda.gov)
[ 16 ] After antibiotic treatment, only persister cells may survive, creating the reservoirs of surviving cells that may regrow to cause a relapsing chronic infection, which has been clearly described for cystic fibrosis-associated lung infections caused by P. aeruginosa [ 17 ] and for candidiasis by C. albicans . (medscape.com)
Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice. (bdbiosciences.com)
Several recent studies have reported that mast cells play a critical role in innate immunity in mice by releasing tumor necrosis factor alpha (TNF-alpha) to recruit neutrophils to sites of enterobacterial infection. (ox.ac.uk)
Antibodies are proteins that help the body fight infection. (msdmanuals.com)

A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. (jax.org)
The WASP quality has 12 exons and encodes a 502 amino-acid protein (WASP), which is overwhelmingly communicated in nonerythroid hematopoietic cells [ 3 ]. (alliedacademies.org)

Transfection of HEK-Na v 1.3 cells with contactin increases the amplitude of the current threefold without changing the biophysical properties of the channel. (jneurosci.org)

Because serotype analysis of relatively few surface structures does not provide robust data for estimating overall levels of chromosomal diversity and relationships among strains, the primary research tool used to examine the population genetics of emerging bacterial pathogens has been multilocus enzyme electrophoresis ( 10 , 11 ). (cdc.gov)
Multiple strains of colitic mice had elevated serum anti-flagellin IgG2a responses and Th1 T cell responses to flagellin. (nih.gov)

Population genetic theory and tools and related strategies have been used to investigate bacterial pathogens that have contributed to recent episodes of temporal variation in disease frequency and severity. (cdc.gov)
Because substantial interclonal variance exists in relative virulence, molecular population genetic studies have led to the concept that the unit of bacterial pathogenicity is the clone or cell line. (cdc.gov)
Although comprehensive, unambiguous understanding of the host and parasite factors mediating these processes is not available for any infectious agent, population genetic research in the last 10 years has provided noteworthy new information about the bacterial side of the equation. (cdc.gov)
Population genetic study of bacterial pathogens arose largely as an offshoot of research designed to address questions of longstanding interest to students of the molecular evolutionary processes in higher eukaryotic organisms. (cdc.gov)
Recent genetic data on schizophrenia (SCZ) have suggested that proteins of the postsynaptic density of excitatory synapses have a role in its etiology. (nature.com)
Rational genetic engineering to enhance biotherapeutic proteins has become a reality catalyzed by publication of the genome sequences of multiple Chinese hamster ovary (CHO) cell lines. (bioprocessintl.com)
Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
Genetic engineering is the altering of the genetic material of living cells in order to make them capable of producing new substances or performing new functions. (faqs.org)
Furthermore, because of the antigenic and genetic diversity of "Norwalk-like viruses"* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)

Furthermore, we have shown that the variant MDV genome containing the duck genome substitution is present in the parental Md11 population and has been maintained through several subsequent propagations of the virus on chicken cells. (usda.gov)

Genomic responses to drugs can be studied using experimental animals (such as laboratory rats or mice) or live cells in the laboratory before embarking on studies with humans. (americascupmagazine.com)

The plasmid Acts of the Apostless as a cloning vector, provides reproduction of cloned cistron inside the host cell. (free-essays.us)
In transduction, the recombinant molecule is combined with phage caput and tail proteins from one cell to another utilizing bacteriophage vector. (free-essays.us)
Novagen's pTriEx-1 vector contains bacterial, insect, and mammalian promoters. (the-scientist.com)
Until recently, testing of multiple systems was time consuming, involving cloning and verification of recombinants in each system-specific vector. (the-scientist.com)
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. (gomolecular.pk)
Once the target DNA has been successfully ligated into the plasmid vector, the plasmid must be transferred into the host cell for replication and cloning. (igem.org)

Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)

Further, we show Rv1987 has moon lighting functions in M. tuberculosis pathobiology signifying roles of bacterial cellulose binding clusters in infections. (bvsalud.org)
Activation of the complement cascade always leads to the enzymatic cleavage of complement fraction C3, which is important in host defense to bacterial infections and phagocytosis. (medscape.com)
Although many people have no symptoms, some people have abnormal bleeding, recurring bacterial infections, and bone fractures due to severe osteoporosis. (msdmanuals.com)
Recurring bacterial infections resulting from inadequate production of normal antibodies may cause fever and chills. (msdmanuals.com)
These subtypes are clinically indistinguishable from one another, except for the fact that patients with GSD type Ib have altered neutrophil functions predisposing them to gram-positive bacterial infections. (medscape.com)
Liver disease, autoimmune disease, chronic viral or bacterial infections and various malignancies may cause a polyclonal rise in the gamma fraction (see Table 2 below). (medscape.com)

Keystone Symposia: Cell Biology of Virus Entry, Replication and Pathogenesis. (usda.gov)

Immunologic variations from the norm of WAS incorporate T-cell lymphopenia, flawed proliferative reaction to CD3 cross-linking, disabled counter acting agent reaction to polysaccharide antigens, flawed monocyte chemotaxis, anomalies of fortified dendritic cells, and an expanded lymphocyte apoptosis with age. (alliedacademies.org)
Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)
Both antibodies recognized human Langerhans cells, previously shown to express RANK, while dermal dendritic cells were poorly labeled. (cnrs.fr)

In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells. (elsevierpure.com)
This 1699 bp cDNA codes for a 41.6 kDa protein consisting of 372 deduced amino acid residues. (elsevierpure.com)
In COS-7 cells transiently transfected with the cDNA, a 250-fold increase was observed in specific activity toward 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid. (elsevierpure.com)

Moreover, treatment with imiquimod downregulated MHC class I molecules on tumor cells possibly resulting in a reduced binding affinity for inhibitory receptor NKG2A expressed on γδ T cells. (aacrjournals.org)
Thus, CD48 is a functionally relevant microbial receptor on mast cells that plays a role in triggering inflammation. (ox.ac.uk)
CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)
The TNF-family member Receptor Activator of NF-kappaB (RANK) is known for its role in bone homeostasis and is increasingly recognized as a central player in immune regulation and epithelial cell activation. (cnrs.fr)

Serum protein electrophoresis (SPEP) is an easy, inexpensive method of separating proteins based on their net charge, size, and shape. (medscape.com)
[ 1 ] Globulins comprise a much smaller fraction of the total serum protein but represent the primary focus of interpretation of serum protein electrophoresis. (medscape.com)
Various disease states or conditions alter the pattern of proteins in electrophoresis (see Table 1 below). (medscape.com)
Monoclonal pattern serum protein electrophoresis (SPEP). (medscape.com)

Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)

In addition, flagellin-specific CD4(+) T cells induced severe colitis when adoptively transferred into naive SCID mice. (nih.gov)
In vivo R12-31 agonist activity was demonstrated by its ability to induce the formation of intestinal villous microfold cells in mice. (cnrs.fr)

While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)

CD47 is a 50-kDa plasma membrane protein with an extracellular immunoglobulin variable region-like domain, a multiple membrane-spanning segment, and a short C-terminal cytoplasmic tail. (bdbiosciences.com)
Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding. (bdbiosciences.com)

The total protein recovered through standard bacterial cell lysis methods was heat treated and all Hisitidine-tagged recSSLPs were recovered using immobilized metal (nickel) affinity chromatography (IMAC). (uwyo.edu)
Here, we investigated the modulation of γδ T cell-mediated tumor cell lysis by TLR ligands. (aacrjournals.org)

Mast cells are well known for their harmful role in IgE-mediated hypersensitivity reactions, but their physiological role remains a mystery. (ox.ac.uk)
The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. (bvsalud.org)
Mast cells were found in 13 (61.9%) of the periapical cyst cases, located in the capsule of the lesion. (bvsalud.org)
In the periapical granuloma cases, mast cells were found in eight cases (50%), located in the granulation tissue. (bvsalud.org)
Mast cells were detected in both cysts and periapical granuloma, located in the capsule and granulation tissue, respectively. (bvsalud.org)
Mast cells were more prevalent in periapical cysts than in periapical granuloma. (bvsalud.org)
Mast cells. (bvsalud.org)
The exact mechanism associated with this growth and expansion remains unclear, although it is known that several types of cells, including mast cells, do play a role in such phenomena 4 . (bvsalud.org)
Mast cells are cells that reside in the connective tissue and contain a large number of granules, rich in histamine, heparin, chymase, serotonin, and also cytokines. (bvsalud.org)

additionally, a superfolder green fluorescent protein (sfGFP) was used as a reporter protein. (biomedcentral.com)
With a high-speed FACS sorter, highly fluorescent cells were enriched, and after two rounds of sorting, single clone exhibiting the highest level of sfGFP was isolated. (biomedcentral.com)

What is now clear is that certain membrane lipids can function as messenger signals that regulate cell growth, proliferation, inflammation, and programmed cell death processes, while other membrane lipids appear to cluster together in bilayers to form microdomains that regulate the spatial distribution and lateral interactions of membrane proteins. (umn.edu)
co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

Research in bacterial population genetics has increased in the last 10 years. (cdc.gov)
However, the reader should recognize that bacterial population genetics is a discipline separate and distinct from the study of the molecular epidemiology of infectious agents. (cdc.gov)

The dental environment as well as the BRL-15572 microorganisms it harbors, besides playing a job in bacterial endocarditis, have already been implicated in other styles of cardiovascular disease (6). (careersfromscience.org)

Therapeutic monoclonal antibodies (MAbs) mostly are manufactured using bioengineered mammalian cells cultured in a bioreactor for two to three weeks. (bioprocessintl.com)
The miap301 monoclonal antibody specifically binds to the extracellular domain of CD47, also known as Integrin-Associated Protein (IAP). (bdbiosciences.com)
Because this antibody is made by a single clone, it is called a monoclonal antibody and also is known as the M-protein. (msdmanuals.com)
A dense narrow band that is composed of a single class of immunoglobulins secreted by an abnormally expanded clone of plasma cells is known as M-protein (paraprotein, monoclonal protein or M-component). (medscape.com)

The discovery of these membrane lipid functions underscores why cell membranes so often come under direct attack during cancer and infectious disease. (umn.edu)
Our research focuses on membrane lipids known as sphingolipids and on proteins that interact in non-enzymatic fashion with sphingolipids. (umn.edu)
We have since focused our efforts on specific proteins that can bind and transfer sphingolipids between membrane surfaces and that may help formation and maintain 'raft' microdomains in cells. (umn.edu)
We have identified CD48, a glycosylphosphatidylinositol-anchored molecule, to be the complementary FimH-binding moiety in rodent mast cell membrane fractions. (ox.ac.uk)

Biofilms contain a small reversible subpopulation of so-called persister cells that adopt a slow- or nongrowing lifestyle through the emergence of small colony variants and are highly tolerant to extracellular stresses, such as antibiotic treatment. (medscape.com)
Some individuals focus on reproduction to expand the colony, while others specialize in construction, oozing polysaccharides and proteins that make up the extracellular matrix. (the-scientist.com)

Amino acid sequence homology comparison of the NDK of Mycobacterium smegmatis (MsmNDK) with the NDKs of other bacterial genera showed the presence of H 117 GSD motif. (openbiochemistryjournal.com)

While the recombinant wild type MsmNDK showed the NTPase, NTP synthesising, and autophosphorylation activities, the H117Q mutation abolished the biochemical activities of the recombinant MsmNDK-H117Q mutant protein in vitro . (openbiochemistryjournal.com)

Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Antibodies and cell lines. (jneurosci.org)

These properties lay in the presence of key structural amino acid sequences in these highly repetitive silk proteins. (uwyo.edu)
Mouse brain sialidase has two amino acid sequence motifs common to bacterial sialidases: the 'F/YRIP' motif and the 'Asp-box' motif. (elsevierpure.com)

In classic WAS, cruel platelet volume is 3.8 to 5.0 fL compared with 7.0 to 10.5 fL in sound subjects.4 In common, influenced patients illustrate both cellular and humoral immunodeficiency driving to repetitive bacterial, viral, and parasitic contaminations. (alliedacademies.org)

We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
NDKs from bacterial and other systems contain the conserved HGSD motif, where the His residue is required for the biochemical activities, namely the NTPase (AT-Pase and GTPase), NTP synthesising, and autophosphorylation activities of the enzyme. (openbiochemistryjournal.com)
A complete bacterial cell enzyme-linked immunosorbent assay (BactELISA) (12) aswell as traditional ELISAs using proteins had been used to identify proteins present either within the bacterial cell surface area or in proteins extracts. (careersfromscience.org)
2000. A metabolite of methoxychlor, 2,2-bis( p hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat leydig cells through suppression of steady-state messenger ribonucleic acid levels of the cholesterol side-chain cleavage enzyme. (cdc.gov)

The individual protein subunit of the virus capsid and formed in repetitive sequence. (innvista.com)

Cell cultures derived from mammalian and bacterial cell lines are the conventional production systems in bioprocessing. (bioprocessintl.com)
Media for mammalian cultures in particular are notoriously expensive, and traditional cell cultures can be highly sensitive to growing conditions. (bioprocessintl.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)

B cells One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)

The interaction of CD54 and the corresponding ligand CD11a/CD18 expressed on γδ T cells is responsible for triggering effector function in γδ T cells. (aacrjournals.org)

With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)

The work is either for the preparation of protein for crystallization and structural analysis or for functional studies by mutagenesis. (uni-leipzig.de)

Scientists found that the precise arrangement (or sequence) of nitrogen bases A, C, G. and T on the DNA strand is the recipe that codes for the manufacture of specific chemical compounds, namely proteins. (faqs.org)
The sequence acts, therefore, as an "instruction manual" that directs all cell functions. (faqs.org)
DNA in the cells of the islets of Langerhans in the pancreas would normally contain that base sequence since the islets are the regions in which insulin is produced in mammals. (faqs.org)
There was conservation of alpha chain usage, since all clones expressed a member of the V alpha 1 family, but again CDR3 sequence conservation was not apparent. (ox.ac.uk)
Similarity searches of the nonredundant GenBank peptide sequence database by the PSI-BLAST program identified rat, hamster, human, and bacterial sialidases homologous to this mouse brain sialidase. (elsevierpure.com)

Spread cell solution uniformly over the agar plate using the "hockey stick," then before discarding, spread residual solution on the "stick" over a second plate to get more a more sparse colony distribution. (igem.org)

A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Opsonized immune complexes (coated by C3b and C4b) bind to CR1, mostly on red blood cells, and are cleared through the liver where they can be transferred to CR3-bearing phagocytes and endocytosed. (medscape.com)

These sphingolipid transfer proteins were initially identified as glycolipid transfer proteins (GLTPs) due to their specificity for transferring glycosphingolipids between membranes. (umn.edu)
Subtle differences in epitope specificity were detected for two clones with differing TcR. (ox.ac.uk)
Once more, T cell clones with identical alpha and beta TcR chains were obtained from the separate cloning procedures, suggesting oligoclonalty of T cells with this defined specificity in the patient's SF. (ox.ac.uk)

The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)

Eukaryotic cells, including human cells, surround themselves with bilayer membranes, i.e. thin, flexible layers that are only two molecules thick. (umn.edu)
The field of transcriptomics is the science of the entire collection of mRNA molecules produced by cells. (americascupmagazine.com)
They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

The students know the structure and function of proteins and enzymes, as well as the role of metal ions in biological systems. (uni-leipzig.de)
But once construction progresses, some cells will switch to producing spores or useful enzymes. (the-scientist.com)

If the bait and prey proteins indeed interact then the two factors fused to these two proteins are also brought into proximity with each other. (genomicglossaries.com)

IL-17a-producing gammadeltaT cells and NKG2D signaling mediate bacterial endotoxin-induced neonatal lung injury: implications for bronchopulmonary dysplasia. (jax.org)
Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from. (biosave.com)

Carcinogenesis is the development of cancer caused by the actions of certain chemicals, viruses and unknown factors on primarily normal cells. (innvista.com)
However, factors that regulate delivery of Na v 1.3 to the cell surface are not known. (jneurosci.org)

Insome cases, a single misspelled base is sufficient to cause a disease, suchas sickle cell anemia. (faqs.org)

Anatomy23

Organisms36

Diseases1

Chemicals and Drugs91

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes68

Disciplines and Occupations3

Information Science4

Health Care5

Recombinant Proteins4

Plasmids4

Genes17

Gene14

Escherichia5

Expression21

Peptides3

Vectors2

Coli2

Receptors5

Adhesion3

Bacteria6

Signal Transduction1

Artificial chromosome1

Infection8

Encodes2

Transfection1

Strains2

Genetic10

Genome1

Humans1

Vector6

Antigen-Prese1

Infections6

Replication1

Dendritic Cells4

CDNA3

Receptor4

Electrophoresis4

Fusion proteins1

Mice2

Epitopes1

Immunoglobulin2

Lysis2

Mast Cells9

Fluorescent2

Interactions3

Genetics2

Endocarditis1

Monoclonal4

Membrane4

Extracellular2

Mycobacterium1

Vitro1

Antibodies2

Amino acid2

Viral1

Enzyme4

Repetitive1

Mammalian3

Tissues1

Effector1

Mass spectrometry1

Structural1

Sequence5

Colony1

Bind2

Specificity3

Yeast1

Molecules4

Enzymes2

Interact1

Mediate2

Factors2

Sickle cell a1