• Until now, methods for controlling or programming bacterial cells involved transcriptional and post-transcriptional regulation. (sciencedaily.com)
  • In 80-90% of single-cell infections, phages enter the lysogenic cycle. (wikipedia.org)
  • It is the most important type of antibacterial agent for fighting bacterial infections , and antibiotic medications are widely used in the treatment and prevention of such infections. (wikipedia.org)
  • Antibiotics are used to treat or prevent bacterial infections, [29] and sometimes protozoan infections . (wikipedia.org)
  • Many viral infections are infectious, just as bacterial ones. (dhruthihospital.com)
  • Additionally, like bacterial infections, viral infections can spread through the consumption of contaminated food or drink as well as through insect bites. (dhruthihospital.com)
  • Bacterial infections are treated with antibiotics. (dhruthihospital.com)
  • Given the central role PGRP-LC plays in sensing systemic bacterial infections, steroid hormone regulation provides a significant degree of modulation on the ability of cells or animals to respond to bacterial infection. (biomedcentral.com)
  • Bacterial infections of the eye highlight a dilemma that is central to all immune-privileged sites. (harvard.edu)
  • Bacterial infections can affect pregnant individuals from implantation of the fertilized ovum through the time of delivery and peripartum period. (medscape.com)
  • The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. (usda.gov)
  • Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. (hindawi.com)
  • Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. (hindawi.com)
  • Although only an injection, Plazomicin (ZEMDRI) was fast-tracked by the FDA in 2012 because it does such a good job at treating serious bacterial infections. (hfma.org)
  • This article is about treatment of bacterial infection. (wikipedia.org)
  • To determine whether a bacterial or viral infection is the root of your disease, they might occasionally need to collect a sample for culture. (dhruthihospital.com)
  • A short period of desiccation-elevated peptidoglycan recognition protein-LC ( PGRP-LC ) expression in MTs, increased antimicrobial peptide ( AMP ) gene induction, and protected animals from bacterial infection. (biomedcentral.com)
  • We show that desiccation increased ecdysone synthesis in MTs, while inhibition of ecdysone synthesis or ecdysone receptor expression, specifically within MTs, prevented induction of PGRP-LC and reduced protection from bacterial infection. (biomedcentral.com)
  • Virologists, those devoting their lives to the study of virus biology, knew that the virus' genome had a gene encoding a coat protein that surrounded the virus' genetic material and that was essential to its infection of plant cells. (umn.edu)
  • ApV association with the ER initiated early and continued throughout the infection cycle. (usda.gov)
  • An antibiotic is a type of medicine used to treat bacterial infection . (mdwiki.org)
  • The Raman spectroscopy-based method enables early detection and quantification of pathogens in plants, to enhance plant disease management. (mit.edu)
  • Anaplasma phagocytophilum and Anaplasma marginale are closely related tick-transmitted obligate intracellular bacterial pathogens that cause debilitating and potentially fatal diseases. (usda.gov)
  • While both of these pathogens cause different diseases and infect different cells in the mammalian host, they share a similar life cycle within the tick. (usda.gov)
  • A better understanding of the means by which these pathogens are able to colonize and grow within the tick may lead to improved methods for preventing tick transmission. (usda.gov)
  • Upon entrance into the tick, both pathogens are obligated to establish a niche within the cells of the tick midgut. (usda.gov)
  • The studies reported in this paper demonstrate that both of these pathogens interact with the endoplasmic reticulum of the host cell. (usda.gov)
  • The agar well diffusion method was used to assess the antibacterial and antifungal activities of partially pure fungal taxol against human and fungal pathogens. (degruyter.com)
  • Moreover, growth inhibition experiments demonstrated the ability of the two strains alone and in combination to antagonize diverse Gram-negative and Gram-positive bacterial pathogens during sessile and planktonic growth. (frontiersin.org)
  • Many of the more than 30 bacterial, viral and parasitic pathogens that are transmissible sexually, including HIV, are transmitted predominantly through sexual intercourse. (who.int)
  • Role of microbes in biogeochemical cycles Physiological experiments and quantitative microbial analysis. (ntnu.edu)
  • Communicate the role of microorganisms in biogeochemical cycles, and human impact on the carbon and nitrogen cycles. (ntnu.edu)
  • Biogeochemical cycles. (lu.se)
  • In contrast to standard implementations of flux balance modeling, we made use of forced influx of carbon and light into the cell, to recapitulate overflow metabolism due to the decoupling of photosynthesis and carbon fixation from growth during nutrient limitation. (biorxiv.org)
  • Bacterial photosynthesis. (lu.se)
  • Testing the susceptibility of Staphylococcus aureus to antibiotics by the Kirby-Bauer disk diffusion method - antibiotics diffuse from antibiotic-containing disks and inhibit growth of S. aureus , resulting in a zone of inhibition. (wikipedia.org)
  • This extracellular matrix glues bacteria to surfaces and serves as a slimy shield, protecting cells in the interior from predators and antibiotics. (the-scientist.com)
  • What's more, oxygen depletion causes cells in the center to enter a hibernation-like state, making them tolerant to antibiotics that target metabolic processes. (the-scientist.com)
  • As a mutation in the TCA cycle was detected we tested the controversial hypothesis regarding the bacterial response to bactericidal antibiotics , put forward by Kohanski et al. (bvsalud.org)
  • Our results suggested a novel function of the conventional antibacterial peptides, and the anti-bacterial and anti-inflammatory properties of BMGlvA2 may allow it a potential substitute for conventionally used antibiotics or drugs. (biomedcentral.com)
  • Especially, they exhibit activity against some antibiotics-resistant bacterial species. (biomedcentral.com)
  • Since the bacteriophage's genetic information is incorporated into the bacteria's genetic information as a prophage, the bacteriophage replicates passively as the bacterium divides to form daughter bacteria cells. (wikipedia.org)
  • Bacteria sense these microenvironments and adapt by up or down regulating the expression of certain genes to influence different cell processes. (the-scientist.com)
  • To guard against unplanned side effects, the E. coli bacteria is also engineered to self-destruct when too many other bacterial cells are present. (bostonmagazine.com)
  • Examples of these functions might include interaction with host cells at particular times of day, such as timed release of neurotransmitters produced by the bacteria, or interactions with other bacteria such as antifungal production triggered by a meal rich in sugar. (sciencedaily.com)
  • Bacteria are microorganism bacteria with only one cell. (dhruthihospital.com)
  • This response is triggered by microbial cell walls, peptidoglycans from bacteria or beta-glucans from fungi, and is regulated by two nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathways, the Toll and Imd pathways [ 1 , 2 ]. (biomedcentral.com)
  • The Imd pathway responds to diaminopimelic acid-type peptidoglycan (DAP-type PGN) from the cell wall of Gram-negative and certain types of Gram-positive bacteria. (biomedcentral.com)
  • These bacteria were often absent from gram stain smears of patients with other bacterial morphotypes. (cdc.gov)
  • This direct antigen identification method is based on the random expression of viral polypeptides fused to chloramphenicol acetyltransferase (CAT) in bacteria, which are subsequently fed to major histocompatibility complex class II+ antigen-presenting cells and probed with antigen-specific T cells. (nih.gov)
  • Bacterial endotoxins, which are located on the outer cell walls of gram-negative bacteria, are the most common and dangerous pyrogens. (verifiedmarketresearch.com)
  • The cell cycle and cellular differentiation in bacteria. (lu.se)
  • a method of estimating the number of bacteria per unit sample. (cdc.gov)
  • Cytotoxin production by other bacteria (ie, Shigella dysenteriae, Vibrio parahaemolyticus, Clostridium difficile, enterohemorrhagic E coli ) results in mucosal cell destruction that leads to bloody stools with inflammatory cells. (medscape.com)
  • The cloning of maize PPDK regulatory protein (PDRP) revealed the regulatory protein had been sequenced from more than 200 bacterial species and had been identified as DUF299 (domain of unknown function). (scirp.org)
  • Evidence suggests that both species may also infect endothelial cells in vivo. (usda.gov)
  • The species composition of the microflora was determined by amplification and sequencing of the 16S bacterial gene. (matis.is)
  • A total of 528 sequences were sequenced showing 174 different 16S bacterial sequences in the samples and 52% were found to belong to previously unknown bacterial species. (matis.is)
  • Similarly, Salmonella and Yersinia species also invade cells but do not cause cell death. (medscape.com)
  • Lysogeny, or the lysogenic cycle, is one of two cycles of viral reproduction (the lytic cycle being the other). (wikipedia.org)
  • The genetic material of the bacteriophage, called a prophage, can be transmitted to daughter cells at each subsequent cell division, and later events (such as UV radiation or the presence of certain chemicals) can release it, causing proliferation of new phages via the lytic cycle. (wikipedia.org)
  • The difference between lysogenic and lytic cycles is that, in lysogenic cycles, the spread of the viral DNA occurs through the usual prokaryotic reproduction, whereas a lytic cycle is more immediate in that it results in many copies of the virus being created very quickly and the cell is destroyed. (wikipedia.org)
  • One key difference between the lytic cycle and the lysogenic cycle is that the latter does not lyse the host cell straight away. (wikipedia.org)
  • Phages that replicate only via the lytic cycle are known as virulent phages while phages that replicate using both lytic and lysogenic cycles are known as temperate phages. (wikipedia.org)
  • The daughter cells can continue to replicate with the prophage present or the prophage can exit the bacterial chromosome to initiate the lytic cycle. (wikipedia.org)
  • In the lysogenic cycle the host DNA is not hydrolyzed but in the lytic cycle the host DNA is hydrolyzed in the lytic phase. (wikipedia.org)
  • A phage may decide to exit the chromosome and enter the lytic cycle if it is exposed to DNA-damaging agents, such as UV radiation and chemicals. (wikipedia.org)
  • However, phages may also re-enter the lytic cycle spontaneously. (wikipedia.org)
  • In the other 10-20%, phages enter the lytic cycle. (wikipedia.org)
  • Since phages that enter the lytic cycle kill the host bacterial cells, plaques will appear clear (photo A). The plaques may also appear to have a halo-like ring around the edge, indicating that these cells were not fully lysed. (wikipedia.org)
  • If prophages become active, they can exit the bacterial chromosome and enter the lytic cycle, where they undergo DNA copying, protein synthesis, phage assembly, and lysis. (wikipedia.org)
  • Using this approach, the BALF4 and BNRF1 proteins were identified as targets of the EBV-specific T-helper-cell response, demonstrating that lytic cycle antigens are a relevant component of the EBV-specific TH-cell response. (nih.gov)
  • Here, we describe a simple procedure that permits the identification of viral T-helper (TH)-cell antigens and epitopes. (nih.gov)
  • To introduce the engineered viral gene into the plant's chromosome without crossing, horticulturist John Sanford invented a means of introducing genes into plant cells by literally shooting the gene into the nucleus of a cell. (umn.edu)
  • But once construction progresses, some cells will switch to producing spores or useful enzymes. (the-scientist.com)
  • This demonstration illustrates one method of plant disease spread, water splashing of fungal spores or bacterial cells. (apsnet.org)
  • Instead, coffee grounds are substituted for spores or cells and polymer balls are used for water drops. (apsnet.org)
  • Pathogen spread is the result of velocity (magnitude and direction) of each polymer ball at the point of impact and the amount of coffee grounds (spores or cells) it hits. (apsnet.org)
  • a device to monitor the sterilization process that consists of a standardized population bacterial spores known to be resistant to the mode of sterilization being monitored. (cdc.gov)
  • Based on the significant antibacterial activity, the sorting of bacterial cells against the compounds (MID6 and MID9) was analyzed by flow cytometry. (degruyter.com)
  • In this study, seawater samples around the country were gathered for analysis concentration and diversity of microorganisms using flow ‐ cytometry and molecular methods. (matis.is)
  • Insertion of proteins into the bacterial outer membrane is mediated by a dedicated chaperone machinery. (unibas.ch)
  • We then analysed the proteomes of organoids with differential responses to 5-Fluorouracil and Oxaliplatin treatment using mass spectrometry, finding proteins associated with a mitotic cell cycle theme to be of greater abundance in relatively 5-FU/Oxal resistant samples compared to sensitive ones. (edu.au)
  • In this study, samples of the sea around the country were collected for analysis of microbial concentrations by flow cytometry and their diversity using molecular biological methods. (matis.is)
  • The microbial concentration was highest in Selvogsbanki, where the total count was about 1.6 million cells / mL of seawater. (matis.is)
  • Microbial concentrations were highest at Selvogsbanki, where the total counts were around 1.6 million cells / ml. (matis.is)
  • Microbial suspensions obtained from Hadza or Italian stools were first evaluated for structural integrity by high resolution-scanning electron microscopy and co-incubated with HT29 cell monolayers. (frontiersin.org)
  • Thus, it can replicate within these cells since it is protected from the humoral antibodies and antibiotic treatments [ 4 , 5 ]. (biomedcentral.com)
  • Describe the structure of an eukaryotic cell, and the central processes in cell division. (ntnu.edu)
  • 20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
  • Bacterial cytotoxins: targeting eukaryotic switches. (xenbase.org)
  • The field was firmly established in 2000, with the description of synthetic biological circuits in which parts of a cell are designed to perform functions, similar to the way an electronic circuit works. (sciencedaily.com)
  • The fluorescence images were pre-processed using a Gaussian of Laplacian filter to improve MiSiC prediction (describe in Materials and methods). (elifesciences.org)
  • UC San Diego researchers led by Jeff Hasty, a professor of bioengineering and biology and member of the UC San Diego Center for Microbiome Innovation, describe a new method, which involves cutting circular pieces of bacterial DNA called plasmids, effectively destroying the DNA and turning off regulation. (sciencedaily.com)
  • These discoveries help describe mechanisms by which metastatic colorectal cancer cells can evade the cytotoxic effects of chemotherapeutic drugs. (edu.au)
  • Enterocyte invasion is the preferred method by which microbes such as Shigella and Campylobacter organisms and enteroinvasive E coli cause destruction and inflammatory diarrhea. (medscape.com)
  • There are 3 types of parasites: Single-cell organisms (protozoa, microsporidia) Multicellular. (msdmanuals.com)
  • citation needed] It is sometimes possible to detect which cycle a phage enters by looking at the plaque morphology in bacterial plate culture. (wikipedia.org)
  • The oil immersion lens (x1000) was switched and between 10 and 20 representative fields was examined to observe cell morphology and gram reaction. (cdc.gov)
  • The researchers reasoned that the method could be used to regulate an entire suite of genes and promoters, and tested their idea using a previously constructed circuit to produce sustained cycling of DNA plasmid concentration across a colony of E. coli cells. (sciencedaily.com)
  • Because AHL is small enough to diffuse between cells and turn on the promoter in neighboring cells, the genes activated by it would also be produced in high amounts, leading to a phenomenon known as quorum sensing. (sciencedaily.com)
  • In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
  • This method identifies cooperative module pairs that significantly influence a cellular process and the correlated genes and interactions that are essential to that process. (biomedcentral.com)
  • 11. Melchers, F.: Synthesis, transport and secretion of immunoglobulin in lymphoid cells. (uni-mainz.de)
  • Alternative paclitaxel production methods, such as chemical synthesis and Taxus sp. (degruyter.com)
  • For instance the AIDS viruses can either infect humans (or some other primates) lytically, or lay dormant (lysogenic) as part of the infected cells' genome, keeping the ability to return to lysis at a later time. (wikipedia.org)
  • Properties of bacterial viruses and their reproduction. (ntnu.edu)
  • Your body's cells are susceptible to invasion by viruses, which feed off the components of your cells to grow and spread. (dhruthihospital.com)
  • Even some viruses' life cycles include killing host cells. (dhruthihospital.com)
  • Some rhabditiform larvae convert within the intestine to infectious filariform larvae that immediately reenter the bowel wall, short-circuiting the life cycle (internal autoinfection). (msdmanuals.com)
  • Previous studies indicated that endotoxins usually caused intestinal mucosa inflammation and destruction of tight junction integrity and epithelial cell apoptosis [ 4 ], which subsequently led to disruption of intestinal homeostasis and damage of the intestinal barrier functions. (biomedcentral.com)
  • This enrichment scales with the number of PCR cycles performed, and is presumably due to preferential amplification of short inserts. (peerj.com)
  • We found that a pipeline combining read deduplication and an assembly algorithm originally designed to recover genomes from libraries generated after whole genome amplification (single-cell SPAdes) frequently improved assembly of contigs ≥10 kb by 10 to 100-fold for low input metagenomes. (peerj.com)
  • After a series of successive cycles of amplification, the presence of double stranded DNA product was visualized on agarose gels stained with ethidium bromide. (cdc.gov)
  • In this work, we propose a new approach to identifying condition-related cooperative interactions, and importantly, we establish a cell cycle-specific cooperation module network. (biomedcentral.com)
  • Since these early inventions, Hasty and his team have shown how engineered cellular oscillations can be synchronized within a bacterial colony using plasmids, synthetically designed by the researchers themselves. (sciencedaily.com)
  • Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducible, and a labor- and cost-effective approach for rapid production of bacterial lysates for cell-free gene expression. (arborbiosci.com)
  • Originally demonstrated to control the dynamics of the F- actin cytoskeleton , Rho GTPases have been implicated in many basic cellular processes that influence cell proliferation, differentiation, motility, adhesion, survival, or secretion. (xenbase.org)
  • These results provide a global view of the cell cycle and the method can be used to discover the dynamic coordination properties of functional components in other cellular processes. (biomedcentral.com)
  • The induced dysbiosis was characterized by alterations in bacterial abundance, composition, and diversity in our animal models. (aacrjournals.org)
  • The researchers are reprogramming harmless strains of E. coli to destroy tumor cells. (bostonmagazine.com)
  • First, it can produce a molecule called hemolysin, which damages the tumor cells' essential membranes. (bostonmagazine.com)
  • Another method induces the tumor cells to undergo "programmed suicide. (bostonmagazine.com)
  • Mechanistic studies revealed that this progression was mediated by suppressed TNFα levels, both locally and systemically, resulting in reduced expression of tumor endothelial adhesion molecules, particularly intercellular adhesion molecule-1 (ICAM-1) and a subsequent decrease in the number of activated and effector CD8 + T cells in the tumor. (aacrjournals.org)
  • The tumor is ablated using small high-voltage pulses that induce slow cell death over time. (hfma.org)
  • The advantages of this type of ablation are it is very tissue selective, only destroying the cancer cells and preserving the vital structures that surround the tumor, and it is also very quick. (hfma.org)
  • These results are consistent with previous studies and demonstrate that our methodology is effective for studying cooperative mechanisms in the cell cycle. (biomedcentral.com)
  • Therefore, stomatal guard cells represent an ideal system to test whether GABA signalling occurs in plants. (nature.com)
  • More severe illness can result if either primary influenza pneumonia or secondary bacterial pneumonia occurs. (cdc.gov)
  • Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. (arborbiosci.com)
  • The autolysis approach can facilitate wider adoption of cell-free technology for cell-free gene expression as well as other synthetic biology and biotechnology applications, such as metabolic engineering, natural product biosynthesis, or proteomics. (arborbiosci.com)
  • The course is an optional second-cycle course for a degree of Bachelor or Master of Science in Biology. (lu.se)
  • b ) A set of annotated bright-field images of Escherichia coli and Myxococcus xanthus along with synthetic labeled data with additive Gaussian noise was used to generate a training dataset of input images, X, consisting of Shape Index Map of intensity images (at three scales) and segmented images, Y, consisting of contours ( Y1 ) and cell body ( Y2 ). (elifesciences.org)
  • Researchers have tested the E. coli method in mice, using it to battle a very aggressive form of colon cancer that had spread to the liver. (bostonmagazine.com)
  • Enterotoxigenic Escherichia coli (ETEC) is one of the leading bacterial causes of intestinal inflammation and diarrhea. (biomedcentral.com)
  • They also change the lining of the uterus (womb) to prevent pregnancy from developing and change the mucus at the cervix (opening of the uterus) to prevent sperm (male reproductive cells) from entering. (medlineplus.gov)
  • a bacterial induced inflammation of the lining of the heart and its valves. (cdc.gov)
  • In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
  • Other histopathology findings included mild necrosis of the epithelial cells of the renal tubules, congestion and occasional haemorrhages in the various tissues. (biomedcentral.com)
  • Institute for Cell and Molecular Biosciences of Newcastle University. (ncl.ac.uk)
  • Lysogeny is characterized by integration of the bacteriophage nucleic acid into the host bacterium's genome or formation of a circular replicon in the bacterial cytoplasm. (wikipedia.org)
  • GMO technology offers, for example, the possibility of engineering resistance to the corn rootworm by introducing a bacterial transgene into corn or the ability to test a gene from any source in a plant. (umn.edu)
  • Despite being highly cytotoxic, their clinical efficacy is limited by mechanisms of chemotherapeutic resistance within cancer cells, which contributes to poor patient outcome. (edu.au)
  • Background noise can lead to spurious cell detection by MiSiC. (elifesciences.org)
  • This effect is shown here: When an image with uniform and random intensity values is segmented with MiSiC with increasing smoothening (here using a gaussian blur filter), spurious cell detection becomes apparent. (elifesciences.org)
  • Detection methods of phages released from the lysogenic cycle include electron microscopy, DNA extraction, or propagation on sensitive strains. (wikipedia.org)
  • Kovács's team has found that in populations of the soil bacterium Bacillus subtilis , most microbes assume responsibility for matrix production during early development when they are little more than a throng of unconnected cells. (the-scientist.com)
  • The MTT assay was used to examine the cytotoxic effects of fungal taxol against the human liver cancer cell line HepG2. (degruyter.com)
  • In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)
  • As an intracellular parasite, the bacterium is able to manipulate the host's immune system and flourishes within the professional and non-professional phagocytic cells. (biomedcentral.com)
  • Finally, antigen identification does not require knowledge of the restriction element and often leads directly to the identification of the T-cell epitope. (nih.gov)
  • Fibroblastic reticular cells (FRCs) are known to inhabit T cell-rich areas of lymphoid organs where they function to coordinate T cell and dendritic cell interactions. (harvard.edu)
  • Beta globin is common to all mammalian cells and it is reasonable to expect that some human cells will be present in the sample. (cdc.gov)
  • In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). (usda.gov)
  • A significant decrease (0.05-0.5 μM) was observed in the cell viability and IC 50 dosage was fixed as 0.25 μM after 24 h of treatment. (degruyter.com)
  • Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability. (xenbase.org)
  • By means of a recently developed non-invasive ex vivo minimal model based on the interaction of the human enterocyte-like HT29 cell line and fecal slurries, we explored the enterocyte-associated microbiome of 21 Hadza hunter-gatherers and nine urban living Italians. (frontiersin.org)
  • The structure and function of prokaryotic cells. (lu.se)
  • In this thesis, we optimised a mass spectrometry sample preparation method to permit the proteomic analysis of metastatic colorectal cancer patient derived tumour organoids and their supernatants. (edu.au)
  • The carbohydrate composition of a myeloma protein from different subcellular fractions of plasma cells. (uni-mainz.de)
  • Cell division yields distinct progeny, a swarmer cell and a stalked cell. (harvard.edu)
  • A new method for controlling gene expression across bacterial colonies has been invented by researchers. (sciencedaily.com)
  • Researchers at the University of California San Diego have invented a new method for controlling gene expression across bacterial colonies. (sciencedaily.com)
  • The circuit works by using a small molecule, known as AHL, to coordinate gene expression across a colony of bacterial cells. (sciencedaily.com)
  • Using this method, robust and highly active bacterial cell lysates can be produced without specialized equipment at a wide range of scales, making cell-free gene expression easily and broadly accessible. (arborbiosci.com)
  • Laub MT, McAdams HH, Feldblyum T, Fraser CM, Shapiro L. Global analysis of the genetic network controlling a bacterial cell cycle. (harvard.edu)
  • This approach has also helped researchers understand natural "oscillators," such as our sleep-wake cycle. (sciencedaily.com)
  • Now, the team is adding a new tool to the Synthetic Biologist's toolbox -- a "master clock" of sorts that will allow researchers to coordinate subprocesses in bacterial cells. (sciencedaily.com)
  • Protein level research of these mechanisms has traditionally been limited in scope and often restricted to two-dimensional cell experimental models. (edu.au)
  • METHODS: Trends in 1-year net survival were estimated using the Pohar Perme estimator, controlling for background mortality with life tables of all-cause mortality rates by county of residence, single year of age, sex, and race for each year 2001-2013. (cdc.gov)
  • Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. (harvard.edu)
  • If the female is interrupted while feeding, it may seek another host on which to feed, leading to multiple transmissions during a feeding cycle. (medscape.com)
  • In this condition the bacterium continues to live and reproduce normally, while the bacteriophage lies in a dormant state in the host cell. (wikipedia.org)
  • In its inactive form, a prophage gets passed on each time the host cell divides. (wikipedia.org)
  • By coupling sequencing and pairwise competition experiments, we demonstrated that the first mutation λ evolved en route to the innovation would only evolve in the presence of the ancestral host, whereas later steps in λ's evolution required the shift to a resistant host. (elifesciences.org)
  • Here, we demonstrate that the ApV and AmV extensively interact with the host endoplasmic reticulum (ER) in endothelial, myeloid, and/or tick cells. (usda.gov)
  • Climate change in the environment is usually not rapid, allowing cells to acclimatize. (who.int)