Bacterial attachment site. Medical search. Frequent questions

Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)
3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)
Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
Engineer microbial biofilms that physically modify surfaces to decrease bacterial attachment sites and prevent bacterial adhesion. (ebrc.org)
Engineer microbial biofilms that degrade bacterial holdfast structures to prevent "primary surface colonizers" from attaching and starting the biofilm formation process. (ebrc.org)

In both EHEC and EPEC, the proteins that mediate this attachment are encoded in a chromosomal pathogenicity island called the "locus of enterocyte effacement" (LEE) ( 3 ). (cdc.gov)
The covalent attachment of ubiquitin (Ub) to target proteins represents one of the most versatile and common post-translational modifications in eukaryotic cells. (baypat.de)
We present a new method allowing for the site-specific ubiquitylation and SUMOylation of target proteins - both in vitro and in cellulo* . (baypat.de)
Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
The interaction of LF with microbial surfaces-OMPs of Gram-negative bacteria in particular-has led to other antimicrobial mechanisms, such as the inhibition of microbial attachment to sub-epithelial matrix proteins and detachment of bacteria from mucosal surfaces. (ift.org)
A lack of these proteins impairs the body's immune response to bacteria, leading to recurrent bacterial infections. (medlineplus.gov)
Mutations in another gene involved in the attachment of peptides to MHC class I proteins very rarely cause BLS I. (medlineplus.gov)

This attachment allows for colonization and competition with normal host flora. (medscape.com)
The colonization of a surface begins with EPS production by the initial bacterial colonizers through the formation of weak, reversible bonds called van der Waals forces and production of transparent exopolymer particles and their precursors by macroalgae that set the basis for the first bacterial colonizers settlement [ 1 ]. (intechopen.com)
Early onset colonization of an implant is often caused by introduction of bacteria into the surgical site during placement of the implant, however, another source of bacteria is the blood. (biomedcentral.com)
Implanted medical devices, such as intramedullary nails or total joint prostheses, encourage bacterial colonization at the site of implantation and are therefore highly susceptible to infection [ 7 ]. (biomedcentral.com)

The main function of the layer is protection from mechanical stress, dehydration, and bacterial infections. (wikipedia.org)
Infections of implanted orthopedic devices can lead to implant failure, resulting in additional antibiotic therapies and possible surgical intervention, removal of the implant and subsequent revision of the implant site. (biomedcentral.com)
Ehrlichiosis and Anaplasmosis Ehrlichiosis and anaplasmosis are tick-borne bacterial infections that cause fever, chills, muscle aches, headache, a general feeling of illness (malaise), and sometimes a rash. (msdmanuals.com)
Starting in childhood, most people with BLS I develop recurrent bacterial infections in the lungs and airways ( respiratory tract ). (medlineplus.gov)

Tannerella forsythia is a bacterial pathogen involved in periodontal disease. (elsevierpure.com)

The explosion of multidrug and pandrug resistant strains of a diversity of important bacterial pathogens, including ESBL (extended spectrum beta-lactamase), methicillin/vancomycin resistant Staphylococcus aureus (MRSA/VRSA), and pandrug resistant Acinetobacter baumannii ( Fair and Tor, 2014 ), seen over the last decade is a further indication that our current attempts to dampen the spread of resistance is not as efficient as needed. (frontiersin.org)

In particular, surface modifications that physically create rational surface topographies have attracted attention in recent years, and have shown to inhibit bacterial attachment and biofilm growth without the use of antimicrobials. (rsc.org)
Considered generally recognized as safe (GRAS) by the Food and Drug Administration and recently approved by the U.S. Dept. of Agriculture for use on fresh beef, ALF can be sprayed onto carcasses to help prevent bacterial contamination during processing or can be applied to a subprimal or finished beef surface prior to final packaging to inhibit bacterial growth and extend shelf life. (ift.org)

The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
In addition to bacterial organisms, oral microorganisms can include fungal, protozoal, and viral species. (medscape.com)

This process of inducing virulence in an otherwise harmless bacterium through prophage infection and integration of the prophage genome into the bacterial host genome is called lysogenic conversion 8 . (nature.com)
The integration of phage λ takes place at a special attachment site in the bacterial genome, called att λ . (wikidoc.org)
The new tool combines the precise targeting of CRISPR-Cas9, a set of molecules originally derived from bacterial defense systems, with enzymes called integrases, which viruses use to insert their own genetic material into a bacterial genome. (sflorg.com)
These enzymes target specific genome sequences known as attachment sites, which function as "landing pads. (sflorg.com)
This allows them to target any site in the genome for insertion of the landing site, which contains 46 DNA base pairs. (sflorg.com)
Once the landing site is incorporated, the integrase can come along and insert its much larger DNA payload into the genome at that site. (sflorg.com)
Whole-genome and site-specific fluorescent DNA labeling in tethered cells indicates that global nucleoid structure and chromosome organization are disrupted. (nih.gov)
Hybridization of the gRNA to the target site localizes Cas9, which then cuts the target site in the genome. (vectorbuilder.cn)
The φC31 integrase mediates irreversible recombination between attB and attP sites, resulting in the linearization and integration of the pUASTB vector into the host genome. (vectorbuilder.cn)
Additionally, φC31-based insertion is site-specific, generally occurring only at attP sites, and not elsewhere in the genome. (vectorbuilder.cn)
For this reason, the attB vector system is designed to be used with Drosophila lines carrying attP "landing sites" within their genome. (vectorbuilder.cn)

Adhesins enable GABHS attachment at sites such as the pharynx. (medscape.com)
Intracellular events, such as blocking of microbial attachment factors such as fimbriae (hairlike structures) and other adhesins (putative receptors), have been observed (Naidu and Bidlack, 1998). (ift.org)

The effects the other bacteria have on Campylobacter survival, aggregation (auto-aggregation and co-aggregation), attachment and biofilm development on poultry skin. (usda.gov)

However, the underlying mechanisms linking surface topography to the inhibition of microbial attachment and growth still remain unclear. (rsc.org)

Having bacterial vaginosis can be frustrating and miserable. (wikidot.com)
Some women might not know the different symptoms of bacterial vaginosis. (wikidot.com)
If you're noticing that you have a fishy smell, especially during sex, you more than likely have bacterial vaginosis. (wikidot.com)
They're not sure if it's bacterial vaginosis or yeast infection. (wikidot.com)
A few other bacterial vaginosis symptoms are irritation, burning, and discomfort to name a few. (wikidot.com)
If you are having one or more of the symptoms above, you might have bacterial vaginosis. (wikidot.com)

Bacteria often require a substrate to attach themselves to before they can multiply, and the attachment can be host specific or site specific. (who.int)
Bacterial attachment is favoured on recessed portions of patterned surfaces, and bacteria tend to attach preferentially to patterns in the micro or nanometre range rather than to smooth surfaces. (rsc.org)
The green sulfur bacterial epibionts are related to more 'typical' green sulfur bacteria, complete with chlorosomes and an autotrophic reverse TCA pathway to assimilate carbon. (asmblog.org)
The disease presents as bacterial abscesses forming adjacent to the physis in the metaphyseal region of the bone, where bacteria can accumulate in the capillary beds (sinuses) [ 2 ]. (biomedcentral.com)
We examine the efficiency to capture and detect target bacteria in suspension using immunomagnetic particles in two different attachment modes namely, random assembly and oriented assembly of antibodies. (edu.au)
In a second series of experiments, attachment of non-typable H. influenzae to epithelial cells collected from RSV infected cotton rats at the time of maximum virus replication was not different from controls (57.4 ± 18.3 vs 52.0 ± 24.3 bacteria per 50 cells). (utmb.edu)
Activated form of natural antimicrobial improves safety of beef and poultry by preventing attachment and growth of bacteria. (ift.org)

Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)
The putative phage attachment site. (phaster.ca)
The bacteriophage φC31 encodes an integrase that mediates efficient, sequence-specific recombination between phage attachment sites (called attP) and bacterial attachment sites (called attB). (vectorbuilder.cn)

Threadlike structures ("bacterial nanotubes") protruded from the majority of bacterial cells and appeared to link cells directly with the nanopillars. (rsc.org)
Periodontitis is a common chronic bacterial infection of the supporting structures of the teeth. (medscape.com)
Chronic periodontitis is a bacterial-induced chronic inflammation within the structures that support the teeth, resulting in progressive attachment and bone loss ( 1 ). (spandidos-publications.com)

The common organosilane aminopropyltriethoxy silane (APTES) under reflux conditions amine functionalizes γFe 2 O 3 nanoparticles enabling covalent attachment of anti-Salmonella polysera. (edu.au)
Thus we demonstrate IMS-PCR with improved surface chemistry and covalent attachment of antibody was sensitive to 10 6 and 10 7 cells/ml respectively. (edu.au)
Thus we demonstrate IMS-PCR with improved surface chemistry and covalent attachment of antibody to be rapid, specific and cost effective detection method for Salmonella species in clinical isolates. (edu.au)

In Vibrio cholerae , infection by a bacterial virus (bacteriophage) results in the conversion of non-pathogenic strains to pathogenic strains and this can lead to cholera pandemics. (nature.com)
Increased susceptibility to infection from S. aureus can be attributed to the mechanism of bacterial attachment to an implant and the deactivation of local granulocytes upon introduction of the implant itself [ 1 ]. (biomedcentral.com)
Along with any bacterial contamination associated with surgery, there is a threat of infection throughout the life of the implant from hematogenous sources. (biomedcentral.com)
These factors support anecdotal evidence that Borrelia infection can occur in humans within a short time after tick attachment. (google.com)
Foci of infection in the oral cavity arising from chronic periodontitis or chronic periapical abscesses (ie, inflammation and abscess of the tissue attached to the apex of the root) may lead to subacute bacterial endocarditis (BE) and glomerulonephritis (GN). (medscape.com)

Moreover, several Lipoptena species have been associated with zoonotic bacterial agents such as Bartonella spp. (scielo.br)
29 The multiple barbs of the hypostome lock the tick into the epidermis and help prevent host activities, for example, grooming, from detaching the tick, and in some species, a cement plug is created to further ensure firm attachment. (google.com)
MY NOTE- An inflammatory response at the tick bite site (not an EM rash) could possibly indicate the inhibitors used to suppress the host immune response were not fully functioning, or in varying species of ticks are more/less viable than others? (google.com)
Using comparative sequence and structural analyses, we have identified homologs of PrtH in a number of bacterial and archaeal species. (elsevierpure.com)

Nonetheless, the lack of toxins leads to reduced bacterial replication in infected hosts. (bvsalud.org)

Hard-bodied ticks of the genus Ixodes also carry bacterial and parasitic diseases including: anaplasmosis, babesiosis, ehrlichiosis, rickettsiosis, and bartonellosis. (google.com)

The new tool, PASTE (Programmable Addition via Site-specific Targeting Elements), includes a Cas9 enzyme that cuts at a specific genomic site, guided by a strand of RNA that binds to that site. (sflorg.com)

Receptors on the bacterial cell surface which are recognized by BACTERIOPHAGES. (bvsalud.org)

The EspA organelle is used to transfer the translocated intimin receptor (Tir), which is then inserted into the host cell membrane, where it binds to intimin, a bacterial outer membrane protein ( 9 , 10 ) and triggers the host cytoskeletal events that lead to attaching/effacing lesion formation. (cdc.gov)
N binds to the two Nut sites, one in the N gene, and one in the cro gene. (wikidoc.org)

Here, we investigated bacterial attachment, cell alignment and biofilm formation of Pseudomonas aeruginosa on periodic nano-pillar surfaces with different pillar spacing. (rsc.org)

P transposase-mediated insertion occurs without any significant bias with respect to insertion site sequence. (vectorbuilder.cn)

The enzymes contain ~250-300 residues, which encode putative signal sequences and carbohydrate attachment sites. (embl.de)

To achieve this goal, they turned to a family of enzymes called integrases, which viruses called bacteriophages use to insert themselves into bacterial genomes. (sflorg.com)
In past work, scientists have found it challenging to develop these enzymes for human therapy because the landing pads are very specific, and it's difficult to reprogram integrases to target other sites. (sflorg.com)

In addition, a specific pathologic condition, such as periodontitis (ie, inflammation of the periodontal attachment of the teeth and the alveolar bone), may be present in the oral cavity. (medscape.com)
In total, 52 GCF samples from disease sites of patients with chronic periodontitis, prior to and following periodontal therapy, and ten non‑disease sites from non‑periodontitis subjects, were collected and cytokine concentrations were determined using a multiplex method. (spandidos-publications.com)
The results support the hypothesis that proinflammatory cytokines, including IL‑1α, IL‑1β and IL‑6, are likely to be involved in the pathogenesis of periodontitis and are good markers to evaluate the success of nonsurgical therapy in disease sites of patients with periodontitis. (spandidos-publications.com)

Professor Brun has done groundbreaking work to understand the mechanisms of bacterial attachment and biofilm formation. (harvard.edu)
Building on our previous research, we will investigate strain diversity and mechanisms of tolerance to stresses, including acid and exposure to antimicrobial compounds, as well as investigate factors affecting attachment and biofilm formation of Campylobacter. (usda.gov)
As a post-doctoral fellow with W. Wallace Cleland, I synthesized the novel compound, Cr(III)ATP, and showed it to be a useful paramagnetic, dead-end inhibitor for exploring the active site and kinetic mechanisms of enzymes that required ATP as one of their substrates. (nih.gov)

This process is responsible for the toxicity of some bacterial toxins (e.g., cholera and pertussis toxins). (embl.de)

Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)

Biopsies were collected from sites of tick attachment and examined for A. phagocytophilum and B. burgdorferi-specific DNA by PCR procedures. (auburn.edu)
Michael Cook reports- 'The claims that removal of ticks within 24 hours or 48 hours of attachment will effectively prevent LB are not supported by the published data, and the minimum tick attachment time for transmission of LB in humans has never been established. (google.com)
A newly released study by Michael Cook, Lyme borreliosis: a review of data on transmission time after tick attachment , who reviewed scientific literature published over the past century, indicates a tick can be attached for much less than 24/48 hours and still transmit Lyme disease, something many people bitten by ticks already, unfortunately, learned from experience. (google.com)
Tick attachment starts by inserting the saw-like chelicerae into the epidermis to create an entrance wound. (google.com)

The objective of conventional periodontal therapy is to eliminate the subgingival biofilm at sites affected by periodontal disease, which may be associated to progressive destruction of supportive periodontal tissue 1 . (bvsalud.org)

More than half a century ago, Heukelekian and Heller [6] showed that at low nutrient concentrations substrate plays an important role in bacterial multiplication. (who.int)
Surface patterning is an important determinant of bacterial attachment. (rsc.org)

This website uses Google Analytics to collect anonymous information such as the number of visitors to the site, and the most popular pages. (ubi.se)

No reports exist on the structure, active site, and catalytic mechanism of PrtH. (elsevierpure.com)

To facilitate this flexibility, the Cas9 gene is cloned in a region bracketed by two P-element terminal repeats and near an attB recombination site. (vectorbuilder.cn)

Integration of attB into an attP position creates hybrid sites (called attL and attR), which are refractory to the φC31 integrase. (vectorbuilder.cn)

At the site of bacterial attachment, the host cell membrane forms a pedestal-like structure. (cdc.gov)
This time could vary with host animal size, the presence of competing ticks, or rejection of a site as unsuitable. (google.com)
After transferring to the host, the tick searches for a suitable attachment site. (google.com)
To utilize φC31 integrase-mediated insertion, the pUASTB plasmid and a φC31 integrase-expressing helper plasmid are co-introduced into host cells or embryos containing attP landing sites. (vectorbuilder.cn)

He is also one of the few microbiologists who has studied bacterial morphology and development from both cell biological and evolutionary perspectives. (harvard.edu)
We propose that bacterial nanotubes are produced to aid in cell-surface or cell-cell connections. (rsc.org)
The molecular level effects on motility, attachment/invasion of eukaryotic cell lines, and biofilm formation. (usda.gov)
As a graduate student with Julius Adler, I identified the basal body of the bacterial flagellum, develop methods for its purification, and elucidate its fine structure and specific attachments to the bacterial cell envelope. (nih.gov)

If you use toothpaste, use a fluoride-cost-free 1.The NHS will provide you with the dental care you call for to preserve your mouth, teeth click through the up coming Webpage and gums healthy and discomfort-free. (wikidot.com)

Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. (rsc.org)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)

In this pUASTB system, Cas9 gene is cloned downstream of an engineered, inducible promoter consisting of five tandemly arrayed GAL4 binding sites (5xUAS) and the hsp70 TATA box promoter. (vectorbuilder.cn)
a DNA region that includes DNase hypersensitive sites located 5′ to a gene or gene cluster, and which confers high-level, position-independent, and copy number-dependent expression on that gene or gene cluster. (insdc.org)

Untreated disease sites exhibited higher cytokine levels in the GCF when compared with the non‑disease sites. (spandidos-publications.com)
According to the study by Kinane et al , periodontal disease biomarkers can be grouped into several categories, namely, prognostic biomarkers that identify patients or sites most likely to respond to a specific treatment, and therapeutic biomarkers that provide a quantifiable measurement of the response to periodontal treatment ( 4 ). (spandidos-publications.com)
To the best of our knowledge, no biomarker has been shown to exhibit a prognostic value at the disease site or at the patient level. (spandidos-publications.com)
Do you have a heart problem (specifically congenital heart murmurs, heart valve problems, congenital heart disease, or bacterial endocarditis)? (cdc.gov)

Periodontal parameters, including bleeding on probing, probing pocket depth and the clinical attachment level, in all the sites were recorded. (spandidos-publications.com)
Clinical attachment level (CAL), probing depth (PD) and gingival margin level (GML) were assessed at baseline, and after 3 and 6 months, using a manual probe (PCP-15, Hu Friedy). (bvsalud.org)

In an attempt to determine the minimum transmission times of A. phagocytophilum and B burgdorferi, wild caught adult I. scapularis ticks were applied to cats and removed at 8, 16, 24, and 36 hours after attachment. (auburn.edu)
10 hours attachment time, they found that transmission could occur in as little as 10 minutes with fed ticks. (google.com)

Find support for a specific problem in the support section of our website. (mdpi.com)

Anatomy2

Organisms4

Diseases1

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Psychiatry and Psychology1

Phenomena and Processes7

Technology, Industry, Agriculture1

Information Science1

Biofilms5

Proteins8

Colonization4

Infections4

Pathogen1

Pathogens1

Inhibit bacterial2

Microorganisms2

Genome11

Adhesins2

Biofilm development1

Microbial1

Vaginosis6

Bacteria7

Phage3

Structures3

Covalent attachment3

Infection5

Species4

Replication1

Anaplasmosis1

Genomic1

Receptors1

Binds2

Surfaces1

Sequence1

Putative1

Integrases2

Periodontitis3

Mechanisms3

Toxins1

Protein1

Tick attachment4

Periodontal therapy1

Important2

Google Analytics1

Mechanism1

Recombination1

AttB1

Host4

Cell4

Teeth1

Surface2

Gene2

Disease4

Clinical2

Ticks2

Specific1