A cDNA encoding an infectious coronavirus RNA genome has been cloned as a bacterial artificial chromosome. (nih.gov)
Old World lupins constitute an interesting model for evolutionary research due to diversity in genome size and chromosome number, indicating evolutionary genome reorganization. (cas.cz)
It has been hypothesized that the polyploidization event which occurred in the common ancestor of the Fabaceae family was followed by a lineage-specific whole genome triplication (WGT) in the lupin clade, driving chromosome rearrangements. (cas.cz)
Oligonucleotide probes were designed to cover both arms of chromosome Lang06 of the L. angustifolius reference genome separately. (cas.cz)
The chromosome was chosen for the in-depth study due to observed structural variability among wild lupin species revealed by BAC-FISH and supplemented by in silico mapping of recently released lupin genome assemblies. (cas.cz)
The Genome Resource Center (GRC) provides expertise in BAC (bacterial artificial chromosomes) lib. (iths.org)
Horses from the Institute's experimental herd include the Thoroughbred stallion Bravo, the DNA donor for the equine Bacterial Artificial Chromosome library, and Twilight, the Thoroughbred mare whose DNA was the source for the horse genome sequence . (cornell.edu)
Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes. (biomedcentral.com)
Its genome has been sequenced and found to consist of a 4.65 Mb chromosome and three plasmids of 96.2 kb, 70.3 kb, and 9.6 kb. (damasgate.com)
We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophilagenome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. (umd.edu)
Cytogenetic manifestations of amplified DNA include self-replicating extrachromosomal elements called d ouble m inutes (DMs), amplified regions on a single chromosome ( h omogeneously s taining r egions, HSRs) or amplified regions distributed throughout the genome [ 5 ]. (biomedcentral.com)
BACs5
BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome using BAC arrays. (wikipedia.org)
Full length cDNA clone and direct sequencing of four BACs revealed an intact omTLR1 open reading frame (ORF) located on chromosome 14 and a second locus on chromosome 25 that contains a TLR1 pseudogene. (usda.gov)
Direct sequencing of two representative BACs revealed intact omTLR7 and omTLR8a1 open reading frames (ORFs) located on chromosome 3 and a second locus on chromosome 22 that contains an omTLR8a2 ORF and a putative TLR7 pseudogene. (usda.gov)
The main tools of GENSAT are bacterial artificial chromosomes (BACs), which are simple loops of bacterial DNA that reproduce outside the cell. (nih.gov)
A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. (wikipedia.org)
Following alkaline lysis of up to 500 ml culture (see flowchart " QIAGEN Plasmid Kit procedures. Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. "> QIAGEN Plasmid Kit procedures "), a unique integrated digestion step with ATP-dependent exonuclease provided with the kit, ensures selective removal of contaminating genomic DNA, as well as nicked or damaged construct DNA. (qiagen.com)
Using plasmid- and bacterial artificial chromosome-based transgenic reporter assays, we identified key cis -regulatory elements (CREs) and transcription factor inputs that regulate Sp-kirrelL , including direct, positive inputs from two key transcription factors in the skeletogenic GRN, Alx1 and Ets1. (elifesciences.org)
In addition, the circular chromosome con- tains three marker genes (M1, M2, and M3), which when expressed, allow selection of the cells carrying the plasmid and sites 1 and 2, which allow specific restriction endonucleases (q.v.), to break the mole- cule. (damasgate.com)
Our lab produces traditional transgenic mice by injecting plasmid or bacterial artificial chromosome DNA constructs into a variety of inbred and outbred mouse strains, including C57BL/6. (nyu.edu)
Locus2
One of the variant forms of a gene at a particular locus on a chromosome. (nih.gov)
We developed a conditional Bacterial Artificial Chromosome transgenic mouse (BACHD) that contains the entire human huntingtin genomic locus modified to include the Huntington's disease (HD) causing mutation. (nih.gov)
Genes6
F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. (wikipedia.org)
Bacteriophage lambda can pick up galactose genes from one bacterial cell and transfer them to another bacterium. (nih.gov)
However, due to the lack of TLR6 and 10 genes in teleost genomes and upregulation of TLR1 mRNA in response to LPS and bacterial infection in other fish species we hypothesize an important role for omTLR1 in antimicrobial immunity. (usda.gov)
At Regeneron Genetics Center ® , we've invented new robots and processes, even incorporated artificial intelligence and machine learning, to quickly sequence the genes of millions of people, analyze the data and find ways a person's genes can protect or expose them to disease. (regeneron.com)
Y linkage genes located on the Y sex chromosome, exhibiting holandric (q.v.) inheritance. (damasgate.com)
Linked bacterial artificial chromosome (BAC) clones will be subsequently sequenced to identify genes potentially related to mal. (anr.fr)
Contigs2
Die Analyse großer Cosmid- und BAC- (Bacterial Artificial Chromosome) Contigs, welche im Rahmen dieser Arbeit erstellt wurden und die mehr als 1 Mb sowohl des X- als auch des Y-Chromosoms des Platys X. maculatus abdecken, zeigte eine hohe Dichte von Retroelementen und anderen repetitiven Sequenzen, besonders im Bereich des dominanten Onkogens Xmrk und in einer durch das duplizierte Gen ps-criptY gekennzeichneten, Y-spezifischen Region. (uni-wuerzburg.de)
Using the results of pyrosequencing and assembling, we obtained six 5S rDNA- containing contigs with a total length of 140,417 bp, and two sets (pools) of individual 5S rDNA sequences belonging to separate, but closely located genomic regions on the 5BS chromosome. (biomedcentral.com)
Clones3
Comparative cytogenetic mapping was done using FISH with oligonucleotide probes and previously published chromosome-specific bacterial artificial chromosome (BAC) clones. (cas.cz)
Using pyrosequencing of BAC clones, in this work we studied the organization of two distinct 5S rDNA-tagged regions of the 5BS chromosome of bread wheat. (biomedcentral.com)
Three BAC-clones containing 5S rDNA were identified in the 5BS chromosome-specific BAC-library of Triticum aestivum . (biomedcentral.com)
Rainbow trout2
Every year, viral and bacterial epidemics in farmed Atlantic salmon and rainbow trout have resulted in production losses accounting for millions of dollars of lost revenue. (usda.gov)
Two TLR1 loci were identified from a rainbow trout bacterial artificial chromosome (BAC) library using DNA sequencing and genetic linkage analyses. (usda.gov)
Genomic2
Background: The bacterial artificial chromosome (BAC) is a vector system of choice for the construction of the large-insert chromosomal DNA libraries that are needed in genomic studies. (nih.gov)
A detailed structural organization of two closely located 5S rDNA-tagged genomic regions on the 5BS chromosome of bread wheat has been established. (biomedcentral.com)
Oligonucleotides1
Array CGH has also been used in MDS to comprehensively examine chromosome 5 using densely tiled oligonucleotides in a customized array platform. (medscape.com)
Probes4
Fluorescence in situ hybridization (FISH) analysis was performed with bacterial artificial chromosome (BAC) probes. (nih.gov)
In this study, chromosome-specific markers were used as probes for heterologous fluorescence in situ hybridization (FISH) to identify and characterize structural chromosome changes among the smooth-seeded (Lupinus angustifolius L., Lupinus cryptanthus Shuttlew. (cas.cz)
FISH using probes specific to both 5S rDNA unit types showed differences in the distribution and intensity of signals on the chromosomes of polyploid wheat species and their diploid progenitors. (biomedcentral.com)
Gene3
The hypothesis for the study was that disruption of a gene at one of the two breakpoints in the Rd4 chromosome is responsible for the retinal degeneration. (nih.gov)
In each mouse line, expression of the reporter or recombinase is controlled by promoter elements derived from a bacterial artificial chromosome (BAC) containing a specific gene of interest, in order to mimic the gene's expression pattern. (nih.gov)
These hybrids are coexpressed in a yeast strain lacking GAL4 activ- ity and containing a reporter gene (q.v.), such as the bacterial lac Z gene, with a binding site for GAL4. (damasgate.com)
Vector1
Additionally, the nonessential bacterial artificial chromosome vector was spontaneously deleted from both viral genomes upon passage in insects. (nerc.ac.uk)
Centromere1
yeast artificial chromosomes (YACs) genetically engineered circular chromosomes that contain ele- ments from chromosomes contributed by Saccharo- myces and segments of foreign DNAs that can be much larger than those accepted by conventional cloning vectors (q.v.). As shown in the diagram on page 480, YACs are generated from synthetic mini- chromosomes that contain a yeast centromere (C), a replication orgin (RO), and fused telomeres (TR and Tr). (damasgate.com)
Yeast2
Cosmid End-sequence profiling Fosmid Human artificial chromosome Secondary chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W (June 1989). (wikipedia.org)
Such artificial chromosomes are distributed normally dur- ing subsequent yeast divisions, and so colonies con- taining YACs are generated. (damasgate.com)
Library2
Goto T, Todo K, Miyamoto K, Akimoto S. Bacterial artificial chromosome library of Finegoldia magna ATCC 29328 for genetic mapping and comparative genomics. (medscape.com)
2005. Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics. (purdue.edu)
Bacteria2
A patient's recovery from anaerobic infection depends on prompt and proper treatment according to the following principles: (1) neutralizing toxins produced by anaerobes, (2) preventing local bacterial proliferation by changing the environment, and (3) limiting the spread of bacteria. (medscape.com)
DNA constructs that are composed of, at least, a REPLICATION ORIGIN , for successful replication, propagation to and maintenance as an extra chromosome in bacteria. (nih.gov)
Divisions1
Chromosome divisions and subdivisions are shown on the left sides of the idiograms. (biomedcentral.com)
Mouse1
The Rd4/+ mouse inherits an autosomal dominant retinal degeneration that cosegregates with a large inversion spanning nearly all of mouse chromosome 4 (Chr 4). (nih.gov)
Arms1
Chromosomes and chromosome arms are indicated by numbers 1, 2, and 3 and letters p and q, respectively. (biomedcentral.com)
System1
The development and applications of the bacterial artificial chromosome cloning system" (PDF). (wikipedia.org)
Engineering1
Herein, we show that the application of two strategies, cloning of the cDNAs into a bacterial artificial chromosome and nuclear expression of RNAs that are typically produced within the cytoplasm, is useful for the engineering of large RNA molecules. (nih.gov)
Found1
We found 1 global bacterial artificial chromosomes purification kit Tenders from the public procurement domain worldwide. (biddetail.com)
Quickly1
Lehrer described protegrins, a fascinating class of broad-spectrum antibiotics that quickly disrupt the bacterial outer membrane, killing cells within minutes. (biomedcentral.com)