AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
BiofilmsBacteriaGram-Negative BacteriaBacterial ProteinsBacterial AdhesionGram-Positive BacteriaPlanktonPseudomonas aeruginosaGene Expression Regulation, BacterialColony Count, MicrobialMicrobial ViabilityBacterial Physiological PhenomenaBacteria, AnaerobicAnti-Bacterial AgentsDNA, BacterialRNA, Ribosomal, 16SMicroscopy, Electron, ScanningMolecular Sequence DataQuorum SensingWater MicrobiologyStreptococcus mutansEscherichia coliCulture MediaPhylogenyBacteria, AerobicMicrobial Sensitivity TestsStaphylococcus aureusCandida albicansPolysaccharides, BacterialDNA, RibosomalMicroscopy, ConfocalStaphylococcus epidermidisSequence Analysis, DNAStreptococcus oralisMicrobial InteractionsDental PlaqueGenes, BacterialDisinfectantsRNA, BacterialMouthEquipment ContaminationBioreactorsBacteriological TechniquesAdhesins, BacterialSoil MicrobiologyCetylpyridiniumActinomycesBacterial LoadVirulenceAnaerobiosisMutationSeawaterAntibiosisFresh WaterPolystyrenesChlorhexidineSulfur-Reducing BacteriaFimbriae, BacterialDrug Resistance, BacterialStreptococcusProteobacteriaGram-Negative Aerobic BacteriaGentian VioletPseudomonasPlasma GasesGram-Negative Anaerobic BacteriaBiodegradation, EnvironmentalSpecies SpecificityFusobacterium nucleatumStainless SteelAnti-Infective Agents, LocalEnterococcus faecalisGenome, BacterialPolyvinyl ChlorideHomoserinePseudomonas fluorescensVirulence FactorsGene DeletionSymbiosisTobramycinMicrobial ConsortiaAntifungal AgentsPeracetic AcidStreptococcus gordoniiGenes, rRNAAerobiosisPseudoalteromonasAmino Acid SequenceVeillonellaBase SequenceSurface PropertiesBacterial InfectionsBioelectric Energy SourcesAnti-Infective AgentsBiomassEnvironmental MicrobiologyCatheter-Related InfectionsPseudomonas InfectionsPseudomonas putidaSilicone Elastomers
PseudomonasAnti-biofilmStaphylococcusExpressed genesDays of biofilm growthQuorumMicroorganismsStreptococcusPathogenic bacteriaColonizationInfectionsExtracellularMicrobesForm a biofilmFungiFormationAntimicrobialsAeruginosa biofilmBody's immune systemMicrobial cellsVirulenceMicroscopyNutrientsColoniesPlanktonic cellsAureusSurfacesCellsEffectivelyBacterial growthStrainsCariesVitroDispersalResistancePave the wayInhibitionPersistentSpeciesOral bacteriaProcessesDisruptAdditionallyGrowthCompoundsIntracellularPhenotypicSurfaceAbstractInfectionViable bacteriaPeptides
Pseudomonas12
Anti-biofilm8
  • The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
  • Additional screens identified 1018 as a potent anti-biofilm peptide that prevented biofilm formation and led to the eradication of mature biofilms in both Gram-negative and Gram-positive bacteria. (ubc.ca)
  • Further studies identified even more potent D-enantiomeric anti-biofilm peptides DJK-5 and DJK-6 that also prevented (p)ppGpp accumulation, were highly synergistic with conventional antibiotics and exhibited in vivo activity. (ubc.ca)
  • We hypothesize that the use of these anti-biofilm compounds in conjunction with an antibiotic will more effectively inhibit and disrupt Salmonella biofilms in vivo in our mouse model of chronic carriage when compared to the administration of antibiotic therapy alone. (hhs.gov)
  • In Aim 1, we will assess the efficacy of these anti-biofilm compounds at preventing and treating chronic infection compared to traditional antibiotics alone by utilizing our established mouse model of typhoidal chronic carriage. (hhs.gov)
  • In summary, we propose an investigation into the safety, efficacy, and mechanisms of novel anti-biofilm compounds to prevent and treat chronic infections by typhoidal Salmonella. (hhs.gov)
  • To our knowledge this study will be the first attempt (utilizing subject experts in anti-biofilm medicinal chemistry and Salmonella chronic infection) to specifically target Salmonella biofilm formation in vivo as a means of combating chronic carriage. (hhs.gov)
  • Inhibiting bacterial cooperation is an evolutionarily robust anti-biofilm strategy. (ox.ac.uk)
Staphylococcus2
Expressed genes1
  • The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
Days of biofilm growth2
  • Eliminating (p)ppGpp expression after 2 days of biofilm growth by removal of arabinose from a strain expressing relA behind an arabinose-inducible promoter, reciprocated the effect of peptide added at the same time, leading to loss of biofilm. (ubc.ca)
  • 20 generations, a few days of biofilm growth) within biofilms. (southampton.ac.uk)
Quorum4
  • We have [https://2009.igem.org/Team:SDU-Denmark/Background#Quorum-quenching_using_RIP reason to hope] that this will weaken the biofilm formation, thereby making multiresistant bacteria easier to treat with conventional antibiotics. (igem.org)
  • In biofilms, symbionts play major roles in the relationship among organisms by the production of bioactive molecules involved in quorum sensing signaling. (intechopen.com)
  • On the other hand, etrasimod was shown to inhibit quorum sensing (QS) signaling in Chromobacterium violaceum, suggesting that it may block the biofilm formation by targeting QS in certain Gram-negative bacteria. (helsinki.fi)
  • Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
Microorganisms9
  • A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
  • Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
  • The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
  • Biofilms are complex, highly dynamic, structured ecosystems formed by a community of different microorganisms living attached to inert or living surfaces and embedded in a matrix designated extracellular polymeric substance (EPS). (intechopen.com)
  • Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
  • or most of the history of microbiology, microorganisms in these biofilms was polysaccharide. (cdc.gov)
  • 5) in 1978 put forth a the- on exposed surfaces led to studies that revealed surface-associ- ory of biofilms that explained the mechanisms whereby micro- ated microorganisms (biofilms) exhibited a distinct phenotype organisms adhere to living and nonliving materials and the with respect to gene transcription and growth rate. (cdc.gov)
  • Since that time, the film microorganisms have been shown to elicit specific mech- studies of biofilms in industrial and ecologic settings and in anisms for initial attachment to a surface, development of a environments more relevant for public health have basically community structure and ecosystem, and detachment. (cdc.gov)
  • Van Leeuwenhoek, using his simple microscopes, first utilization of the confocal laser scanning microscope to char- observed microorganisms on tooth surfaces and can be cred- acterize biofilm ultrastructure, and an investigation of the ited with the discovery of microbial biofilms. (cdc.gov)
Streptococcus5
Pathogenic bacteria4
  • A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. (frontiersin.org)
  • However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
  • Activated lactoferrin (ALF) is a new form of a naturally occurring protein from milk that acts as a powerful deterrent to pathogenic bacteria that may be present on a meat surface. (ift.org)
  • Reference: Maisuria VB, Hosseinidoust Z, Tufenkji N. Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria. (uri.edu)
Colonization3
  • The second method is to inhibit the colonization of the oral bacteria, which involves surface antibacterial activity [ 7 ]. (hindawi.com)
  • Together these results provide a better understanding of the potential mechanisms of uptake and survival of various phenotypes of GAS bacteria relevant for colonization and recurrent infection. (lu.se)
  • Red propolis extract showed antibacterial activity against the tested strains, exhibited acceptable cytotoxicity and reduced the colonization of S. mutans and L. casei in a biofilm membrane model. (bvsalud.org)
Infections16
  • The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
  • Bacteria have evolved multiple strategies for causing infections that include producing virulence factors, undertaking motility, developing biofilms and invading host cells. (nature.com)
  • More than 65 percent of hospital-acquired infections manifest as biofilms. (sciencedaily.com)
  • Due to the resistance of some bacteria to antibiotics, research in the field of dealing with bacterial infections is necessary. (nature.com)
  • 3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)
  • 3,6 Biofilm formation by P. aeruginosa is responsible for antimicrobial tolerance and causes major problems for treatment of infections. (rsc.org)
  • 3,8 Therefore, it is important to develop biomaterials that can control biofilm growth thereby reduce infections. (rsc.org)
  • Understanding the biological characteristics of biofilms, the cause of most known bacterial infections, is the first step to fight against this silent pandemic and to find effective treatments. (ibecbarcelona.eu)
  • Bacterial infections are a public health problem affecting millions of people worldwid e, and about 80% of them are associated with biofilms , communities formed by different microorganism s' species which co-colonize human tissues and medical devices. (ibecbarcelona.eu)
  • Only with the continuous development and improvement of efficient antibiofilm strategies we can tackle the recurrence and chronicity caused by P. aeruginosa biofilm infections. (ibecbarcelona.eu)
  • It is also a relevant pathogen in chronic wounds infections and skin injuries , and in keratitis, a common corneal infection related with bacterial biofilm formation over contact lenses. (ibecbarcelona.eu)
  • Bacterial biofilms are formed by different species that colonize and grow together on human tissues, causing infections in the urinary tract, lung and kidney among others, and on surfaces of medical devices, as joint prostheses , heart valves and pacemakers, dental and breast implants, sutures and catheters. (ibecbarcelona.eu)
  • Bacteria form multicellular communities known as biofilms that cause two thirds of all infections and demonstrate increased adaptive resistance to conventional antibiotics. (ubc.ca)
  • Targeting biofilms represents a novel approach against drug-resistant bacterial infections. (ubc.ca)
  • Traditional antibiotic therapies are usually incapable of clearing chronic S. Typhi infections, as the biofilm phenotype renders the bacteria tolerant to the mechanisms of these drugs. (hhs.gov)
  • Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
Extracellular8
  • [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
  • This extracellular matrix glues bacteria to surfaces and serves as a slimy shield, protecting cells in the interior from predators and antibiotics. (the-scientist.com)
  • A cohesive structure of a multi‐layer of extracellular polymeric substances (EPS) such as polysaccharides and proteins is the base of biofilm structural organization. (intechopen.com)
  • Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)
  • This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)
  • Bacteria commonly form dense biofilms encased in extracellular polymeric substances (EPS). (ox.ac.uk)
  • Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down-regulation of specific genes. (cdc.gov)
  • Two major thrusts in the last decade cells that is enclosed in an extracellular polymeric substance have dramatically impacted our understanding of biofilms: the matrix. (cdc.gov)
Microbes8
  • Our working hypothesis was that some of the compounds that bacteria secrete might act more subtly, as signals to alter the behavior of their neighboring microbes rather than to kill them," said corresponding author Elizabeth A. Shank, PhD, assistant professor of biology, the University of North Carolina, Chapel Hill. (sciencedaily.com)
  • A biofilm is any group of microbes that stick together on a surface. (sciencedaily.com)
  • Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
  • Biofilms are home to millions of microbes, but disrupting their interactions could produce more effective antibiotics. (the-scientist.com)
  • These biofilms of aggregating microbes are everywhere: on rocks, bathtubs, and medical devices. (the-scientist.com)
  • The slimy coating on unbrushed teeth is biofilm, and the beneficial microbes on your skin and gut often grow collectively. (the-scientist.com)
  • Kovács's team has found that in populations of the soil bacterium Bacillus subtilis , most microbes assume responsibility for matrix production during early development when they are little more than a throng of unconnected cells. (the-scientist.com)
  • Furthermore, vinegar rinsing will destruct mature (24-h) biofilms, and significantly reduce the viability of planktonic microbes in saliva, thereby decreasing biofilm formation. (biomedcentral.com)
Form a biofilm1
  • Biofouling of gasoline can occur during fuel storage caused by bacteria and fungi that form a biofilm at a fuel/water interface and that produce organic acids and sulfides. (ornl.gov)
Fungi1
Formation16
  • For the study, first author Matthew Powers, an undergraduate student in Shank's lab, used a strain of B. subtilis--a species commonly used in lab experiments--that fluoresces when genes for biofilm formation are being shut off. (sciencedaily.com)
  • DAPG, or the DAPG-producing P. Protogens as a protobiotic, could be used to inhibit formation of harmful biofilms. (sciencedaily.com)
  • A practical approach utilized in this study involves the preparation of an antibacterial thin film on the surfaces, which can effectively inhibit and reduce biofilm formation and bacterial adherence. (nature.com)
  • If the native host bacteria win the race, tissue cells will not be able to displace these primary colonizers, and biofilm formation will occur developing into peri-implantitis [ 4 , 5 ]. (hindawi.com)
  • This study intended to elucidate the effect of commercially available vinegar on in situ pellicle formation and existing 24-h biofilms. (biomedcentral.com)
  • In situ biofilm formation took place on bovine enamel slabs mounted in individual splints and exposed intraorally over 3 min and 24 h, respectively. (biomedcentral.com)
  • The impact of vinegar on the initial microflora adherence to enamel and the formation of biofilm in situ have not yet been systematically analyzed. (biomedcentral.com)
  • Thus, the present study aims to investigate the effect of commercially available vinegar on in situ formation of the initial pellicle and on 24-h biofilms, as well as on salivary bacteria. (biomedcentral.com)
  • Biofilm formation has been found in 95% of patients intubated with endo-tracheal tubes for more than 24h. (ibecbarcelona.eu)
  • In this work, I first identified peptide 1037, which inhibited biofilm formation in a broad-spectrum manner and proposed that this activity might be due to the effect of the peptide on biofilm-associated processes. (ubc.ca)
  • Furthermore, relA and spoT mutations blocking production of (p)ppGpp replicated the effects of the peptide, leading to reduced biofilm formation. (ubc.ca)
  • An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
  • Thus, biofilm formation is a key step in the establishment of carriers. (hhs.gov)
  • Heukelekian and genes involved in cell adhesion and biofilm formation. (cdc.gov)
  • Cellulose membrane disks (13 mm, N=12) were used for biofilm formation (24 h) of S. mutans and L. casei , which were treated (1 min) with 0.4% RPE or 0.12% Chlorhexidine (CHX). (bvsalud.org)
  • The control group of biofilm formation was not submitted to any treatment. (bvsalud.org)
Antimicrobials3
  • Biofilms inhibit cleaning, prevent penetration of antimicrobials and provide a protected reservoir of bacteria. (vin.com)
  • In particular, surface modifications that physically create rational surface topographies have attracted attention in recent years, and have shown to inhibit bacterial attachment and biofilm growth without the use of antimicrobials. (rsc.org)
  • Biofilms are often extremely tolerant to antimicrobials but their reliance on shared EPS may also be a weakness as social evolution theory predicts that inhibiting shared traits can select against resistance. (ox.ac.uk)
Aeruginosa biofilm1
Body's immune system2
  • [13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
  • Maple syrup has amazing antimicrobial properties, which can help your body's immune system fight harmful bacteria. (uri.edu)
Microbial cells3
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
  • An estab- lished biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)
  • A biofilm is an assemblage of microbial cells that is irre- organisms could attach. (cdc.gov)
Virulence2
  • A significant part of the breast microbiota includes lactic acid bacteria, whose cell-free supernatant (LAB-CFS) may prevent bacteria from secreting virulence factors and lessen their pathogenicity ( 12 , 13 ). (frontiersin.org)
  • Here, live respiratory epithelial cells were inoculated with broth-grown or biofilm bacteria of different M-types, as well as with isogenic mutants lacking common virulence factors. (lu.se)
Microscopy4
  • In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. (microbiologyresearch.org)
  • Using fluorescence and scanning electron microscopy, bacteria were shown to align between the nanopillars. (rsc.org)
  • Microscopy of internalized bacteria showed that planktonic bacteria were internalized in lower numbers as individual or small clumps of bacteria in the cytoplasm, whereas GAS biofilm bacteria displayed a pattern of perinuclear localization of bacterial aggregates that affected actin structure. (lu.se)
  • 3) used scanning and transmission electron microscopy to ated organisms also differ from their planktonic (freely examine biofilms on trickling filters in a wastewater treatment suspended) counterparts with respect to the genes that are tran- plant and showed them to be composed of a variety of organ- scribed. (cdc.gov)
Nutrients2
  • Just as different boroughs of a city might differ in levels of air pollution or abundance of organic supermarkets, different parts of the biofilm are exposed to wildly different levels of oxygen and nutrients. (the-scientist.com)
  • These carbohydrates act as nutrients for biofilm bacteria, especially acidogenic and aciduric species. (bvsalud.org)
Colonies3
  • Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
  • Some bacteria create colonies called biofilms that can coat surfaces, reducing their effectiveness-for example, inhibiting a leaf's ability to photosynthesize. (asknature.org)
  • Research shows that not only do these maple extracts have the ability to shrink tumor cells, but they can significantly inhibit the growth of the biofilms, which are large colonies of bacteria. (uri.edu)
Planktonic cells1
  • Among them, etrasimod (APD334), an investigational drug for the treatment of ulcerative colitis, displayed the best inhibitory activity against S. aureus when growing as free-floating planktonic cells and within biofilms. (helsinki.fi)
Aureus7
  • This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
  • Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
  • The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. (frontiersin.org)
  • S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
  • Because of this, it is crucial to understand how LAB-CFS affects the makeup and function of metabolites in S. aureus biofilms. (frontiersin.org)
  • AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus . (dovepress.com)
  • It also displayed a potent activity against other Gram-positive bacteria, including penicillin- and methicillin-resistant S. aureus strains, S. epidermidis, and Enterococcus faecalis, with a minimum inhibitory concentration (MIC) ranging from 5 to 10 mu M (2.3-4.6 mu g/mL). (helsinki.fi)
Surfaces7
  • Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
  • The interaction of LF with microbial surfaces-OMPs of Gram-negative bacteria in particular-has led to other antimicrobial mechanisms, such as the inhibition of microbial attachment to sub-epithelial matrix proteins and detachment of bacteria from mucosal surfaces. (ift.org)
  • Biofilms can be found in a variety of habitats, on free‐living, on the surface of other organisms or inert surfaces, both in aquatic as well as terrestrial environments. (intechopen.com)
  • It is important to develop functional transmucosal implant surfaces that reduce the number of initially adhering bacteria and they need to be modified to improve the anti-bacterial performance. (hindawi.com)
  • Bacterial attachment is favoured on recessed portions of patterned surfaces, and bacteria tend to attach preferentially to patterns in the micro or nanometre range rather than to smooth surfaces. (rsc.org)
  • Zobell (2) observed that the number of versibly associated (not removed by gentle rinsing) with a sur- bacteria on surfaces was dramatically higher than in the sur- face and enclosed in a matrix of primarily polysaccharide rounding medium (in this case, seawater). (cdc.gov)
  • Biofilms may form on a wide variety of surfaces, isms (based on cell morphology). (cdc.gov)
Cells7
  • Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
  • They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
  • Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. (microbiologyresearch.org)
  • For instance, the cells within a B. subtilis biofilm that are engaged in protease production are randomly determined. (the-scientist.com)
  • Here we show that EPS of Salmonella biofilms is a cooperative trait whose benefit is shared among cells, and that EPS inhibition reduces both cell attachment and antimicrobial tolerance. (ox.ac.uk)
  • The M3 protein, but not the M1 or M5 proteins, was required for optimal uptake and persistence of both planktonic and biofilm bacteria inside cells. (lu.se)
  • osmium tetroxide fixative, these researchers were also able to The variable nature of biofilms can be illustrated from scan- show that the matrix material surrounding and enclosing cells ning electron micrographs of biofilms from an industrial water system and a medical device, respectively (Figures 1 and 2). (cdc.gov)
Effectively3
  • Quantification of AHL production using liquid chromatography-mass spectrometry confirmed that cerPAC effectively reduced the level of AHLs produced by the bacteria. (nature.com)
  • Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. (microbiologyresearch.org)
  • Also, the undiluted vinegar can effectively remove the bacteria from dentures, which will not cause oral mucosal damage even if residual vinegar remains on the denture [ 6 ]. (biomedcentral.com)
Bacterial growth2
  • Considered generally recognized as safe (GRAS) by the Food and Drug Administration and recently approved by the U.S. Dept. of Agriculture for use on fresh beef, ALF can be sprayed onto carcasses to help prevent bacterial contamination during processing or can be applied to a subprimal or finished beef surface prior to final packaging to inhibit bacterial growth and extend shelf life. (ift.org)
  • Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
Strains1
  • Interestingly, internalization and persistence of planktonic bacteria varied significantly between strains, whereas biofilm bacteria were internalized in similar and higher numbers, and all strains persisted beyond 44 hours, showing a more homogenous phenotype. (lu.se)
Caries2
  • Oral biofilm has been accepted to be the main reason of oral diseases, such as caries and periodontitis which are caused by dysbiosis and imbalance of the biofilm's composition [ 2 ]. (biomedcentral.com)
  • Dental caries results from surface demineralization caused by an organized biofilm exposed to fermentable carbohydrates from the diet [1]. (bvsalud.org)
Vitro4
  • The in vitro effect of manuka honeys on growth and adherence of oral bacteria. (microbiologyresearch.org)
  • Towards this end, our group has identified four small molecules and two antibodies capable of inhibiting and/or disrupting Salmonella biofilms in vitro. (hhs.gov)
  • We advance two of the small molecules in this proposal, JG-1 and M4, that both inhibit and disrupt biofilms in vitro and reduce GB bacterial numbers in vivo. (hhs.gov)
  • To evaluate in vitro the effect of a red propolis ethanolic extract (RPE) in the prevention of growth of a cariogenic biofilm and its cytotoxic potential. (bvsalud.org)
Dispersal3
  • Low levels of the peptide led to biofilm dispersal, while higher doses triggered biofilm cell death. (ubc.ca)
  • Nitric oxide-mediated regulation of biofilm dispersal. (southampton.ac.uk)
  • We discovered that exogenous nitric oxide can act as a signal for the dispersal of bacterial biofilms. (southampton.ac.uk)
Resistance4
  • Biofilms may also enhance the development of antimicrobial resistance - a gradual decrease in antimicrobial concentration means that some bacteria could be exposed to a mutant selection window. (vin.com)
  • Biofilms are notorious for their resistance to environmental stresses, including antimicrobial compounds. (southampton.ac.uk)
  • Treatment of biofilms with nitric oxide also reduced their resistance to antimicrobial compounds. (southampton.ac.uk)
  • In order to elucidate the mechanisms by which these compounds antagonize Salmonella biofilms, in Aim 2 we will identify the specific bacterial target(s) of each compound by enriching for mutants exhibiting compound resistance and by performing direct pull-downs of targets from bacterial lysates. (hhs.gov)
Pave the way2
  • 2,9-11 A comprehensive understanding of the interactions between bacteria and materials with different surface topographies may pave the way for more effective strategies to control biofilm growth. (rsc.org)
  • In this review, researchers decipher the implication of P . aeruginosa in biofilms and pave the way to find solutions in a near future. (ibecbarcelona.eu)
Inhibition1
  • This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)
Persistent2
  • Enter the bacteria… If a lactose intolerant bacteria sat down next to a northern European's lactase persistent bacteria (who had no problem drinking milk) and they shook hands, the lactose-digesting gene would immediately be transferred or shared with the lactose intolerant bacteria. (lifespa.com)
  • This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)
Species3
  • Bacteria are typically found as communities made up of several different species rather than as isolated, solitary organisms in nature ( 6 ). (frontiersin.org)
  • A known antibiotic and antifungal compound produced by a soil microbe can inhibit another species of microbe from forming biofilms - -microbial mats that frequently are medically harmful -- without killing that microbe. (sciencedaily.com)
  • In contrast to the free-living, or planktonic, bacteria commonly studied in the lab, most prokaryotes in nature settle down in complex communities, often consisting of several interacting species. (the-scientist.com)
Oral bacteria1
Processes4
  • Biofilms also interfere with industrial processes, for example, by clogging, or corroding pipes, and by instigating corrosion on ships' hulls. (sciencedaily.com)
  • Bacteria sense these microenvironments and adapt by up or down regulating the expression of certain genes to influence different cell processes. (the-scientist.com)
  • However, these processes are not conserved in bacteria and therefore did not explain the broad-spectrum activity of the peptide. (ubc.ca)
  • A greater understanding of biofilm processes should lead to novel, effective control strategies for biofilm control and a resulting improvement in patient management. (cdc.gov)
Disrupt1
  • Also, vinegar rinsing could reduce bacterial viability and disrupt the 24-h biofilm. (biomedcentral.com)
Additionally1
  • Additionally, the experimental approach could be used to discover other, potentially medically important biofilm-inhibiting bacterial, said Shank. (sciencedaily.com)
Growth3
  • [10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
  • Activated form of natural antimicrobial improves safety of beef and poultry by preventing attachment and growth of bacteria. (ift.org)
  • Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. (rsc.org)
Compounds2
  • Both of the bacteria from this study are associated with plant roots, and understanding their interactions using DAPG and other secreted compounds could be important for creating healthy microbial soil communities for plants to grow in, possibly boosting agricultural yields, said Shank. (sciencedaily.com)
  • My research group aims to understand how biofilms develop and disperse and how they respond to environmental stresses including therapeutic compounds. (southampton.ac.uk)
Intracellular2
  • Moreover, the high expression of capsule and SLO inhibited cellular uptake and capsule expression was required for intracellular survival. (lu.se)
  • Streptolysin S was required for optimal uptake and persistence of M3 planktonic bacteria, whereas SpeB improved intracellular survival of biofilm bacteria. (lu.se)
Phenotypic1
  • We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
Surface5
  • A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
  • [8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
  • These findings highlight that adding Cu to Ti can improve the antibacterial ability of the surface while inhibiting bacterial adherence. (nature.com)
  • Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
  • A biofilm is an assemblage of surface-associated microbial film characterization. (cdc.gov)
Abstract1
  • abstract = "New classes of antibiotics are urgently needed in the fight against multidrug-resistant bacteria. (helsinki.fi)
Infection1
  • GAS) is always of clinical significance in wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. (microbiologyresearch.org)
Viable bacteria1
  • In follow-up studies, etrasimod showed bactericidal activity and drastic reduction of viable bacteria within 1 h of exposure. (helsinki.fi)
Peptides1
  • We propose making a bandage that contains our engineered bacteria behind a semipermeable membrane, allowing only small peptides such as ''RIP'' to pass through, into the wound. (igem.org)